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Citations for Stemcell Technologies :
751 - 800 of 980 citations for Recombinant Human PRMT5 Flag & His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... CD4+ T cells were negatively isolated using the RosetteSep™ Human CD4+ T Cell Enrichment Cocktail (Stem Cell Technologies # 15061) or the EasySepTM Human Naïve CD4+ T cell Isolation Kit (Stem Cell Technologies #17953 ...
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bioRxiv - Immunology 2023Quote: ... T cells are isolated using an EasySep™ Human T Cell Enrichment Kit as described by the manufacturer (Stemcell Technologies). For T cell activation ...
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bioRxiv - Bioengineering 2023Quote: Primary human CD3+ T cells from 3 unique donors were isolated by negative selection (T Cell Enrichment Cocktail, Stemcell Technologies) from peripheral blood and were grown in RPMI + 10% FBS + 1% PS supplemented with 50 U/mL of IL2 ...
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bioRxiv - Cell Biology 2023Quote: ... Primary NK cells were isolated from peripheral blood by negative selection using RosetteSep human NK enrichment cocktail (Stemcell Technologies, 15065). Blood was layered onto a Ficoll-Paque (Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... peripheral blood mononuclear cells (PBMCs) were isolated from human donor leukopaks by gradient centrifugation on lymphoprep (cat#07861, Stemcell Technologies). Thereafter ...
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bioRxiv - Molecular Biology 2023Quote: ... and 5% O2 for 14 d before scoring colonies using the human mPB program on a STEMvision imager (StemCell Technologies).
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bioRxiv - Microbiology 2023Quote: ... Monocytes were isolated from PBMCs using EasySep Human monocyte enrichment kit without CD16 depletion (StemCell Technologies, Vancouver, British Columbia, Canada) according to the manufacturers’ instructions ...
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bioRxiv - Cancer Biology 2024Quote: Human neural progenitor cells (hNPCs) were generated by a single-cell monolayer protocol using STEMDiff Neural Induction Medium (StemCell Technologies) as described previously 19 ...
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bioRxiv - Cancer Biology 2024Quote: Freshly isolated human HSPCs were plated at 200,000 cells/ml and cultured in IMDM medium supplemented with 25% BIT9500 (StemCell Technologies), 1% penicillin/streptomycin (P/S) ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were either left unactivated or activated with Immunocult Human CD3/CD28/CD2 T cell activator per manufacturer’s instructions (StemCell Technologies) for 24 or 48hrs ...
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bioRxiv - Cell Biology 2019Quote: ... Primary CD4+ T cells were purified from the blood of healthy donors by negative selection using the Human CD4+ T Cell Enrichment cocktail (StemCell Technologies). These primary cells were cultured in complete RPMI 1640 medium.
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bioRxiv - Cell Biology 2020Quote: ... The buffy coat was first separated by Ficoll-Paque Plus solution and the NK cells were then purified from the Peripheral Blood Mononuclear Cells (PBMCs) by negative selection using the EasySep Human NK Cell Enrichment Kit (STEMCELL Technologies), according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... peripheral blood monocytes (PBMCs) were isolated from the whole blood of healthy donors using an EasySep Direct Human PBMC Isolation Kit (StemCell Technologies) through negative selection ...
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bioRxiv - Neuroscience 2021Quote: ... integrated and inducible pure human cortical-like glutamatergic neurons (i3Ns) was completed in two weeks in BrainPhys media (STEMCELL Technologies, 05790) (Figure ...
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bioRxiv - Developmental Biology 2019Quote: Sorted cell populations were counted and plated at 5000 cells into 2 ml of methylcellulose medium (Human enriched H4435, Stemcell Technologies). Cells were incubated in the assay for 14 days and then scored.
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bioRxiv - Microbiology 2019Quote: ... monocytes were isolated from thawed PBMCs using the EasySep Human Monocyte Enrichment Kit without CD16 Depletion (STEMCELL Technologies, Cat. No. 19058). The purified monocytes were seeded into a 24-well tissue culture treated plate at ~1.2 million cells/well in Advanced RPMI medium (Gibco ...
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bioRxiv - Developmental Biology 2019Quote: Human iPSC line WTC11 (a kind gift from Bruce Conklin, Gladstone Institute of Cardiovascular Disease) was maintained in mTeSR1 (Stemcell Technologies) on Matrigel (Corning ...
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bioRxiv - Immunology 2021Quote: ... CD8+ T cells were isolated from peripheral blood of donor 2395 via negative selection using the RosetteSep Human CD8+ T Cell Enrichment Cocktail (StemCell Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Huh7 and HepG2 and autologous CD8+ T cells (pre-activated with ImmunoCult™ Human CD3/CD28 T Cell Activator, Stemcell Technologies, Canada according to the manufacturer’s instruction) ...
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bioRxiv - Immunology 2021Quote: ... Primary CD4+ or CD8+ T cells were isolated using EasySep Human CD4+ or CD8+ T Cell Enrichment Kits (Stem Cell Technologies) and cultured in RPMI 1640 (ATCC ...
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bioRxiv - Synthetic Biology 2021Quote: ... Primary human CD4+ T cells were isolated from anonymous donor blood after apheresis by negative selection (STEMCELL Technologies #15062 and 15023). T cells were cryopreserved in RPMI-1640 (Corning #10-040-CV ...
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bioRxiv - Immunology 2021Quote: ... cells were preactivated for 5 days with IL-2 and either 1:1 aAPCs or 25 μl/ml CD3/CD28/CD2 tetramer (ImmunoCult Human CD3/CD28/CD2 T Cell Activator, STEMCELL Technologies). L cells (ATCC CRL-2648 ...
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bioRxiv - Bioengineering 2021Quote: ... CD4+ T cells were isolated from PBMCs by negative magnetic selection using EasySep Human CD4 T cell Isolation Kit (StemCell Technologies). Isolated T cells were then plated in ImmunoCult-XF T Cell Expansion Medium (StemCell Technologies ...
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bioRxiv - Bioengineering 2020Quote: HSPC (CD34+) were isolated from cord blood using the EasySep™ Human Cord Blood CD34 Positive Selection Kit II (StemCell Technologies) according to the manufacture’s guidelines ...
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bioRxiv - Microbiology 2020Quote: ... purified human B cells were obtained from the Human Immunology Core of the University of Pennsylvania under an Institutional Review Board-approved protocol and were isolated using the RosetteSep Human B Cell Enrichment Cocktail (StemCell Technologies) as per protocol ...
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bioRxiv - Genomics 2021Quote: ... Cord blood MNCs (4) were received frozen and then CD34+ selected using the EasySep human whole blood CD34 positive selection kit (Stemcell Technologies) as per the manufacturer’s instructions ...
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Hydrogen sulfide blocks HIV rebound by maintaining mitochondrial bioenergetics and redox homeostasisbioRxiv - Microbiology 2021Quote: ... The CD4+ T cells were purified from 50 × 106 PBMCs using EasySep™Human CD4+ T cell isolation kit (STEMCELL Technologies). Primary CD4+ T cells were cultured at 37°C in a 5 % (v/v ...
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bioRxiv - Immunology 2021Quote: Naïve IgM+IgD+B cells were purified by negative selection using the EasySepTM human naive B cell enrichment kit (19254; StemCell Technologies) from healthy subject PBMCs ...
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bioRxiv - Microbiology 2021Quote: ... buffy coats (from anonymous healthy blood donors from Mississippi Blood Center) were separated by Ficoll and CD4+ T-cells purified using RosetteSep Human CD4+ T-cell enrichment kit (STEMCELL Technologies). CD4+ T-cells cells were maintained in Roswell Park Memorial Institute 1640 medium (RPMI ...
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Naked Mole-Rat Hematopoietic Stem and Progenitors are Highly Quiescent with an Inherent Myeloid BiasbioRxiv - Cell Biology 2021Quote: ... between 50-250*103 cells per 200µl of the following culture media: Human LT-HSCs (LIN−/CD38lo/CD34+/CD45RA−/CD90+) in StemSpan Serum-free expansion medium (SFEM; Stemcell Technologies) supplemented with 100ng/ml human SCF ...
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bioRxiv - Microbiology 2022Quote: ... HNAE cells were collected from healthy donors (human ethics approval GLY/01/15/HREC) and expanded using Pneumacult Ex+ (Stemcell technologies). HNAE cells were differentiated at air-liquid interface in 6.5 mm transwell with a 0.4μm polyester 156 membrane (Corning ...
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bioRxiv - Microbiology 2021Quote: ... total CD4+ T cells were isolated from PBMCs by negative magnetic separation using EasySep Human CD4+ T cell isolation kit (StemCell Technology). Following treatment for 5 days (Fig ...
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bioRxiv - Cell Biology 2020Quote: ... 70–90% confluent human iPSC colonies were passaged chemically (Versene, Life Technologies, Cat. 15040-066 or ReLeSR, StemCell Technologies, Cat. 05872) or mechanically by StemPro® EZPassage™ Disposable Stem Cell Passaging Tool (Life Technologies ...
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bioRxiv - Immunology 2021Quote: Primary human CD4+ T cells were isolated from PBMCs using a Human resting CD4+ T Cells Isolation Kit I according to the manufacturer instructions (STEMCELL Technologies). Briefly ...
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bioRxiv - Immunology 2019Quote: ... cryopreserved PBMCs were thawed (as above) and CD4 T cells were isolated by negative selection using the EasySep Human CD4 Enrichment Kit (Stemcell Technologies) before DNA extraction with the QiaAMP Blood Mini Kit (Qiagen) ...
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bioRxiv - Immunology 2019Quote: ... Peripheral blood mononuclear cells from whole blood freshly drawn from healthy human volunteers were isolated via centrifugation using RosetteSep and Lymphoprep reagents (StemCell Technologies). Monocytes were separated by adherence to tissue culture flasks ...
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bioRxiv - Synthetic Biology 2020Quote: ... Regulatory T cells were isolated using the EasySep human CD4+CD127lowCD25+ regulatory T cell isolation kit according to manufacturer’s protocol (STEMCELL Technologies, 18063). Primary T cells were cultured in X-Vivo 15 media (Lonza ...
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bioRxiv - Immunology 2019Quote: ... naïve CD44+ T cells were enriched by magnetic separation using the EasySep™ Human Naïve CD4+ T Cell Isolation Kit (Stemcell Technologies). 55×104 T cells were cultured for 6 days in the presence of plate-bound αCD3ε (5 μg mL−1) ...
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bioRxiv - Immunology 2021Quote: ... CD45+ cells were depleted from the same patient sample to obtain CD45-cells for co-culture using the EasySep Human CD45 Depletion Kit from StemCell Technologies Cat# 17898 ...
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bioRxiv - Immunology 2020Quote: ... Resting CD4+ T cells (rCD4s) were isolated from PBMC by magnetic negative selection using the EasySep Human Resting CD4+ T cell Isolation Kit (StemCell Technologies). In some experiments ...
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bioRxiv - Evolutionary Biology 2020Quote: Cerebral organoids with telencephalic identity from human and ape cells were generated as previously (Lancaster et al. 2017) with the following modification: Aggrewell 800 plates (StemCell Technologies) containing 300 microwells ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and CD4+ T-cells were isolated via the Easy-Sep™ Rosette Human CD4+ T-cell enrichment kit (Stemcell Technologies, Canada) according to manufacturer’s instructions and purity of isolated CD4+ T-cells was controlled on a FACSverse™ flow cytometer (BD Biosciences ...
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bioRxiv - Genomics 2021Quote: ... CD8+ T cells were purified from thawed PBMCs by negative selection using EasySep Human CD8+ T Cell Enrichment Kit (Cat#19053, STEMCELL Technologies) according to the manufacturer’s protocol.
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bioRxiv - Genomics 2021Quote: ... B cells were purified from thawed PBMCs by negative selection using EasySep Human B Cell Enrichment Kit (Cat#19844, STEMCELL Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... Total CD8+ T cells were isolated from unfractionated PBMCs using the EasySep Human CD8+ T Cell Isolation Kit (Stem Cell Technologies) with a DynaMag-2 magnet (ThermoFisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... Naïve CD4+ T-cells (CD3+CD4+CD45RA+CD45RO-) were purified from PBMC using the EasySep Human CD4+ T-cell Isolation Kit (Stemcell Technologies) to >95% purity according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... The negative fraction remaining after the positive selection of CD14+ cells was used for pan-DC enrichment employing the EasySep human pan-DC enrichment kit (StemCell Technologies). Total pan-DC were resuspended with 20,000 CD14+ cells and sent for sequencing ...
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bioRxiv - Microbiology 2020Quote: ... CD4+ T cells were enriched from blood of healthy donors using RosetteSep Human CD4+ T cell enrichment cocktail (Stemcell Technologies, Canada) according to the manufacturer’s instructions followed by Ficoll density gradient centrifugation ...
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bioRxiv - Immunology 2021Quote: ... 100μL of Isolation Cocktail and 100μL of Rapid Spheres (Easy Sep™ Direct Human Total Lymphocytes Isolation Kit: 19655, StemCell Technologies) were added to the remaining whole blood ...
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bioRxiv - Immunology 2021Quote: ... plasmablasts were enriched from the whole blood (day 7 after second vaccination) by negative selection using custom direct human plasmablasts isolation kit containing paramagnetic beads and antibodies for negative selection (StemCell Technologies). Enriched cells were stained 30 min on ice in a RoboSep buffer (StemCell Technologies ...