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Citations for Stemcell Technologies :
651 - 700 of 1130 citations for Human ADH5 siRNA since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Polyclonal CD8+ T cells were selected using EasySep Human CD8+ T Cell Isolation Kit (STEMCELL Technologies; 17953). Blood monocytes were isolated through negative magnetic sorting (EasySep Human Monocyte Enrichment Kit ...
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bioRxiv - Cancer Biology 2024Quote: ... GBM110 cell line (BTRL, USA) was cultured in NeuroCult NS-A Basal Medium-Human (Stem Cell Technologies) supplemented with NeuroCult NS-A Proliferation Supplements-Human (Stem Cell Technologies) ...
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bioRxiv - Cell Biology 2024Quote: Human dermal fibroblasts (HDFs) were transfected using self-replicating RNA vector ReproRNA™-OKSGM (StemCell Technologies, Canada) according to the manufacturer’s instruction followed by puromycin (ThermoFisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... BMPCs were enriched from bone marrow mononuclear cells using EasySep Human CD138 Positive Selection Kit II (StemCell Technologies) and immediately used for ELISpot or cryopreserved in 10% dimethyl sulfoxide in FBS.
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bioRxiv - Immunology 2021Quote: ... B cells were enriched from PBMC by first using EasySep Human Pan-B cell Enrichment Kit (StemCell Technologies), and then stained with CD20-PB (2H7 ...
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bioRxiv - Immunology 2021Quote: ... NK cells were isolated from whole blood with the EasySepTM Direct Human NK Cell Isolation Kit (StemCell Technologies). The gating strategy for isolation of CD56+CD3- NK cells is shown in Figure S1 (Figure 2 – Supplement 1) ...
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bioRxiv - Developmental Biology 2020Quote: Human embryonic stem cells (hESCs; H9 line) were cultured in mTeSR™1 basal medium (Stem Cell Technologies), in 6-well plates coated with matrigel (Corning Matrigel Matrix hESC-qualified) ...
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bioRxiv - Immunology 2022Quote: ... CD14+ CD16- monocytes were isolated using magnetic negative selection (EasySep Human Monocyte Isolation Kit, StemCell Technologies, Cambridge, Massachusetts) and differentiated into macrophages by culture in RPMI 1640 (Lonza ...
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bioRxiv - Cancer Biology 2020Quote: Frozen human bone marrow mononuclear cells were thawed and washed twice with Iscove’s Modified Dulbecco’s Medium (StemCell Technologies) containing 10% fetal bovine serum (FBS) ...
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bioRxiv - Immunology 2020Quote: ... Monocytes were isolated from PBMC by negative selection using the EasySep™ Human Monocyte Isolation Kit (STEMCELL Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: T and B cell lineage depletion was performed using RosetteSep Human Hematopoietic Progenitor Cell Enrichment Cocktail (Stemcell Technologies) and Ficoll-Paque density gradient centrifugation from routine red cell exchange apheresis performed at Texas Children’s Hospital or Columbia University New York Presbyterian Hospital ...
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bioRxiv - Genetics 2021Quote: ... 100ng/ml human interleukin 6 (hIL-6) (all obtained from EuroBiosciences) and 35nM UM171 molecule (Stem Cell Technologies). Cells were cultured under normoxic conditions ...
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bioRxiv - Cell Biology 2022Quote: ... Both epithelial and stromal cell pellets were then suspended in complete MammoCult human medium (STEMCELL Technologies, Inc., 05620) supplemented with hydrocortisone and heparin as per the manufacturer’s instructions and 1% pen/strep (Sigma ...
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bioRxiv - Developmental Biology 2022Quote: ... 12 w samples were further subjected to EasySep™ Human EpCAM Positive Selection Kit II (STEMCELL Technologies, 18356) to positively select for epithelial cells ...
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bioRxiv - Immunology 2022Quote: ... and CD4 T cells were isolated by immunomagnetic positive selection (EasySep Human CD4 positive selection kit; Stemcell Technologies). Sex and age for these individuals can be found in the metadata corresponding to the RNA-Seq data available from the Sequence Read Archive under accession number PRJNA832136.
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bioRxiv - Immunology 2020Quote: ... prior to CD4 and CD8 T cells isolation using EasySep Human T cell isolation kit (STEMCELL Technology, #17951). Isolated T cells were activated with Dynabeads Human T-Activator CD3/CD28 (ThermoFisher ...
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bioRxiv - Molecular Biology 2022Quote: ... CD34+ cell enrichment was performed using EasySep™ Human CD34 Positive Selection Kit II (StemCell Technologies, Cat 17856) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... positive immunomagnetic selection of CD45+ cells were performed with EasySep Human CD45 Depletion Kit II (StemCell Technologies, Canada) according to the manufacturer instructions (post-isolation purity 96-99%) ...
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Dendritic cells focus CTL responses toward highly conserved and topologically important HIV epitopesbioRxiv - Immunology 2020Quote: ... Bulk T cells were negatively selected using the EasySepTM Human T Cell Enrichment Kit (STEMCELL Technologies, Cambridge, MA), and 7.5 x 105 T cells were added per well to the MDC1 containing wells (MDC1 to T cell ratio = 1:10) ...
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bioRxiv - Biophysics 2020Quote: ... ImmunoCult™ Human CD3/CD28/CD2 T cell activator (short T-Ac) (Stem cell Technologies, Vancouver, BC, Canada) was added to the cell culture medium (25 μL per 1 mL of cell suspension) ...
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bioRxiv - Immunology 2020Quote: ... Naive B cells (CD19+CD27−IgD+) were enriched using Human naive B cell negative selection kit (Stemcell Technologies).
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bioRxiv - Cancer Biology 2020Quote: ... The EasySep™ Direct Human Neutrophil Isolation Kit was used as per standard protocol (StemCell Technologies, cat. 19666). Isolation cocktail containing antibodies and RapidSpheres™ were added to the whole blood sample and immunomagnetic negative selection was performed ...
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bioRxiv - Immunology 2020Quote: Human peripheral blood mononuclear cells were purchased from Stemcell Technologies (#70025) and CD8+ T cells isolated using the EasySep Human CD8+ T Cell Isolation kit (Stemcell Technologies, #17953). Primary human CD8+ T cells were cultured for up to 24 days in ATCC-modified RPMI (Thermo Fisher ...
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bioRxiv - Physiology 2021Quote: The EPO concentrations from 34 serum samples were determined using the Human Erythropoietin ELISA Kit from Stemcell Technologies. Briefly ...
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bioRxiv - Cancer Biology 2021Quote: ... T-cell cultures were fed with fresh 10% RPMI supplemented with human IL2 (20u/ml Stem Cell Technologies) every 2-3 days to maintain 1×106 cells/mL/mm2 ...
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bioRxiv - Cancer Biology 2021Quote: CD14+ monocytes were selected from frozen human PBMC using anti-CD14 microbeads according to manufacturer’s instructions (Stemcell Technologies). The isolated monocytes were counted and resuspended in growth medium (Stemcell Technologies ...
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bioRxiv - Bioengineering 2020Quote: ... and in the presence of the commercially available medium IntestiCult™ Organoid Growth Medium (Human) (STEMCELL Technologies, 06010), supplemented with the antibiotic primocin (Invivogen ...
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bioRxiv - Cancer Biology 2021Quote: ... and BT260 cells were cultured in NeuroCult NS-A Basal Medium (Human) with Proliferation Supplement (StemCell Technologies 05750) supplemented with EGF (20 ng/mL) ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T cells were obtained by negative selection using the EasySep Human CD4+ Enrichment kit (Stem Cell Technologies), and stimulated with anti-CD3-coated magnetic beads (Dynabeads M-450 tosyl-activated ...
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bioRxiv - Immunology 2021Quote: ... T cells were stimulated with ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator (Stemcell Technologies cat 10990) with 6.25μl per ml culture media at 2e6cells/ml ...
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bioRxiv - Immunology 2023Quote: ... naive Treg cells were purified by using EasySep Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit (STEMCELL Technologies). For human cell culture ...
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bioRxiv - Cancer Biology 2023Quote: ... and UTSW63 cells were cultured in NeuroCult NS-A Basal Medium (Human) with Proliferation Supplement (StemCell Technologies 05751), supplemented with EGF (20 ng/mL ...
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bioRxiv - Immunology 2023Quote: ... CD4+ T cells were isolated from PBMCs with the EasySep Human CD4+ T cell Enrichment Kit (STEMCELL Technologies). Genomic DNA was isolated from a median of 3.65 million CD4+ T cells (IQR 2.48-4.98 ...
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bioRxiv - Immunology 2023Quote: ... B cells were purified from TMNCs with the EasySep Human CD19 Positive Selection Kit II (Stem Cell Technologies) per the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... was used at 2 µg/mL.11,14 Immunocult-ACF Human B Cell Expansion Supplement (BAC, Stem Cell Technologies) was used at 1:50 dilution per manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... and CD14+ cells were purified using the EasySep™ Human CD14 Positive Selection Kit II (StemCell Technologies, 17858). A total of 1.5×106/well CD14+ monocytes were seeded in a 12-well plate with 50 ng/mL of hM-CSF (Peprotech ...
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bioRxiv - Bioengineering 2024Quote: Primary adult blood cells were obtained from healthy human donors as a leukapheresis pack purchased from StemCell Technologies, Inc ...
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bioRxiv - Immunology 2024Quote: ... Naïve T cells were isolated by EasySep™ Human Naïve Pan T Cell Isolation Kits (STEMCELL Technologies, 17961). Naïve CD8+ T cells were isolated by EasySep™ Human Naïve CD8+ T Cell Isolation Kit (STEMCELL Technologies ...
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bioRxiv - Microbiology 2024Quote: Primary human CD4+ T cells from healthy donors were isolated from leukoreduction chambers after Trima apheresis (STEMCELL Technologies). PBMCs were isolated by Ficoll centrifugation ...
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bioRxiv - Bioengineering 2024Quote: ... CD34+ cells were isolated using EasySep™ Human CD34 Positive Selection Kit II (STEMCELL Technologies, Vancouver, BC, Canada) as per manufacturer instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... CD34+ cells were isolated from the purified PBMNCs by EasySep Human CD34 positive selection kit II (STEMCELL Technologies) and expanded in HSC expansion media as described earlier (Genovese et al ...
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bioRxiv - Cancer Biology 2024Quote: ... CD3+ T-cell populations were isolated using RosetteSep Human T Cell Enrichment Cocktails (Stemcell Technologies, 15023 and 15022) and EasySep Human CD4 T Cell Iso Kit (Stemcell Technologies ...
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bioRxiv - Bioengineering 2023Quote: Monocytes were isolated from healthy donors’ blood using EasySep™ Human Monocyte Isolation Kit (Stemcell technologies, Cambridge, MA) by the MIT monocyte core facility ...
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bioRxiv - Bioengineering 2023Quote: Human primary chondrocyte spheroids were formed by seeding the cells into AggreWellTM400 microwell culture plate (STEMCELL Technologies, Canada) at 300 ...
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bioRxiv - Immunology 2023Quote: ... Neutrophils were isolated by immuno-magnetic negative selection using the EasySep Direct human neutrophil isolation kit (StemCell Technologies). A highly pure population of neutrophils was then generated by cell sorting on the basis of size ...
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bioRxiv - Immunology 2022Quote: ... eNK cells added to T cell cultures were removed (EasySep Human CD56 Positive Selection Kit II, STEMCELL Technologies) prior to analysis ...
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bioRxiv - Immunology 2022Quote: Cells were incubated in 2% FCS in PBS with 1 mM EDTA with human Fc block (STEMCELL Technologies) and stained with Aqua LIVE/DEAD Fixable Dead Cell Stain Kit (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... T cells were polyclonally activated using ImmunoCult Human CD3/CD2/CD28 T Cell Activator (1:100) (Stemcell Technologies).
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bioRxiv - Immunology 2022Quote: ... T cells were polyclonally activated using ImmunoCult Human CD3/CD2/CD28 T Cell Activator (1:100) (Stemcell Technologies). Before activation and at different time points thereafter ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells were enriched by Lymphoprep and RosetteSep Human CD4+ T Cell Enrichment Cocktail (both STEMCELL Technologies). CD4-enriched cells were then CD25-enriched using CD25 MicroBeads II (Miltenyi Biotec) ...