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Citations for Stemcell Technologies :
801 - 850 of 1527 citations for 3 Hydrazino 5 methyl 4H 1 2 4 triazol 4 ylamine hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... NPCs were plated at 1.25×5 cells/cm2 on Matrigel hESC-qualified Matrix-coated wells and maintained in Neural Progenitor medium (StemCell Technologies) with daily complete media change ...
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bioRxiv - Physiology 2022Quote: ... Cells were cultured in mTESR1 media at 37°C at 5% CO2 and passaged using accutase (Stemcell Technologies, Cat. # 07920) and media supplemented with Y-27632 2HCl (ROCK ...
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bioRxiv - Immunology 2024Quote: ... mice and bone marrow progenitors were plated on non- tissue treated dishes and differentiated into BMDM during a 6-day culture in complete Dulbecco’s modified Eagle’s medium (DMEM) containing 5% FBS and supplemented with 60 ng/ml recombinant murine M-CSF (Stemcell Technologies) in a water saturated atmosphere at 37°C ...
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bioRxiv - Immunology 2023Quote: ... The cells were incubated at 37°C and 5% v/v CO2 until confluency in PneumaCultTM-ExPlus media (STEMCELL Technologies) for 4-7 days before being switched to ALI culture conditions by removing the apical media and feeding the basal side with PneumaCultTM ALI medium (STEMCELL Technologies) ...
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bioRxiv - Cell Biology 2022Quote: ... GINS precursors were dissociated by 5-10 min TrypLE treatment and aggregated (typically 2.0-2.4 million cells/well) in AggreWell400 (STEMCELL Technologies, 34450) using the manufacturer’s recommended protocol ...
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bioRxiv - Genetics 2022Quote: ... HSPCs were plated at a concentration of 5×102 cells/mL in a methylcellulose-containing medium (GFH4435, Stem Cell Technologies) under conditions supporting erythroid and granulo-monocytic differentiation ...
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bioRxiv - Molecular Biology 2023Quote: ... and 5% O2 for 14 d before scoring colonies using the human mPB program on a STEMvision imager (StemCell Technologies).
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bioRxiv - Bioengineering 2023Quote: High cell density (4×107 cells/mL) hPSC strips containing 1.5mg/mL collagen with 5% Geltrex were collected in an Anti-Adherence Rinsing Solution (STEMCELL Technologies) treated 60mm Petri dish ...
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bioRxiv - Neuroscience 2024Quote: ... hiNS were supplied with a continuous perfusion of carbogen (95% O2/ 5% CO2) infused BrainPhys Imaging Optimized media (STEMCELL Technologies) and the following intracellular solution was used for microglial patch-clamp recordings ...
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bioRxiv - Neuroscience 2024Quote: ... Neural rosettes were isolated after 5 days in culture by selection using STEMdiff Neural Rosette Selection Reagent (STEMCell Technologies, 05832). The resulting NPCs were cultured on PLO and laminin-coated plates ...
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bioRxiv - Cell Biology 2024Quote: ... the monolayers were washed twice with PBS then incubated in dispase solution (5 units/mL in HBSS, Stemcell Technologies #07913) for 80 minutes at 37 °C to detach the monolayers from the plate ...
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bioRxiv - Cell Biology 2021Quote: Hepatostellate organoids at day 17 were treated with dissociation buffer, (1:1:1 mixture of 0.25% Trypsin-EDTA, Accutase (Stem cell Tech.), and Collagenase IV (1 mg/mL ...
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bioRxiv - Immunology 2022Quote: ... Remaining whole blood was diluted 1:1 with RPMI + 1% FBS + 2mM L-Glutamine + 100 U Penicillin/Streptomycin and layered onto SEPMATE tubes (STEMCELL Technologies) containing lymphoprep gradient (STEMCELL Technologies) ...
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bioRxiv - Developmental Biology 2022Quote: ... medium was replaced by 1 ml of a 1:1 mixture of UPC medium and reprogramming medium TeSR-E7 (STEMCELL Technologies). From day 4 to day 18-20 ...
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bioRxiv - Immunology 2021Quote: ... Remaining whole blood was diluted 1:1 with RPMI + 1% FBS + 2mM L-Glutamine + 100 U Penicillin/Streptomycin and layered onto SEPMATE tubes (STEMCELL Technologies) containing lymphoprep gradient (STEMCELL Technologies) ...
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bioRxiv - Immunology 2023Quote: ... Remaining whole blood was diluted 1:1 with RPMI + 1% FBS + 2mM L-Glutamine + 100 U Penicillin/Streptomycin (R1) and layered onto SEPMATE tubes (STEMCELL Technologies) containing lymphoprep gradient (STEMCELL Technologies) ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 ng/ml H-PDGF-BB (Shenandoah #100-16) and 2 μg/ml heparin (Stem Cell Technologies #07980). All cells were grown in a standard humidified cell culture incubator at 37 °C and 5% CO2 ...
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bioRxiv - Neuroscience 2020Quote: ... and further expanded in Neurocult NSA with proliferation supplement (StemCell Technologies; 10ng/ml rhFGF-2, 20ng/ml rhEGF) and incubated at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... PBMCs separation was performed within 2 h of blood withdrawal using SepMate™ tubes (StemCell Technologies, BC, Canada) as per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Viable BM cells (2×104) were seeded in 1.1 mL Mouse Methylcellulose Complete Media (M3434; Stem Cell Technologies) in uncoated 6 well plates (3471 ...
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bioRxiv - Bioengineering 2023Quote: ... T cells were cryopreserved at 2×107 cells per mL in CryoStor CS10 cryopreservation media (Stem Cell Technologies) until use ...
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bioRxiv - Immunology 2023Quote: ... was used at 2 µg/mL.11,14 Immunocult-ACF Human B Cell Expansion Supplement (BAC, Stem Cell Technologies) was used at 1:50 dilution per manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: ... rosette selection was performed by incubating for 2 hours with Neural Rosette Selection Reagent (Stem Cell Technologies, 05832), forcefully dislodging with warm DMEM/F12 ...
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bioRxiv - Microbiology 2023Quote: ... mixed 1:1 with PneumaCult™-Ex Plus Medium (STEMCELL Technologies cat# 16237380). All cell lines were discarded after passage 15 ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 1:10 dilution of 1 mg/ml DNase I (STEMCELL Technologies), triturated vigorously for 2 min ...
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bioRxiv - Immunology 2024Quote: ... (RPMI containing collagenase (250 U ml−1 of type 1 collagenase; STEMCELL technologies), DNase I (100 μg/mL ...
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bioRxiv - Cell Biology 2024Quote: ... The antibodies used are anti-TRA-1-60-AF488 (STEMCELL Technologies, 60064AD.1), anti-CXCR4-PE (BioLegend ...
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bioRxiv - Genetics 2024Quote: ... supplemented with 1:1000 with 1 mg/ml DNase I (StemCell Technologies, 07900)] warmed to 37 °C ...
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bioRxiv - Immunology 2020Quote: ... Remaining whole blood was diluted 1:1 with 1% RPMI (Table S4) and layered into a SEPMATE tube (STEMCELL Technologies, Cat#85450) pre-loaded with lymphoprep (Alere Technologies ...
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bioRxiv - Bioengineering 2024Quote: ... Dissociation was performed by incubation in dissociation solution (1:1 dilution of 2.5% Trypsin (GibcoTM, Cat#: 15090046) and 1 U/ml Dispase (Stemcell Technologies, Cat#: 15569185)) for 10 min under gentle shaking and occasional gentle pipetting at 28°C ...
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bioRxiv - Developmental Biology 2022Quote: ... the media was changed to pre-warmed 37°C mesoderm / Endothelial “priming” media (8 uM CHIR99021 +25 ng/ml BMP4, 1× Glutamax, 1× N2, 1× B27, Neurobasal, DMEM/F12, Stem Cell Technologies). One well of seeded cells was left untreated as negative control in StemFlex (data not shown) ...
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bioRxiv - Neuroscience 2021Quote: ... Suspension was mixed with 250 ul of 50% Iodixanol solution (25 mM KCl, 5 mM MgCl2, 10 mM Tris pH 8.0, 50% Iodixanol (60% stock from STEMCELL Technologies, 7820), 1x protease inhibitor (Roche ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were initially dissociated into single cell suspensions by accutase treatment (Stemcell Technologies, 07920, 30-60 min, 37 °C, 5% CO2) and then fixed and permeabilized using fixation/permeabilization buffers (Foxp3/Transcription Factor Staining Buffer Set ...
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bioRxiv - Immunology 2021Quote: Bone marrow colony forming assay was performed by plating 104 or 5×104 cells on methylcellulose (MethoCult M3434, Stem Cell Technologies). GEMM (Granulocyte/Erythrocyte/Macrophage/Megakaryocyte) ...
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bioRxiv - Immunology 2021Quote: ... and plated on Matrigel-coated plates at a density of 5 x 105 cells per cm2 in mTESR1 medium (StemCell Technologies) supplemented with 10 ng/mL of the RHO/ROCK pathway inhibitor ...
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bioRxiv - Developmental Biology 2021Quote: ... were plated at a density of ∼5 million neurons per cm2 in complete neuronal media recommended by the vendor as follows: BrainPhys Neuronal Medium (05790, STEMCELL Technologies) supplemented with iCell Neural Supplement B (M1029 ...
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bioRxiv - Neuroscience 2021Quote: ... 2.5 mL of cell suspension was transferred to a sterile 5 mL round-bottom flow cytometry tube and placed in the EasySep magnet (STEMCELL Technologies) for 5 min ...
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bioRxiv - Neuroscience 2021Quote: ... centrifuged at 200 x g for 5 min prior to be resuspended in STEMdiff™ Neural Progenitor Medium (Stem Cell Technologies) and seeded onto growth-factor-reduced Matrigel™-coated plates (8.6µg/cm2 ...
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bioRxiv - Neuroscience 2021Quote: ... with 10 μM Y-23632 (only for the initial 24 hours; Wako) and subcultured every 5–7 days using ReLeSR reagent (STEMCELL Technologies).
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bioRxiv - Cell Biology 2020Quote: ... Human pluripotent stem cell (hPSC)-derived cerebral organoids were grown at 37°C with 5% CO2 in STEMdiff Cerebral Organoid Kit cell culture media from STEMCELL Technologies Inc ...
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bioRxiv - Developmental Biology 2020Quote: ... The Geltrex was allowed to set for 40 minutes in a 37°C humidified incubator with 5% CO2 before being submerged in IntestiCult media (STEMCELL technologies).
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bioRxiv - Bioengineering 2021Quote: 5 wt% GelMAL-peptide and 5 wt% GelMA hydrogels containing 105 LSK/mL were immediately placed in 500 µL SFEM media (#09650, Stemcell Technologies) supplemented with 100 ng/mL SCF (#250-03 ...
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bioRxiv - Bioengineering 2021Quote: ... Cells (∼20 x 105 cells/cm2) were replated using the medium in which they were last cultured plus 5 μM ROCK inhibitor (#Y-27632, StemCell Technologies) and 5% Gibco™ KnockOut™ Serum Replacement (KSR ...
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bioRxiv - Cell Biology 2020Quote: ... The dissociated glands were spun down at 450 x g for 5 min and resuspended in ammonium chloride solution (STEMCELL Technologies) diluted (4:1 ...
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bioRxiv - Immunology 2020Quote: BMDNs were obtained by crushing long bones the bones in 5% FCS using a mortar and either isolated by negative selection using the EasySep Mouse Neutrophil Enrichment Kit (Stemcell Technologies) or by positive selection using biotinylated antibodies (see supplemental data for more details).
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bioRxiv - Cell Biology 2021Quote: ... Cells were cultured in a Cytomat 24 (Thermo Fischer Scientific) at 37°C and 5% CO2 in mTeSR1 medium with and without phenol red (STEMCELL Technologies), supplemented with 1% penicillin-streptomycin (Thermo Fischer Scientific).
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bioRxiv - Bioengineering 2020Quote: ... the hiPSCs were collected and seeded at 1.5 × 105 cells/cm2 cell density on glass coverslips with Rho-associated coiled-coil containing protein kinase (ROCK) inhibitor supplemented mTeSR media (5 μM, StemCell Technologies). The culture was maintained with daily media changes until 90% confluency was reached ...
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bioRxiv - Bioengineering 2020Quote: ... and cells were encapsulated in 5 mm diameter hydrogels (20 µL) and cultured for 7 days in 300 µL SFEM media (#09650 Stemcell Technologies) supplemented with 100 ng/mL SCF (#250-03 ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were washed with Dulbecco’s Modified Eagle Medium (DMEM/F12) and incubated for 70 min (37°C, 5 % CO2) in STEMdiff Neural Rosette Selection Reagent (StemCell Technologies). Rosettes were lifted by gently dispensing DMEM/F12 onto the colonies ...
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bioRxiv - Neuroscience 2024Quote: ... Plates were maintained in a 37°C/5% CO2 incubator and cells were fed every four days with neural maturation medium (BrainPhys plus SM1 supplement (Stemcell Technologies) supplemented with 10ng/ml of BDNF ...