Labshake search
Citations for Stemcell Technologies :
801 - 850 of 1437 citations for 1 2 Bromoethoxy 3 5 dimethylbenzene 97+% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... and maintained at 37C with 5%CO2 with daily removal of differentiated cells and replacement of mTeSR1 media (STEMCELL Technologies, Vancouver Canada). Cells were passaged routinely every 4 days using Dispase (STEMCELL Tec.) ...
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bioRxiv - Developmental Biology 2023Quote: ... Four-month-old cortical organoids were randomly selected and transferred to a recording chamber kept at 37L°C using a heating platform and a controller (TC-324C, Warner Instruments) in 5% methyl-cellulose in BrainPhys Imaging Optimized Medium (STEMCELL Technologies, #05796). Imaging was performed using a SP-8X microscope with a multiphoton laser ...
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bioRxiv - Immunology 2022Quote: Mouse HSCs were isolated by negative selection from 5-8 week old female Balb/C mouse bone marrow using EasySep™ mouse hematopoietic progenitor cell isolation kit (Stemcell Technologies) following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... we washed 80-90% confluent cultures with PBS-and dissociated cultures into a single-cell suspension with 5 min of Accutase (StemCell Technologies Inc.) treatment at 37°C ...
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bioRxiv - Bioengineering 2023Quote: ... Single cells were centrifuged at 200g for 5 minutes and resuspended in StemFlex supplemented with 10 µM Y-27632 (StemCell Technologies Inc.). We seeded 5.5×106 cells per 5.5 mL of mTeSR+ in each well of a six-well suspension plate ...
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bioRxiv - Cell Biology 2024Quote: ... treated monolayers were washed in PBS then incubated in 500 μL dispase (5 U/ml) in Hank’s balanced salt solution (Stemcell Technologies, Cat. #07913) at 37°C for 30 min ...
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bioRxiv - Cancer Biology 2024Quote: ... and collected in 5 mL of dissociation media consisting of Epicult-B Basal medium containing Mouse Epicult B supplement (Stemcell Technologies, 05610), 5% FBS ...
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bioRxiv - Cell Biology 2024Quote: ... on an automated cell-culture platform developed on a Hamilton Microlab STAR Liquid Handling System (Hamilton Company).2,43 Cells were maintained at 37 °C and 5% CO2 in mTeSR1 medium with and without phenol red (STEMCELL Technologies 85850, 05876), supplemented with 1% penicillin– streptomycin (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... Non-treated petri dishes of 100 mm diameter were coated with 5 mL of anti-adherence rinsing solution (STEMCELL Technologies, Vancouver, CA) for 5 minutes at 37°C to prevent cell adhesion ...
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bioRxiv - Synthetic Biology 2024Quote: WTC11 iPSCs90 were grown with 5% CO2 at 37°C cultured in mTeSR Plus Basal Medium (Stemcell technologies; Cat. No. 100-0276) on Greiner Cellstar plates (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: B cells from tonsils of 5 individuals were enriched using negative selection magnetic beads (EasySep Human B Cell Isolation kit, Stemcell technologies, #17954). They were then pooled to avoid batch effects ...
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bioRxiv - Genomics 2021Quote: ... onto Matrigel-coated (1 mg ml-1) 6-well plates 6 using ReLeSR (STEMCELL Technologies). Cells were differentiated to CD43+ hematopoietic progenitors using the StemCell Technologies Hematopoietic Kit (Cat #05310) ...
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bioRxiv - Developmental Biology 2021Quote: ... The cell pellet was resuspended into HF buffer (HBSS, 2% fetal bovine serum, 10mM Hepes pH 7.2) and NH4Cl (StemCell Technologies). The cell pellet was recovered after 5 min of centrifugation at 1000rpm and resuspended in organoid medium for organoid culture ...
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bioRxiv - Neuroscience 2020Quote: ... Transduced hiPSCs were selected and expanded in the presence of 2 μM puromycin and cryo-stored in mFreSR (STEMCELL Technologies) or further differentiated to OPCs as described below.
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bioRxiv - Neuroscience 2021Quote: ... the cells were counted and 2 million were cultured for 6 days in hematopoietic expansion media -Stemspan H3000(Stemcell Technologies), with the addition of EPO (R&D) ...
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bioRxiv - Neuroscience 2022Quote: ... For comparing to hCO cultured in STEMdiff™ Neural Organoid Basal Medium 2 (hCO + STEMdiff™ NOBM, STEMCELL Technologies, 08620), the remaining organoids in each well were imaged at day 12 on a REVOLVE microscope (ECHO ...
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bioRxiv - Neuroscience 2021Quote: ... Transfected cells were plated in 2 wells of a 6-well plate with mTeSR1 media supplemented with CloneR (STEMCELL Technologies) to enhance cell survival ...
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bioRxiv - Bioengineering 2023Quote: ... supplemented with 2 μl/mL ROCKi and seeded into microwells (6-well AggreWellTM 400 plates, Stem Cell Technologies, cat. 34460) at a density of 800–1,000 cells per microwell using the manufacturer’s recommended protocol ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were harvested and blocked with CD32 antibody (2 µl per 50,000 cells in 100 µL, STEMCELL Technologies Catalog #60012) for 15 minutes at 4C ...
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bioRxiv - Neuroscience 2023Quote: ... half of the medium was refreshed weekly with BrainPhys medium (BP) supplemented with 2% NeuroCult SM1 neuronal supplement (STEMCELL Technologies) and 1% penicillin/streptomycin.
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bioRxiv - Immunology 2024Quote: B cells subsets were obtained from WT or IL17−/− naïve mice or from mice 14 d after alloantigen immunization (2 × 107 mitomycin C–treated BALB/c splenocytes i.p.) by negative selection (EasySep; StemCell Technologies). Purity of the B cell subset was greater than 98% ...
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bioRxiv - Cell Biology 2024Quote: ALI cultures on the apical chamber of transwell inserts were infected with SARS-CoV-2 viral inoculum (MOI of 0.1; 100 µl/well) prepared in PneumaCult ALI (STEMCELL technologies) media ...
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bioRxiv - Cancer Biology 2024Quote: ... Every 2-4 weeks organoids were passaged by mechanical pipetting Matrigel gently using Dispase in DMEM/F12 media (STEMCELL Technologies #) and several washes with PBS until Matrigel was cleared out ...
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bioRxiv - Developmental Biology 2024Quote: Placenta cell suspensions were suspended in Iscove’s MDM with 2% fetal bovine serum and cultured using a MethoCult GF M3434 Optimum kit (STEMCELL Technologies). 10,000 cells from each sample were plated in 35-mm culture dishes (STEMCELL Technologies ...
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bioRxiv - Molecular Biology 2020Quote: ... or β-thalassemia patients were cultured for 14-16d at 37°C and 5% CO2 in SFEM II medium (STEMCELL Technologies, Vancouver, Canada) as previously described37,38 ...
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bioRxiv - Physiology 2020Quote: ... and maintained at 37 °C with 5% CO2 with daily removal of differentiated cells and replacement of mTeSR1 media (STEMCELL Technologies, Vancouver, Canada). Cells were passaged routinely every 4 days using Dispase (STEMCELL Technologies) ...
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bioRxiv - Cancer Biology 2020Quote: Primary mouse clonogenic progenitor assays or colony forming units (CFU) assays were performed by plating 5×105 unfractionated nucleated BM cells in methylcellulose M3434 (StemCell Technologies, Vancouver, Canada) in 1 ml duplicates ...
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bioRxiv - Molecular Biology 2023Quote: ... 2.5% and 5% (v/v) of the hydrophilic concentrate BMGLS in PneumaCult™ ExPlus or PneumaCult™ ALI medium (Stemcell Technologies, Vancouver, Canada) supplemented with MycoZAP (Lonza ...
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bioRxiv - Molecular Biology 2024Quote: ... 5% CO2 and 94% N2 for 7 minutes at a rate of 20 L/min using Single Flow Meter (STEMCELL Technologies, Cat. #27311). The sealed chamber was then transferred to 37°C and cells incubated for 16 hours before harvesting for further analysis.
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bioRxiv - Molecular Biology 2023Quote: ... Medium was replaced every two days and cells were split every 5 to 6 days using Gentle Dissociation reagent (STEMCELL Technologies, Vancouver, Canada). hES cells were routinely tested for Mycoplasma using the Mycoplasma PCR Detection kit (Applied Biological Materials ...
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Autophagy suppression in DNA damaged cells occurs through a newly identified p53-proteasome-LC3 axisbioRxiv - Cell Biology 2024Quote: Mouse Intestinal Organoids were incubated in 24-well cell culture plates at 37 °C under 5% CO2 in IntestiCult Complete Organoid Medium (#06005, Stem Cell Technologies, Canada) containing CHIR-99021 (3 μM final concentration ...
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bioRxiv - Cancer Biology 2024Quote: ... NK cells were stained with CellTrackerTM Green CMFDA (5-chloromethylfluorescein diacetate) (InvitrogenTM C7025) diluted to 10 µM in serum free ImmunoCultTM NK Cell Base Medium (STEMCELL Technologies 100-0712) for 30 min ...
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bioRxiv - Synthetic Biology 2024Quote: ... HSPCs derived from healthy patients or iPSC-derived HPCs were cultured for 14d at 37°C and 5% CO2 in SFEMII medium (STEMCELL Technologies, Vancouver, Canada) as previously described19,20 ...
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bioRxiv - Immunology 2024Quote: ... the pooled blood was carefully layered over 5 mL of Ficoll-Paque PREMIUM 1.084 (Cytiva, Uppsala, Sweden) in 15 mL SepMate-50 tubes (STEM Cell Technology, Cambridge, MA). PBMCs were collected from the interphase layer after centrifugation at 1200xg for 30 min.
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bioRxiv - Bioengineering 2024Quote: ... These cells were maintained in a 5% CO2 incubator at 37°C and were fed every 24 to 48 hours with mTeSR Plus medium (StemCell Technologies, 100-0276). The iPSCs were passaged using the StemPro EZPassage tool (Life Technologies ...
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bioRxiv - Synthetic Biology 2024Quote: ... 10,000 cells were plated per 12-well plate well onto 5% biolaminin 521 LN and differentiation started 6 hrs post plating in TeSR-E6 (Stem Cell Technologies, 05946) with CHIR99021 (Tocris ...
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bioRxiv - Genomics 2021Quote: ... at a 1:100 dilution and in mTeSR™1 Medium (85851, STEMCELL Technologies, Vancouver, Canada) supplemented with 1% Penicillin/Streptomycin (30-002-CI ...
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bioRxiv - Immunology 2021Quote: ... medium was switched to 1:1 of MTEC and PneumaCult-Ex PLUS medium (StemCell Technologies, #05040) and fed every other day until expansion of cells to ∼80% confluent ...
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Pluripotent stem cell SOX9 and INS reporters facilitate differentiation into insulin-producing cellsbioRxiv - Developmental Biology 2021Quote: ... 1 µM XXI (Stem Cell Technologies) or 1 µM LY411575 (Selleck Chemicals) ...
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bioRxiv - Immunology 2021Quote: ... containing 1:100 SM1 (StemCell Technologies), 1:100 NEAA (Gibco) ...
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bioRxiv - Cell Biology 2021Quote: ... α-GFAP (Stemcell Technologies 60048; 1:100), α-IBA-1 (Santa Cruz Biotechnology sc-32725 ...
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bioRxiv - Cell Biology 2021Quote: ... mTeSR™1 (StemCell Technologies, 05850) media supplemented with penicillin/streptomycin (Gibco ...
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bioRxiv - Neuroscience 2020Quote: ... RI (1 μm; Stem Cell Technology) and LiCl (2 mM ...
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bioRxiv - Cancer Biology 2022Quote: ... Ionomycin (1 μg/ml, STEMCELL technologies), and monensin (2 μM ...
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bioRxiv - Cell Biology 2022Quote: ... in mTeSR-1 media (StemCell Technologies) using either gentle cell dissociation reagent (GCDR ...
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bioRxiv - Neuroscience 2021Quote: ... to mTeSR 1 (StemCell Technologies 85850), and total DNA amounts of 5μg (2.5μg donor vector plus 1.25μg of each TALENS arm plasmid ...
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bioRxiv - Pathology 2022Quote: ... 1 IU/mL Heparin (StemCell Technologies), 100 nM LDN193189 (StemCell Technologies) ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 mM SB431542 (Stem Cell Technologies), 0.8 mM VPA (Sigma-Aldrich ...
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bioRxiv - Immunology 2020Quote: ... in mTeSR™1 (STEMCELL Technologies) medium ...
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bioRxiv - Immunology 2021Quote: ... and 1 µM SR1 (StemCell Technologies). Cells were cultured at 37°C ...