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Citations for Stemcell Technologies :
801 - 850 of 2055 citations for Estriol ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... with EasySep Human T cell Isolation Kit (STEMCELL Technologies). Cells were then activated for 3 days in “Complete media” (Immunocult-XF T cell expansion media (STEMCELL Technologies) ...
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bioRxiv - Neuroscience 2023Quote: ... StemDiff Neural Crest Differentiation Kit (StemCell Technologies catalog #08610) was used according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... using the mouse T cell isolation kit (StemCell Technologies), and following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... and EasySep APC Positive Selection Kit II (StemCell Technologies) following manufacturers recommendations.
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bioRxiv - Immunology 2023Quote: ... and EasySep APC Positive Selection Kit II (StemCell Technologies) following manufacturers recommendations.
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bioRxiv - Immunology 2023Quote: ... An EasySep™ Isolation Kit (19765, StemCell Technologies, Canada) was used to isolate naïve CD4+ T cells ...
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bioRxiv - Immunology 2023Quote: ... The EasySep PE Positive Selection Kit II (StemCell Technologies) and MojoSort Magnets (BioLegend ...
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bioRxiv - Cell Biology 2023Quote: ... ACF Cell Dissociation Kit (Stem Cell Technologies; Catalogue #05426) was used for passaging the MSCs for expansion and experiments ...
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bioRxiv - Immunology 2024Quote: ... or Human T Cell Enrichment Kits (Stem Cell Technologies) and cultured in RPMI 1640 (ATCC ...
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bioRxiv - Immunology 2024Quote: ... and the Mouse Streptavidin RapidSpheres Isolation kit (Stemcell Technologies). 1.25 x 106 CFSE-labeled splenic T cells (enriched as above ...
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bioRxiv - Cell Biology 2023Quote: ... intestinal epithelial organoid culture kit protocol (Stem cell technologies) was followed as per the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... CD3+ T cells or CD56+CD3-NK cells were isolated from the PBMC population using the EasySep Human T Cell Isolation Kit or EasySep Human NK Cell Isolation Kit (STEMCELL Technologies, Cambridge, MA). T cells were frozen at 1-2 × 107 cells/mL and NK cells were frozen at 5 × 106 cells/mL in CryoStor CS10 (STEMCELL Technologies ...
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bioRxiv - Bioengineering 2021Quote: ... enriched lineage negative cells were further purified to obtain an enriched Lin-Sca-1+ c-kit+ (LSK) population using EasySep™ Mouse Sca1 and cKit Positive Selection kits (#18756 & #18757, Stemcell Technologies, CA). For ROS studies ...
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bioRxiv - Immunology 2019Quote: ... naïve and memory CD4+ T cells were isolated from PBMCs using EasySep® naïve CD4+ T cell isolation kit and memory CD4+ T cell enrichment kit (StemCell Technologies, Meylan, France) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Single cell suspensions were prepared from multiple OT-I and OT-I;IL-7Rα449F mice spleens and CD8 T cells were purified using the CD8 T cell negative selection kit (EasySep™ Mouse CD8+ T Cell Isolation Kit) from Stem Cell Technologies. 1×106 cells were transferred I.V ...
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bioRxiv - Genomics 2021Quote: ... CD4+ T cells were isolated from the PBMC fraction using EasySep® naïve CD4+ T cell isolation kits and memory CD4+ T cell enrichment kits (StemCell Technologies, Meylan, France) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... CD8+ and CD3+ T cells were isolated from PBMCs using the EasySep™ Human CD8+ T Cell Isolation Kit and EasySep™ Human T Cell Isolation Kit (both from STEMCELL Technologies), respectively ...
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bioRxiv - Neuroscience 2023Quote: Cerebral organoids were generated using the STEMdiff™ Cerebral Organoid Kit and STEMdiff™ Cerebral Organoid Maturation Kit (Cat# 08570 & 08571, STEMCELL Technologies) according to the manufacturer’s instructions with the following modifications ...
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bioRxiv - Developmental Biology 2024Quote: ... quality control tests were routinely performed on hESC cultures, using a human stem cell pluripotency detection qPCR kit (Sciencecell, 0853) and hPSC genetic analysis kit (Stem Cell Technologies, 07550), according to the manufacturer’s protocols.
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bioRxiv - Cancer Biology 2019Quote: Fresh or culture progeny from total BM were counted and 5000 cells/well of a 24-well plate were resuspended in Methocult (M3434, Stem Cell Technologies, Vancouver, BC, Canada). Colonies were counted at day 7 ...
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bioRxiv - Cell Biology 2023Quote: ... and the picked colonies were allowed to grow larger and they ‘passaged’ by mechanical splitting into a new Matrigel coated plate containing mTeSR™1 media with its supplement(Anonymous, n.d.) and maintained as suggested by the manufacturer (StemCell Technologies, Canada Cat. No. #85850). Subsequent passages were done by enzymatic dissociation (Accutase® ...
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bioRxiv - Cancer Biology 2021Quote: ... Patient sample mononuclear cells were cultured in RPMI with 10% FBS and 50% HS-5 conditioned media (ATCC) or SFEMII supplemented with 1x StemSpan CD34+ Expansion Media and 1 uM UM729 (StemCell Technologies).
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bioRxiv - Genomics 2020Quote: ... H1 cells were daily fed with fresh mTeSR1 media and passaged every 4-5 days using ReLeSR reagent (StemCell Technologies, 05872). Cells were dissociated into single cells with TrypLE Express (Thermo Fisher ...
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bioRxiv - Neuroscience 2021Quote: ... Suspension was mixed with 250 ul of 50% Iodixanol solution (25 mM KCl, 5 mM MgCl2, 10 mM Tris pH 8.0, 50% Iodixanol (60% stock from STEMCELL Technologies, 7820), 1x protease inhibitor (Roche ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were initially dissociated into single cell suspensions by accutase treatment (Stemcell Technologies, 07920, 30-60 min, 37 °C, 5% CO2) and then fixed and permeabilized using fixation/permeabilization buffers (Foxp3/Transcription Factor Staining Buffer Set ...
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bioRxiv - Immunology 2021Quote: Bone marrow colony forming assay was performed by plating 104 or 5×104 cells on methylcellulose (MethoCult M3434, Stem Cell Technologies). GEMM (Granulocyte/Erythrocyte/Macrophage/Megakaryocyte) ...
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bioRxiv - Immunology 2019Quote: ... Cells were then permeabilized with 0.3% Triton-X for 15 min followed by 30 min block in 0.1% Triton-X + 5% normal rat serum (Stem Cell Technologies). Cells were incubated in Drp1 p616 Ab overnight at 4°C in 0.1% Triton-X + 1% BSA and then in secondary antibodies (AF488 Goat anti-Rabbit) ...
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Parallelized engineering of mutational models using piggyBac transposon delivery of CRISPR librariesbioRxiv - Bioengineering 2020Quote: ... cells were maintained on 6-well plates in a humidified incubator at 37°C with 5% CO2 and passaged every 3-4 days (at ∼80% confluence) using ReLeSR (Stemcell Technologies), following the manufacturer’s protocol ...
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bioRxiv - Immunology 2019Quote: Cells were cultured at 37°C under 5% CO2 in 100 μl of StemSpan serum-free expansion medium (STEMCELL technologies 9650) supplemented with cytokine cocktails aimed at proliferation (50 ng/ml murine recombinant thrombopoietin (TPO ...
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bioRxiv - Genetics 2019Quote: ... the 0.2% Gelatin is removed and murine MS-5 stroma cells32 were seeded at a density of 1500 cells per well in 100μl H5100 media (StemCell Technologies, 05150). One-day prior ...
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bioRxiv - Genomics 2020Quote: Unsorted floating cells were taken from d2-3 of the blast culture and 5×103 cells were seeded in 1 ml MethoCult (M3434 STEMCELL Technologies) per dish ...
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bioRxiv - Developmental Biology 2021Quote: ... were plated at a density of ∼5 million neurons per cm2 in complete neuronal media recommended by the vendor as follows: BrainPhys Neuronal Medium (05790, STEMCELL Technologies) supplemented with iCell Neural Supplement B (M1029 ...
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bioRxiv - Neuroscience 2021Quote: ... 2.5 mL of cell suspension was transferred to a sterile 5 mL round-bottom flow cytometry tube and placed in the EasySep magnet (STEMCELL Technologies) for 5 min ...
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bioRxiv - Neuroscience 2021Quote: ... The media was changed daily and the iPSC cultures were split into 1:6-1:10 every 5 days using ReLeSR (Stemcell Technologies).
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bioRxiv - Neuroscience 2021Quote: ... centrifuged at 200 x g for 5 min prior to be resuspended in STEMdiff™ Neural Progenitor Medium (Stem Cell Technologies) and seeded onto growth-factor-reduced Matrigel™-coated plates (8.6µg/cm2 ...
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bioRxiv - Neuroscience 2021Quote: ... with 10 μM Y-23632 (only for the initial 24 hours; Wako) and subcultured every 5–7 days using ReLeSR reagent (STEMCELL Technologies).
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bioRxiv - Cell Biology 2021Quote: ... H9-hESC SOX10::GFP reporter and H20961 iPSC were passaged ∼1:6 every 4-5 days by incubating them in ReLeSR (StemCell technologies) for 1 min at room temperature ...
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bioRxiv - Developmental Biology 2020Quote: ... The Geltrex was allowed to set for 40 minutes in a 37°C humidified incubator with 5% CO2 before being submerged in IntestiCult media (STEMCELL technologies).
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bioRxiv - Developmental Biology 2022Quote: ... they were specified into a hiPSC-CM/stromal cell mixture using RPMI B27-containing 5 µM IWP-4 (Stem Cell Technologies) followed by another 7 days of RPMI B27+ (RPMI1640 GlutaMAX + 2% B27 supplement with insulin ...
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bioRxiv - Developmental Biology 2019Quote: ... All cell lines were cultured in humidified incubators (37 C and 5% CO2) using TeSR-E8 media (StemCell Technologies, Vancouver, Canada) or STEMdiff Neural Induction Medium (StemCell Technologies ...
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bioRxiv - Bioengineering 2021Quote: 5 wt% GelMAL-peptide and 5 wt% GelMA hydrogels containing 105 LSK/mL were immediately placed in 500 µL SFEM media (#09650, Stemcell Technologies) supplemented with 100 ng/mL SCF (#250-03 ...
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bioRxiv - Bioengineering 2021Quote: ... at 5×105 LSK/mL and cultured for 2 h at 5% CO2 and 37 °C in 500 µL SFEM media (#09650, Stemcell Technologies) supplemented with 100 ng/mL SCF (#250-03 ...
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bioRxiv - Bioengineering 2021Quote: ... Cells (∼20 x 105 cells/cm2) were replated using the medium in which they were last cultured plus 5 μM ROCK inhibitor (#Y-27632, StemCell Technologies) and 5% Gibco™ KnockOut™ Serum Replacement (KSR ...
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bioRxiv - Cell Biology 2020Quote: ... The dissociated glands were spun down at 450 x g for 5 min and resuspended in ammonium chloride solution (STEMCELL Technologies) diluted (4:1 ...
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bioRxiv - Cancer Biology 2021Quote: IL-10 polarized human DCs (3×10^4/well) were incubated with allogeneic CD8+ T cells (1.5 × 10^5/well) in T cell media (Stem Cell Technologies) in 96 well flat bottom tissue culture plates for 4 days ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were cultured in a Cytomat 24 (Thermo Fischer Scientific) at 37°C and 5% CO2 in mTeSR1 medium with and without phenol red (STEMCELL Technologies), supplemented with 1% penicillin-streptomycin (Thermo Fischer Scientific).
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bioRxiv - Bioengineering 2020Quote: ... the hiPSCs were collected and seeded at 1.5 × 105 cells/cm2 cell density on glass coverslips with Rho-associated coiled-coil containing protein kinase (ROCK) inhibitor supplemented mTeSR media (5 μM, StemCell Technologies). The culture was maintained with daily media changes until 90% confluency was reached ...
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bioRxiv - Bioengineering 2020Quote: ... and cells were encapsulated in 5 mm diameter hydrogels (20 µL) and cultured for 7 days in 300 µL SFEM media (#09650 Stemcell Technologies) supplemented with 100 ng/mL SCF (#250-03 ...
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bioRxiv - Cancer Biology 2022Quote: ... Patient samples were cultured in RPMI with 10% FBS and 10% HS-5 conditioned media (ATCC) or SFEMII supplemented with 1x StemSpan CD34+ Expansion Media and 1 µM UM729 (StemCell Technologies).
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bioRxiv - Cell Biology 2022Quote: ... then gently detached with Accutase and reseeded as 2-5×104 cells/cm2 in mTeSR medium containing 10uM Y-27632 (#72304, STEMCELL Technologies) ROCK inhibitor (removed after 48h and exchanged for fresh medium without ROCK inhibitor) ...