Labshake search
Citations for Stemcell Technologies :
801 - 850 of 1392 citations for Anti FLAG M5 SAP Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... CD8- thymocytes were obtained by negative selection using the EasySep Mouse Streptavidin Rapid Spheres Isolation Kit (Stem Cell Technologies), then subjected to cell sorting ...
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bioRxiv - Bioengineering 2021Quote: ... Reprogramming of erythroid progenitor cells was performed using the Erythroid Progenitor Reprogramming Kit following manufacturer’s instructions (Stem Cell Technologies). Briefly ...
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bioRxiv - Neuroscience 2020Quote: Differentiation into cerebral organoids was performed with a commercially available differentiation kit from Stemcell Technologies (Catalog number 08570-1) based on previously reported protocols [8 ...
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bioRxiv - Cancer Biology 2020Quote: ... Splenocytes were enriched in CD19+ cells using EasySep™ Mouse Pan-B Cell Isolation Kit (Stemcell Technologies, Vancouver, Canada) yielding a purity above 95% of CD5+ CD19+ cells ...
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bioRxiv - Cancer Biology 2020Quote: Both CD4+ and CD8+ cells were isolated from normal mouse splenocytes by immune-magnetic negative selection kit (Stemcell Technology, Vancouver ...
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bioRxiv - Immunology 2021Quote: ESC or iPSC were differentiated to hematopoietic progenitors following the manufacturer’s instructions for the STEMdiff Hematopoietic Kit (STEMCELL Technologies). Briefly ...
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bioRxiv - Neuroscience 2021Quote: ... The differentiation of neural progenitor cells (NPCs) was induced by STEMdiff SMADi Neural induction kit from STEM Cell Technologies. The differentiation of astrocytes was initiated using the Astrocyte medium (ScienCell ...
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bioRxiv - Immunology 2022Quote: ... White blood cells were isolated with the Erythroclear Red Blood Cell Depletion Reagent Kit (STEMCELL Technologies; cat. no. 01738).
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bioRxiv - Immunology 2022Quote: Isolation of neutrophils was carried out EasySep™ Direct Human Neutrophil Isolation Kit (Stem Cell Technologies, Vancouver, Canada, 19666), an immunomagnetic negative selection kit ...
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bioRxiv - Immunology 2020Quote: ... splenic NK cells were enriched to >80% purity using the EasySep Mouse NK Cell Isolation Kit (Stem Cell Technologies), and 1.0-2.5 x 104 NK cells were cultured in K10 media along with either IL-15 alone (1-100 ng/mL ...
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bioRxiv - Immunology 2020Quote: ... T cells were purified from thawed PBMCs using the EasySep Human T cell enrichment kit (STEMCELL Technologies, Cambridge, MA). RNA was extracted from purified human T cells using the RNeasy Plus Minikit (Qiagen ...
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bioRxiv - Cell Biology 2020Quote: ... medium was replaced with STEMdiff™ Hematopoietic Supplement B medium from the STEMdiff™ Hematopoietic kit (# 05310; STEMCELL technologies). Cells remained in this medium for 7 additional days ...
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bioRxiv - Immunology 2022Quote: ... CD4+ Human T cells were enriched from PBMCs using EasySep™ Human CD4+ T Cell Isolation Kit (StemCell Technologies) according to the manufacturers protocol ...
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bioRxiv - Immunology 2022Quote: ... Naïve splenic B lymphocytes were purified by negative selection using the EasySep Mouse B Cell Isolation Kit (STEMCELL Technologies). Primary B cells from each transgenic mouse line were cultured in RPMI 1640 with L-glutamine supplemented with 10% fetal bovine serum ...
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bioRxiv - Cancer Biology 2022Quote: ... Splenocytes derived from OT1 mice were prepared as described above and naïve CD8+ T cells were isolated using EasySepTM Mouse Naïve CD8+ T cell isolation kit (STEMCELL Technologies) following manufacturer’s instructions.
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bioRxiv - Cancer Biology 2019Quote: ... A crude enrichment for human cells was performed using an EasySep™ Mouse/Human Chimera Isolation Kit (STEMCELL Technologies) as per manufacturer instructions but with only a single separation on the magnet and with an extra wash of the mouse cells retained on the magnet during the pour off ...
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bioRxiv - Synthetic Biology 2019Quote: ... and magnetic bead selection using an EasySep™ Human CD34 Positive Selection Kit (STEMCELL Technologies Inc., Vancouver, BC, Canada). The resulting CD34+ HSPCs were cultured in StemSpan SFEM II (STEMCELL Technologies Inc. ...
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bioRxiv - Immunology 2019Quote: ... B cells were enriched from the SCS using Mouse Pan-B Cell Isolation kits (Stemcell Technologies, Vancouver, BC, Canada). Briefly ...
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bioRxiv - Immunology 2020Quote: ... plasma cells were isolated using the EasySep Human CD138 Positive Selection Kit II for future studies (Stemcell Technologies, #17877). The negative fraction of the above selections was divided into two aliquots that were subjected to negative immunomagnetic isolation of either B cells (EasySep Human Pan-B cell Enrichment Kit ...
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bioRxiv - Cell Biology 2019Quote: ... Human neutrophils were isolated within 1h after drawn using the human neutrophil direct isolation kit (STEMcell Technologies, Vancouver, Canada) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... Enrichment of T cells was performed by EasySep™ mouse T cell isolation kit per manufacturer’s protocol (StemCell Technologies). Retroviral transduction with PSCA-mCAR and/or ffluc and subsequent expansion were performed as previously described.47
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bioRxiv - Genetics 2021Quote: ... we performed immunohistochemistry in 11-week-old human cerebral organoids generated with the STEMdiff cerebral organoid kit (STEMCELL Technologies), and C57BL/6J mouse brains (Jackson laboratory ...
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bioRxiv - Immunology 2021Quote: ... Thy1.1+ cells were positively enriched using the EasySep™ PE Positive Selection Kit (Stem Cell Technologies, Cat no. 17684) or EasySep™ Release Mouse PE Positive Selection Kit (Stem Cell Technologies ...
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bioRxiv - Immunology 2020Quote: CD4+T-cells were isolated from PBMCs with the EasySep™ human CD4+T-cell enrichment kit (StemCell Technologies). T-cells/PBMCs were added to cultured hepatic epithelial cell lines (Huh-7 ...
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bioRxiv - Genomics 2019Quote: ... CD4+ T cells were enriched by negative selection using the EasySep Human CD4+ T Cell Enrichment Kit (StemCell Technologies). The purity of the CD4+ T cells was evaluated by flow cytometry and was higher than 95% ...
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bioRxiv - Immunology 2019Quote: ... OT-I thymocytes were depleted of mature CD8 single positives using the EasySep Biotin Positive Selection Kit (Stemcell Technologies) with anti-human/mouse β7 integrin antibody (FIB504 ...
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bioRxiv - Immunology 2019Quote: OT2 T cells were isolated by magnetic beads (EasySep™ Mouse Naïve CD4+ T Cell Isolation Kit, StemCell Technologies) from a single cell suspension of splenocytes ...
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bioRxiv - Cell Biology 2020Quote: Cord blood (CB) CD34+ cells were purified with Human Cord Blood CD34 Positive Selection Kit II (StemCell Technologies, 17896) and cultured in SFEM II medium (StemCell Technologies ...
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bioRxiv - Immunology 2020Quote: ... and used directly for total T cell enrichment by negative magnetic selection using Easysep magnetic separation kits (STEMCELL Technologies). Cell suspension was stained with anti-CD4 (RPA-T4) ...
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bioRxiv - Cancer Biology 2020Quote: Aldefluor assay was performed to determine aldehyde dehydrogenase (ALDH) enzymatic activity using Aldefluor kit (Stem Cell Technologies, Catalog #01700) following manufacturer’s guidelines ...
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bioRxiv - Immunology 2021Quote: Human B cells were enriched from cryopreserved PBMCs using the EasySep™ Human B Cell Enrichment Kit (STEMCELL Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and resting CD4+ T cells (rCD4s) (EasySep™ Human Resting CD4+ T Cell Isolation Kit, Cat # 17962, StemCell Technologies) were isolated from HIV-1 aviremic donors living with HIV-1 ...
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bioRxiv - Immunology 2021Quote: ... Primary human B cells were isolated from PBMCs using the EasySep Human Naïve B cell isolation kit (Stemcell Technologies). Naïve B cells were cultured with or without VC (100 µg/mL ...
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bioRxiv - Immunology 2022Quote: ... Total CD4 T cells from PBMCs were isolated using the EasySep Human CD4+ T cell enrichment kit (StemCell technologies). Prior to infection ...
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bioRxiv - Immunology 2022Quote: ... CD8+ T cells were isolated from splenocytes of OT-I mice using a magnetic bead isolation kit (Stemcell Technology) and cultured following a previously published protocol (44) ...
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bioRxiv - Immunology 2022Quote: ... Memory CD4 T cells were isolated from PBMCs using the EasySep Memory CD4+ T cell enrichment kit (StemCell technologies). Total CD4 T cells from PBMCs were isolated using the EasySep Human CD4+ T cell enrichment kit (StemCell technologies) ...
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bioRxiv - Immunology 2022Quote: ... and CD8+ T cells were negative purified from cell suspension with untouched CD8+ T cell isolation kit (Stemcell Technologies). For the isolation of donor CD45.2+ OT-I T cells from C57BL/6 CD45.1+ recipient animals ...
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bioRxiv - Immunology 2022Quote: ... whereas the naïve B cells were isolated using the EasySep Human Naïve B cell isolation kit (Stem Cell Technologies). The purity of the B cells used in these experiments was > 95% ...
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bioRxiv - Molecular Biology 2022Quote: Myeloid cells were purified from PBMCs using Easy Sep Human Myeloid positive selection kit (Stem cell technology; Cat# 18653). The kit positively selects myeloid cells positive for CD33 and CD66b ...
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bioRxiv - Neuroscience 2023Quote: ... The Plating medium was consisted of 1 × BrainPhys Neuronal Medium/N2-A/SM1 kit (BrainPhys; Stem Cell Technologies, 05793) supplemented with 10 μM Y27632 ...
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bioRxiv - Microbiology 2023Quote: ... CD4+ T cells were enriched using the EasySep Human CD4+ T Cell Enrichment Kit (STEMCELL Technologies, Cat. No. 19052) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: Differentiation of iPSCs to hematopoietic progenitor cells (HPCs) was done using Stem Cell Technologies STEMdiff Hematopoietic Kit (StemCell Technologies). Upon reaching 80 % confluency ...
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bioRxiv - Immunology 2023Quote: ... and CD4+ cells were magnetically enriched using mouse negative selection CD4+ T cell isolation kits (STEMCELL Technologies and Biolegend). Live Tregs were sorted as fixable viability dyeneg (Thermo Fisher Scientific and Tonbo Biosciences) ...
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bioRxiv - Systems Biology 2023Quote: ... TRA-1-60) as well as differentiation into the three germ layers (STEMdiff Trilineage Differentiation kit, #05230, StemCell Technologies).
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bioRxiv - Immunology 2023Quote: CD4+ T cells were isolated from naïve splenocytes using the EasySep mouse CD4+ T cell enrichment kit (StemCell Technologies) and plated at 3x105 cells per well in a flat bottom 96 well plate ...
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bioRxiv - Cell Biology 2023Quote: ... CD4+ T cells were isolated from monocyte-depleted PBMCs using EasySepTM Human CD4+ Selection Kit II (StemCell Technologies Ltd.) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: NK Cells were enriched from freshly isolated PBMC using the EasySep™ Human NK Cell Isolation Kit (STEMCELL Technologies). NK cells were then counted and resuspended at 1M/mL in RPMI-1640 supplemented with 10% fetal bovine serum ...
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bioRxiv - Immunology 2023Quote: ... APCs from NOD spleens were obtained by depleting T cells using the Mouse CD90.2 Positive Selection Kit (STEMCELL Technologies) then pulsed with 15 µM of the indicated peptides and co-cultured with Tregs/Tconvs at a 1:1 ratio ...
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bioRxiv - Immunology 2023Quote: ... B cells were extracted by negative selection with the EasySep™ Human B Cell Isolation Kit (StemCell Technologies, #17954). EBV-containing supernatants from B95-8 cells were added to purified B cells supported by 30 µg mL-1 holo-transferrin and 2.5 µg mL-1 CpG (ODN2006) ...
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bioRxiv - Immunology 2023Quote: ... one piece (∼3g) was used for CD25+CD8- Treg enrichment using a custom thymic Treg selection kit (STEMCELL Technologies)102 ...