Labshake search
Citations for Stemcell Technologies :
751 - 800 of 1004 citations for L Phenylalanine N T Boc 2 13C 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2020Quote: ... were depleted that CD4+ T cells were negative selection and CD25+ cells were positive selection using the Easy Sep™ Human CD4+CD25+ T Cell Isolation Kit (StemCell Technologies, Vancouver, Canada). We confirmed the removal of about 90 % of T-regs by FCM (S5 ...
-
bioRxiv - Immunology 2020Quote: ... Each antibody clone and lot was titrated to optimal staining concentrations using primary murine samples with all appropriate positive and negative controls: polyclonal murine CD8 T cells purified by positive selection kit (Stem Cell Technologies, Vancouver, Canada) stimulated with PMA/Ionomycin via eBioscience Cell Stimulation Cocktail (ThermoFisher Scientific ...
-
bioRxiv - Immunology 2022Quote: ... T cells from were then activated with Miltenyi MACS GMP T cell TransAct™ CD3/CD28 beads and seeded 1×106 T cells/ml in serum-free StemCell Immunocult™-XF media (Cat#10981, StemCell Technologies, Vancouver, Canada) with 20U/ml clinical grade human IL-2 (Novartis) ...
-
bioRxiv - Cancer Biology 2023Quote: An Easy Step Human CD8+ T Cell Isolation Kit was used to isolate CD8+ T cells from a donor PBMC sample via negative selection (Cat #, 17913, Stem Cell Technologies, Vancouver, Canada).
-
bioRxiv - Genomics 2022Quote: CD4 T-cells were isolated from 10-20 ml of peripheral blood using EasySep Direct Human CD4+ T cell Isolation kit (Stem Cell Technologies, cat #19662). The isolated CD4 T-cells were stored in Crystor CS10 (Stem Cell Technologies ...
-
bioRxiv - Immunology 2023Quote: ... CD4 T cells were isolated from the spleen and LNs of indicated naïve OT-II strains with Mouse CD4 T Cell Isolation Kit (STEMCELL Technologies 19852 or BioLegend 480033) and labeled with 1.0 µM CFSE (Thermo Fisher ...
-
bioRxiv - Immunology 2024Quote: ... Viable T cells were enriched using the Easy Sep T cell isolation Kit followed by the Easy Sep Dead Cell removal Kit (Stem Cell Technologies, Vancouver, BC).
-
bioRxiv - Neuroscience 2023Quote: Flow cytometric analysis was performed on healthy control PBMCs after 48h stimulation with ImmunoCult™ human CD3/CD28/CD2 T cell activator tetrameric antibody complex (Stemcell Technologies cat. 10970) at 20μl/ml ...
-
bioRxiv - Bioengineering 2024Quote: ... Human peripheral blood mononuclear cells (PBMCs) were sourced from OriBiotech (Shanghai, China) and cultured in ImmunoCult™-XF T Cell Expansion Medium (StemCell Technologies, Vancouver, Canada). Mouse PBMCs were purified from mouse whole blood by Mouse PBMC Isolation Kit (Solarbio ...
-
bioRxiv - Biophysics 2024Quote: ... hiPSCs from N=1 donor were differentiated to definitive endoderm by using the STEMdiff™ Definitive Endoderm Kit (STEMCELL Tech., Vancouver, BC, Canada). Subsequently ...
-
bioRxiv - Neuroscience 2021Quote: ... placed in a stereotaxic frame and injected with either 100,000 hNSCs per side (2 μl/injection) or vehicle (2 μl HBSS with 20 ng/ml epidermal growth factor [STEMCELL Technologies, #78003] and human fibroblast growth factor [STEMCELL ...
-
IRF1 regulates self-renewal and stress-responsiveness to support hematopoietic stem cell maintenancebioRxiv - Cell Biology 2023Quote: ... Briefly, PB was collected in FACS buffer (PBS, 2 % FBS, 2 mM EDTA) supplemented with 0.0004 % heparin solution (STEMCELL Technologies Inc.). RBCs was removed by incubation at 37 °C for 25 minutes with 1 % Dextran solution in PBS ...
-
bioRxiv - Neuroscience 2024Quote: ... Cells were centrifuged at 200 x g for 2 min and resuspended in neural media (NM; Supplementary Table 2) supplemented with 0.02 µg/mL FGF2 (StemCell Technologies, #78003) and 0.02 µg/mL EGF (StemCell Technologies ...
-
bioRxiv - Immunology 2021Quote: ... T cells from were then activated with Miltenyi MACS GMP T cell TransAct™ CD3/CD28 beads and seeded 1×10^6 T cells/ml in serum-free StemCell Immunocult™-XF media (StemCell Technologies, Vancouver, Canada) with clinical grade 20U/ml human IL-2 (Novartis)
-
bioRxiv - Microbiology 2024Quote: ... Bulk CD4+ T cells were subsequently isolated from PBMCs by magnetic negative selection using an EasySep Human CD4+ T cell isolation kit (STEMCELL Technologies; per the manufacturer’s instructions). Isolated CD4+ T cells were suspended in RPMI 1640 (Sigma-Aldrich ...
-
bioRxiv - Neuroscience 2020Quote: ... Medium was refreshed every 2-3 days and cells were passaged 1-2 times per week using an enzyme-free reagent (ReLeSR, Stem Cell Technologies). For autophagy induction cells were treated with 200 µM Rapamycin (ChemCruz ...
-
bioRxiv - Neuroscience 2024Quote: ... Embryoid body media (EBM) was used for days 2 to 11 (Supplementary Table 2) and supplemented with 10 µM SB431542 (Stemcell Technologies, #72234), 0.1 µM LDN193189 (MedChemExpress ...
-
bioRxiv - Molecular Biology 2021Quote: ... and human recombinant IL-2 (Stemcell Technologies, Vancouver, Canada). Activated CD4+ T cells were transfected with pVax-CasRx and gRNA plasmids by nucleofection using Lonza 4d nucleofector ...
-
bioRxiv - Cell Biology 2019Quote: ... in 2 ml of SFEM II (Stem Cell Technologies) supplemented with 10 ng ml−1 IL3 ...
-
bioRxiv - Bioengineering 2022Quote: ... supplemented with 2 mg/mL heparin (#07980; STEMCELL Technologies) on 10 μg/mL human plasma fibronectin (#FC010 ...
-
bioRxiv - Cancer Biology 2022Quote: ... Fibroblast Growth Factor-2 (FGF2, StemCell Technologies Cat#78134.1), Vascular Endothelial Growth Factor-A (VEGF-165 ...
-
bioRxiv - Cancer Biology 2020Quote: ... supplemented with 2 µg/ml heparin sulfate (STEMCELL Technologies) or SFM supplemented with 20 ng/ml human recombinant epidermal growth factor (hEGF ...
-
bioRxiv - Cancer Biology 2021Quote: ... and heparin sulfate (2 µg/mL; 07980, STEMCELL Technologies). The plates were kept in a 37°C ...
-
bioRxiv - Developmental Biology 2021Quote: ... 2 mg/ml DNase I (Stem Cell Technologies, 07900)) ...
-
bioRxiv - Cancer Biology 2023Quote: ... and 2 μg/ml heparin (#07980, Stem Cell Technologies). All cells were tested for mycoplasma contamination ...
-
bioRxiv - Bioengineering 2023Quote: ... IMDM with 2% FBS (StemCell Technologies, Vancouver, BC, Canada). This HSC:MSC resuspension was added to 1 mL MethoCult H4034 Otimum (StemCell Technologies ...
-
bioRxiv - Cancer Biology 2022Quote: ... heparin (2 μg/ml, Stem Cell Technologies, cat# 7980), human bFGF (10 ng/ml ...
-
bioRxiv - Cancer Biology 2022Quote: ... human recombinant EGF (2 ng/mL, STEMCELL Technologies, #78006.2), antibiotic-antimycotic (1X ...
-
bioRxiv - Immunology 2023Quote: ... An underlay of 2 ml of lymphoprep (STEMCELL technologies) was added ...
-
bioRxiv - Neuroscience 2024Quote: ... 2% NeuroCult™ SM1 (Stemcell technologies, cat. no. 05711), 1% N-2 Supplement (Thermo Scientific ...
-
bioRxiv - Biophysics 2019Quote: ... Cells were washed and resuspended in phenol red-free Neurobasal Medium (Lifetech) supplemented with L-glutamine (Stem Cell Technologies) and SM1 (NeuroCult ...
-
bioRxiv - Immunology 2022Quote: ... Infectious units per million were estimated by limiting dilution statistics using L-Calc™ Limiting Dilution Software (STEMCELL Technologies).
-
bioRxiv - Microbiology 2023Quote: ... and Heparin solution (2 μl/ml; Stem cell technologies #07980).
-
bioRxiv - Cell Biology 2023Quote: ... supplemented with 2 µM Thiazovivin (StemCell Technologies, Cambridge, MA 02142) and plated in a Matrigel-coated 12-well plate at a density of 3 x 105 cells per well ...
-
bioRxiv - Cancer Biology 2023Quote: ... heparin solution (final concentration, 2 µg/mL, STEMCELL Technologies, 07980), recombinant human EGF (20 ng/mL ...
-
bioRxiv - Cell Biology 2023Quote: ... for EB formation in APEL-2 media (Stemcell Technologies, 5275) containing 10µM ROCK inhibitor ...
-
bioRxiv - Bioengineering 2024Quote: ... and 2 mg/mL dispase II (STEMCELL Technologies, Vancouver, Canada) for 4 hours under gentle agitation at 37° C ...
-
bioRxiv - Biophysics 2024Quote: ... ovaries were treated with collagenase (2 mg/mL; StemCell Technologies) and incubated in Modified Bath Saline (MBS ...
-
bioRxiv - Cancer Biology 2022Quote: ... MDS-L cell line was cultured in the presence of 10 ng/mL human recombinant IL-3 (Stem Cell Technologies). The MDS/AML patient samples used in this study were obtained with written informed consent under approval by the IRBs of the Albert Einstein College of Medicine ...
-
bioRxiv - Cancer Biology 2024Quote: ... The frequency of LSCs was calculated using Poisson statistics with the L-Calc Software for limiting dilution analysis (StemCell Technologies)
-
bioRxiv - Biochemistry 2022Quote: ... NPC cells were cultured on a poly-l-ornithine- and laminin-coated six-well plate (Falcon) at 37°C in neural expansion media (STEMCELL Technologies, 70% DMEM ...
-
bioRxiv - Immunology 2022Quote: ... Remaining whole blood was diluted 1:1 with RPMI + 1% FBS + 2mM L-Glutamine + 100 U Penicillin/Streptomycin and layered onto SEPMATE tubes (STEMCELL Technologies) containing lymphoprep gradient (STEMCELL Technologies) ...
-
bioRxiv - Developmental Biology 2021Quote: ... The progenitor frequency was calculated using the method of maximum likelihood applied to Poisson distribution with L-Calc software (StemCell Technologies). OP9s were purchased from ATCC ...
-
bioRxiv - Immunology 2021Quote: ... Remaining whole blood was diluted 1:1 with RPMI + 1% FBS + 2mM L-Glutamine + 100 U Penicillin/Streptomycin and layered onto SEPMATE tubes (STEMCELL Technologies) containing lymphoprep gradient (STEMCELL Technologies) ...
-
bioRxiv - Immunology 2021Quote: ... 100μL of Isolation Cocktail and 100μL of Rapid Spheres (Easy Sep™ Direct Human Total Lymphocytes Isolation Kit: 19655, StemCell Technologies) were added to the remaining whole blood ...
-
bioRxiv - Cell Biology 2023Quote: ... and sub-plated at 25 x 104 cells/ml on dishes coated with 50 μg/ml poly-L-ornithine in BrainPhys neuronal medium (STEMCELL Technologies) containing N2 (Thermo Fisher Scientific) ...
-
bioRxiv - Neuroscience 2023Quote: ... Neural progenitor cells were then dissociated and replated on poly-l-ornithine/fibronectin/laminin-coated plates and maintained in BrainPhys Basal medium (StemCell Technologies) containing B-27 supplement ...
-
bioRxiv - Immunology 2023Quote: ... Remaining whole blood was diluted 1:1 with RPMI + 1% FBS + 2mM L-Glutamine + 100 U Penicillin/Streptomycin (R1) and layered onto SEPMATE tubes (STEMCELL Technologies) containing lymphoprep gradient (STEMCELL Technologies) ...
-
bioRxiv - Cancer Biology 2020Quote: ... Pellet is resuspended in 2-5ml accutase™ (StemCell Technologies, 07920), depending on the sample amount ...
-
bioRxiv - Developmental Biology 2022Quote: ... cells were collected with 2 mL mTeSRplus (Stem Cell Technologies, 05825), span down and resuspended in 1 mL mTeSRplus ...