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Citations for Stemcell Technologies :
601 - 650 of 1626 citations for Mouse Eukaryotic translation initiation factor 4E binding protein 3 EIF4EBP3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... we changed the medium to a 1:1 mixture of KSR and N2B medium (DMEM F12, Thermo Fisher 11320033, with 1% GlutaMAX, 3% dextrose, N2-Supplement B, StemCell Technologies 07156 ...
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bioRxiv - Immunology 2024Quote: Vascular immune organoids (VIOs) were washed with PBS 3 times and were dissociated by 30 mins incubation of accumax (StemCell Technologies, 07921). Cells were washed once and resuspended in 100μl blocking buffer (2%FBS and 1% FcX (BioLegend ...
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bioRxiv - Cell Biology 2019Quote: Whole-brain cerebral organoids were generated with a commercial kit (STEMdiff Cerebral Organoid Kit, STEMCELL Technologies, Catalogue Number 08570), which has been adopted and optimized based on previously published protocol50 ...
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bioRxiv - Immunology 2024Quote: ... monocytes were enriched from cryopreserved PBMCs using EasySep™ Human Monocyte Enrichment Kit without CD16 Depletion Kit (STEMCELL technologies). Enriched monocytes were stimulated with 100ng/ml LPS for 4h at 37 °C before staining ...
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bioRxiv - Cancer Biology 2022Quote: ... Our murine neurosphere media consisted of 45 ml Neurocult Mouse Basal media (Stem Cell Technologies, Catalog# 05700), 5 ml of mouse Neurocult Proliferation Supplement (Stem Cell Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... mouse embryonic fibroblasts (MEF) and HeLa cells were cultivated in Dulbecco’s modified Eagle’s medium (DMEM) (Stemcell technologies) supplemented with 10% fetal bovine serum (Life Technologies ...
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bioRxiv - Immunology 2020Quote: Intestinal crypts were dissociated from mouse small intestine using Gentle Cell Dissociation Reagent (Stemcell Technologies, Cambridge, UK). The crypts were then re-suspended in Intesticult medium (Stemcell Technologies ...
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bioRxiv - Physiology 2022Quote: Mouse jejunal section were used to isolate crypts according to the manufacturer’s instructions (STEMCELL Technologies, document 28223). Complete IntestiCult medium was exchanged every 2-3 days and organoid numbers and de novo crypts were scored on days 5 and 7 ...
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bioRxiv - Cancer Biology 2023Quote: ... The mouse MLL-AF9-transduced cells were cultured in MethoCult™ M3234 (STEMCELL Technologies, Bancouber, BC, Canada). The human MLL-AF9-transduced cells were cultured in StemSpan SFEM II medium (#ST-09655 ...
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bioRxiv - Cell Biology 2022Quote: ... mouse intestinal organoids were cultured in 24-well plates using IntestiCult organoid growth medium (06005, STEMCELL Technologies). In short ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were cultured for 7 d at 5% O2 in EpiCult- B mouse medium (STEMCELL Technologies, 05610) supplemented with 5% FBS ...
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bioRxiv - Immunology 2024Quote: ... Mouse myeloid cells were differentiated in IMDM (Cytiva, SH30228.FS) supplemented with 20% BIT9500 (StemCell Tech, 09500), 20 ng/mL M-CSF (Peprotech ...
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bioRxiv - Developmental Biology 2023Quote: ... and kept on Rho-associated protein kinase (ROCK) inhibitor Y-27632 (final concentration 5 μM, StemCell Technologies, #72302) for one day ...
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bioRxiv - Cell Biology 2019Quote: ... Differentiation was initiated by changing to RPMI1640 medium containing B-27 (minus insulin) and the glycogen synthase kinase-3 (GSK3) inhibitor CHIR99021 (STEMCELL Technologies, cat. 72052) for 24 h ...
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bioRxiv - Cell Biology 2022Quote: ... the tubuloids still inside the BME were washed 3 times with cold HBSS followed by a 20 min incubation with Dispase solution (StemCell Technologies, Cambridge, UK) at 37°C and 5% v/v CO2 to remove the BME around the tubuloids ...
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bioRxiv - Cancer Biology 2022Quote: ... endothelial and haematopoietic lineages in a 3-stage process as described in Figure 1A and the main manuscript text using APEL2 (StemCell Technologies Cat#05275)(61 ...
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bioRxiv - Microbiology 2021Quote: ... A nasal brushing of the turbinate was acquired using 3-mm bronchial cytology brush and placing the biopsy into warm PneumaCult-Ex Plus Medium (STEMCELL Technologies, Cambridge, UK). The cells were dissociated from the brush by gentle agitation before seeding into a single well of a collagen (PureCol from Sigma Aldrich ...
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bioRxiv - Biophysics 2020Quote: ... were grown and maintained at 37°C in hypoxic conditions (3% O2, 5% CO2) in NeuroCult™ NS-A Proliferation medium (STEMCELL Technologies, 05751) supplemented with 1% Heparin Solution (STEMCELL Technologies ...
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bioRxiv - Developmental Biology 2023Quote: ... differentiating cells were confluent again and split 1:3-1:4 into Step 2 differentiation medium: BrainPhys Neuronal Medium (STEMCELL Technologies, cat# 05790), 1X N2 ...
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bioRxiv - Neuroscience 2023Quote: ... The microglia cell differentiation through 3 steps: 1) Embryoid Body (EB) formation: using Spin-EBs formation method with AggreWellsTM800 (Stemcell Technologies, Catalog # 34825). briefly ...
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bioRxiv - Cell Biology 2023Quote: ... 3.3 x 105 cells and 2.6 x 105 cells per ml were seeded in 3 ml E8 medium supplemented with 10 µM Rho-associated coiled-coil kinase (ROCK) inhibitor Y-27632 (RI, STEMCELL Technologies, Vancouver, Canada), respectively ...
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bioRxiv - Neuroscience 2023Quote: ... On day 3 cells were changed into a 50/50 ratio of fibroblast media and TeSR™ E-7™ Reprogramming Media (STEMCELL Technologies), and on day 5 were changed into 100% E-7™ Reprogramming Media ...
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bioRxiv - Neuroscience 2023Quote: ... 3 million iPSCs per cell line were plated into 1 well of an AggreWell™800 24-well plate (Stem Cell Technologies, 34811) with 2 mL STEMdiff™ Neural Induction Medium + SMADi + 10 µM Y-27632 ...
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bioRxiv - Microbiology 2022Quote: RosetteSep and EasySep negative isolation kits (Stemcell Technologies) were used sequentially to isolate CD19+ B-cells by negative selection ...
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bioRxiv - Microbiology 2020Quote: ... PE positive selection kit (STEMCELL Technologies, https://www.stemcell.com) and Monocyte negative selection kit (STEMCELL Technologies ...
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bioRxiv - Microbiology 2020Quote: ... CD8 positive selection kit (STEMCELL Technologies, https://www.stemcell.com), PE positive selection kit (STEMCELL Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... CellSep Human CD19+ selection kit (Stem Cell Technologies) was used to enrich for CD19+ cells ...
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Salinomycin inhibits epigenetic modulator EZH2 to enhance Death Receptors in Colon Cancer Stem CellsbioRxiv - Cancer Biology 2020Quote: ... Aldefluor kit was bought from Stem cell technologies. Magnetic ChIP kit (Cell Signaling Technology) ...
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bioRxiv - Bioengineering 2020Quote: Negative selection using RosetteSep kits (Stem Cell Technologies) was adopted to isolate primary human T cells from healthy volunteer donors following leukapheresis ...
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bioRxiv - Cancer Biology 2022Quote: The ALDEFLUOR kit (StemCell Technologies, Durham, NC, USA) was used to analyze the population with high ALDH enzymatic activity according to the manufacturer’s intructions ...
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bioRxiv - Genetics 2020Quote: ... STEMdiff Cerebral Organoid kit (Stem Cell Technologies, 08570) was used for organoid culture with timing according to manufacturer’s suggestion and Matrigel embedding as previously described57 ...
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bioRxiv - Immunology 2022Quote: ... and CD8+ T-cell Isolation Kit (StemCell Technologies #19851 ...
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bioRxiv - Neuroscience 2022Quote: ... using the STEMdiff cerebral organoid kit (STEMCELL Technologies) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... the STEMdiff Definitive Endoderm Kit (STEMCELL Technologies, 05110), was used for differentiation into endoderm ...
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bioRxiv - Immunology 2023Quote: ... EasySep Human CD34+ Positive Selection Kit (Stemcell Technologies) was used to enrich for CD34+ cells to >90% purity.
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bioRxiv - Immunology 2023Quote: ... and EasySep Human Monocyte Isolation Kit (StemCell Technologies). In the coculture experiment ...
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Chimeric Antigen Cytotoxic Receptors for In-Vivo Engineering of Tumor-targeting Natural Killer CellsbioRxiv - Immunology 2023Quote: ... ImmunoCult™ NK cell-expansion kit (StemCell Technologies) was used following the manufacturer’s recommendations to activate isolated NK cells in vitro for 6 days ...
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bioRxiv - Immunology 2023Quote: ... using a negative selection isolation kit (STEMCELL Technologies), and purity was confirmed to be >97% ...
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bioRxiv - Neuroscience 2020Quote: Feeder-free E14Tg2a (Baygenomics) mouse embryonic stem cells (mES) were passaged on gelatin-coated (0.1% gelatin, StemCell Technologies) cell culture treated plastic dishes using established media and cell culture techniques (Sadegh and Macklis ...
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bioRxiv - Neuroscience 2023Quote: ... cells were cultured into 96-well low-attachment plates containing NeuroCult Basal Media (Mouse and Rat; STEMCELL Technologies) with Proliferation Supplement (STEMCELL Technologies) ...
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bioRxiv - Cancer Biology 2023Quote: Viable BM cells (2×104) were seeded in 1.1 mL Mouse Methylcellulose Complete Media (M3434; Stem Cell Technologies) in uncoated 6 well plates (3471 ...
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bioRxiv - Neuroscience 2019Quote: ... cells were rinsed with phosphate-buffered saline (PBS) (5 ml) and detached by addition of ‘Gentle Dissociation Reagent’ (3 ml) (STEMCELL Technologies; Cat. No. 07174) stored at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... the isolated intestinal tissue was cut into ∼3-to 5-mm pieces and incubated in Gentle Cell Dissociation Reagent (Stem Cell Technologies, Vancouver, Canada). After removal of the Gentle Cell Dissociation Reagent ...
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bioRxiv - Systems Biology 2024Quote: ... mouse NGN2 at the AAVS1 locus and pC13N-dCas9-BFP- KRAB at human CLYBL intragenic safe harbor locus (between exons 2 and 3) were cultured in mTeSR Medium (Stemcell Technologies; Cat. No. 85850) on Corning Tissue Culture Treated Dishes (VWR International LLC ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were cultured in mTeSR plus medium supplemented with 10 µM Rho-associated protein kinase (ROCK) inhibitor (Stemcell Technologies). After overnight incubation ...
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bioRxiv - Biophysics 2023Quote: ... and the media further supplemented with 30 U mL-1 of human recombinant IL-2 protein (STEMCELL Technologies, USA). T cells were incubated in a humidified incubator with 5% CO2 at 37°C and were split to 0.5×106 cells mL-1 when their concentration surpassed 2.5×106 cells per well or when medium became depleted (turning yellow in color) ...
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bioRxiv - Cell Biology 2020Quote: . Primary human CD3+ and CD8+ T cells were isolated from unfractionated PBMCs using the EasySep Human T cell Isolation Kit and Human CD8 T cell isolation kit with RapidSpheres (Stemcell Technologies). Mobilized human primary CD34+ cells from Peripheral Blood (were obtained from Caltag Medsystems ...
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bioRxiv - Bioengineering 2020Quote: ... CD4pos and CD8pos T cells were isolated by negative selection using EasySep Human CD4+ T Cell Isolation Kit and EasySep Human CD8+ T Cell Isolation Kit (STEMCELL Technologies), respectively ...
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bioRxiv - Immunology 2021Quote: ... followed by enrichment of either effector/memory T cells (Human Memory CD4 T cell Enrichment kit; EasySep) or Treg cells (Human CD4+CD127loCD49d− Treg Enrichment Kit; EasySep) (all from StemCell Technologies).
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bioRxiv - Immunology 2022Quote: CD4+ T cells and CD25hi CD4+ T cells were negatively isolated from PBMCs separately by using Easysep human CD4+ T cell isolation kits and EasySep Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit (STEMCELL Technologies), respectively ...