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Citations for Stemcell Technologies :
701 - 750 of 1346 citations for 7 Chloro 3 methyl 3H 1 2 3 triazolo 4 5 d pyrimidine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... the cell pellet from one 60 mm dish was resuspended in 2 mL mFreSR medium (Stemcell Technologies) to freeze two vials of the cells by putting them in an isopropanol freezing container at –80°C overnight before transferring them into liquid nitrogen.
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bioRxiv - Cell Biology 2020Quote: ... domes diluted 1:1 with advanced DMEM/F12 media (Stemcell Technologies, UK), with 50 μl domes per well ...
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bioRxiv - Physiology 2022Quote: ... then medium changed to 1:1 cFAD+RI:Pneumacult ALI (Stemcell Technologies, UK) for 2 days to ensure monolayers were 100% confluent ...
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bioRxiv - Cancer Biology 2021Quote: Cells were plated on uncoated low-adhesion 96-well plates in a two-fold dilution series spanning from 2000 down to 4 cells per well in NeuroCult NS-A media (StemCell Technologies, #05751) containing doxycycline at 2 μg/mL ...
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bioRxiv - Molecular Biology 2022Quote: ... The medium was changed daily and the cells were passaged every 4–8 days depending on confluency using Gentle Cell Dissociation Reagent (Stem Cell Technologies). 10 μM of ROCKi (Stem Cell Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... washed with phosphate buffered saline (PBS) and incubated overnight at 4 °C with a mouse monoclonal anti-CD3 (clone UCHT1, StemCell Technologies, #60011) 10 μg/mL in PBS ...
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bioRxiv - Cancer Biology 2020Quote: ... 2×10^4 cells from bone marrow of xenotransplanted mice were transferred to 2ml of MethoCult H4034 optimum methylcellulose medium (Stem Cell Technologies) and plated onto two 35mm dishes ...
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bioRxiv - Cell Biology 2020Quote: ... the imaging chamber was incubated overnight at 4° C with 10 μg/mL of a mouse monoclonal anti-CD3 (clone UCHT1, StemCell Technologies, #60011) in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... washed with phosphate buffered saline (PBS) and incubated overnight at 4° C with a mouse monoclonal anti-CD3 antibody (clone UCHT1, StemCell Technologies, #60011) or a mouse monoclonal anti-HA antibody (clone 16B12 ...
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bioRxiv - Bioengineering 2023Quote: IMR90-4 iPSCs (WiCell, Madison, WI) were cultured on Matrigel-coated 6-well plates and supplemented daily with E8 medium (Stem Cell Technologies) as described (81) ...
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bioRxiv - Genetics 2024Quote: ... cells were harvested by centrifugation at 380 x g for 10 min at 4°C and resuspended in EasySepT Buffer (Stem Cell Technologies). HSPCs were purified by negative selection using the EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (Stem Cell Technologies) ...
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Pluripotent stem cell SOX9 and INS reporters facilitate differentiation into insulin-producing cellsbioRxiv - Developmental Biology 2021Quote: ... centrifuged at 50 x g for 5 min and subsequently dissociated by incubation with gentle cell dissociation solution (StemCell Technologies) for 15 min and additional T/E for 10 min ...
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bioRxiv - Bioengineering 2021Quote: ... The microcapsules carrying HUES-8 cells were collected into 15 mL tube filled with 5 mL E8 medium (STEMCELL Technologies), then distributed into a 6 well-plate at density of 2000 capsules/well and incubated at 37 □ with 5% CO2 ...
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bioRxiv - Cell Biology 2022Quote: ... They were patterned into definitive endoderm using the STEMdiff Definitive Endoderm Kit per manufacturer’s instructions over 5 days (StemCell Technologies). Cells were passaged on day 5 of differentiation using GCDR ...
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bioRxiv - Cancer Biology 2022Quote: ... freshly dissected prostate tissues were dissociated in DMEM containing 5% FBS and 1x collagenase/hyaluronidase (STEMCELL Technologies, Vancouver, BC, Canada), followed by trypsinization with 0.25% trypsin-EDTA (Gibco ...
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bioRxiv - Cell Biology 2019Quote: ... mice were injected with 5-15×105 NK cells purified using the NK cell enrichment negative selection kit (STEMCELL Technologies).
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bioRxiv - Neuroscience 2022Quote: hiPSCs were cultured at 37ºC and 5% CO2 in a humidified incubator (BINDER C150) in mTeSR1 medium (STEMCELL Technologies, #85850) on Matrigel (Corning ...
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bioRxiv - Genomics 2021Quote: ... The medium was changed every day and the cells were passed every 5-6 days using ReLeSR (Stem Cell technologies). 24h prior transfection ...
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bioRxiv - Cancer Biology 2022Quote: ... containing 2% FBS and the tissue was further dissociated by treatment with dispase (5 mg/mL)/DNaseI (0.1 mg/mL) solution (STEMCELL Technologies). The cell suspension was filtered using a 40 µm cell strainer (Falcon ...
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bioRxiv - Neuroscience 2022Quote: ... NPCs were plated at 1.25×5 cells/cm2 on Matrigel hESC-qualified Matrix-coated wells and maintained in Neural Progenitor medium (StemCell Technologies) with daily complete media change ...
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bioRxiv - Physiology 2022Quote: ... Cells were cultured in mTESR1 media at 37°C at 5% CO2 and passaged using accutase (Stemcell Technologies, Cat. # 07920) and media supplemented with Y-27632 2HCl (ROCK ...
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bioRxiv - Immunology 2023Quote: ... The cells were incubated at 37°C and 5% v/v CO2 until confluency in PneumaCultTM-ExPlus media (STEMCELL Technologies) for 4-7 days before being switched to ALI culture conditions by removing the apical media and feeding the basal side with PneumaCultTM ALI medium (STEMCELL Technologies) ...
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bioRxiv - Cell Biology 2022Quote: ... GINS precursors were dissociated by 5-10 min TrypLE treatment and aggregated (typically 2.0-2.4 million cells/well) in AggreWell400 (STEMCELL Technologies, 34450) using the manufacturer’s recommended protocol ...
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bioRxiv - Genetics 2022Quote: ... HSPCs were plated at a concentration of 5×102 cells/mL in a methylcellulose-containing medium (GFH4435, Stem Cell Technologies) under conditions supporting erythroid and granulo-monocytic differentiation ...
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bioRxiv - Cell Biology 2023Quote: ... the monolayers were washed twice with PBS then incubated in dispase solution (5 units/mL in HBSS, Stemcell Technologies #07913) for 80 minutes at 37 °C to detach the monolayers from the plate ...
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bioRxiv - Bioengineering 2023Quote: High cell density (4×107 cells/mL) hPSC strips containing 1.5mg/mL collagen with 5% Geltrex were collected in an Anti-Adherence Rinsing Solution (STEMCELL Technologies) treated 60mm Petri dish ...
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bioRxiv - Immunology 2024Quote: ... mice and bone marrow progenitors were plated on non- tissue treated dishes and differentiated into BMDM during a 6-day culture in complete Dulbecco’s modified Eagle’s medium (DMEM) containing 5% FBS and supplemented with 60 ng/ml recombinant murine M-CSF (Stemcell Technologies) in a water saturated atmosphere at 37°C ...
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bioRxiv - Cell Biology 2021Quote: Hepatostellate organoids at day 17 were treated with dissociation buffer, (1:1:1 mixture of 0.25% Trypsin-EDTA, Accutase (Stem cell Tech.), and Collagenase IV (1 mg/mL ...
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bioRxiv - Immunology 2022Quote: ... Remaining whole blood was diluted 1:1 with RPMI + 1% FBS + 2mM L-Glutamine + 100 U Penicillin/Streptomycin and layered onto SEPMATE tubes (STEMCELL Technologies) containing lymphoprep gradient (STEMCELL Technologies) ...
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bioRxiv - Developmental Biology 2022Quote: ... medium was replaced by 1 ml of a 1:1 mixture of UPC medium and reprogramming medium TeSR-E7 (STEMCELL Technologies). From day 4 to day 18-20 ...
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bioRxiv - Immunology 2021Quote: ... Remaining whole blood was diluted 1:1 with RPMI + 1% FBS + 2mM L-Glutamine + 100 U Penicillin/Streptomycin and layered onto SEPMATE tubes (STEMCELL Technologies) containing lymphoprep gradient (STEMCELL Technologies) ...
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bioRxiv - Immunology 2023Quote: ... Remaining whole blood was diluted 1:1 with RPMI + 1% FBS + 2mM L-Glutamine + 100 U Penicillin/Streptomycin (R1) and layered onto SEPMATE tubes (STEMCELL Technologies) containing lymphoprep gradient (STEMCELL Technologies) ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 ng/ml H-PDGF-BB (Shenandoah #100-16) and 2 μg/ml heparin (Stem Cell Technologies #07980). All cells were grown in a standard humidified cell culture incubator at 37 °C and 5% CO2 ...
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bioRxiv - Neuroscience 2020Quote: ... and further expanded in Neurocult NSA with proliferation supplement (StemCell Technologies; 10ng/ml rhFGF-2, 20ng/ml rhEGF) and incubated at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... PBMCs separation was performed within 2 h of blood withdrawal using SepMate™ tubes (StemCell Technologies, BC, Canada) as per manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... T cells were cryopreserved at 2×107 cells per mL in CryoStor CS10 cryopreservation media (Stem Cell Technologies) until use ...
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bioRxiv - Immunology 2023Quote: ... was used at 2 µg/mL.11,14 Immunocult-ACF Human B Cell Expansion Supplement (BAC, Stem Cell Technologies) was used at 1:50 dilution per manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: ... rosette selection was performed by incubating for 2 hours with Neural Rosette Selection Reagent (Stem Cell Technologies, 05832), forcefully dislodging with warm DMEM/F12 ...
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bioRxiv - Synthetic Biology 2023Quote: ... followed by 2X dilution in Dulbecco’s Phosphate Buffered Saline with 2% Fetal Bovine Serum (Stemcell Technologies, Cat.#07905), and finally ...
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bioRxiv - Cancer Biology 2023Quote: Viable BM cells (2×104) were seeded in 1.1 mL Mouse Methylcellulose Complete Media (M3434; Stem Cell Technologies) in uncoated 6 well plates (3471 ...
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bioRxiv - Microbiology 2023Quote: ... mixed 1:1 with PneumaCult™-Ex Plus Medium (STEMCELL Technologies cat# 16237380). All cell lines were discarded after passage 15 ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 1:10 dilution of 1 mg/ml DNase I (STEMCELL Technologies), triturated vigorously for 2 min ...
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bioRxiv - Immunology 2020Quote: ... Remaining whole blood was diluted 1:1 with 1% RPMI (Table S4) and layered into a SEPMATE tube (STEMCELL Technologies, Cat#85450) pre-loaded with lymphoprep (Alere Technologies ...
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bioRxiv - Developmental Biology 2022Quote: ... the media was changed to pre-warmed 37°C mesoderm / Endothelial “priming” media (8 uM CHIR99021 +25 ng/ml BMP4, 1× Glutamax, 1× N2, 1× B27, Neurobasal, DMEM/F12, Stem Cell Technologies). One well of seeded cells was left untreated as negative control in StemFlex (data not shown) ...
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bioRxiv - Bioengineering 2024Quote: ... Dissociation was performed by incubation in dissociation solution (1:1 dilution of 2.5% Trypsin (GibcoTM, Cat#: 15090046) and 1 U/ml Dispase (Stemcell Technologies, Cat#: 15569185)) for 10 min under gentle shaking and occasional gentle pipetting at 28°C ...
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bioRxiv - Genomics 2019Quote: ... and 8 weeks old adult mammary glands (#4 gland only) were dissected and pooled from multiple animals into dissection media (Epicult-B Basal medium (Stem Cell Technologies #05610) supplemented with 5% FBS ...
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bioRxiv - Bioengineering 2023Quote: ... IMR90-4 iPSCs (WiCell, WB65317) between passages p35∼45 were maintained on Matrigel-coated dishes in mTeSR Plus medium (STEMCELL Technologies, 100-0276) and dissociated using ReLeSR (STEMCELL Technologies ...
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bioRxiv - Bioengineering 2023Quote: ... Thawed PMBCs from a vial containing around 1 × 107 cells were pre-cultured in one well of low attachment 6-well plate including 4 mL of StemSpan-AOF (Cat#ST100-0130, STEMCELL Technologies, Vancouver, Canada) supplemented with recombinant human IL-6 (100 ng/mL) ...
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bioRxiv - Immunology 2023Quote: ... were cultured for 4 days post-split in IntestiCult™ Organoid Growth Medium and then switched IntestiCult™ Organoid Differentiation Medium (STEMCell Technologies) for 72 hours to promote epithelial stem cell differentiation ...
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bioRxiv - Neuroscience 2021Quote: ... Suspension was mixed with 250 ul of 50% Iodixanol solution (25 mM KCl, 5 mM MgCl2, 10 mM Tris pH 8.0, 50% Iodixanol (60% stock from STEMCELL Technologies, 7820), 1x protease inhibitor (Roche ...