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Citations for Stemcell Technologies :
701 - 750 of 785 citations for 5 Bromo 2 Tert Butyldimethylsilyl 4 Methylthiazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2024Quote: ... 10,000 cells were plated per 12-well plate well onto 5% biolaminin 521 LN and differentiation started 6 hrs post plating in TeSR-E6 (Stem Cell Technologies, 05946) with CHIR99021 (Tocris ...
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bioRxiv - Bioengineering 2024Quote: ... These cells were maintained in a 5% CO2 incubator at 37°C and were fed every 24 to 48 hours with mTeSR Plus medium (StemCell Technologies, 100-0276). The iPSCs were passaged using the StemPro EZPassage tool (Life Technologies ...
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Genetically engineered microglia-like cells have therapeutic potential for neurodegenerative diseasebioRxiv - Neuroscience 2021Quote: ... Lineage negative cells were transduced with a lentiviral vector in a cell incubator at 2 ×106 cells/mL in either StemSpan SFEM II (STEMCELL Technologies) or StemMACS HSC Expansion Media (Miltenyi Biotec ...
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bioRxiv - Bioengineering 2021Quote: ... microcapsules carrying HUES-8 cells were immersed in E8 medium for 1 h immediately after encapsulation and then transferred into E6 medium (without FGF-2 and TGF-β1, STEMCELL Technologies) for subsequent cultivation (2000 microcapsules per well) ...
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bioRxiv - Cancer Biology 2021Quote: ... 4T1 tumors were extracted and minced with sterile razor blades and incubated for 2 h in the presence of Collagenase (1,000 units per sample) in sterile Epicult media (Stem Cell Technology). Cells were washed with sterile filtered PBS supplemented with 1% bovine serum albumin (PBS-BSA 1% ...
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bioRxiv - Neuroscience 2022Quote: ... Single cells were plated (2 x 106/well) in Matrigel-coated 6-well plate in mTeSR1 plus growth medium (STEMCELL Technologies), supplemented with 10 mM Y-27632 ROCK inhibitor (Calbiochem ...
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bioRxiv - Developmental Biology 2022Quote: ... hESCs were plated onto Matrigel at a density of 2×105 cells/cm2 in E8 medium containing 10 µM ROCK inhibitor (STEMCELL Technologies) and cultured overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... Tissues were cut into 1-2 mm pieces and subsequently incubated in serum-free medium containing collagenase/hyaluronidase (Stem Cell Technologies) at 37 ºC ...
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bioRxiv - Bioengineering 2020Quote: ... On days 8-15+ cell culture media was exchanged every 2 days with 15 mL of Cardiomyocyte Maintenance Medium (STEMCELL Technologies). On day 30 ...
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bioRxiv - Cell Biology 2022Quote: CFU assays in PHZ-treated and retrovirally-infected spleen progenitors were conducted using spleen suspensions at 2 × 104 cells/mL mixed with Methocult M3434 (STEMCELL Technologies) containing Epo ...
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Stem cell-derived macrophages as a new platform for studying host-pathogen interactions in livestockbioRxiv - Immunology 2021Quote: ... Cells were plated at a density of 2-3×103/cm2 in pEPSCM medium27 containing the Rho-associated coiled kinase (ROCK) inhibitor Y-27632 (5μM, Stemcell Technologies, #72304). Cells were fed the following day with pEPSCM without Y-27632 then fed daily ...
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bioRxiv - Immunology 2022Quote: ... FACS sorting was performed on an Influx-2 on pre-enriched CD4+ T cells (enrichment using an EasySep CD4+ T cell isolation kit (StemCell Technologies)) ...
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bioRxiv - Neuroscience 2022Quote: ... human PDGF-AA (10 ng/ml) and PDGF-BB (10 ng/ml; Shenandoah) and heparin (2 ng/ml; Stem Cell Technologies).
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bioRxiv - Developmental Biology 2022Quote: ... cells were collected by Trypsin and half of the well equivalent was plated in 2 ml of methylcellulose medium (Human enriched H4435, Stemcell Technologies). Cells were incubated in the assay for 14 days and then scored.
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bioRxiv - Cell Biology 2022Quote: ... Cell pellets (containing 2 × 106) were cultured for 21 days in MesenCult™-ACF Chondrogenic Differentiation Kit (STEMCELL TECHNOLOGIES, Inc, Canada) to induce differentiation ...
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bioRxiv - Synthetic Biology 2022Quote: ... Two days post-transfection, the cells were replated in a 6 well (Falcon, 353224) and selected in 2 μg/ml Puromycin (STEMCELL Technologies). Every two days ...
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bioRxiv - Developmental Biology 2022Quote: ... From day 7 - 9 of differentiation cells were cultured in cSFDM containing 2 μM Retinoic acid and 0.5 μg/ml purmorphamine (Stemcell Technologies, #72202), and then subsequently in cSFDM with 2 μM Retinoic acid until day 11-13 of differentiation ...
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bioRxiv - Neuroscience 2024Quote: ... SH-SY5Y cells were counted and seeded at a density of 10.4 × 103 cells/cm2 in differentiation media (Neurobasal medium, 2% B27 supplement, 1% GlutaMAX, and 10 μM of all-trans retinoic acid [STEMCELL Technologies]). Differentiation media was replaced every other day until day 9 post-seeding ...
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bioRxiv - Neuroscience 2024Quote: ... media was removed and neural induction media (NIM; Supplementary Table 2) supplemented with small molecules 10 µM SB431542 (StemCell Technologies, #72146) and 0.1 µM LDN193189 (StemCell Technologies ...
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bioRxiv - Developmental Biology 2024Quote: ... SSI and SSII media are parts of a 2-step suspension culture system with a base medium consisting of StemSpan Serum-Free Expansion Medium II (StemCell technologies), 2 mM of L-glutamine ...
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bioRxiv - Cell Biology 2024Quote: Whole blood was collected from 12M and 20M SM/J and BL6 mice via intracardiac puncture and diluted into an equal volume of Dulbecco’s PBS + 2% FBS (StemCell Technologies, 07905). According to the manufacturer’s specifications ...
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bioRxiv - Neuroscience 2023Quote: ... 550,000 neurons were seeded in 6 well plates coated with 1 mg/mL polyLysine were cultured in conditioned Neurobasal medium supplemented with 2 mM L-glutamine and NeuroCult SM1 Neuronal supplement (STEMCELL Technologies) half renewed every 3 days with 3.4mM AraC ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 x 104 sorted cells were plated in triplicate in serum-free media containing StemSpan™ Megakaryocyte Expansion Supplement (STEMCELL Technologies) for 10-12 days ...
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bioRxiv - Immunology 2023Quote: ... 200-500 μm cystic EBs were selected under a dissecting microscope and transferred onto adherent tissue culture plates (∼2.5 EBs/cm2) for cultivation in Hematopoietic Differentiation (HD) medium (APEL 2, Stem Cell Tech, 05270 ...
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bioRxiv - Cancer Biology 2023Quote: ... co-cultures were counted and recultured as 1 x 106 cells/ml in fresh medium containing 20 U/ml recombinant human IL-2 (StemCell Technologies) in the presence of soquelitinib or DMSO for additional 5 days ...
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bioRxiv - Cancer Biology 2023Quote: The six basal- and luminal-like cell lines were disassociated with 0.25% trypsin and added individually to wells of a 6 well Ultra-low attachment plate in 2 mL Mammocult culturing media (STEMCELL Technologies). Accutase (STEMCELL Technologies ...
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bioRxiv - Bioengineering 2023Quote: ... The PBMC pellet was resuspended in PBS supplemented with 2% FBS and 1 mM EDTA and monocyte isolation was performed using the EasySepTM human monocyte isolation kit (Stemcell Technologies) according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... bone marrow aspirates were diluted 1:1 with PBS (+ 2% FBS) and layered on a Lymphoprep gradient using SepMate tubes (STEMCELL Technologies). After centrifugation ...
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bioRxiv - Cancer Biology 2023Quote: ... 1.1ml of the mixture (2 x 104 BM cells and 2 x 105 spleen cells) was plated in triplicate into 6-well dishes (SmartDishTM, STEMCELL Technology). After incubation for 10 days ...
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bioRxiv - Cell Biology 2024Quote: ... IL-11 and SCF cultures:2 Equal volume of medium was added to each well to a final concentration of 10% FBS (STEMCELL Technologies), 1% penicillin/streptomycin (Sigma-Aldrich) ...
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bioRxiv - Immunology 2024Quote: ... were irradiated with 2 Gy by 177Lu diluted in 3 mL of ImmunoCult-XF T cell Expansion Medium (cat. no. 10981; STEMCELL Technologies) supplemented with 50 IU/mL of IL-2 over 3 days and then examined by flow cytometry for viability as previously described 25.
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bioRxiv - Cancer Biology 2024Quote: Tissue samples of ovarian tumours were cut into ∼1-2 mm3 pieces using sterile scalpels and digested with collagenase type IV (STEMCELL Technologies) for 2 hours at 37°C on a rotator ...
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bioRxiv - Synthetic Biology 2021Quote: ... in borate buffer for primary culture cells or Poly-D-Lysine for cells from an iPSC background before being coated with either 10 µg/ml mouse laminin or 5 µg/ml human 521 Laminin (Stemcell Technologies Australia, Melbourne, Australia) respectively to facilitate cell adhesion.
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bioRxiv - Microbiology 2021Quote: ... the isolated intestinal tissue was cut into ∼3-to 5-mm pieces and incubated in Gentle Cell Dissociation Reagent (Stem Cell Technologies, Vancouver, Canada). After removal of the Gentle Cell Dissociation Reagent ...
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bioRxiv - Developmental Biology 2022Quote: ... Roughly separated tubules were incubated in collagenase type IV in DMEM (5 mL for 1 testis pair, 1 mg/mL, STEMCELL Technologies Inc., Cat# 07909) at 37 °C while rotating ...
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bioRxiv - Developmental Biology 2024Quote: ... and the organoid pellet was resuspended in 5-10 mL pre-cooled basal DMEM/F-12 medium containing 15 mM HEPES (Stem Cell Technologies, cat.# 36254) using a 10 mL pipette pre-wetted with 1% (vol/vol ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were kept in a 37°C tissue culture incubator with 5% CO2 and lines were maintained in mTeSR Plus cultured media (Stemcell Technologies Cat# 100-1130). Stem cells underwent directed differentiation into definitive endoderm over a 3-day treatment using Activin A (100ng/mL ...
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bioRxiv - Genomics 2021Quote: ... after which cells were replated in E8 containing p160-Rho-associated coiled-coil kinase (ROCK) inhibitor Y-27632 (2 μM; Stemcell Tech, 72307).
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bioRxiv - Neuroscience 2020Quote: ... Medium was refreshed every 2-3 days and cells were passaged twice a week using an enzyme-free dissociation reagent (ReLeSR, Stem Cell Technologies).
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bioRxiv - Cancer Biology 2022Quote: 2,500 cells were seeded into each well of an ultra-low attached 6-well plate in 2 mL complete MammoCultTM Medium (Stem Cell Technologies) based upon the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... 1 or 2 EBs were transferred to a well of a 24-well ultra-low attachment plate containing Induction Medium (Stem Cell Technologies). The EBs were kept in the induction Medium for 2 days and next they were transferred into Matrigel (Corning ...
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bioRxiv - Neuroscience 2022Quote: ... IPSCs were then collected and spun down at 500rpm for 1-2 minutes and resuspended in 2mL of mTeSR Plus media with 20 µM ROCK inhibitor Y-27632 (Stemcell Technologies # 72304). Matrigel ...
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bioRxiv - Neuroscience 2021Quote: ... Medium was refreshed every 2-3 days and hiPSCs were passaged twice per week using an enzyme-free reagent (ReLeSR, Stem Cell Technologies).
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bioRxiv - Cancer Biology 2021Quote: ... All GSC lines and human neural stem cells were maintained as neurospheres using ultra low-adherence culture dishes and dissociated 1-2 times per week with Accutase (StemCell Technologies 07922). Primary human astrocytes were cultured adherently on plates coated with Geltrex (Life Technologies A1413202) ...
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bioRxiv - Bioengineering 2022Quote: ... were either coated with 70 µL of 2% agarose in water or PBS or treated for 20 min with 100 µL of anti-adherence rinsing solution (StemCell Technologies 07010). The wells were washed with PBS ...
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bioRxiv - Immunology 2021Quote: Whole blood was collected in heparin containing vacuutubes and 12μL of 0.5M EDTA with 1mL of PBS+2% FBS and 50μL of RBC of EasySep RBC Depletion spheres (EasySep ™ RBC Depletion Reagent: 18170, Stem Cell Technologies) were added ...
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bioRxiv - Neuroscience 2022Quote: ... human PDGF-AA (10 ng ml−1) and PDGF-BB (10 ng ml−1; Shenandoah) and heparin (2 ng ml−1; Stem Cell Technologies). For all patient-derived cultures ...
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bioRxiv - Immunology 2022Quote: ... blood was resuspended in PBS/2%FBS/EDTA and separated using Ficoll-Paque Plus (Cytiva, 17144002) density gradient centrifugation in SepMate50 tubes (StemCell Technologies, 85450), followed by two washes in PBS/2%FBS/EDTA ...
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bioRxiv - Neuroscience 2024Quote: ... hESC colonies are passaged and small clusters are plated onto a 6 well plate at a density of ∼ 1-2 x 104 cells/well in TeSR-E8 media (StemCell Technologies, #5990). Following 24 hrs ...
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bioRxiv - Bioengineering 2023Quote: ... Stages 1 and 2 used basal media comprised of 1:1 mixture RPMI and DMEM F-12 supplemented with BSA and SM1 (Stem Cell Technology) or B27 (ThermoFisher ...