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Citations for Stemcell Technologies :
651 - 700 of 929 citations for 2 Trimethylsilyl Furo 3 2 B Pyridine 6 Carbaldehyde since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... ESCs were plated on laminin-coated 6 well plates at a density of 65000 cells per well in N2B27 media (as above but with N2 Supplement-B from StemCell Technologies) supplemented with bFGF (10ng/uL bFGF (R&D) ...
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bioRxiv - Immunology 2020Quote: Mouse B cells were isolated from pooled single cell suspensions of spleen and peripheral lymoh nodes using CD19 Microbeads (Miltenyi) or Mouse B cell Isolation Kit (Stemcell Technologies). B cells were stimulated with LPS (10 μg/mL ...
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bioRxiv - Immunology 2022Quote: ... Then all cell clumps formed in Stemdiff B were transferred to a 24-well transwell (0.4uM pore, Stem cell technologies, #38024) for further differentiation ...
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bioRxiv - Immunology 2024Quote: ... + 1:100 Glutamax + 10 mM HEPES + 1 mM N-AcetylCystein + 1:50 B-27 Supplement + 50 ng/ml human EGF + 100 ng/ml human Noggin +100 ng/ml human IGF1 (Stemcell Technologies) + 50 ng/ml human FGF2 (Stemcell Technologies ...
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bioRxiv - Immunology 2023Quote: ... B cell purification was performed according to manufacturer instructions with the Stem Cell EasySep Human Pan-B Cell Enrichment kit (Stemcell Technologies). After enrichment ...
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bioRxiv - Immunology 2023Quote: Small resting B cells were isolated from spleen with a discontinuous Percoll gradient and subsequent purification using EasySep Mouse B cell Isolation Kit (StemCell Technologies). B cells were stimulated with 3mM CpG (Sigma) ...
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bioRxiv - Immunology 2022Quote: ... and B and T cells were isolated using EasySep Negative selection Mouse B and T cell Enrichment Kits according to the manufacturers protocol (StemCell Technologies). Cells were then suspended in transfer buffer (10 mM HEPES ...
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bioRxiv - Genetics 2024Quote: Splenic B cells from BACtg and wild type littermate control mice were isolated using the EasySep Mouse B Cell Isolation Kit (StemCell Technologies) and rested for 30 minutes and then either left unstimulated or stimulated with the addition of 10 μg/ml goat anti-IgM F(ab’)2 crosslinking antibody (Jackson ImmunoResearch ...
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bioRxiv - Cancer Biology 2021Quote: ... in the same 3:4 mixture of media (StemCell Technologies #05620) and Matrigel (BD Bioscience CB-40324) ...
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bioRxiv - Cell Biology 2021Quote: ... and IL-3 (1ng/mL first day only; Stemcell Technologies; Canada). Cell density was maintained between 0.7-2×106 cells/mL (CASY Model TCC ...
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bioRxiv - Cancer Biology 2022Quote: ... recombinant Fms-like tyrosine kinase-3 (Flt3, StemCell Technologies Cat#78009), Erythropoeitin (EPO ...
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bioRxiv - Immunology 2020Quote: ... Following enumeration using 3% acetic acid with methylene blue (StemCell Technologies), splenocytes were resuspended in phosphate-buffered saline and forty to sixty million cells were injected either intraperitoneally or intravenously (via retro-orbital injection under isoflurane anesthesia ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were passaged every 3–5 days with ReLeSR (STEMCELL Technologies). All cells used had a normal diploid karyotype.
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bioRxiv - Neuroscience 2022Quote: ... 3×106 single-cell suspension (Gentle Cell Dissociation Reagent, StemCell Technologies) of iPSCs were plated ...
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bioRxiv - Developmental Biology 2024Quote: ... in motor neuron differentiation medium with 3 uM CHIR99021 (Stemcell Technologies) and cultured for 2 days to form neurospheres ...
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bioRxiv - Genomics 2024Quote: ... 3 x 106 cells were dissociated with Accutase (Stemcell Tech, 7920) and electroporated with 6 μg of gRNA-Cas9 expression vector and 3 μg of SNRPN targeting vector using the P3 Primary Cell 4D-Nucleofector Kit (Lonza ...
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bioRxiv - Neuroscience 2023Quote: ... Split NPCs 1:3 every week with accutase (STEMCELL Technologies; 7920). NPCs were further differentiated into human astrocytes by seeding dissociated single cells at 15,000 cells/cm2 density on Matrigel-coated plates in Astrocyte medium (ScienCell ...
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bioRxiv - Neuroscience 2022Quote: ... and passaged every 3-4 days using ReLeSR (Stem Cell Technologies) until neural induction ...
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bioRxiv - Neuroscience 2022Quote: ... and passaged every 3-4 days using ReLeSR (Stem Cell Technologies). Human iPSCs were edited for homozygous knockout of TAOK2 or heterozygous knock-in of the A135P mutation as described in Deneault et al.(Deneault et al. ...
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bioRxiv - Cell Biology 2022Quote: ... and 3 µM of CHIR99021 (GSK3B inhibitor, STEMCELL Technologies, cat # 72054). From day 1 to 3 ENR was supplemented with 20% Wnt3a-CM and 10 μM Y-27632.
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bioRxiv - Immunology 2021Quote: ... Donor 2395 iPSC were maintained on Matrigel-coated 6 well plates in mTeSR+ medium (StemCell Technologies) and differentiated to sBC as previously described with some modifications (29) ...
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bioRxiv - Cell Biology 2021Quote: ... Cell colonies were separately dissociated and transferred into 6-well plates using ReLeSR (Stem Cell Technologies). Differentiated cells were removed by using a pipette tip to minimize spontaneous differentiation ...
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bioRxiv - Cell Biology 2020Quote: ... Interleukin-6 (10 ng/mL) and Heparin (5 U/mL) (all supplied by Stem Cell Technologies).
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bioRxiv - Neuroscience 2023Quote: ... and plated into a single well of a 6-well Aggrewell plate (Stemcell Technologies Aggrewell 800) that was coated with 2 ml of anti-adherence rinsing solution (Stem cell technologies ...
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bioRxiv - Bioengineering 2022Quote: ... coated 6 well plates were used to culture WA09 cells with mTESR plus medium (Stemcell Technologies) in an incubator at 37 degrees Celsius and 5% CO2 ...
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bioRxiv - Genomics 2022Quote: ... rehydrated tissue was placed into a well of a 6-well plate (Stem Cell Technologies #38015) with 1 mL of ice-cold CST lysis buffer ...
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bioRxiv - Immunology 2022Quote: ... responder CD4+ T cells were isolated from OTII BL/6 mice by negative selection (STEMCELL Technologies). Splenocytes from wild-type or HLA-A2+ BL/6 mice were depleted of Thy1.2+ cells (STEMCELL Technologies) ...
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bioRxiv - Cancer Biology 2021Quote: ... Isolation of B-cells from healthy donor and MM patients were performed using EasySepTM Human B-Cell Isolation Kit according to manufacturer’s protocol (Cat. No. 17954, Stemcell Technologies, Vancouver, Canada).
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bioRxiv - Immunology 2020Quote: ... Naïve B cells were purified from the splenocyte suspension by negative isolation using an EasySep Mouse B Cell Isolation Kit from Stem Cell Technologies, according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... Both hiPSCs and ESCs showed pluripotency markers and normal karyotype throughout the study (Figure S1A-B) and were grown on matrigel-coated plates in mTeSR medium (Stem Cell Technology) containing the Rock inhibitor ...
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bioRxiv - Immunology 2023Quote: ... Primary human B cells were isolated from fresh leuko-reduction collar blood by using 750 µL of RosetteSep™ Human B Cell Enrichment Cocktail (Stemcell Technologies) for 10 mL of collar blood ...
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bioRxiv - Neuroscience 2022Quote: ... iPSCs were first differentiated into hematopoietic progenitor cells using a combined strategy of incorporating primitive hematopoiesis via a WNT switch(77)and sorting and replating CD235a+ cells in Medium B from a commercially available kit (StemCell Technologies #05310). Briefly ...
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bioRxiv - Bioengineering 2023Quote: We isolated B cells from healthy human donors’ PBMCs (Fred Hutchinson Cancer Research Center) using the EasySep Human B cell enrichment kit (Stem Cell Technologies). We obtained >95% purity for B cells defined by CD3 negativity and CD19 positivity ...
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bioRxiv - Immunology 2024Quote: ... splenic P14 TM were enriched at indicated time points after LCMV Arm by removal of APC- or PE-labeled CD4+ T and CD19+ B cells with anti-APC/PE magnetic microbeads (Miltenyi Biotec & STEMCELL Technologies) and 5×103 P14 TM were transferred i.v ...
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bioRxiv - Bioengineering 2022Quote: We isolated B cells from healthy donors’ peripheral blood mononuclear cells (Fred Hutchinson Cancer Research Center) using the EasySep Human B cell isolation kit (Stem Cell Technologies). Isolated B cells were cultured in Iscove’s modified Dulbecco’s medium (Gibco ...
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bioRxiv - Immunology 2024Quote: Culture supernatants were prepared by growing 5×107 B cells purified from peripheral blood of either healthy donors or CLL patients by negative selection using RosetteSep™ Human B-cell enrichment Cocktail (#15064; STEMCELL Technologies), in 15 ml RPMI-1640 medium (#8758 ...
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bioRxiv - Immunology 2024Quote: ... 10 x 106 PBMCs were enriched for B cells using CD3 T cell depletion via the EasySep™ Human CD3 Positive Selection Kit II (StemCell Technologies) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2024Quote: ... B-cells were then purified from the other harvested PBMCs by negative selection using a Human B-cell Isolation Kit (Stem Cell Technologies) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... B cells were then enriched by negative selection using EasySep™ Human B Cell Enrichment Kit II Without CD43 Depletion (17963, Stemcell Technologies) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: Mammary glands were dissected and epithelial cells were isolated via differential centrifugation as previously described.[61] Primary mammary epithelial cells (MECs) were cultured in Epicult-B media with proliferation supplement (Stemcell Technologies, #05610) with 10ng/ml EGF ...
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bioRxiv - Immunology 2024Quote: B cells from tonsils of 5 individuals were enriched using negative selection magnetic beads (EasySep Human B Cell Isolation kit, Stemcell technologies, #17954). They were then pooled to avoid batch effects ...
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bioRxiv - Genomics 2020Quote: ... The cells were passaged every 3–4 days using Accutase (Stemcell Technologies). The iPS cells conversion to EPS as previous reported47.
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bioRxiv - Cell Biology 2020Quote: ... 3 × 106 iPSCs were seeded into an Aggrewell 800 well (STEMCELL Technologies) to form EBs ...
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bioRxiv - Cell Biology 2024Quote: ... Passaging was performed every 3-4 days using ReLeSR (Stemcell Technologies, #05872) according to manufacturer’s instructions ...
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Autism genes converge on microtubule biology and RNA-binding proteins during excitatory neurogenesisbioRxiv - Systems Biology 2024Quote: ... cells were passaged at 1:3 using Accutase (STEMCELL Technologies, Cat#07920) and cultured in NPC medium on Matrigel coated plates onwards.
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Autophagy suppression in DNA damaged cells occurs through a newly identified p53-proteasome-LC3 axisbioRxiv - Cell Biology 2024Quote: ... containing CHIR-99021 (3 μM final concentration; #72052, Stem Cell Technologies, Canada) and Valproic Acid (VPA ...
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bioRxiv - Cell Biology 2024Quote: ... Passaging was performed every 3-4 days using ReLeSR (Stemcell Technologies, #05872) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... and passaged every 3-4 days using Accutase (Stem Cell Technologies, # 07920) The detailed procedure can be found in previous studies28.
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bioRxiv - Bioengineering 2024Quote: ... and anti-mouse TACI-APC (3 μg/ml; STEMCELL Technologies cat# 60116AZ); ii ...
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bioRxiv - Cancer Biology 2024Quote: ... and Fms-like tyrosine kinase-3 Ligand (Flt3, StemCell Technologies, Cat#78009) at 25 ng/mL ...