Labshake search
Citations for Stemcell Technologies :
801 - 850 of 1166 citations for IL 3 Human CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: Human embryonic or induced pluripotent stem cells were cultured in the commercially defined pluripotency maintaining media mTeSR1 (StemCell Technologies) on Cultrex (R&D Systems)-coated tissue culture plates ...
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bioRxiv - Immunology 2023Quote: ... T cells were obtained using a CD3+ T cell enrichment kit (EasySep™ Human T Cell enrichment, Stemcell Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: FACS purified single cell suspensions (500 cells/per well) were seeded in Human EpiCult-B media (Stem Cell Technologies) supplemented with 5 % FBS on day 0 ...
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bioRxiv - Bioengineering 2024Quote: ... CD4+ T cells were activated on-chip by adding ImmunoCult™ Human CD3/CD28 T Cell Activator (StemCell Technologies) to the culture media ...
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bioRxiv - Cancer Biology 2024Quote: ... PHGG and DMG cells were maintained in glioma growth medium (Human NeuroCult NS-A Proliferation kit (Stemcell Technologies, 05751) supplemented with 20 ng/ml human epidermal growth factor (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... using the EasySep™ Direct Human Neutrophil Isolation Kit according to the manufacturer’s instructions (StemCell Tech, Vancouver, BC, Canada). Each sorting resulted in more than 90% neutrophils ...
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bioRxiv - Immunology 2023Quote: Total monocytes were isolated by negative selection using the EasySep Human Monocyte Enrichment Kit without CD16 Depletion (StemCell Technologies) using the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... CVF was enriched for CD45+ cells using the EasySep™ Release Human CD45 Positive Selection Kit (Stem Cell Technologies) according to manufacturer protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... CD3+ T-cell populations were isolated using EasySep Human T Cell Isolation Kit (StemCell Technologies, Cat# 17951, lOT #1000153615). CD3+ T-cells were thawed ...
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bioRxiv - Cancer Biology 2024Quote: ... with base media consisting of Iscove’s modified Dulbecco’s medium (IMDM) supplemented with 2% human blood plasma (STEMCELL Technologies, #70039), 3% human AB serum (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... CD3 T cells were isolated by negative selection following manufacturer’s protocol (RosetteSep Human T cell enrichment cocktail, STEMCELL Technologies). Following isolation ...
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bioRxiv - Immunology 2024Quote: ... monocytes were enriched from cryopreserved PBMCs using EasySep™ Human Monocyte Enrichment Kit without CD16 Depletion Kit (STEMCELL technologies). Enriched monocytes were stimulated with 100ng/ml LPS for 4h at 37 °C before staining ...
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bioRxiv - Immunology 2024Quote: ... https://vi-talant.org/Home.aspx) by using the negative selection “RosetteSep Human NK Cell Enrichment Cocktail” kit (#15065; STEMCELL Technologies). Primary NK cells were cultured in CellGenix® GMP stem-cell growth media (SCGM ...
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bioRxiv - Immunology 2024Quote: ... Naïve CD8+ T cells were isolated by EasySep™ Human Naïve CD8+ T Cell Isolation Kit (STEMCELL Technologies, 19258). All isolated T cells were activated by CD3/CD28 Dynabeads (Thermo Fisher ...
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bioRxiv - Immunology 2024Quote: ... B cells were isolated from the samples using RosetteSep Human B cell Enrichment Cocktail (Stemcell Technologies, Vancouver, BC, Canada) following manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... T cells were obtained from whole blood using the RosetteSep™ Human T Cell Enrichment Cocktail (StemCell Technologies, 15061), followed by density gradient separation using Ficoll® Paque Plus (Cytiva Life Sciences ...
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bioRxiv - Cell Biology 2020Quote: ... then 300 µL of this solution was added in 3 mL of MethoCult TM GF3434 (STEMCELL Technologies) to obtain a final concentration of 104 cells ...
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bioRxiv - Cell Biology 2020Quote: ... plates at a concentration of 3×104 cells/cm2 in mTeSR1 medium (Stem Cell Technologies, Vancouver, CA) supplemented with 10μM ROCK inhibitor (Y-27632 ...
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bioRxiv - Neuroscience 2021Quote: ... and supplemented with 20 ng/ml BDNF and 20 ng/ml Neurotrophin-3 (NT3, #78074; STEMCELL Technologies) until day 43 ...
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bioRxiv - Neuroscience 2021Quote: ... approximately 3 × 106 single cells were added per well in the AggreWell 800 plate (STEMCELL Technologies, 34815) with Essential 8 flex medium supplemented with Stemgent hES Cell Cloning & Recovery Supplement (1X ...
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bioRxiv - Cell Biology 2020Quote: ... and transferred at 3 x 106 cells to a well of an AgrreWell800 plate (Stem Cell Technologies) in STEMdiff Neural Induction Medium + SMADi (Stem Cell Technologies ...
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bioRxiv - Cancer Biology 2021Quote: ... For the first 2 days cells were also exposed to 3 μM CHIR-99021 (Stem Cell Technologies). From day 4 for 5 days ...
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Single cell determination of cardiac microtissue structure and function using light sheet microscopybioRxiv - Bioengineering 2020Quote: ... plates at a concentration of 3×104 cells/cm2 in mTeSR1 medium (Stem Cell Technologies, Vancouver, CA) supplemented with 10µM ROCK inhibitor (Y-27632 ...
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bioRxiv - Microbiology 2020Quote: CD34+ HPCs from 3 independent donors were thawed and plated in StemSpan SFEM II media (Stemcell Technologies) containing 10% BIT serum substitute ...
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bioRxiv - Immunology 2022Quote: ... approximately 3×104 total Ai14 BM cells were plated per well in MethoCult (M3434, Stem Cell Technologies) enriched with rmSCF ...
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bioRxiv - Neuroscience 2023Quote: ... hiPSCs are cultured and passaged for 3 times in STEMdiff™ Neural Induction Medium + SMADi (Stemcell Technologies) for up to 3 weeks ...
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bioRxiv - Cell Biology 2023Quote: ... Sorted cells were plated in Methocult optimum media (2-3 X 103 cells/well; Stemcell Technologies, H4034) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... NK cell isolation was done by using the negative selection kit “RosetteSep™ Human NK Cell Enrichment Cocktail” (STEMCELL Technologies) according to the company’s protocol ...
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Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cellsbioRxiv - Microbiology 2020Quote: ... and T-cells were isolated via the Easy-Sep™ Rosette Human CD4+ T-cell enrichment kit (Stemcell Technologies, Canada) according to manufacturer’s protocols ...
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bioRxiv - Synthetic Biology 2019Quote: T cells were purified from normal donors by negative selection using the RosetteSep Human CD4+ T Cell Enrichment Cocktails according to the manufacturer’s protocols (StemCell Technologies). Primary cells were activated and expanded using Human T-activator CD3/CD28 Dynabeads (Cat # 11161D ...
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bioRxiv - Microbiology 2019Quote: Human monocytes were negatively selected from buffy coats using the RosetteSep Monocyte Enrichment Kit (1mL/10mL buffy coat; StemCell Technologies) and differentiated into moDC ...
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bioRxiv - Immunology 2019Quote: ... CD4+ T cells were further isolated through beads-mediated negative selection (EasySep Human CD4+ T cell Isolation Kit, Stemcell technology) and resuspended in Trizol (Ambion ...
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bioRxiv - Immunology 2019Quote: ... CD4+ T cell isolation was performed through beads-mediated negative selection (EasySep Human CD4+ T Cell Isolation Kit, Stemcell Technologies) and CD4+ cell purity was assessed by flow cytometry.
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bioRxiv - Genetics 2019Quote: ... lineage positive cells were depleted by negative selection with the StemSep Human Hematopoietic Progenitor Cell Enrichment Kit (StemCell Technologies, 14056) and Anti-Human CD41 TAC (Stem Cell ...
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bioRxiv - Bioengineering 2021Quote: ... CD8+ T cells were isolated from PBMCs by positive magnetic selection using EasySep Human CD8 Positive Selection Kit (StemCell Technologies). CD4+ T cells were isolated from PBMCs by negative magnetic selection using EasySep Human CD4 T cell Isolation Kit (StemCell Technologies) ...
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bioRxiv - Molecular Biology 2021Quote: ... Total CD4+ T cells were separated by negative selection using RosetteSep™ Human CD4+ T Cell Enrichment Cocktail (StemCell Technologies). Primary CD4+ T cells were plated at a concentration of 1,5×10 6 cells/mL left overnight for recovery and infected ...
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bioRxiv - Cancer Biology 2020Quote: ... CD34+ cells were isolated from umbilical cord blood using the EasySep™ Human CD34 Positive Selection Kit II (Stemcell Technologies). Cytapheresis of CML patients were kindly provided by Pr ...
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bioRxiv - Cell Biology 2021Quote: ... co-D12 or –D14 CD34+ cells were isolated using a human CD34 positive selection kit (Stem Cell Technologies, Vancouver, Canada). CD34+ cells (1 × 105/mL ...
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bioRxiv - Immunology 2022Quote: ... Neutrophils were obtained from peripheral blood of healthy voluntary donors using the EasySep Direct Human Neutrophil Isolation Kit (STEMCELL Technologies), following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... Tissues at 19 w and 20 w were subjected to EasySep™ Human CD45 Depletion Kit II (STEMCELL Technologies, 18259) to remove CD45+ immune cells ...
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bioRxiv - Cancer Biology 2022Quote: ... DNTs were enriched by depleting CD4+ and CD8+ cells using RosetteSep™ human CD4- and CD8-depletion cocktails (Stemcell Technologies). The CD4 and CD8 depleted cells were cultured in 24-well plates pre-coated with 5◻μg/ml anti-CD3 antibody (OKT3 ...
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bioRxiv - Cancer Biology 2020Quote: ... Isolated Lin− breast cancer cells were cultured in Human EpiCult™-B Medium Kit medium (Stem Cell Technologies, Vancouver, Canada) containing a supplement mix ...
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bioRxiv - Microbiology 2022Quote: ... Monocytes were isolated from whole blood by negative selection using RosetteSep human monocyte enrichment cocktail following the manufacturer’s protocol (StemCell Technologies). Using density gradient medium (Lymphoprep ...
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bioRxiv - Immunology 2019Quote: CD4+ T cells were obtained from PBMCs using the EasySep human CD4+ T cell enrichment kit (StemCell Technologies, Meylan, France). Next ...
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bioRxiv - Synthetic Biology 2020Quote: ... CD4+ and CD8+ T cells were isolated using the RosetteSep Human T cell enrichment cocktail (STEMCELL Technologies, 15022 and 15023), and PBMCs were isolated using Lymphoprep (STEMCELL Technologies ...
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bioRxiv - Bioengineering 2021Quote: ... total CD4+ T cells were isolated using a combination of the human CD4+ T cell RosetteSep™ kit (STEMCELL Technologies) and Ficoll-Paque (Cytiva Inc. ...
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bioRxiv - Immunology 2021Quote: CD14 positive cells were isolated from the PBMCs using the EasySep™ Human CD14 Positive Selection Kit by STEMCELL Technologies™ (Cat# 18058).
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bioRxiv - Developmental Biology 2019Quote: ... human iPSCs were seeded on a matrigel coated dish and cultured in the STEMdiff SMADi Neural Induction medium (STEMCELL Technologies) for 7 days34 ...
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bioRxiv - Cell Biology 2020Quote: For structured illumination microscopy primary NK cells were enriched from peripheral blood of healthy human donors using RosetteSep (Stemcell Technologies). Freshly isolated cells were pre-incubated with anti-CD56 Alexa Fluor 647 (clone HCD56 ...
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bioRxiv - Cancer Biology 2021Quote: ... CD8+ T cells were isolated from freshly-thawed PBMCs using the human CD8+ T cell isolation kit (Stem Cell Technologies). Anti-ILT3 52B8 (1µg/mL ...