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Citations for Stemcell Technologies :
701 - 750 of 1419 citations for Human integrin alpha 5 beta 3 His tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... The NSC media was composed of Neurocult NS-A basal medium human (Stem Cell Technologies, Cat #05750) along with L-glutamine (Sigma ...
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bioRxiv - Microbiology 2019Quote: ... and CD4+ T cells were isolated using EasySep Human CD4+ T cell isolation kit (StemCell Technologies #17952). Cells were resuspended to 2.5 × 106 cells/mL in RPMI complete media supplemented with 10% FBS ...
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bioRxiv - Immunology 2020Quote: ... Plasma samples were diluted by half with PBS during RosetteSep human B cell processing (StemCell Technologies #15064), aliquoted ...
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bioRxiv - Immunology 2021Quote: Primary human CD3+ T cells were isolated from donor PBMCs via immunomagnetic negative selection (Stem Cell Tech). For thaw and culture ...
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bioRxiv - Immunology 2021Quote: ... NK cells were isolated from PBMCs using EasySep™ Human NK Cell Isolation Kit (Stemcell Technologies, BC). NK cells were co-cultured with K562-NL cells at different E:T ratios and treated with 10 μM of colistin for 5 hours at 37°C ...
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bioRxiv - Immunology 2021Quote: ... Primary CD8+ T cells were isolated using EasySep Human CD8+ T Cell Enrichment Kits (Stem Cell Technologies) and cultured in RPMI-1640 (ATCC ...
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bioRxiv - Immunology 2022Quote: ... Monocytes were purified from the PBMC population using the EasySep Human monocyte enrichment kit (STEMCELL Technologies; 19059), plated in 96-well plates and differentiated into microglia (iMDM ...
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bioRxiv - Immunology 2022Quote: ... coated with human type IV placental collagen in submerged PneumaCult-Ex Plus media (StemCell Technologies, Vancouver, Canada). When cells reached confluency ...
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bioRxiv - Neuroscience 2022Quote: ... human iPSCs were dissociated into single cells using Gentle Cell Dissociation Reagent (STEMCELL Technologies catalog # 100-0485) and were grown for 1 day with STEMdiff Neuronal induction medium (STEMCELL Technologies catalog # 05835) ...
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bioRxiv - Neuroscience 2022Quote: ... human iPSCs were routinely passaged as clumps onto Matrigel-coated plates with mTeSR+ media (StemCell Technologies #85850) according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... CD3+ T cells were isolated from PBMCs using EasySep™ Human T Cell Isolation Kit (StemCell Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... naïve CD4 T cells were purified using the naïve human CD4+ T cell isolation kit (StemCell Technology) and stimulated with immobilized anti-human CD3 (5 μg/ml ...
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bioRxiv - Immunology 2023Quote: Human PBMCs were isolated from buffy coats by density gradient centrifugation (Lymphoprep®, Stemcell Technologies Cat# 07811) and cultured overnight in T-cell medium (CTS OpTmizer® ...
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bioRxiv - Cancer Biology 2023Quote: ... T cells were negatively purified with EasySepTM beads (human T-cell isolation kit, STEMCELL Technologies, Vancouver, Canada). T cells were cultured in Advanced RPMI 1640 enriched with FBS (10%) ...
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Efficient Methods for Target Gene Manipulation in Haematopoietic Stem Cell Derived Human NeutrophilsbioRxiv - Cell Biology 2023Quote: ... medium was changed to SFEM II containing 10ng/mL recombinant human granulocyte colony stimulating factor (StemCell Technologies) and maintained in this medium until day 20 ...
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bioRxiv - Microbiology 2023Quote: ... CD4+ T cells were isolated using the EasySep Human CD4+ T cell Isolation Kit (Stemcell Technologies, 17952) and subsequently activated using the T Cell Activation/Expansion Kit (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2023Quote: Human iPSCs were differentiated for 12 days using the STEMdiff™ Hematopoietic Kit (#05310, STEMCELL Technologies, Inc.) [43] ...
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bioRxiv - Immunology 2023Quote: Neutrophils of healthy blood donors were isolated using EasySep™ Direct Human Neutrophil Isolation Kit (Stemcell Technologies). Neutrophils were stimulated with immune-complexes as described above and supernatants collected after 4 h ...
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bioRxiv - Immunology 2023Quote: ... B cells were isolated using an EasySep Human Pan-B Cell Enrichment kit (Stemcell Technologies, Vancouver, Canada).
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bioRxiv - Cancer Biology 2023Quote: ... The human MLL-AF9-transduced cells were cultured in StemSpan SFEM II medium (#ST-09655, STEMCELL Technologies) containing 1% penicillin– streptomycin together with 10 ng/ml rhSCF (#255-SC ...
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bioRxiv - Immunology 2023Quote: ... Cells were enriched for CD8+ population using the EasySep Human CD8+ T Cell Isolation Kit (Stemcell Technologies) and CD8+ cells were used for analysis.
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bioRxiv - Genomics 2023Quote: Primary CD25-CD4+ effector T cells were isolated from fresh Human Peripheral Blood Leukopaks (STEMCELL Technologies, #70500) from healthy donors ...
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bioRxiv - Immunology 2023Quote: ... and monocytes were isolated from PBMCs by EasySep Human T Cell Isolation Kit (StemCell Technologies, Vancouver, Canada), MACSxpress Whole Blood NK Cell Isolation Kit (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2023Quote: ... with the addition of 1 μg/L of human interleukin 2 (IL-2, STEMCELL Technologies Inc., USA).
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bioRxiv - Cell Biology 2023Quote: H9 human embryonic stem cells (hESCs; female, WA09, WiCell) were cultured in mTeSR Plus medium (STEMCELL Technologies) with 1% Penicillin-Streptomycin in 8 mg/mL Matrigel-coated (Corning ...
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bioRxiv - Synthetic Biology 2023Quote: ... and ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator (25 µL/mL) (Stemcell Technologies, Cat.#10990) and cultured for 1.5 days at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2023Quote: ... and T cells were purified using the EasySep™ Human T Cell Isolation Kit (StemCell Technologies, 17951). Once purified ...
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bioRxiv - Cell Biology 2024Quote: ... Epithelial cells were enriched using the EasySep™ Human EpCAM Positive Selection Kit II (Stemcell Technologies, #17846) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Naïve CD4+ T cells were isolated using EasySep Human Naïve CD4+ T Cell Isolation Kit (Stemcell technologies), according to manufacturer’s indications.
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bioRxiv - Bioengineering 2024Quote: ... T cells were isolated using the EasySepTM Human T cell isolation kit (StemCell Tech., Saint-Egrève, France) and cultured in complete X-Vivo15 medium ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were then treated with 20 uL/mL of Immunocult Human Treg Differentiation Supplement (StemCell Technologies) containing recombinant human TGF-B1 and all-trans retinoic acid ...
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bioRxiv - Neuroscience 2022Quote: ... IPSCs were cleaned and 1/3 dish was gently dissociated with dispase (Stemcell Technologies # 07923) for 12-15 minutes at 37°C ...
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bioRxiv - Immunology 2020Quote: ... FcγR-specific mAbs were obtained from Stem Cell technologies (CD16: clone 3G8; CD32: IV.3). Reverse sICs were generated from these receptor-specific antibodies using goat-anti-mouse IgG F(ab)2 fragments (Invitrogen ...
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bioRxiv - Developmental Biology 2021Quote: ... Plates were pre-coated with coated with 3 μg/ml of vitronectin (07180, Stemcell Technologies) and 5mg/mL Col IV (C7521-5MG ...
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bioRxiv - Immunology 2021Quote: Female C57BL/6 mice received a single dose of mouse recombinant CSF-3 (StemCell Technologies) at 0.25mg/kg in sterile PBS ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 × 106 cells were added per well in the AggreWell 800 plate (STEMCELL Technologies, 34815) with Essential 8™ Flex Media supplemented with Stemgent hES Cell Cloning & Recovery Supplement (1X ...
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bioRxiv - Neuroscience 2023Quote: ... Approximately 3 million cells were transferred to each well in AggreWell 800 (STEMCELL Technologies, 34815) and centrifuged at 100g for 3 minutes in hiPSC medium supplemented with ROCK inhibitor Y-27632 (EMD Chemicals) ...
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bioRxiv - Cancer Biology 2023Quote: Primary cells are resuspended in a 3:4 solution of Mammocult medium (Stem Cell Technology) and Matrigel (Corning)17,26,29 ...
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bioRxiv - Bioengineering 2023Quote: ... these cells were used in 3 mL of methylcellulose medium (H4435; Stem Cell Technology, Canada) plated in a 35-mm culture dish (Grainger) ...
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bioRxiv - Immunology 2023Quote: ... mice were given a daily 3 µmol/kg intraperitoneal injection of dibenzazepine (DBZ) (StemCell Technologies) which was first dissolved as a 10 µmol/ml in DMSO stock solution (kept frozen at -20C ...
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bioRxiv - Bioengineering 2023Quote: ... and passaged every 3-4 days using Gentle Cell Dissociation Reagent (STEMCELL Technologies, 100-0485) for clump passaging ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were passaged every 3-4 days by dissociating them using Accutase (STEMCELL Technologies) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... BMPCs were enriched from bone marrow mononuclear cells using EasySep Human CD138 Positive Selection Kit II (StemCell Technologies) and immediately used for ELISpot or cryopreserved in 10% dimethyl sulfoxide in FBS.
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bioRxiv - Immunology 2021Quote: ... B cells were enriched from PBMC by first using EasySep Human Pan-B cell Enrichment Kit (StemCell Technologies), and then stained with CD20-PB (2H7 ...
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bioRxiv - Immunology 2021Quote: ... NK cells were isolated from whole blood with the EasySepTM Direct Human NK Cell Isolation Kit (StemCell Technologies). The gating strategy for isolation of CD56+CD3- NK cells is shown in Figure S1 (Figure 2 – Supplement 1) ...
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bioRxiv - Developmental Biology 2020Quote: Human embryonic stem cells (hESCs; H9 line) were cultured in mTeSR™1 basal medium (Stem Cell Technologies), in 6-well plates coated with matrigel (Corning Matrigel Matrix hESC-qualified) ...
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bioRxiv - Immunology 2022Quote: ... CD14+ CD16- monocytes were isolated using magnetic negative selection (EasySep Human Monocyte Isolation Kit, StemCell Technologies, Cambridge, Massachusetts) and differentiated into macrophages by culture in RPMI 1640 (Lonza ...
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bioRxiv - Cancer Biology 2020Quote: Frozen human bone marrow mononuclear cells were thawed and washed twice with Iscove’s Modified Dulbecco’s Medium (StemCell Technologies) containing 10% fetal bovine serum (FBS) ...
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bioRxiv - Immunology 2020Quote: ... Monocytes were isolated from PBMC by negative selection using the EasySep™ Human Monocyte Isolation Kit (STEMCELL Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: T and B cell lineage depletion was performed using RosetteSep Human Hematopoietic Progenitor Cell Enrichment Cocktail (Stemcell Technologies) and Ficoll-Paque density gradient centrifugation from routine red cell exchange apheresis performed at Texas Children’s Hospital or Columbia University New York Presbyterian Hospital ...