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Citations for Stemcell Technologies :
701 - 750 of 4617 citations for Rat T Cell Surface Glycoprotein CD3 Gamma Chain CD3G ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2021Quote: Primary CD4+ and CD8+ T cells were isolated from anonymous donor blood after apheresis by negative selection (STEMCELL Technologies #15062 & 15063). Blood was obtained from Blood Centers of the Pacific (San Francisco ...
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bioRxiv - Bioengineering 2021Quote: ... T cells were allowed to recover in antibiotic-free medium containing 1 µg/mL DNase I solution (STEMCELL Technologies, Cambridge, MA) at 37 °C ...
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bioRxiv - Immunology 2021Quote: ... Spleens of recipient mice were collected 8 days after viral infection and CD8+ T cells were purified by magnetic bead selection (EasySep, STEMCELL Technologies). Fluorescence-activated cell sorting is used for the separation of tdTomato OT-I CD8+ cells from the CD8+ T cells of recipient WT mice.
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bioRxiv - Immunology 2022Quote: ... We used a method of MAIT cell expansion as previously described13 with the following modifications: instead of using base medium of Immunocult-XF T cell expansion medium (Stemcell Technologies) we used RPMI-1640 and substituted CTS serum (Invitrogen ...
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bioRxiv - Biophysics 2023Quote: NK-92 (ATCC®, CRL-2407™) were cultured in the ImmunoCult-XF T Cell Expansion Medium (STEMCELL Technologies Inc., USA) with the addition of Human Recombinant Interleukin 2 (IL-2 ...
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bioRxiv - Cell Biology 2023Quote: Primary human CD4+ and CD8+ T cells were isolated from commercially available whole human blood (STEMCELL Technologies Inc., USA, Cat# 70507.1) with EasySep Human CD4+ or CD8+ T Cell Isolation Kits (STEMCELL Technologies Inc. ...
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bioRxiv - Immunology 2022Quote: Splenic B cells isolated with EASYSEP MOUSE B cell isolation kit (Stem Cell Technologies) were cultured at 1.10^6 cells per ml in RPMI 1640 medium (Eurobio ...
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bioRxiv - Immunology 2020Quote: Primary CD4+ T-cells for in-vitro HIV-1 infection were isolated from total blood of healthy individuals using the RosetteSep™ Human CD4+ T Cell Enrichment Cocktail (STEMCELL Technologies Inc. ...
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bioRxiv - Pathology 2023Quote: ... CD4+ T cells were isolated from 40 ml of peripheral blood from HIV-1-infected individuals on ART by immunodensity negative-selection using the RosetteSep™ Human CD4+ T Cell Enrichment Cocktail (STEMCELL Technologies). Cells were plated at a concentration of 1-3×106/ml in RF10 and stimulated with 2 ng/ml PMA for 16 h when indicated ...
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bioRxiv - Immunology 2024Quote: ... splenic P14 TM were enriched at indicated time points after LCMV Arm by removal of APC- or PE-labeled CD4+ T and CD19+ B cells with anti-APC/PE magnetic microbeads (Miltenyi Biotec & STEMCELL Technologies) and 5×103 P14 TM were transferred i.v ...
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bioRxiv - Bioengineering 2024Quote: ... CD3+ primary human T-cells were isolated by negative selection as per manufacturer’s instructions (Cat # 15021, 15061, RosetteSep Human T-cell Enrichment Cocktail, STEMCELL Technologies, Vancouver, Canada). T-cell pellets were resuspended in dilution medium and counted using a hemocytometer with 0.4% Trypan blue viability stain (ThermoFisher Scientific ...
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bioRxiv - Immunology 2019Quote: ... Cells were then permeabilized with 0.3% Triton-X for 15 min followed by 30 min block in 0.1% Triton-X + 5% normal rat serum (Stem Cell Technologies). Cells were incubated in Drp1 p616 Ab overnight at 4°C in 0.1% Triton-X + 1% BSA and then in secondary antibodies (AF488 Goat anti-Rabbit) ...
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bioRxiv - Developmental Biology 2023Quote: ... StemLight Pluripotency Antibody Kit (Stem Cell Technologies) was used for pluripotency testing ...
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bioRxiv - Bioengineering 2022Quote: Plasma was extracted from surface layer by using SepMate-15 tubes with Lymphoprep gradient medium (StemCell Technologies) after centrifugation at 1200g for 20 minutes ...
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bioRxiv - Developmental Biology 2021Quote: hPSCs are released from well-plate surfaces using non-enzymatic agitation following manufacturer’s instructions (ReleSR, STEMCELL Tech.). Cells are resuspended as a single-cell suspension at densities of 750K-1M cells/mL in mTeSR1 containing 10μM ROCK inhibitor Y27632 (Abcam) ...
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bioRxiv - Immunology 2022Quote: ... NK cells were isolated using the EasySep Human NK Cell Isolation Kit (Stem Cell Technologies) following manufacturer instructions ...
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bioRxiv - Immunology 2022Quote: Primary human CD8+ T cells were isolated from buffy coats of healthy donors and purified (>95%) by negative selection using the RosetteSep™ Human CD8+ T Cell Enrichment Cocktail (StemCell Technologies, #15063), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: Cells were dissociated using the CM dissociation kit (Stem Cell Technologies, #05025). After centrifugation for 3 min at 130g ...
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bioRxiv - Immunology 2022Quote: ... beads selectively bound to IgA through kappa light chain were separated from the supernatant with EasySep magnetic stand (StemCell technologies). Supernatant was discarded and beads washed 3 times ...
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bioRxiv - Cancer Biology 2020Quote: ... CellSep Human CD19+ selection kit (Stem Cell Technologies) was used to enrich for CD19+ cells ...
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Salinomycin inhibits epigenetic modulator EZH2 to enhance Death Receptors in Colon Cancer Stem CellsbioRxiv - Cancer Biology 2020Quote: ... Aldefluor kit was bought from Stem cell technologies. Magnetic ChIP kit (Cell Signaling Technology) ...
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bioRxiv - Bioengineering 2020Quote: Negative selection using RosetteSep kits (Stem Cell Technologies) was adopted to isolate primary human T cells from healthy volunteer donors following leukapheresis ...
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bioRxiv - Genetics 2020Quote: ... STEMdiff Cerebral Organoid kit (Stem Cell Technologies, 08570) was used for organoid culture with timing according to manufacturer’s suggestion and Matrigel embedding as previously described57 ...
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Chimeric Antigen Cytotoxic Receptors for In-Vivo Engineering of Tumor-targeting Natural Killer CellsbioRxiv - Immunology 2023Quote: ... ImmunoCult™ NK cell-expansion kit (StemCell Technologies) was used following the manufacturer’s recommendations to activate isolated NK cells in vitro for 6 days ...
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bioRxiv - Immunology 2021Quote: ... B cells were isolated from single cell suspensions by using negative selection kit (Stem Cell Technologies) prior to flow cytometry and cell sorting.
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bioRxiv - Immunology 2022Quote: B cells from spleen were sorted with EASYSEP MOUSE B cell isolation kit (Stem Cell Technologies) and lysed using RIPA buffer (Santa Cruz ...
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bioRxiv - Cancer Biology 2023Quote: ... Lineage-negative cells were enriched using EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (Stem Cell Technologies) according to manufacturer’s protocol prior to sorting ...
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bioRxiv - Immunology 2023Quote: ... CD4+ T cells were purified from donor 2 LRS cone using a Rosettesep human CD4+ enrichment cocktail (Stemcell Technologies, Cambridge, MA; Supplementary Fig. 1B). 5×106 CD4+ T cells from donor 2 were cultured with 5×105 DCreg from donor 1 in X-vivo media for 5 days ...
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bioRxiv - Biophysics 2019Quote: ... Cells were passaged by dissociation with Neurocult Chemical Dissociation Kit (Stem Cell Technologies). For immunostaining ...
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bioRxiv - Immunology 2023Quote: ... B cells were enriched (Human Pan-B cell Enrichment Kit [19554, StemCell Technologies]) and then stained with viability dye FVS780 (565388 ...
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bioRxiv - Immunology 2023Quote: ... the dead cells were first removed with a Dead Cell Removal Kit and then the NK cells were depleted using the EasySep Human CD56 Positive Selection Kit (Stemcell Technologies). The control 221 cells were kept in the same culture conditions but were not exposed to NK cells ...
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bioRxiv - Genomics 2022Quote: ... line AICS-0024) are released from well-plate surfaces using non-enzymatic agitation following manufacturer’s instructions (ReleSR, STEMCELL Tech.). Cells are resuspended as a single-cell suspension at densities of 750K-1M cells/mL in mTeSR1 containing 10 μM ROCK inhibitor Y27632 (Abcam ...
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bioRxiv - Immunology 2019Quote: ... B cells were purified using the EasySep negative selection mouse B cell enrichment kit (Stem Cell Technologies). B cell purity was assessed by flow cytometry using anti CD19 or B220 antibodies (we routinely observed over 98% purity) ...
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bioRxiv - Cell Biology 2021Quote: Endodermal cells were induced from cynIPS cells using STEMdiff Definitive Endoderm (DE) Kit (Stem Cell Technologies, 05110). CynIPS cells were seeded onto a 24-well plate (TPP ...
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bioRxiv - Immunology 2021Quote: ... samples were enriched for live cells using a Dead Cell Removal (Annexin V) Kit (Stem Cell Technologies), then labeled with unique anti-human TotalSeq-B hashing antibodies (BioLegend) ...
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bioRxiv - Molecular Biology 2021Quote: Cell growth monitoring and analysis of hESCs trilineage differentiation were performed using the Cell Counting Kit-8 (Dojindo) and STEMdiffTM trilineage differentiation kits (Stem Cell Technology), according to the manufacture’s protocols ...
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bioRxiv - Cell Biology 2021Quote: ... c-Kit+ cells were stained and magnetically isolated from the lineage-depleted cell suspension using the EasySep mouse CD117 (c-Kit) positive selection kit (Stem Cell Technologies), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Splenic B cells were isolated using a mouse B cell isolation kit (STEMCELL Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... live cells were purified using EasySep Dead Cell Removal (Annexin V) Kit (StemCell Technologies). The remaining viable cells were further incubated in complete media without CRYBMIM for 6-9 days with media replacement every 3 days ...
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bioRxiv - Immunology 2020Quote: ... splenic B cells were purified with a mouse B cell isolation kit (StemCell Technologies), and then stimulated with lipopolysaccharide (LPS ...
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bioRxiv - Cancer Biology 2023Quote: ... dead cell exclusion was performed using an EasySep™ separation kit (Stem Cell Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... CD90+ cells were then isolated using the CD90.2 Cell Isolation Kit (Stemcell Technologies, 18951). Two days after CD90+ cells were purified ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were passaged as necessary using MesenCult-ACF Dissociation Kit (Stem Cell Technologies, 05426). Cells were subcultured onto Matrigel at the first passage to avoid loss of the differentiated cells before tolerating the switch to MesenCult-ACF attachment substrate (Stem Cell Technologies ...
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bioRxiv - Cancer Biology 2021Quote: ... using the Mammary epithelial cell enrichment kit (StemCell Technologies). 1×106 cells were injected into the right inguinal mammary fat pads of FVB/N mice ...
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bioRxiv - Microbiology 2022Quote: ... and EasySep negative isolation kits (Stem Cell Technologies, #19054) were used to sequentially isolate B-cells ...
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bioRxiv - Cancer Biology 2022Quote: ... using the Mammary epithelial cell enrichment kit (StemCell Technologies). 1×105 cells were injected into the right inguinal mammary fat pads of nude mice ...
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bioRxiv - Cancer Biology 2022Quote: ... a PE sorting kit (#18554, Stem Cell Technologies, BC) was used ...
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bioRxiv - Cell Biology 2023Quote: ... intestinal epithelial organoid culture kit protocol (Stem cell technologies) was followed as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... 1 × 105 human NK cells isolated from PBMC using EasySep human NK cell isolation kit (Stem Cell Technologies) were added to wells in the presence of CD107a PE (BD ...
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bioRxiv - Cell Biology 2024Quote: B cells were isolated from mouse spleens using EasySep Mouse B Cell Isolation Kit (Stem Cell Technologies 19854) and attached to poly-D-lysine coated coverslips at 37°C for 1 hour ...