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Citations for Stemcell Technologies :
651 - 700 of 1054 citations for Recombinant Human DMBT1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... and in the presence of the commercially available medium IntestiCult™ Organoid Growth Medium (Human) (STEMCELL Technologies, 06010), supplemented with the antibiotic primocin (Invivogen ...
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bioRxiv - Cancer Biology 2021Quote: ... and BT260 cells were cultured in NeuroCult NS-A Basal Medium (Human) with Proliferation Supplement (StemCell Technologies 05750) supplemented with EGF (20 ng/mL) ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T cells were obtained by negative selection using the EasySep Human CD4+ Enrichment kit (Stem Cell Technologies), and stimulated with anti-CD3-coated magnetic beads (Dynabeads M-450 tosyl-activated ...
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bioRxiv - Immunology 2021Quote: ... T cells were stimulated with ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator (Stemcell Technologies cat 10990) with 6.25μl per ml culture media at 2e6cells/ml ...
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bioRxiv - Immunology 2022Quote: ... eNK cells added to T cell cultures were removed (EasySep Human CD56 Positive Selection Kit II, STEMCELL Technologies) prior to analysis ...
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bioRxiv - Immunology 2022Quote: Cells were incubated in 2% FCS in PBS with 1 mM EDTA with human Fc block (STEMCELL Technologies) and stained with Aqua LIVE/DEAD Fixable Dead Cell Stain Kit (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... T cells were polyclonally activated using ImmunoCult Human CD3/CD2/CD28 T Cell Activator (1:100) (Stemcell Technologies).
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bioRxiv - Immunology 2022Quote: ... T cells were polyclonally activated using ImmunoCult Human CD3/CD2/CD28 T Cell Activator (1:100) (Stemcell Technologies). Before activation and at different time points thereafter ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells were enriched by Lymphoprep and RosetteSep Human CD4+ T Cell Enrichment Cocktail (both STEMCELL Technologies). CD4-enriched cells were then CD25-enriched using CD25 MicroBeads II (Miltenyi Biotec) ...
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bioRxiv - Cancer Biology 2024Quote: ... CD3+ T-cell populations were isolated using RosetteSep Human T Cell Enrichment Cocktails (Stemcell Technologies, 15023 and 15022) and EasySep Human CD4 T Cell Iso Kit (Stemcell Technologies ...
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bioRxiv - Immunology 2024Quote: ... Naïve T cells were isolated by EasySep™ Human Naïve Pan T Cell Isolation Kits (STEMCELL Technologies, 17961). Naïve CD8+ T cells were isolated by EasySep™ Human Naïve CD8+ T Cell Isolation Kit (STEMCELL Technologies ...
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bioRxiv - Microbiology 2024Quote: Primary human CD4+ T cells from healthy donors were isolated from leukoreduction chambers after Trima apheresis (STEMCELL Technologies). PBMCs were isolated by Ficoll centrifugation ...
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bioRxiv - Immunology 2023Quote: ... and CD14+ cells were purified using the EasySep™ Human CD14 Positive Selection Kit II (StemCell Technologies, 17858). A total of 1.5×106/well CD14+ monocytes were seeded in a 12-well plate with 50 ng/mL of hM-CSF (Peprotech ...
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bioRxiv - Bioengineering 2024Quote: ... CD34+ cells were isolated using EasySep™ Human CD34 Positive Selection Kit II (STEMCELL Technologies, Vancouver, BC, Canada) as per manufacturer instructions ...
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bioRxiv - Bioengineering 2024Quote: Primary adult blood cells were obtained from healthy human donors as a leukapheresis pack purchased from StemCell Technologies, Inc ...
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bioRxiv - Immunology 2023Quote: ... was used at 2 µg/mL.11,14 Immunocult-ACF Human B Cell Expansion Supplement (BAC, Stem Cell Technologies) was used at 1:50 dilution per manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... naive Treg cells were purified by using EasySep Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit (STEMCELL Technologies). For human cell culture ...
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bioRxiv - Immunology 2023Quote: ... CD4+ T cells were isolated from PBMCs with the EasySep Human CD4+ T cell Enrichment Kit (STEMCELL Technologies). Genomic DNA was isolated from a median of 3.65 million CD4+ T cells (IQR 2.48-4.98 ...
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bioRxiv - Immunology 2023Quote: ... B cells were purified from TMNCs with the EasySep Human CD19 Positive Selection Kit II (Stem Cell Technologies) per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... CD3+ T cells were isolated from PBMCs using EasySep™ Human T Cell Isolation Kit (StemCell Technologies, Taiwan) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... at 2.5 x 106 cells/mL and ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator (STEMCELL Technologies) at 25μL/mL ...
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bioRxiv - Cell Biology 2023Quote: ... Human primed PSCs cultured in mTeSR1 medium on Matrigel were passaged every five days using Dispase (STEMCell Technologies) at 1:10 split ratio.
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bioRxiv - Genomics 2023Quote: ... The human iPSC-derived cardiomyocytes (hiPSC-CMs) were reprogrammed using the STEMdiff™ Cardiomyocyte Differentiation Kit (STEMCELL Technologies) as recommended by the manufacturer ...
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bioRxiv - Immunology 2023Quote: PBMCs were obtained for CD14 positive monocyte isolation using the EasySep Human Monocyte Isolation Kit (Stem cell Technologies), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... and UTSW63 cells were cultured in NeuroCult NS-A Basal Medium (Human) with Proliferation Supplement (StemCell Technologies 05751), supplemented with EGF (20 ng/mL ...
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bioRxiv - Molecular Biology 2023Quote: ... CD34+ cells were isolated from the purified PBMNCs by EasySep Human CD34 positive selection kit II (STEMCELL Technologies) and expanded in HSC expansion media as described earlier (Genovese et al ...
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bioRxiv - Bioengineering 2023Quote: Human primary chondrocyte spheroids were formed by seeding the cells into AggreWellTM400 microwell culture plate (STEMCELL Technologies, Canada) at 300 ...
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bioRxiv - Bioengineering 2023Quote: Monocytes were isolated from healthy donors’ blood using EasySep™ Human Monocyte Isolation Kit (Stemcell technologies, Cambridge, MA) by the MIT monocyte core facility ...
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bioRxiv - Immunology 2023Quote: ... Neutrophils were isolated by immuno-magnetic negative selection using the EasySep Direct human neutrophil isolation kit (StemCell Technologies). A highly pure population of neutrophils was then generated by cell sorting on the basis of size ...
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bioRxiv - Bioengineering 2024Quote: ... Human T cells were isolated from thawed cryostocks using an EasySep CD3+ T cell isolation kit (StemCell Technologies). T cells were cultured at a 1:2 cell:bead ratio with cultured αCD3/αCD28 activator beads (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... Human peripheral blood mononuclear cells (PBMCs) were isolated via density gradient centrifugation (Lymphoprep, StemCell Technologies Inc, cat#07801). PBMCs were resuspended in cryopreservation media (90% FBS + 10% DMSO ...
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bioRxiv - Cell Biology 2024Quote: The CD34+ HSC cells were isolated using Human Cord Blood CD34 Positive Selection Kit II (Stemcell Technologies, #17896) as per the supplier instruction ...
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bioRxiv - Neuroscience 2024Quote: ... Human iPSCs from the GIH6C1 background were cultured in mTeSR Plus medium (StemCell Technologies; Cat. No. 100-0276). iPSCs were grown in plates or dishes coated with Growth Factor Reduced ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by CD34 magnetic bead-based enrichment using the EasySep human CD34 positive selection kit II (StemCell technologies). We found this enrichment strategy to be simple and reproducible and chose it for several reasons ...
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bioRxiv - Molecular Biology 2024Quote: Multiple aliquots of frozen Human Peripheral Blood Mononuclear Cells were purchased from Stem Cell Technologies (Cat No 70025.2). PMBCs were thawed in complete RPMI 1640 Medium (Thermo Fisher Scientific Cat No 11875093) ...
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bioRxiv - Synthetic Biology 2021Quote: Primary adult blood cells were obtained from anonymous healthy human donors as a leukapheresis pack purchased from StemCell Technologies, Inc ...
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bioRxiv - Systems Biology 2020Quote: ... CD66b and glycophorin A were depleted from PBMCs using magnetic sorting (Human Pan-DC Pre-Enrichment Kit, StemCell Technologies). pDCs were then sorted on a FACS Vantage instrument (BD Biosciences) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Pluripotent human stem cells were grown in the absence of MEF for four passages in mTeSR1 (StemCell Technologies, 05850) and seeded on Geltrex (invitrogen ...
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bioRxiv - Neuroscience 2019Quote: Human iPSCs at low passage number were cultured in mTeSR medium (STEMCELL Technologies; Vancouver, BC, Canada; Cat. No. 85850) in 10-cm culture dishes (Thermo-Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... CD4+ cells were isolated by negative selection (RosetteSepTM Human CD4+ T cell Enrichment Cocktail, STEMCELL technologies, Cambridge, UK; #15023) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... CD4+ T cells were isolated from PBMCs via the Easysep Human CD4+ T-cell negative enrichment kit (STEMCELL Technologies). The isolated human CD4+ T cells were cultured at 2.5 × 106 cells/ml in complete RPMI medium (cRPMI) ...
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bioRxiv - Immunology 2021Quote: T cells were isolated from PBMCs with EasySep™ Human T cell Isolation Kit per manufacturer’s instructions (StemCell Technologies). Isolated T cells (0.5-1 × 105 per well ...
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bioRxiv - Physiology 2019Quote: ... the colonoids were treated with a 1:1 mix of growth medium and IntestiCult-Human culture medium (StemCell Technologies). Colonoids were then dissociated into small cell aggregates (less than 40μm in size ...
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bioRxiv - Microbiology 2019Quote: ... B cells were isolated from PBMCs through negative enrichment using EasySep Human B cell isolation kit (Stem Cell Technologies) following manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: Peripheral blood mononuclear cells (PBMC) were isolated from fresh human buffy coats using immunomagnetic negative selection (Stem Cell Technologies) and cultured in RPMI-1640 (Gibco ...
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bioRxiv - Immunology 2021Quote: ... ICM T cells were stimulated with ImmunoCult™ Human CD3/CD28 T Cell Activators (25 μl/ml, STEMCELL Technologies). TAC T cells were stimulated with T Cell CD3/CD28 TransAct™ ...
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bioRxiv - Molecular Biology 2021Quote: ... Total CD4+ T cells were separated by negative selection using EasySep Human CD4+ T Cell Enrichment Cocktail (StemCell Technologies). Primary CD4+ T cells were plated at a concentration of 1,5x10 6 cells/mL left overnight for recovery ...
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bioRxiv - Microbiology 2021Quote: ... CD4+ T cells were negatively isolated using the RosetteSep™Human CD4+ T Cell Enrichment Cocktail (Stem Cell Technologies) or the EasySep™ Human Naïve CD4+ T Cell Isolation Kit (Stem Cell Technologies ...
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bioRxiv - Microbiology 2020Quote: Human iPSC (XCL-1)98-derived neural progenitor cells (hNPCs) were purchased commercially (StemCell Technologies, Cat #70901, Lot #17080) and maintained up to ten passages according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Isolation of neutrophils was carried out EasySep™ Direct Human Neutrophil Isolation Kit (Stem Cell Technologies, Vancouver, Canada, 19666), an immunomagnetic negative selection kit ...