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Citations for Stemcell Technologies :
601 - 650 of 828 citations for tert Butyl trans 17 bromo 4 7 10 13 tetraoxa 15 heptadecenoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... CD34+ umbilical cord and CD34+ hematopoietic stem/progenitor cell (Ethics Committee Salzburg approval 415-E/1776/4-2014) were isolated using density gradient centrifugation (Lymphoprep; Stemcell Technologies). Primary human cells ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were checked daily for differentiation and were passaged every 3-4 days using Gentle dissociation solution (STEMCELL Technologies, cat # 07174). All experiments were performed under the supervision of the Vanderbilt Institutional Human Pluripotent Cell Research Oversight (VIHPCRO ...
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bioRxiv - Immunology 2024Quote: ... were differentiated into TH2 T cell subtype by incubation with specific supplement for TH2 differentiation (mouse IL-2, mouse IL-4 and rat anti mouse IFNγ with 1:100 dilution (STEMCELL Technologies)) and incubated for 3 days on plates coated with aCD3 and aCD28 antibodies ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then washed with basal medium (500g, 4°C) and replated in Matrigel in mouse IntestiCult Organoid Growth Medium (STEMCELL Technologies) supplemented with Y-27632 (10 μM) ...
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bioRxiv - Neuroscience 2023Quote: hiPSC line (IMR90-4, WiCell) derived from a healthy donor and fully characterized was cultured with mTeSRTM Plus Medium (STEMCELL Technologies) on growth factor reduced matrigel-coated 6-well plates (Corning ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were split by dissociating to small clusters and replating at a 1:3-1:4 ratio using ReLeSR (StemCell Technologies) once cell clusters reached ∼70% coverage of the culture surface ...
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bioRxiv - Neuroscience 2023Quote: ... cells were transferred into TeSR-E7 medium and maintained until the emergence of iPSC colonies (about 4 weeks) which were then manually isolated and propagated in mTeSR medium (StemCell Technologies). Absence of plasmid integration and clearance of Sendai virus were confirmed ...
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bioRxiv - Biophysics 2023Quote: ... hiPSCs were seeded onto Matrigel-coated 6-well tissue culture treated plates and maintained for 4 days with mTeSR1 media (85850, Stemcell Technologies). On day 0 ...
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bioRxiv - Neuroscience 2024Quote: ... iPSC cultures were passaged every 4-5 days when the colonies reached 80% confluency using the dissociation reagent ReLeSR (STEMCELL Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: Human induced pluripotent stem cell (hiPSCs) were obtained from WiCell (IMR90-4/WISCi004, WiCell) and cultured in mTeSR+ medium (STEMCELL Technologies) supplemented with 1% (v/v ...
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bioRxiv - Neuroscience 2024Quote: ... then 1 mL medium was replaced in a drop-wise manner every day for the next 4 days with an EB medium: mTeSR1 medium (STEMCELL Technologies) containing 50 ng/mL BMP-4 (GIBCO-PHC9534) ...
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bioRxiv - Immunology 2024Quote: ... CD14-enriched cells were resuspended in ImmunoCult™ XF supplemented with 100 U/mL penicillin-streptomycin and cultured in the presence of 50 ng/mL GM-CSF and 100 ng/mL IL-4 (both from STEMCELL Technologies) for 7-days ...
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bioRxiv - Cancer Biology 2024Quote: ... 5×104 K562 cells were seeded in 200 µL K562 medium and 4×104 iPSC-HSPCs were seeded in 200 µL StemSpan SFEM II (StemCell Technologies) supplemented with 1% P/S ...
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bioRxiv - Biochemistry 2024Quote: ... was induced by replacing M-CSF-containing medium with medium containing 100 ng/mL IL-4 (#78045, STEMCELL Technologies, Cologne, Germany) and further incubation for 48 h ...
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bioRxiv - Cancer Biology 2021Quote: Cells were plated on uncoated low-adhesion 96-well plates in a two-fold dilution series spanning from 2000 down to 4 cells per well in NeuroCult NS-A media (StemCell Technologies, #05751) containing doxycycline at 2 μg/mL ...
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bioRxiv - Molecular Biology 2022Quote: ... The medium was changed daily and the cells were passaged every 4–8 days depending on confluency using Gentle Cell Dissociation Reagent (Stem Cell Technologies). 10 μM of ROCKi (Stem Cell Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... containing 10% fetal bovine serum and subsequently resuspended at a concentration of 5×105 cells/mL in 4-5mL of MethoCult H4230 methylcellulose (STEMCELL Technologies, 04230) containing penicillin/streptomycin ...
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bioRxiv - Cell Biology 2021Quote: ... washed with phosphate buffered saline (PBS) and incubated overnight at 4 °C with a mouse monoclonal anti-CD3 (clone UCHT1, StemCell Technologies, #60011) 10 μg/mL in PBS ...
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bioRxiv - Immunology 2021Quote: ... Cells were fed for 4-5 days until airlifted and continued to be grown at air-liquid interface (ALI) with PneumaCult ALI medium (StemCell Technologies, #05001) until fully differentiated (4 weeks)
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bioRxiv - Cell Biology 2020Quote: ... washed with phosphate buffered saline (PBS) and incubated overnight at 4° C with a mouse monoclonal anti-CD3 antibody (clone UCHT1, StemCell Technologies, #60011) or a mouse monoclonal anti-HA antibody (clone 16B12 ...
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bioRxiv - Neuroscience 2022Quote: aNSCs/NPCs were cultured as described above (see Primary NSC cell culture) until Passage 2-4 and then passaged with Accutase (STEMCELL Technologies, 07920) and seeded at a density of 5,000 cells per well on Matrigel (Corning ...
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bioRxiv - Neuroscience 2022Quote: NSCs were cultured as described above (see “Primary NSC cell culture”) in complete activated media until Passage 2-4 and then passaged with Accutase (STEMCELL Technologies, 07920) and seeded at a density of 5,000 cells per well in Matrigel (Corning ...
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bioRxiv - Bioengineering 2023Quote: IMR90-4 iPSCs (WiCell, Madison, WI) were cultured on Matrigel-coated 6-well plates and supplemented daily with E8 medium (Stem Cell Technologies) as described (81) ...
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bioRxiv - Cancer Biology 2023Quote: ... Organoids were passaged every 3-4 days by dissolving the Matrigel in Gentle Cell Dissociation Reagent (GCDR - STEMCELL Technologies cat#100-0485). Organoids were broken down to smaller sizes by trituration in GCDR before washing with basal media (advanced DMEM/F12 ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were checked daily for differentiation and were passaged every 3-4 days using Gentle cell dissociation solution (STEMCELL Technologies, cat # 07174). All experiments were performed under the supervision of the Vanderbilt Institutional Human Pluripotent Cell Research Oversight (VIHPCRO ...
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Autophagy suppression in DNA damaged cells occurs through a newly identified p53-proteasome-LC3 axisbioRxiv - Cell Biology 2024Quote: ... organoids were removed from Matrigel and passaged with a split ratio of 1:4 using the protocol published by Stem Cell Technologies for use with their mouse IntestiCult system ...
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bioRxiv - Cell Biology 2021Quote: ... and then replated in media supplemented with 10 µM ROCK inhibitor (Y-27632, StemCell Technologies, #72304). For the transduction of the virus ...
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bioRxiv - Cell Biology 2022Quote: ... medium was supplemented with 20% Wnt3a-CM and 10 μM Y-27632 (ROCK inhibitor, STEMCELL Technologies). For the first day ...
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bioRxiv - Cell Biology 2020Quote: ... Interleukin-6 (10 ng/mL) and Heparin (5 U/mL) (all supplied by Stem Cell Technologies).
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bioRxiv - Developmental Biology 2021Quote: ... coated dishes at 37°C under hypoxia conditions (10% CO2/5%O2) in mTeSR1 (StemCell Technologies). These were passaged every 4-6 days with Accutase (#A6964 ...
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bioRxiv - Neuroscience 2022Quote: ... seeded onto Matrigel-coated culture plates supplemented with 10 µM Y-27632 (Cat: #72308, StemCell Technologies) and incubated at 32°C (5% CO2 ...
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bioRxiv - Neuroscience 2022Quote: ... KSR and N2 medium were freshly supplemented with 10 µM SB431542 (STEMCELL Technologies, Vancouver, Canada, 72234), 1 µM LDN193189 (STEMCELL Technologies ...
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bioRxiv - Immunology 2022Quote: ... a companion set of age and sex matched deidentfied PBMCs from 10 healthy donors (Stemcell Tech) were included for comparison along with batch control leukophoresis derived PBMCs ...
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bioRxiv - Genomics 2024Quote: ... and transduced in suspension in a matrigel-coated 10-cm dish in mTesR (Stemcell Tech 85850) supplemented with 10 μM Rock Inhibitor (Y-27632 ...
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bioRxiv - Neuroscience 2023Quote: ... IPSCs were passaged 10 times before being transferred to the mTeSR1 culture system (StemCell Technologies, 85875).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and MEM NEAAs (10 μL/mL) in neurobasal medium] following dissociation with Accutase (Stemcell Technologies, 07922). NC-C medium was refreshed on day 14 prior to the induction of cranial neural crest derived peripheral neurons on day 15.
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bioRxiv - Cell Biology 2023Quote: Human induced pluripotent stem cells (iPSCs) were maintained on vitronectin XFTM (10 μg/mL, StemCell Technologies)-coated plates and in Essential 8 (E8 ...
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bioRxiv - Neuroscience 2023Quote: ... The first 10 days of differentiation are carried out in iHPC medium: IMDM (50%, Stemcell technologies), F12 (50% ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were treated overnight with 10 µM Rock inhibitor Y-27632 (Cat. # 72304, Stem Cell Technologies) to attenuate cell death ...
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bioRxiv - Cancer Biology 2024Quote: ... 10 ng/mL Human Recombinant bFGF) and 2 μg/mL Heparin Solution (Stemcell Technologies Canada Inc.). For a subset of experiments neurospheres were moved to Complete Medium (CM ...
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bioRxiv - Neuroscience 2024Quote: ... Upon thawing iPSCs were plated in media containing 10 µM Y27632 - ROCK inhibitor (STEMCELL Technologies, 72304) for 24 hrs ...
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bioRxiv - Developmental Biology 2024Quote: ... in 2 ml EB Medium A supplemented with 10 µM ROCK inhibitor Y-27632 (STEMCELL Technologies) and incubated at 37°C ...
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bioRxiv - Bioengineering 2021Quote: Testicular organoids were made by mixing germ and somatic cells at a 1:4 ratio and placing them into AggreWell800™ microwells (STEMCELL Technologies, 34811) overnight ...
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bioRxiv - Immunology 2023Quote: ... were cultured for 4 days post-split in IntestiCult™ Organoid Growth Medium and then switched IntestiCult™ Organoid Differentiation Medium (STEMCell Technologies) for 72 hours to promote epithelial stem cell differentiation ...
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bioRxiv - Developmental Biology 2023Quote: ... differentiating cells were confluent again and split 1:3-1:4 into Step 2 differentiation medium: BrainPhys Neuronal Medium (STEMCELL Technologies, cat# 05790), 1X N2 ...
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bioRxiv - Bioengineering 2023Quote: ... IMR90-4 iPSCs (WiCell, WB65317) between passages p35∼45 were maintained on Matrigel-coated dishes in mTeSR Plus medium (STEMCELL Technologies, 100-0276) and dissociated using ReLeSR (STEMCELL Technologies ...
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bioRxiv - Neuroscience 2024Quote: ... ESCs were fed daily and were passaged every 3-4 days at a 1:6 ratio using ReLeSR™ (STEMCELL Technologies, #100-0483) following manufacturer protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... media supplemented with 10% FBS (WISENT, ST-BRUNO, QC, Canada) and collagenase (STEMCELL Technologies, Vancouver, BC, Canada), and further dissociated in 5 mg/ml dispase for 2min ...
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bioRxiv - Cell Biology 2022Quote: ... based enzymatic dissociation method was employed for passaging and 10 µM Y-27632 (Stem Cell Technologies, 72307) was supplied on the first day of each passage.
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bioRxiv - Cell Biology 2022Quote: ... based enzymatic dissociation method was employed for passaging and 10 µM Y-27632 (Stem Cell Technologies, 72307) was supplied on the first day of each passage.