Labshake search
Citations for Stemcell Technologies :
801 - 850 of 1846 citations for HLA A*0201 WT 1 complex Protein Human HEK293 His since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Human peripheral blood mononuclear cells (PBMCs) were isolated by density centrifugation from peripheral blood leukocyte separation medium (STEMCELL Technologies). In vitro experiments were performed using human plasmacytoid dendritic cells (pDCs ...
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bioRxiv - Immunology 2021Quote: ... 5mL of whole blood was enriched for CD4+ cells using RosetteSep Human CD4+ T Cell Enrichment Cocktail (StemCell Technology). CD4+ cells were frozen in 10% DMSO/FBS and stored in liquid nitrogen ...
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bioRxiv - Immunology 2020Quote: ... we treated CD4+ T cells with either αCD3αCD28 (ImmunoCult™ Human CD3/CD28 T Cell Activator; STEMCELL Technologies, 10971) or IFN I (1:1000 ...
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bioRxiv - Immunology 2020Quote: ... Isolated CD3+ cells and tumor-infiltrating lymphocytes (TILs) were maintained in Human T Cells Expansion (STEMCELL Technologies, Vancouver, Canada) medium supplemented with 5% of heat-inactivated human AB serum (Omega Scientific ...
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bioRxiv - Microbiology 2021Quote: ... and resting CD4+ T cells (rCD4s) (EasySep™ Human Resting CD4+ T Cell Isolation Kit, Cat # 17962, StemCell Technologies) were isolated from HIV-1 aviremic donors living with HIV-1 ...
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bioRxiv - Immunology 2022Quote: ... Total CD4 T cells from PBMCs were isolated using the EasySep Human CD4+ T cell enrichment kit (StemCell technologies). Prior to infection ...
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bioRxiv - Immunology 2022Quote: ... whereas the naïve B cells were isolated using the EasySep Human Naïve B cell isolation kit (Stem Cell Technologies). The purity of the B cells used in these experiments was > 95% ...
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bioRxiv - Molecular Biology 2022Quote: Myeloid cells were purified from PBMCs using Easy Sep Human Myeloid positive selection kit (Stem cell technology; Cat# 18653). The kit positively selects myeloid cells positive for CD33 and CD66b ...
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bioRxiv - Microbiology 2023Quote: ... CD4+ T cells were enriched using the EasySep Human CD4+ T Cell Enrichment Kit (STEMCELL Technologies, Cat. No. 19052) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... CD3+ T-cell populations were isolated using EasySep Human T Cell Isolation Kit (StemCell Technologies, Cat# 17951, lOT #1000153615). CD3+ T-cells were thawed ...
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bioRxiv - Developmental Biology 2024Quote: Human embryonic or induced pluripotent stem cells were cultured in the commercially defined pluripotency maintaining media mTeSR1 (StemCell Technologies) on Cultrex (R&D Systems)-coated tissue culture plates ...
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bioRxiv - Cancer Biology 2024Quote: ... PHGG and DMG cells were maintained in glioma growth medium (Human NeuroCult NS-A Proliferation kit (Stemcell Technologies, 05751) supplemented with 20 ng/ml human epidermal growth factor (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... with base media consisting of Iscove’s modified Dulbecco’s medium (IMDM) supplemented with 2% human blood plasma (STEMCELL Technologies, #70039), 3% human AB serum (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... CD3 T cells were isolated by negative selection following manufacturer’s protocol (RosetteSep Human T cell enrichment cocktail, STEMCELL Technologies). Following isolation ...
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bioRxiv - Immunology 2024Quote: ... monocytes were enriched from cryopreserved PBMCs using EasySep™ Human Monocyte Enrichment Kit without CD16 Depletion Kit (STEMCELL technologies). Enriched monocytes were stimulated with 100ng/ml LPS for 4h at 37 °C before staining ...
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bioRxiv - Immunology 2023Quote: ... T cells were obtained from whole blood using the RosetteSep™ Human T Cell Enrichment Cocktail (StemCell Technologies, 15061), followed by density gradient separation using Ficoll® Paque Plus (Cytiva Life Sciences ...
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bioRxiv - Immunology 2024Quote: ... Naïve CD8+ T cells were isolated by EasySep™ Human Naïve CD8+ T Cell Isolation Kit (STEMCELL Technologies, 19258). All isolated T cells were activated by CD3/CD28 Dynabeads (Thermo Fisher ...
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bioRxiv - Immunology 2024Quote: ... https://vi-talant.org/Home.aspx) by using the negative selection “RosetteSep Human NK Cell Enrichment Cocktail” kit (#15065; STEMCELL Technologies). Primary NK cells were cultured in CellGenix® GMP stem-cell growth media (SCGM ...
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bioRxiv - Immunology 2024Quote: ... B cells were isolated from the samples using RosetteSep Human B cell Enrichment Cocktail (Stemcell Technologies, Vancouver, BC, Canada) following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... CD4+ T cells were activated on-chip by adding ImmunoCult™ Human CD3/CD28 T Cell Activator (StemCell Technologies) to the culture media ...
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bioRxiv - Developmental Biology 2023Quote: ... iPSCs were cultured on recombinant human VTN (rhVTN) coated 60mm plates in StemFlex media (Stem Cell Technologies, cat. A3349401) and passaged using CTS EDTA Versene Solution (Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... cells from healthy donor and HSCT recipients were labeled with Rosettesep human monocyte enrichment cocktail (Stemcell Technologies, Cambridge, MA) and centrifuged through Histopaque density gradient medium (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... CD4+ T cells were isolated from monocyte-depleted PBMCs using EasySepTM Human CD4+ Selection Kit II (StemCell Technologies Ltd.) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Human PBMCs from healthy donors were isolated by density gradient method using Ficoll-Paque and Sepmate tubes (Stemcell Technology). Live naïve CD4+ T cells were purified from PBMCs post staining with fluorescence labelled antibodies to human CD3(OKT3 ...
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bioRxiv - Developmental Biology 2023Quote: ... Mammary organoids from eutherian species were cultured in Intesticult human organoid growth media (StemCell Technologies, Vancouver, Canada, Cat# 06010). Mammary organoids of opossum were cultured in either Intesticult ...
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bioRxiv - Immunology 2023Quote: NK Cells were enriched from freshly isolated PBMC using the EasySep™ Human NK Cell Isolation Kit (STEMCELL Technologies). NK cells were then counted and resuspended at 1M/mL in RPMI-1640 supplemented with 10% fetal bovine serum ...
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bioRxiv - Cancer Biology 2022Quote: ... were seeded (70,000 cells each) in medium for long-term culture of human cells (catalogue no. 05150; STEMCELL Technologies) supplemented with hydrocortisone in collagen-coated plates ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% heat-inactivated FCS and stimulated with Immunocult™ Human CD3/CD28 T cell Activator (Stemcell Technologies) (25 µL/ 106 cells ...
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bioRxiv - Microbiology 2023Quote: In vitro IL-10 assays were also performed using human Peripheral Blood Mononuclear Cells (PBMCs) (STEMCELL Technologies, Cat. # 70025.1). The assay was performed as described above ...
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bioRxiv - Immunology 2023Quote: ... B cells were extracted by negative selection with the EasySep™ Human B Cell Isolation Kit (StemCell Technologies, #17954). EBV-containing supernatants from B95-8 cells were added to purified B cells supported by 30 µg mL-1 holo-transferrin and 2.5 µg mL-1 CpG (ODN2006) ...
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bioRxiv - Immunology 2023Quote: Human neutrophils were purified from blood of healthy volunteers with the EasySep neutrophil isolation kit (StemCell Technologies, Vancouver, Canada) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: Transduced T cells were depleted of CD56 cells using the EasySep Human CD56 positive selection kit (Stemcell Technologies; 17855) and rested for 24 hours ...
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bioRxiv - Biophysics 2023Quote: ... Cells are cultivated in Epicult basal medium with Supplement C (Epicult-C human media kit, Stem Cell Technologies, Vancouver), 5% FCS ...
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bioRxiv - Neuroscience 2023Quote: Human iPSCs at low passage number were cultured in mTeSR medium (STEMCELL Technologies; Vancouver, BC, Canada; Cat. No. 85850) in 10-cm culture dishes (Thermo-Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... using the EasySep™ Direct Human Neutrophil Isolation Kit according to the manufacturer’s instructions (StemCell Tech, Vancouver, BC, Canada). Each sorting resulted in more than 90% neutrophils ...
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bioRxiv - Immunology 2023Quote: Total monocytes were isolated by negative selection using the EasySep Human Monocyte Enrichment Kit without CD16 Depletion (StemCell Technologies) using the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: FACS purified single cell suspensions (500 cells/per well) were seeded in Human EpiCult-B media (Stem Cell Technologies) supplemented with 5 % FBS on day 0 ...
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bioRxiv - Immunology 2024Quote: ... CD4 and CD8 T cells were isolated separately using EasySep Human CD4+ or CD8+ Enrichment Kits (Stem Cell Technologies). Isolated T cells were immediately activated with DynaBeads Human T-Activator CD3/CD28 (ThermoFisher) ...
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bioRxiv - Immunology 2024Quote: ... followed by depletion of non-NK lineages using immuno-magnetic beads (EasySep human NK cell enrichment kit, Stemcell Technologies). Samples were sequenced in dedicated flow cells on a HiSeq 2500 (Illumina ...
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bioRxiv - Immunology 2024Quote: ... Human whole-blood samples were diluted with 2% FBS in PBS prior to gradient centrifugation on Lymphoprep (StemCell Technologies). A percentage of the freshly isolated PBMCs was labelled with anti-human CD3 ...
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bioRxiv - Immunology 2024Quote: ... after which bulk T cells T cells were enriched using EasySep Human T Cell Enrichment Kits (Stem Cell Technologies), then maintained in RPMI-1640 (ATCC ...
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bioRxiv - Bioengineering 2024Quote: ... (i) Dextran-coated MNPs and anti-CD4 antibodies (EasySep Human CD4 Positive Selection Kit II, STEMCELL Technologies Canada Inc.) were incubated at a 1:1 ratio over night at 3 – 8 °C ...
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bioRxiv - Cell Biology 2024Quote: ... H1 human embryonic stem cells (WiCell, WA01) were routinely cultured as colonies in mTeSR Plus (STEMCELL Technologies, 100-0276) with 0.2% penicillin/streptomycin (BioConcept ...
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bioRxiv - Immunology 2024Quote: ... T cells were obtained using a CD3+ T cell enrichment kit (EasySep™ Human T Cell enrichment, Stemcell Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2024Quote: ... Organoids were expanded fed every other day with complete Intesticult Organoid Growth Medium (OGM, Human, StemCell Technologies, Cat # 06010), supplemented with 10µM Rho Kinase inhibitor Y-27632 (Tocris ...
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bioRxiv - Cell Biology 2024Quote: ... Monocytes were then enriched from the PBMC samples via the EasySep Human Monocyte Enrichment Kit (StemCell Technologies Inc, #19059). Monocytes were seeded at a concentration of 500.000 cells/mL in blood cell growth media (Sigma-Aldrich ...
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A non-invasive method to sample immune cells in the lower female genital tract using menstrual discsbioRxiv - Immunology 2024Quote: ... CVF was enriched for CD45+ cells using the EasySep™ Release Human CD45 Positive Selection Kit (Stem Cell Technologies) according to manufacturer protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Two million human tumor cells were cultured under mammosphere conditions using 5 mL of complete Mammocult medium (StemCell Technologies) in ultra-low attachment plates (Corning ...
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bioRxiv - Cancer Biology 2024Quote: ... 12 ng/mL human-bFGF (#78006) and 2 ug/mL heparin solution 0.2% (#07980) from Stemcell Technologies (Vancouver, Canada). Neurospheres were dissociated using Accutase (#00-4555-56 ...
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bioRxiv - Cancer Biology 2024Quote: ... transduced cells were isolated with the EasySep Human CD271 Positive Selection Kit II with EasyEights EasySep Magnets (StemCell Tech.) according to the manufacturer protocol ...