Labshake search
Citations for Stemcell Technologies :
751 - 800 of 1392 citations for Anti ChAT SAP Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... starting from freshly isolated PBMCs from 69 PLWH using the EasySep CD4 T cell isolation kit (Stemcell Technologies). CD4+ T cells were frozen as dry pellet at −80°C prior to DNA extraction with the DNeasy Blood & Tissue kit (Qiagen) ...
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ALS iPSC-derived microglia and motor neurons respond to astrocyte-targeted IL-10 and CCL2 modulationbioRxiv - Neuroscience 2023Quote: ... iPSCs were differentiated into hematopoietic progenitor cells (HPCs) using the STEMdiff Hematopoietic Kit (StemCell Technologies, Vancouver, BC, Canada). Floating HPCs were collected and plated at 50,000 cells/mL in STEMdiff microglia differentiation media (StemCell Technologies ...
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bioRxiv - Immunology 2023Quote: ... CD4+ T cells were isolated from PBMCs with the EasySep Human CD4+ T cell Enrichment Kit (STEMCELL Technologies). Genomic DNA was isolated from a median of 3.65 million CD4+ T cells (IQR 2.48-4.98 ...
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bioRxiv - Bioengineering 2023Quote: ... then maintained in culture using reagents and protocol from the IntestiCult OGM Mouse Kit (Stemcell Technologies, Vancouver, Canada). Intestinal organoids were disassociated and resuspended into Corning® Matrigel® Growth Factor Reduced (GFR ...
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bioRxiv - Genetics 2023Quote: ... the cells were grown using the daily medium changes of STEMdiff Forebrain Neuron Differentiation kit (STEMCELL Technologies, #08600) and then transitioned to the STEMdiff Forebrain Neuron Maturation kit (STEMCELL Technologies ...
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bioRxiv - Developmental Biology 2023Quote: ... iPSCs were cultured for 4 days using STEMdiff™ Definitive Endoderm Differentiation Kit (Cat. No. 05110, STEMCELL Technologies). Media was replaced with RPMI 1640 medium ...
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bioRxiv - Genetics 2023Quote: iPSCs were differentiated into neural progenitor cells (NPCs) using the STEMdiff SMADi Neural Induction Kit (STEMCELL Technologies; #08581) Monolayer Culture Protocol following the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... For Il-2ra time course naïve CD4+ T cells were enriched from splenocytes using EasySep kit (StemCell Technologies). Approximately 100,000 cells were activated using 2ug/ml plate bound anti-CD3/CD28 ...
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bioRxiv - Immunology 2023Quote: ... B cells were purified from TMNCs with the EasySep Human CD19 Positive Selection Kit II (Stem Cell Technologies) per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The NPCs were then cultured as neurospheres in reconstituted NeuroCult® Proliferation Kit medium (STEMCELL Technologies, cat# 05702) supplemented with 20 ng/ml recombinant human EGF (STEMCELL Technologies ...
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bioRxiv - Immunology 2023Quote: ... or PEP-619WW C57BL/6 mice using a negative isolation kit (Stem Cell Technologies, Vancouver, British Columbia, Canada). A low number of isolated CD4 T cells ...
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bioRxiv - Cancer Biology 2022Quote: ... CD3+ T cells were isolated from PBMCs using EasySep™ Human T Cell Isolation Kit (StemCell Technologies, Taiwan) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... The human iPSC-derived cardiomyocytes (hiPSC-CMs) were reprogrammed using the STEMdiff™ Cardiomyocyte Differentiation Kit (STEMCELL Technologies) as recommended by the manufacturer ...
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bioRxiv - Immunology 2023Quote: Splenic B cells or T cells were Isolated with EasySepTM Mouse B Cell Isolation Kit (#19854, StemCell Technologies) or EasySepTM Mouse T Cell Isolation Kit (#19851 ...
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bioRxiv - Immunology 2023Quote: PBMCs were obtained for CD14 positive monocyte isolation using the EasySep Human Monocyte Isolation Kit (Stem cell Technologies), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... the resulting single cells were sorted on CD45 using EasySepTM Mouse CD45 Positive Selection Kit (StemCell Technologies, 18945) and used for downstream assays ...
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bioRxiv - Molecular Biology 2023Quote: ... CD34+ cells were isolated from the purified PBMNCs by EasySep Human CD34 positive selection kit II (STEMCELL Technologies) and expanded in HSC expansion media as described earlier (Genovese et al ...
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bioRxiv - Neuroscience 2024Quote: FA000010 (RUCDR/BiologyX) iPSCs were differentiated into hematopoietic precursors cells (HPCs) using the STEMdiff Hematopoietic kit (STEMCELL Technologies) largely by manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by CD34 magnetic bead-based enrichment using the EasySep human CD34 positive selection kit II (StemCell technologies). We found this enrichment strategy to be simple and reproducible and chose it for several reasons ...
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bioRxiv - Bioengineering 2023Quote: Monocytes were isolated from healthy donors’ blood using EasySep™ Human Monocyte Isolation Kit (Stemcell technologies, Cambridge, MA) by the MIT monocyte core facility ...
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bioRxiv - Immunology 2023Quote: ... CD8+ T cells were purified from P14 spleens using EasySep Mouse CD8+ T Cell Isolation Kit (Stemcell Technologies). Cells were then expanded using plate-bound CD3/CD28 (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... Neutrophils were isolated by immuno-magnetic negative selection using the EasySep Direct human neutrophil isolation kit (StemCell Technologies). A highly pure population of neutrophils was then generated by cell sorting on the basis of size ...
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bioRxiv - Neuroscience 2023Quote: ... hematopoietic lineage to obtain hematopoietic progenitor cells (HPCs) using the STEMdiff Hematopoietic Kit (StemCell Technologies, Catalog No. 05310). Next ...
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bioRxiv - Bioengineering 2024Quote: ... T-cells were then positively selected using an EasySep T-cell kit (Cat # 17951, STEMCELL Technologies, Vancouver, Canada) and an EasySep Magnet (STEMCELL Technologies ...
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bioRxiv - Neuroscience 2024Quote: ... mature microglial cells were isolated from astrocytes using EasySep Mouse CD11b Positive Selection Kit II (Stemcell Technologies #18970A,) and subsequently seeded into p60 plates ...
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bioRxiv - Immunology 2024Quote: ... Naïve T cells were isolated by EasySep™ Human Naïve Pan T Cell Isolation Kits (STEMCELL Technologies, 17961). Naïve CD8+ T cells were isolated by EasySep™ Human Naïve CD8+ T Cell Isolation Kit (STEMCELL Technologies ...
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bioRxiv - Cell Biology 2024Quote: ... bone marrow cells were further lineage depleted using EasySep™ Mouse Hematopoietic Progenitor Cell Isolation Kit (STEMCELL Technologies).
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bioRxiv - Cancer Biology 2024Quote: ... spleen-derived B-cells were selected using the EasySep™ Mouse Pan-B Cell Isolation Kit (STEMCELL Tech).
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bioRxiv - Cell Biology 2024Quote: B cells were isolated from mouse spleens using EasySep Mouse B Cell Isolation Kit (Stem Cell Technologies 19854) and attached to poly-D-lysine coated coverslips at 37°C for 1 hour ...
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bioRxiv - Cell Biology 2024Quote: ... Human CD34+ cells were magnetically enriched with the EasySep™ Human CD34 Positive Selection Kit II (STEMCELL Technologies) from either normal BM or CB samples ...
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bioRxiv - Genetics 2024Quote: ... HSPCs were purified by negative selection using the EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (Stem Cell Technologies), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... and CD14+ cells were purified using the EasySep™ Human CD14 Positive Selection Kit II (StemCell Technologies, 17858). A total of 1.5×106/well CD14+ monocytes were seeded in a 12-well plate with 50 ng/mL of hM-CSF (Peprotech ...
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bioRxiv - Bioengineering 2024Quote: ... CD34+ cells were isolated using EasySep™ Human CD34 Positive Selection Kit II (STEMCELL Technologies, Vancouver, BC, Canada) as per manufacturer instructions ...
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bioRxiv - Immunology 2020Quote: ... CAR-transduced T cells were enriched by positive selections using anti-CD19 magnetic beads (Stem Cell Technologies). Cultures were propagated for 14-16 days before applying to assays or get cryo-preserved ...
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bioRxiv - Bioengineering 2022Quote: ... Each well was rinsed with 1x PBS and an anti-adherence rinsing solution (StemCell Technologies, Aggrewell™) was added into each well ...
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bioRxiv - Cell Biology 2024Quote: ... or 1:20 Alexa488-conjugated anti-TRA-1-60 antibody (7.5 μg/mL final concentration; STEMCELL Technologies). The cells were stained in 100 μL of diluted antibody per 106 cells overnight at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... total CD8+ T cells were first enriched using the EasySep™ Mouse CD8+ T cell isolation kit (Stemcell Technologies) and then CD8+CD44hiCD62Llo central memory T cells purified by FACS ...
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bioRxiv - Cancer Biology 2021Quote: ... T cells were isolated for intravenous injection using an EasySep™ Mouse T Cell Isolation Kit (Stemcell Technologies, Canada). 4×106 isolated T cells were injected intravenously per mouse.
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bioRxiv - Systems Biology 2020Quote: ... CD66b and glycophorin A were depleted from PBMCs using magnetic sorting (Human Pan-DC Pre-Enrichment Kit, StemCell Technologies). pDCs were then sorted on a FACS Vantage instrument (BD Biosciences) ...
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bioRxiv - Immunology 2021Quote: ... Macrophages were separated from fibroblasts with the use of the EasySep Mouse selection kit (StemCell Technologies, Vancouver, BC, Canada) with mAb F4/80 conjugated to phycoerythrin.
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bioRxiv - Neuroscience 2021Quote: ... Spinal cord isolation was performed using contents of ‘NeuroCult™ Enzymatic Dissociation Kit for Adult CNS Tissue (StemCell Technologies)’ ...
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bioRxiv - Immunology 2021Quote: ... CD4+ T cells were isolated from PBMCs via the Easysep Human CD4+ T-cell negative enrichment kit (STEMCELL Technologies). The isolated human CD4+ T cells were cultured at 2.5 × 106 cells/ml in complete RPMI medium (cRPMI) ...
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bioRxiv - Immunology 2021Quote: T cells were isolated from PBMCs with EasySep™ Human T cell Isolation Kit per manufacturer’s instructions (StemCell Technologies). Isolated T cells (0.5-1 × 105 per well ...
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bioRxiv - Cancer Biology 2019Quote: ... ALDH activity in the cells was measured by flow cytometry using the ALDEFLUOR kit (Stemcell technologies, Vancouver, BC, Canada). Cells with low and high levels of ALDH enzymatic activity (respectively ALDH- and ALDH+ cells ...
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bioRxiv - Cancer Biology 2019Quote: ... we evaluated tumor propagating cell numbers in hematopoietic malignancies using an ALDEFLUOR™ kit (StemCell Technologies, Vancouver, BC, CA) to detect ALDH1+ cells ...
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bioRxiv - Cancer Biology 2019Quote: ... These cells were grown in suspension in NeuroCultTM NS-A Proliferation Kit with proliferation supplement (StemCell Technologies, Cat 05751), 20 ng/mL Recombinant Human Epidermal Growth Factor (EGF ...
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bioRxiv - Molecular Biology 2019Quote: ... Definitive endoderm (DE) was derived from a culture of H1 hESCs using the StemDiff Definitive Endoderm kit (StemCell Technologies).
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bioRxiv - Microbiology 2019Quote: ... B cells were isolated from PBMCs through negative enrichment using EasySep Human B cell isolation kit (Stem Cell Technologies) following manufacturer’s protocol ...
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bioRxiv - Genetics 2019Quote: ... 5×104/mL bone marrow and 5×105/mL spleen cells were cultured with MegaCult-C Kit (STEMCELL TECHNOLOGIES) for 9 days ...
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bioRxiv - Immunology 2021Quote: ... Poly I:C-activated NK cells were isolated from the spleen using EasySep Mouse NK Cell Isolation Kit (STEMCELL Technologies). Purified NK cells were co-cultured with target cells at a ratio of 5:1 ...