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Citations for Stemcell Technologies :
651 - 700 of 896 citations for Chloroethane D5 98% 1000 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... the cells were resuspended with 0.5 mL of Complete MesenCultTM-ACF Chondrogenic Differentiation Medium (Stem Cell Technology) supplemented with antibiotics and centrifuged at 300 × g for 10 min to obtain a cell pellet ...
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bioRxiv - Cancer Biology 2023Quote: ... Resuspended cells were then gently mixed with 1.5 mL of reconstituted MethoCult (StemCell Technologies, cat. n. M3134) and aliquot in a single well of an ultra-low attachment 6-well plate (Corning Costar ...
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bioRxiv - Neuroscience 2023Quote: ... the cell pellet from one 60 mm dish was resuspended in 2 mL mFreSR medium (Stemcell Technologies) to freeze two vials of the cells by putting them in an isopropanol freezing container at –80°C overnight before transferring them into liquid nitrogen.
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bioRxiv - Cancer Biology 2023Quote: ... for 5-7 days and embedded into 2 mg/ml collagen type-I (StemCell Technologies, Vancouver, Canada) gels ...
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bioRxiv - Synthetic Biology 2023Quote: ... and ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator (25 µL/mL) (Stemcell Technologies, Cat.#10990) and cultured for 1.5 days at 37°C with 5% CO2 ...
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bioRxiv - Cell Biology 2024Quote: ... seeded 18 hours prior in a 6-well plate with 1 mL of mTESR1 (StemCell Technologies 85850) or MEM medium ...
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bioRxiv - Bioengineering 2024Quote: ... NK cells were frozen at 2.5e6 or 5E6 cells/mL in CryoStor CS10 (STEMCELL Technologies, Cambridge, MA).
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bioRxiv - Bioengineering 2024Quote: ... at a density of 200 cells/well in 1.1 mL of methylcellulose (Methocult H4435 Enriched, StemCell Technologies). This media contains SCF ...
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bioRxiv - Biophysics 2023Quote: ... Cells were cultured on plates incubated with 10 µg/mL Vitronectin XF (Cat. No.07180, STEMCELL Technologies) for at least 1 hour at room temperature prior to passaging ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were then treated with 20 uL/mL of Immunocult Human Treg Differentiation Supplement (StemCell Technologies) containing recombinant human TGF-B1 and all-trans retinoic acid ...
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bioRxiv - Cell Biology 2024Quote: ... or 1:20 Alexa488-conjugated anti-TRA-1-60 antibody (7.5 μg/mL final concentration; STEMCELL Technologies). The cells were stained in 100 μL of diluted antibody per 106 cells overnight at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... H9 ES cells (at 80-90% confluency) were enzymatic detached using dispase (1 mg/ml, Stemcell Technologies, 07923). Detached cells were transferred into medium at 3:1 ratio of mTeSR1 to Neural Induction Medium (NIM ...
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bioRxiv - Developmental Biology 2020Quote: ... Cells were cultured in NeuroCult™ Proliferation Medium containing 20 ng/ml epidermal growth factor (EGF) (Stemcell Technologies). For differentiation assays ...
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bioRxiv - Developmental Biology 2019Quote: ... all medium was removed and 2mL of E6 + 100 ng/mL of bFGF (Stemcell Technologies Inc. Vancouver, Canada) were added ...
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bioRxiv - Bioengineering 2019Quote: ... using density gradient centrifugation on iso-osmotic medium (density: 1.077 g mL−1, Lymphoprep, Stemcell Technologies, Köln, Germany). In short ...
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bioRxiv - Genomics 2021Quote: ... tissue was viably frozen by placing 4 biopsies in 500 ml of CryoStor CS10 freezing media (STEMCELL Technologies). Tubes were inverted and then placed on ice for up to 30 minutes ...
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bioRxiv - Genetics 2021Quote: ... 100ng/ml human interleukin 6 (hIL-6) (all obtained from EuroBiosciences) and 35nM UM171 molecule (Stem Cell Technologies). Cells were cultured under normoxic conditions ...
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bioRxiv - Cell Biology 2021Quote: ... for 5 min followed by a 3 min treatment with 5 mg/mL dispase (Stem Cell Technologies, 07913) and 1 mg/mL DNase I (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... Floating HPCs were then collected and plated at 50,000 cells/mL in STEMdiff Microglia Differentiation media (StemCell Technologies) for 24 days ...
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bioRxiv - Molecular Biology 2022Quote: Two hundred and fifty patient CD34+ HSPCs were seeded in 0.5 mL methocult H4434 (StemCell Technologies, Cat 04434) supplemented with 1% penicillin/streptomycin (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2019Quote: ... cultures were initiated at a density of 500,000 cells/mL with serum-free StemSpan SFEM (Stem Cell Technologies) supplemented with Hydrocortisone (1µM) ...
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bioRxiv - Microbiology 2019Quote: ... Pure populations of sorted HPCs were plated either at 500 cells/mL in Methocult H4434 (Stem Cell Technologies) in 6 well plates in triplicate for myeloid colony assays ...
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bioRxiv - Cancer Biology 2021Quote: ... T-cell cultures were fed with fresh 10% RPMI supplemented with human IL2 (20u/ml Stem Cell Technologies) every 2-3 days to maintain 1×106 cells/mL/mm2 ...
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bioRxiv - Neuroscience 2022Quote: ... Floating HPCs were then collected and plated at 50,000 cells/mL in STEMdiff Microglia Differentiation media (StemCell Technologies) for 24 days ...
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bioRxiv - Developmental Biology 2022Quote: Cardioids (8 cardioids per condition) were dissociated using a 1.5 mL CM dissociation medium (Stem Cell Technologies, #05025) for 7 - 10 min at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... 5 min) and plated at a density of 90,000 cells/mL in STEMdiff Neural Induction Medium (StemCell Technologies) with SMADi supplement (StemCell Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were subjected to red cell lysis in 5 ml ammonium chloride lysis buffer solution (Stem Cell Technologies) on ice for 10 minutes ...
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bioRxiv - Developmental Biology 2023Quote: ... Aggrewells were subjected to a half change with hIVC1 media with 1.25 µg/mL hIGF1 (78022.1 STEMCELL Technologies) and 1µg/mL doxycycline ...
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bioRxiv - Bioengineering 2023Quote: ... T cells were cryopreserved at 2×107 cells per mL in CryoStor CS10 cryopreservation media (Stem Cell Technologies) until use ...
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bioRxiv - Immunology 2023Quote: ... was used at 2 µg/mL.11,14 Immunocult-ACF Human B Cell Expansion Supplement (BAC, Stem Cell Technologies) was used at 1:50 dilution per manufacturer’s instructions.
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Efficient Methods for Target Gene Manipulation in Haematopoietic Stem Cell Derived Human NeutrophilsbioRxiv - Cell Biology 2023Quote: ... Cells were plated at a minimum density of 5 × 105 cells/mL in SFEM II medium (StemCell Technologies) with StemSpanTM CD34+ Expansion Supplement (StemCell Technologies) ...
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bioRxiv - Developmental Biology 2023Quote: Enriched CD34+ cells were resuspended to a final volume of 1.5 mL with semisolid Methocult (StemCell Technologies, #04404) and then plated in 6-cm dishes and incubated at 37°C in 5% CO2 ...
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bioRxiv - Molecular Biology 2023Quote: ... CD34+ HSPCs were cultured at 1×105 cells/mL in StemSpan Serum-Free Expansion Medium II (STEMCELL Technologies) or Good Manufacturing Practice Stem Cell Growth Medium (SCGM ...
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bioRxiv - Immunology 2023Quote: ... Electroporated cells were transferred to cRPMI medium without antibiotics containing 10 ng/mL hrM-CSF (Stem Cell Technologies) and rested at 37°C for 1 h before stimulation ...
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bioRxiv - Bioengineering 2023Quote: ... The resulting sample was resuspended to a concentration of 50 million cells/mL in EasySep Buffer (STEMCELL Technologies). 50 μL/mL isolation cocktail and 50 μL/mL cocktail enhancer were added to the sample ...
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bioRxiv - Cancer Biology 2022Quote: ... The organoids were passage through shear stress with cold 1 U/ml Dispase/DMEM/F12 solution (StemCell Technologies) followed by Trypsin-EDTA (Invitrogen) ...
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bioRxiv - Cancer Biology 2023Quote: ... at 2.5 x 106 cells/mL and ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator (STEMCELL Technologies) at 25μL/mL ...
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bioRxiv - Cell Biology 2023Quote: ... The minced tissue was then placed in an enzyme cocktail consisting of 1U/ml dispase (Stemcell Technologies, 07913), 2,000U/ml collagenase I (Worthington ...
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bioRxiv - Developmental Biology 2023Quote: ... MEF feeders were inactivated at passage 4 with chemical treatment of 1 μg/ml mitomycin-c (STEMCELL Technologies) overnight ...
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bioRxiv - Cell Biology 2023Quote: ... the hESCs were treated with liver differentiation medium (LDM) along with 50 ng/mL Activin A (STEMCELL Technologies), 50 ng/mL WNT3A (PeproTech ...
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bioRxiv - Cancer Biology 2023Quote: Viable BM cells (2×104) were seeded in 1.1 mL Mouse Methylcellulose Complete Media (M3434; Stem Cell Technologies) in uncoated 6 well plates (3471 ...
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bioRxiv - Bioengineering 2024Quote: ... 500 and 1500 CD34+ cells were added to 1 mL of MethoCult™ H4034 Optimum medium (StemCell Technologies) and placed into one well of a 6-well plate ...
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bioRxiv - Immunology 2021Quote: ... ICM T cells were stimulated with ImmunoCult™ Human CD3/CD28 T Cell Activators (25 μl/ml, STEMCELL Technologies). TAC T cells were stimulated with T Cell CD3/CD28 TransAct™ ...
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bioRxiv - Neuroscience 2020Quote: ... Primary mouse microglia and M-MØP cells were exposed at set time points to anti-FcγRII/III (2.4G2) antibody at 5 µg/ml (Stemcell technologies), LPS (50 ng/ml ...
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bioRxiv - Biophysics 2021Quote: ... 05702) and media supplemented with 20ng/ml of human recombinant epithelial growth factor (EGF - STEMCELL Technologies, Cat. No. 78006). All cells were grown in a humidified incubator atmosphere at 37°C and 5% CO2 ...
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bioRxiv - Neuroscience 2022Quote: ... LMMP tissue was digested for 60 minutes at 37° C in dispase (250 μg/ml; STEMCELL Technologies, Vancouver, BC) and g/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were seeded at 5 × 105 cells/ml and grown in Neurocult mouse neurobasal medium (Stem Cell Technologies, 5700) supplemented with 10 ng/ml hEGF (Lonza ...
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bioRxiv - Cell Biology 2022Quote: ... M1 polarized cells were obtained by stimulating MΦ cells with 20 ng/mL of IFNγ (Stemcell Technologies, Cat# 78020) and 100 ng/mL of LPS for 24 h ...
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bioRxiv - Immunology 2021Quote: ... duplicate samples containing 500 sorted cells were plated in 1.5 ml MethoCultTM GF M3434 (STEMCELL Technologies, Catalogue no.- 03434) which already contains SCF ...
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bioRxiv - Bioengineering 2020Quote: ... T cells were frozen at 10–20 × 106 cells per 1 mL of Cryostor CS10 (STEMCELL Technologies, Cambridge, MA) and thawed into culture for knockout experiments.