Labshake search
Citations for Stemcell Technologies :
651 - 700 of 1897 citations for 2'3' cyclic GAMP cGAMP ELISA Kit 384 well Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... monocytes were enriched from cryopreserved PBMCs using EasySep™ Human Monocyte Enrichment Kit without CD16 Depletion Kit (STEMCELL technologies). Enriched monocytes were stimulated with 100ng/ml LPS for 4h at 37 °C before staining ...
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bioRxiv - Cell Biology 2021Quote: ... 5 × 105 cells were pelleted at 350x g for 2 min and transferred onto a 6-well transwell membrane (Stem Cell Technologies, #3450). Pellets were incubated with CHIR99021 (5 µM ...
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bioRxiv - Synthetic Biology 2023Quote: ... cells from each well were dissociated and resuspended in cloning media consisting of mTeSR-Plus supplemented with 1X CloneR2 (StemCell Technologies; 100-0691) and mixed gently with a P1000 pipette tip in order to generate a single-cell suspension ...
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bioRxiv - Cell Biology 2023Quote: ... cells were seeded at a density of 1.1×105 cells/well onto a 12 mm Transwell (ref 38023, STEMCELL TECHNOLOGIES, Saint Égrève, France) and grown in KSFM until cell confluency ...
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bioRxiv - Cell Biology 2020Quote: ... at 37°C for 5 minutes and replated in a ratio of 1:6 or 1:9 on new Matrigel-coated plates in E8 supplemented with 5 μM ROCK inhibitor Y-27632 (Stemcell Technologies, Canada) for 24h ...
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bioRxiv - Developmental Biology 2020Quote: ... Two conventional and established conditions were used as indicated throughout the figures and manuscript: TeSR conditions on Matrigel coated plates (Stem Cell Technologies) or FGF/KSR conditions on MEF substrate ...
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bioRxiv - Molecular Biology 2022Quote: ... were a gift from the Nik-Zainal lab (University of Cambridge, UK) and cultured on non-tissue culture treated plates (Stem Cell Technologies) pre-coated with 10 μg/mL Vitronectin XF (Stem Cell Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... the corrected and patient cell lines were maintained in plates coated with Geltrex (1:50 DMEM/F12) overnight using mTeSR (Stem Cell technologies). The media was refreshed daily for iPSCs ...
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bioRxiv - Developmental Biology 2020Quote: ... Both hiPSCs and ESCs showed pluripotency markers and normal karyotype throughout the study (Figure S1A-B) and were grown on matrigel-coated plates in mTeSR medium (Stem Cell Technology) containing the Rock inhibitor ...
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bioRxiv - Developmental Biology 2022Quote: KOLF2 iPS cells were grown under feeder-free conditions on Matrigel-coated plates in TeSR™-E8™ (Stem Cell Technologies, #05990).
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bioRxiv - Synthetic Biology 2022Quote: Human iPSC line PGPC-17/11 (Personal Genome Project Canada) was cultured on Matrigel-coated plates in mTeSR Plus medium (STEMCELL Technologies 05825) and passaged with ReLeSR (STEMCELL Technologies 05873 ...
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bioRxiv - Neuroscience 2022Quote: ... incubated for 15 min at 37°C and adherent aNSCs/NPCs were lifted from the plate using 1 mL of Accutase (STEMCELL Technologies, 07920) incubated for 5 min at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... and 2.8×105 cells/cm2 were plated on Geltrex-coated plates in mTesR+ supplemented with ROCK inhibitor Y-27632 (10uM, StemCell Technologies, 72302). Neuronal induction was started 24h later (day 0 ...
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A scalable, GMP-compatible, autologous organotypic cell therapy for Dystrophic Epidermolysis BullosabioRxiv - Bioengineering 2023Quote: ... after expansion the cells were prepared for embryoid body formation using the AggreWell™ EB 400 microwell plate following the manufacturers recommendations (StemCell Technologies). Cells were dissociated in Accutase (Innovative Cell Technologies ...
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bioRxiv - Developmental Biology 2023Quote: PP cells were dissociated using TrypLE Express at 37°C for 2-3 mins and seeded in microwells of AggreWell 800 plates (STEMCELL Technologies, 34825), using the recommended procedure by the supplier ...
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bioRxiv - Molecular Biology 2024Quote: ... Definitive hindgut endoderm was generated as reported.8 Induced cells were harvested and seeded on AggreWellTM400 Microwell Plate (StemCell Technologies Cambridge, MA) at 3.6 million cells in each well for 24 hours ...
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bioRxiv - Cell Biology 2024Quote: ... 200-500 µm EBs were picked under a dissecting microscope and transferred onto adherent tissue culture plates (2.5 EBs/cm2) for cultivation in Hematopoietic Differentiation (HD) medium (APEL2 (Stem Cell Tech 05270), with 0.5 % Protein free hybridoma medium (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: MDA-MB-468 and mitomycin-C treated NIH-3T3 cells were plated at a ratio of 10:1 in AggreWellTM400 plates at approximately 50 cells/microwell according to the manufacturer’s instructions (#34415, Stemcell Technologies, Vancouver, BC) and grown for 5 days in tumor media containing 1μg/mL doxycycline ...
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bioRxiv - Immunology 2023Quote: ... 1 mM EDTA) and isolated using a negative isolation kit (EasySep™ Mouse B Cell Isolation Kit, #19854, StemCell Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... and plated at 3 × 106 cells/well to allow the embryoid body (EB) formation in Neural Induction Media (NIM) + 10 μM Y-27632 (Stem Cell Technologies, Cat.No. 72308). They were allowed to attach for at least overnight ...
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bioRxiv - Bioengineering 2023Quote: ... containing 300 cells and with 10ng/ml IL-3 (+IL-3 treatment) or without IL-3 (-IL-3 treatment) were plated in a well of a SmartDish (StemCell Technologies, Seattle, WA, USA) in duplicates ...
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bioRxiv - Microbiology 2022Quote: RosetteSep and EasySep negative isolation kits (Stemcell Technologies) were used sequentially to isolate CD19+ B-cells by negative selection ...
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bioRxiv - Microbiology 2020Quote: ... PE positive selection kit (STEMCELL Technologies, https://www.stemcell.com) and Monocyte negative selection kit (STEMCELL Technologies ...
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bioRxiv - Microbiology 2020Quote: ... CD8 positive selection kit (STEMCELL Technologies, https://www.stemcell.com), PE positive selection kit (STEMCELL Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... CellSep Human CD19+ selection kit (Stem Cell Technologies) was used to enrich for CD19+ cells ...
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Salinomycin inhibits epigenetic modulator EZH2 to enhance Death Receptors in Colon Cancer Stem CellsbioRxiv - Cancer Biology 2020Quote: ... Aldefluor kit was bought from Stem cell technologies. Magnetic ChIP kit (Cell Signaling Technology) ...
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bioRxiv - Bioengineering 2020Quote: Negative selection using RosetteSep kits (Stem Cell Technologies) was adopted to isolate primary human T cells from healthy volunteer donors following leukapheresis ...
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bioRxiv - Cancer Biology 2022Quote: The ALDEFLUOR kit (StemCell Technologies, Durham, NC, USA) was used to analyze the population with high ALDH enzymatic activity according to the manufacturer’s intructions ...
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bioRxiv - Genetics 2020Quote: ... STEMdiff Cerebral Organoid kit (Stem Cell Technologies, 08570) was used for organoid culture with timing according to manufacturer’s suggestion and Matrigel embedding as previously described57 ...
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bioRxiv - Immunology 2022Quote: ... and CD8+ T-cell Isolation Kit (StemCell Technologies #19851 ...
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bioRxiv - Neuroscience 2022Quote: ... using the STEMdiff cerebral organoid kit (STEMCELL Technologies) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... the STEMdiff Definitive Endoderm Kit (STEMCELL Technologies, 05110), was used for differentiation into endoderm ...
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bioRxiv - Immunology 2023Quote: ... EasySep Human CD34+ Positive Selection Kit (Stemcell Technologies) was used to enrich for CD34+ cells to >90% purity.
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bioRxiv - Immunology 2023Quote: ... and EasySep Human Monocyte Isolation Kit (StemCell Technologies). In the coculture experiment ...
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Chimeric Antigen Cytotoxic Receptors for In-Vivo Engineering of Tumor-targeting Natural Killer CellsbioRxiv - Immunology 2023Quote: ... ImmunoCult™ NK cell-expansion kit (StemCell Technologies) was used following the manufacturer’s recommendations to activate isolated NK cells in vitro for 6 days ...
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bioRxiv - Immunology 2023Quote: ... using a negative selection isolation kit (STEMCELL Technologies), and purity was confirmed to be >97% ...
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bioRxiv - Neuroscience 2021Quote: ... cells were plated on Matrigel (In Vitro Technologies, cat. # FAL354277) coated plates in StemFlex medium supplemented with 10 μM ROCK inhibitor (StemCell Technologies, cat. #. 72304). Three days after plating ...
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bioRxiv - Pathology 2020Quote: ... human iPS-GFP IMR-90-4 reporter cells were maintained in an undifferentiated state in mTesr1 media on plates coated with Matrigel and were routinely passaged with Versene (StemCell Technologies, Vancouver, CA) in a ratio of 1:6 ...
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bioRxiv - Genetics 2020Quote: ... Transduced cells were expanded to 10 cm plates and 10 vials (2M cells/vial) of each cell line was cryopreserved in CryoStor® (StemCell Technologies, 07930) for further use.
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bioRxiv - Cell Biology 2020Quote: . Primary human CD3+ and CD8+ T cells were isolated from unfractionated PBMCs using the EasySep Human T cell Isolation Kit and Human CD8 T cell isolation kit with RapidSpheres (Stemcell Technologies). Mobilized human primary CD34+ cells from Peripheral Blood (were obtained from Caltag Medsystems ...
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bioRxiv - Bioengineering 2020Quote: ... CD4pos and CD8pos T cells were isolated by negative selection using EasySep Human CD4+ T Cell Isolation Kit and EasySep Human CD8+ T Cell Isolation Kit (STEMCELL Technologies), respectively ...
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bioRxiv - Immunology 2021Quote: ... followed by enrichment of either effector/memory T cells (Human Memory CD4 T cell Enrichment kit; EasySep) or Treg cells (Human CD4+CD127loCD49d− Treg Enrichment Kit; EasySep) (all from StemCell Technologies).
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bioRxiv - Immunology 2022Quote: CD4+ T cells and CD25hi CD4+ T cells were negatively isolated from PBMCs separately by using Easysep human CD4+ T cell isolation kits and EasySep Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit (STEMCELL Technologies), respectively ...
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bioRxiv - Immunology 2023Quote: ... the dead cells were first removed with a Dead Cell Removal Kit and then the NK cells were depleted using the EasySep Human CD56 Positive Selection Kit (Stemcell Technologies). The control 221 cells were kept in the same culture conditions but were not exposed to NK cells ...
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bioRxiv - Neuroscience 2023Quote: NPC (iPSC-derived) patterning toward forebrain neuron fate was conducted using a commercially available kit (STEMdiff Forebrain Neuron Differentiation Kit, STEMCELL Technologies). Cells were maintained in forebrain neuron differentiation medium for 6 days before being dissociated into a single-cell solution and plated onto either Poly-L-Lysine- and Laminin-coated glassware/ Geltrex-coated plasticware at varying densities (6 well plate – 5.2x104 cells/cm2 ...
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bioRxiv - Neuroscience 2023Quote: Human iPSC-derived microglia (iMGL) were generated from human iPSC-derived hematopoietic progenitors using the STEMdiff Microglia Differentiation Kit and Microglia Maintenance Kit (STEMCELL Technologies) according to manufacturer instructions ...
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bioRxiv - Biochemistry 2023Quote: ... neutrophils were purified by an in-house negative selection kit based on a modified mouse-neutrophil positive selection kit EasySep (Stemcell Technologies). The antibodies used for the negative selection were ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T cells and CD8+ T cells were enriched using the EasySep Human CD4+ T Cell Isolation Kit and EasySep Human CD8+ T Cell Isolation Kit (STEMCELL Technologies), respectively ...
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bioRxiv - Immunology 2024Quote: ... CD4 T cells were isolated from the spleens of young (2- to 3-month-old) mice spleen using a magnetic microbeads negative selection kit (EasySep Mouse CD4 T Cell Isolation Kit; STEMCELL Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... APC-eFluor780-conjugated cKit and 7AAD and purified by fluorescent activated cell sorting (FACS) at a density of 600 Lin- Sca1+cKit+7AAD- cells per 1.1 ml/well of MethoCult™ M3334 (STEMCELL Technologies, Vancouver, British Columbia, Canada). M3334 methocult was supplemented with cytokines to a final concentration of 5 ng/ml mIL-3 ...