Labshake search
Citations for Stemcell Technologies :
701 - 750 of 1640 citations for Mouse Histone H3.3C H3 5 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... supplemented NeuroCult NS-A proliferation kit (human, STEMCELL Technologies, #05751), human recombinant EGF (20 ng/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... we used the STEMdiff™ Hematopoietic Kit (Stem Cell Technologies). Briefly ...
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bioRxiv - Developmental Biology 2023Quote: ... and the hPSC Genetic Analysis Kit (Stemcell Technologies, Vancouver, Canada). Interphase and M phase nuclei were also screened via FASTFISH (WiCell ...
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bioRxiv - Immunology 2023Quote: ... with EasySep Human CD4+ T Cell Isolation Kit (STEMCELL Technologies). Enriched CD4+ cells were stained for 30 min on ice with antibodies against anti-CD4 ...
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bioRxiv - Neuroscience 2023Quote: ... cells were incubated with CD4 negative selection kit (STEMCELL Technologies) for 15 min on ice and CD4- cells were magnetically removed ...
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bioRxiv - Bioengineering 2023Quote: ... MSCs were cultured in MesenCult Adipogenic Differentiation Kit (StemCell Technologies) for 14 days ...
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bioRxiv - Bioengineering 2023Quote: ... provided in the STEMdiff™ Definitive Endoderm Kit (STEMCELL Tech.). At day -1 ...
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bioRxiv - Microbiology 2023Quote: ... and STEMDiff™ Neural Organoid Maintenance Kit (STEMCELL Technologies™) according to the manufacturer’s protocols with minor modifications ...
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bioRxiv - Immunology 2024Quote: ... isolation kit and EasyPlate EasySep magnets (Stem Cell Technologies, 18102) or EasyEights EasySep magnets (Stem Cell Technologies ...
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bioRxiv - Developmental Biology 2024Quote: ... The timing of the kit protocol (StemCell Technologies Document #DX21849) was followed ...
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Chimeric Antigen Cytotoxic Receptors for In-Vivo Engineering of Tumor-targeting Natural Killer CellsbioRxiv - Immunology 2023Quote: ... EasySep™ Human NK cell-negative isolation kit (StemCell Technologies) was used to isolate human peripheral blood NK cells from healthy donor leukopaks ...
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Chimeric Antigen Cytotoxic Receptors for In-Vivo Engineering of Tumor-targeting Natural Killer CellsbioRxiv - Immunology 2023Quote: ... EasySep™ Human T-cell-negative isolation kit (StemCell Technologies) was used to isolate peripheral blood T cells from healthy donor leukopaks ...
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bioRxiv - Immunology 2024Quote: ... using EasySep™ Human NK Cell Isolation Kit (Stemcell Technologies). The isolated NK cells were incubated overnight at 37°C 5% CO2 in R10 (RPMI-1640 (Sigma Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... at 37°C for 5 minutes and replated in a ratio of 1:6 or 1:9 on new Matrigel-coated plates in E8 supplemented with 5 μM ROCK inhibitor Y-27632 (Stemcell Technologies, Canada) for 24h ...
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bioRxiv - Genetics 2019Quote: 1×10^5 cells from the RF of xenotransplanted mice were transferred to 1ml of MethoCult H4034 Optimum methylcellulose medium (StemCell Technologies, 04034) and plated onto a 35mm dish for human specific colony formation ...
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bioRxiv - Cancer Biology 2020Quote: ... containing 10% fetal bovine serum and subsequently resuspended at a concentration of 5×105 cells/mL in 4-5mL of MethoCult H4230 methylcellulose (STEMCELL Technologies, 04230) containing penicillin/streptomycin ...
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bioRxiv - Bioengineering 2021Quote: ... we first patterned the hiPSCs with a mesenchymal protocol as previously described.(Lam, et al., 2014) Cells were treated with 5 µM CHIR99021 (STEMCELL Technologies, 72052) in Roswell Park Memorial Institute Medium 1640 (RPMI 1640 ...
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bioRxiv - Bioengineering 2021Quote: hiPSC-derived endothelial cells (hECs) were differentiated as previously described.[85] hiPSCs were subjected to 5 μM CHIR99021 in STEMdiff APEL 2 Medium (STEMCELL Technologies, 05270) for 24 hours ...
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bioRxiv - Bioengineering 2021Quote: ... we first directed the hiPSCs towards an intermediate mesenchymal fate using a previously described protocol,[84] wherein cells are treated with 5 µM CHIR99021 (STEMCELL Technologies, 72052) in Roswell Park Memorial Institute Medium 1640 (RPMI 1640 ...
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bioRxiv - Neuroscience 2021Quote: ... These cells were cultured in mTeSR™1 medium (mTeSR™1 Basal Medium with mTeSR™1 5× Supplement (Stemcell Technologies) and 1% penicillin-streptomycin (P/S ...
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bioRxiv - Cell Biology 2020Quote: ... Neural rosettes were derived between day 3 to day 5 by addition of STEMdiffTM Neural Rosette Selection Reagent (05832, Stem Cell Technologies) for 45 mins-1 hr at 37oC ...
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bioRxiv - Molecular Biology 2022Quote: ... The differentiation was started following 24 h of seeding and proceeded through a 7-stage differentiation protocol (stages 1 – 4 in adherent culture, stage 5 in AggreWell (Stemcell Technologies, #34421), and stages 6 and 7 in suspension culture).
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bioRxiv - Microbiology 2022Quote: ... HCT-8 cells were cultured in 25 cm flasks with 5 ml of complete media (RPMI-1640 media [Gibco/Thermo Fisher Scientific, Waltham, MA] supplemented with 10% fetal bovine serum [Stemcell Technologies, Vancouver ...
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bioRxiv - Cell Biology 2022Quote: ... Thawed cells were cultured at 37°C and 5%O2 in serum-free HSC media comprised of StemSpan II medium (Stem Cell Technologies) supplemented with CC100 cytokine cocktail (Stem Cell Technologies) ...
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bioRxiv - Neuroscience 2019Quote: ... followed immediately by engrafting 1-5×104 human umbilical cord CD34+ stem cells (Lonza Incorporated, Walkersville, MD; or Stemcell Technologies, Vancouver, BC) via intrahepatic injection ...
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bioRxiv - Immunology 2021Quote: ... Cells were fed for 4-5 days until airlifted and continued to be grown at air-liquid interface (ALI) with PneumaCult ALI medium (StemCell Technologies, #05001) until fully differentiated (4 weeks)
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bioRxiv - Cell Biology 2023Quote: ... monolayers were washed with PBS and then incubated with 500 μL dispase (5 U/ml) in Hank’s balanced salt solution (Stemcell Technologies, Cat. #07913) for 30 min at 37°C ...
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bioRxiv - Developmental Biology 2023Quote: ... The cell culture medium was supplemented with 1:200 Rho-associated protein kinase inhibitor (ROCKi) Y-27632 (final concentration 5 µM, STEMCELL Technologies, 72302) on the first day after passage ...
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bioRxiv - Molecular Biology 2023Quote: E14 and RPL39L KO cells were treated for 5h at 37°C and 5% CO2 with 10μM (S)-MG132 (STEMCELL Technologies Catalog #73264) and 5μM Bafilomycin-A1 (STEMCELL Technologies Catalog #74242 ...
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bioRxiv - Bioengineering 2023Quote: ... we washed 80-90% confluent cultures with PBS-and dissociated cultures into a single-cell suspension with 5 min of Accutase (StemCell Technologies Inc.) treatment at 37°C ...
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bioRxiv - Bioengineering 2023Quote: ... Single cells were centrifuged at 200g for 5 minutes and resuspended in StemFlex supplemented with 10 µM Y-27632 (StemCell Technologies Inc.). We seeded 5.5×106 cells per 5.5 mL of mTeSR+ in each well of a six-well suspension plate ...
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bioRxiv - Cell Biology 2023Quote: ... The hESCs H1 cell-lines were differentiated using the extended 7-stage protocol (stages 1–4 in adherent culture, stage 5 in AggreWell (Stemcell Technologies, #34421), and stages 6 and 7 in suspension culture).
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bioRxiv - Developmental Biology 2023Quote: ... Four-month-old cortical organoids were randomly selected and transferred to a recording chamber kept at 37L°C using a heating platform and a controller (TC-324C, Warner Instruments) in 5% methyl-cellulose in BrainPhys Imaging Optimized Medium (STEMCELL Technologies, #05796). Imaging was performed using a SP-8X microscope with a multiphoton laser ...
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bioRxiv - Developmental Biology 2023Quote: ... and maintained at 37C with 5%CO2 with daily removal of differentiated cells and replacement of mTeSR1 media (STEMCELL Technologies, Vancouver Canada). Cells were passaged routinely every 4 days using Dispase (STEMCELL Tec.) ...
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bioRxiv - Cell Biology 2024Quote: ... Then cells were seeded at 70% confluency on dishes pre-coated with Matrigel and cultured in StemMACS iPS-Brew XF + 5 μM Y-27632 (STEMCELL Technologies 72304). On D1 ...
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bioRxiv - Genetics 2024Quote: ... CD34+ HSPCs were cultured at 1×105–5×105 cells/mL in StemSpan Serum-Free Expansion Medium II (STEMCELL Technologies, Vancouver, Canada) or Good Manufacturing Practice Stem Cell Growth Medium (SCGM ...
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bioRxiv - Bioengineering 2021Quote: ... A 3x103 cell fraction of non-transduced and transduced Lin-cells were transferred into 3 ml methylcellulose-based medium with mouse cytokines optimized for hematopoietic progenitor cell differentiation (StemCell Technologies) and plated at 1 mL/well for a CFU assay ...
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bioRxiv - Microbiology 2021Quote: ... 5% CO2 for 15-20 minutes to allow the Matrigel to polymerize and then 550 μl of pre-warmed complete IntestiCult Growth Medium (mouse) (6005; STEMCELL Technologies) containing 500 nM Y-27632 (10005583 ...
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bioRxiv - Molecular Biology 2022Quote: ... Isolated cells were grown in a semi-solid methyl cellulose matrix for the Mouse Hematopoietic CFU Assay in Methocult Media 3630 (STEMCELL Technologies), following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: Methylcellulose-based hematopoietic progenitor assay was routinely used to enumerate haematopoietic progenitors from both mouse (MethoCult™ GF M3434, Stemcell Technologies) and human (MethoCult™ H4434 ...
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bioRxiv - Microbiology 2023Quote: ... the mice were irradiated with a dose of 100 cGy per mouse and injected intravenously with 0.2 million of HSCs (purchased from Stemcell Technologies Inc.). After 8–10 weeks ...
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bioRxiv - Neuroscience 2023Quote: Nodose ganglia were dissected from Snca-/- mice and placed in 300 μL ice cold mouse Intesticult media (StemCell Technologies, Cat#06005) containing nerve growth factor-2 (NGF ...
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bioRxiv - Developmental Biology 2023Quote: CFU assays were performed according to the Mouse “Colony-Forming Unit” (CFU) Assays Using MethoCult™” GF M3434 protocol (StemCell Technologies) (STEMCELL) ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then washed with basal medium (500g, 4°C) and replated in Matrigel in mouse IntestiCult Organoid Growth Medium (STEMCELL Technologies) supplemented with Y-27632 (10 μM) ...
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bioRxiv - Immunology 2024Quote: The M210B4 stromal cell line was isolated from normal mouse bone marrow adherent cells50 and maintained in RPMI 1640 medium (STEMCELL Technologies) with 10% FBS ...
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bioRxiv - Cancer Biology 2021Quote: EasySep human CD45 Depletion kit II (Stem Cell Technology, Cat#: 17898) was used to extract immune cells from freshly dissected patient tumors ...
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bioRxiv - Immunology 2021Quote: ... Naïve T cells were isolated using negative selection kits (Stemcell Technologies).
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bioRxiv - Neuroscience 2021Quote: ... (2017)57 or the newly available differentiation kit by STEMCELL Technologies based on the McQuade et al ...
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bioRxiv - Bioengineering 2021Quote: ... using EasySep™ human T cell isolation kit (#17951, StemCell Technologies). 10,000 Raji cells were mixed with 100,000 T cells in media containing different concentrations of purified BiTE clones (Clone 5 and Clone 6) ...
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bioRxiv - Cell Biology 2022Quote: ... after dissociation of aggregates with Stemdiff CM dissociation kit (STEMCELL Technologies) following the manufacturer’s protocol after adding Accutase to the enzyme mix (1:2) ...