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Citations for Stemcell Technologies :
701 - 750 of 2158 citations for Estradiol Serum ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... were seeded into the wells of 96-well plates (Costar, flat bottom) and maintained in Pneuma-Cult Expansion medium (PC-Ex) (STEMCELL TECHNOLOGIES) till confluency ...
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bioRxiv - Neuroscience 2023Quote: ... sorted into 96-well plates using a WOLF benchtop microfluidic cell sorter (Nanocellect) in mTeSR Plus and CloneR (Stemcell Technologies, 05888). After thirteen days ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were cultured in feeder-free medium and grown on Matrigel-coated plates in cell medium containing 1:1 mTesR1: Essential 8 (StemCell Technologies, 85850 and Thermofisher A1517001 ...
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bioRxiv - Microbiology 2023Quote: ... A final boost in PBS was administered and 4 days later splenocytes were fused with Sp2/10 Ag14 cells (ATCC CRL-1581) and cultured overnight prior to concurrent cloning and HAT selection on methylcellulose plates (ClonaCell-HY, StemCell Technologies). After 14 days of incubation at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... and the cells were then replated on matrigel-coated plates in mTesR plus media at a density of 40,000 cells/cm2 (Stem Cell Technologies). Cells were allowed to proliferate for two days with a daily media change ...
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bioRxiv - Molecular Biology 2022Quote: ... T cells were seeded at 2 × 106 cells/mL (typically 10×106 cells per well of a 6-well plate) and activated with ImmunoCult T Cell activator (STEMCELL Technologies) following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... Current protocol is optimized for 16 bEPSCs and 16 bTSCs per well in a ∼1200 well Aggrewell 400 microwell culture plate (Stemcell technologies) for a total of 19,200 of each cell types per well ...
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bioRxiv - Neuroscience 2023Quote: ... NPCs were digested with Accutase and then differentiated onto PLO/laminin coated plates/coverslips with final differentiation media which is BrainPhys media (STEMCELL Technologies) supplemented with Glutamax (Fisher) ...
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bioRxiv - Neuroscience 2023Quote: ... Six-well plates coated for at least 2h with 10µg/mL of Cell Adhere Laminin 521 (Stem Cell Technology, 200-0117) diluted in DPBS containing calcium and magnesium (Corning ...
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bioRxiv - Physiology 2023Quote: ... Cells were then plated between 450,000 - 750,000 cells per well of a Matrigel coated 12-well tissue culture plate in mTeSR1 PLUS™ (Stem Cell Technologies) supplemented with 10 µM ROCK inhibitor (Y-27632 ...
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bioRxiv - Neuroscience 2023Quote: ... Neural progenitor cells were then dissociated and replated on poly-l-ornithine/fibronectin/laminin-coated plates and maintained in BrainPhys Basal medium (StemCell Technologies) containing B-27 supplement ...
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bioRxiv - Immunology 2023Quote: ... 200-500 μm cystic EBs were selected under a dissecting microscope and transferred onto adherent tissue culture plates (∼2.5 EBs/cm2) for cultivation in Hematopoietic Differentiation (HD) medium (APEL 2, Stem Cell Tech, 05270 ...
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bioRxiv - Cell Biology 2022Quote: ... Dissociated cells were plated on a Matrigel or Culturex coated culture plate and cultured in 10 uM Y27632 (72304, StemCell Technologies) containing mTeSR1 one day before differentiation ...
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bioRxiv - Genomics 2023Quote: ... human iPSCs were plated at cell density of 3.5×105 cells/well on Matrigel coated 12-well-plates using mTeSR™ medium supplemented with 5µM ROCK inhibitor (Y-27632, STEMCELL Technologies) for 24 h ...
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bioRxiv - Cancer Biology 2023Quote: The six basal- and luminal-like cell lines were disassociated with 0.25% trypsin and added individually to wells of a 6 well Ultra-low attachment plate in 2 mL Mammocult culturing media (STEMCELL Technologies). Accutase (STEMCELL Technologies ...
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bioRxiv - Bioengineering 2023Quote: ... 2.5 g of Hydrosil silicone components (1:1 wt) (101301; SILADENT) was added into each well of 6-well microwell culture plate (AggreWellTM400; STEMCELL Technologies) followed by centrifugation at 55 x g for 1 min and curing at 60 °C for 1 h ...
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bioRxiv - Molecular Biology 2023Quote: The colony-forming unit (CFU) assay was performed by seeding 500 cells in six-well plates containing MethoCult Enriched (StemCell Technologies) after 4 days of editing ...
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bioRxiv - Cancer Biology 2023Quote: ... 1.1 mL of methocult-cell suspension was plated into a 6-well plate using 16-gauge blunt-end needles (STEMCELL Technologies). After 14 days ...
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bioRxiv - Biophysics 2023Quote: ... hiPSCs were seeded onto Matrigel-coated 6-well tissue culture treated plates and maintained for 4 days with mTeSR1 media (85850, Stemcell Technologies). On day 0 ...
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bioRxiv - Developmental Biology 2024Quote: ... A 1-5 min centrifugation of the well-plate was performed at 200g and cells in suspension were cultured for one day in mTesR Plus (StemCell Technologies) to allow for aggregation and Embryoid Body (EB ...
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bioRxiv - Bioengineering 2023Quote: ... we single cell sorted ATTO550 positive cells for monoclonal expansion in 96-well plates containing their corresponding dTMP-supplemented culture media (hiPSC additionally supplemented with 10% Clone R (StemCell Technologies)) ...
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bioRxiv - Cell Biology 2023Quote: ... For time first division Single cell LIN-CD34+ CD38- CD9+ or CD9- cells sorted in to the round bottomed 96 well plates and culture in SFEM (StemCell Technologies), supplemented with SCF (10ng/ml) ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells were cultured for 72 hours in 96-well plates (2X105-5X105 cells per well) containing StemPro (Stem Cell Technologies) media supplemented with human IL-6 ...
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bioRxiv - Cancer Biology 2024Quote: ... non-TC-treated 96-well U-bottom plates were treated with 100 μL of anti-adherence rinsing solution (StemCell Technologies 07010) for 1 h ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were resuspended and 50,000-100,000 cells were transferred into fresh matrix-coated plates with mTeSR Plus media containing 10 μM Y-27632 (ROCK inhibitor, STEMCELL Technologies). Fresh media without ROCK inhibitor was fed the next ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were gently resuspended in media and transferred into 6-well plates in mTeSR Plus media containing CloneR2 reagent (STEMCELL Technologies) for AAVS1 targeting ...
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bioRxiv - Immunology 2020Quote: ... the EasySep Kit (StemCell Technologies, cat. 18000) was used according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... NK-cell Enrichment Kit (Stem Cell Technologies) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... the ALDEFLUOR kit was used (StemCell Technologies), following the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... using memory CD8+ isolation kit (StemCell Technologies). T cells were harvested and washed three times with Opti-MEM (LifeTechnologies) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... STEMdiff Cerebral Organoid Kit (StemCell Technologies, 08570) was used for organoid culture ...
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bioRxiv - Immunology 2022Quote: ... and then the ALDEFLUOR kit (Stemcell Technologies) protocol was followed ...
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bioRxiv - Developmental Biology 2023Quote: ... The hPSC Genetic Analysis Kit (Stemcell Technologies) was used following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... StemLight Pluripotency Antibody Kit (Stem Cell Technologies) was used for pluripotency testing ...
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bioRxiv - Cell Biology 2024Quote: ... and c-Kit/CD117 antibodies (STEMCELL Technologies) for 30 min ...
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bioRxiv - Immunology 2021Quote: ... T cells from were then activated with Miltenyi MACS GMP T cell TransAct™ CD3/CD28 beads and seeded 1×10^6 T cells/ml in serum-free StemCell Immunocult™-XF media (StemCell Technologies, Vancouver, Canada) with clinical grade 20U/ml human IL-2 (Novartis)
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bioRxiv - Immunology 2020Quote: Whole blood was collected into EDTA tubes and then diluted with an equal volume of phosphate-buffered saline (PBS) + 2% fetal bovine serum (FBS) and layered over Ficoll using SepMate™-50 tubes (STEMCELL Technologies Inc., Vancouver, Canada). Cells were centrifuged at 1200×g for 10 min at room temperature ...
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bioRxiv - Cell Biology 2019Quote: Whole-brain cerebral organoids were generated with a commercial kit (STEMdiff Cerebral Organoid Kit, STEMCELL Technologies, Catalogue Number 08570), which has been adopted and optimized based on previously published protocol50 ...
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bioRxiv - Immunology 2020Quote: Total or naïve mouse CD4+ T cells were isolated from the spleen or mesenteric LNs by negative selection with the EasySep Mouse CD4+ T cell enrichment kit or the EasySep Mouse naïve CD4+ T cell enrichment kit according to the manufacturer’s protocol (Stemcell Technologies). In certain cases ...
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bioRxiv - Immunology 2024Quote: ... monocytes were enriched from cryopreserved PBMCs using EasySep™ Human Monocyte Enrichment Kit without CD16 Depletion Kit (STEMCELL technologies). Enriched monocytes were stimulated with 100ng/ml LPS for 4h at 37 °C before staining ...
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bioRxiv - Neuroscience 2021Quote: ... at 5% CO2/95% air condition at 37°C and were passaged using ReLeSR™ (Stem Cell Technologies). Karyotype analysis of G-banded metaphase chromosomes has been performed to confirm chromosomal integrity ...
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bioRxiv - Cancer Biology 2020Quote: ... and passaged by dissociating to single cells (5-minute treatment with 1X Accutase at 37 °C; STEMCELL Technologies) and resuspending at a concentration of 100k-300k cells/mL.
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bioRxiv - Developmental Biology 2020Quote: ... and pluripotent stem cells were passaged every 5 to 7 days in the ReLeSR medium (Stem Cell Technologies). Pluripotent iPSC and hESC were differentiated into retinal organoids as previously described (9 and Fig S1 ...
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bioRxiv - Neuroscience 2022Quote: ... 5 min) and plated at a density of 90,000 cells/mL in STEMdiff Neural Induction Medium (StemCell Technologies) with SMADi supplement (StemCell Technologies ...
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bioRxiv - Neuroscience 2022Quote: ... on Matrigel-coated culture dishes at 37°C and 5% CO2 and passaged using ReLeSR (Stem Cell technologies). CRX-mCHERRY hiPSCs were grown on vitronectin (VTN-N ...
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bioRxiv - Cell Biology 2022Quote: ... at 37°C for 5 min and resuspended in E8 plus 10 µM Y-27632 (Stem Cell Technologies) and seeded into AggreWell 800 plates (StemCell Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were subjected to red cell lysis in 5 ml ammonium chloride lysis buffer solution (Stem Cell Technologies) on ice for 10 minutes ...
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Efficient Methods for Target Gene Manipulation in Haematopoietic Stem Cell Derived Human NeutrophilsbioRxiv - Cell Biology 2023Quote: ... Cells were plated at a minimum density of 5 × 105 cells/mL in SFEM II medium (StemCell Technologies) with StemSpanTM CD34+ Expansion Supplement (StemCell Technologies) ...
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bioRxiv - Developmental Biology 2023Quote: ... and kept on Rho-associated protein kinase (ROCK) inhibitor Y-27632 (final concentration 5 μM, StemCell Technologies, #72302) for one day ...
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bioRxiv - Cell Biology 2024Quote: ... at 37°C for 5 min and resuspended in E8 plus 10 µM Y-27632 (Stem Cell Technologies) and seeded into AggreWell 800 plates (StemCell Technologies ...