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Citations for Stemcell Technologies :
601 - 650 of 2041 citations for Human Rh Blood Group D Antigen RHD CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated through Lymphoprep density gradient separation (Stemcell Technologies). Cells were used after cryopreservation.
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bioRxiv - Microbiology 2019Quote: ... freshly isolated PBMCs obtained from healthy donor peripheral blood (lymphocyte separation media; StemCell Technologies) were subjected to CD14+ magnetic bead positive selection using the MojoSort Human CD14 Selection Kit (BioLegend) ...
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bioRxiv - Cancer Biology 2019Quote: Red blood cell lysis was first performed using Ammonium Chloride solution from STEMCELL Technologies for 10 minutes on ice ...
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bioRxiv - Bioengineering 2021Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated using Lymphoprep density gradient centrifugation (StemCell Technologies). CD8+ T cells were isolated from PBMCs by positive magnetic selection using EasySep Human CD8 Positive Selection Kit (StemCell Technologies) ...
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bioRxiv - Microbiology 2020Quote: ... The diluted blood was added to a SepMate™-50 centrifugation tube (Stemcell Technologies) under-layered with Histopaque®-1083 (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2019Quote: ... peripheral blood mononuclear cells (PBMCs) were processed from Leukopak (STEMCELL Technologies, Vancouver, BC, Canada). Monocytes were isolated from PBMCs using the EasySep Human Monocyte Enrichment Kit (STEMCELL Technologies ...
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bioRxiv - Microbiology 2019Quote: ... freshly isolated PBMCs obtained from healthy donor peripheral blood (lymphocyte separation media; StemCell Technologies) were subjected to CD14+ magnetic bead positive selection using the MojoSort Human CD14 Selection Kit (BioLegend) ...
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bioRxiv - Microbiology 2019Quote: Freshly isolated PBMCs obtained from healthy donor peripheral blood (lymphocyte separation media; StemCell Technologies) were subjected to CD4 or CD8 T cell magnetic bead negative selection using the MojoSort Human CD4 or CD8 Selection Kit (BioLegend) ...
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bioRxiv - Cell Biology 2020Quote: ... Primary NK cell subsets from blood were enriched with NK cell RosetteSep (StemCell Technologies). Blood was layered onto Ficoll-Paque density gradient followed by centrifugation at 2,000 rpm for 20 mins (no brake) ...
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bioRxiv - Cell Biology 2020Quote: ... Diluted blood (20 ml) was layered on to 15 ml of Lymphoprep (Stemcell Technologies) in 50-ml Falcon tubes and centrifuged at 400× g for 30 min without deceleration ...
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bioRxiv - Cancer Biology 2021Quote: ... Blood-soaked tissue samples were washed with an ammonium chloride solution (Stemcell Technologies, Canada) to lyse present erythrocytes ...
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bioRxiv - Immunology 2020Quote: Peripheral blood mononuclear cells (PBMCs) were isolated with gradient centrifugation using Lymphoprep (StemCell Technologies) and the red blood cells were removed by inclubating the cells with RBC lysis buffer (Tonbo Biosciences) ...
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bioRxiv - Cell Biology 2022Quote: ... Peripheral blood mononuclear cells were isolated using the density gradient medium Lymphoprep (StemCell Technologies). Primary isolated PBMCs were Cell Tracker Green (CTG ...
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bioRxiv - Immunology 2023Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated through gradient centrifugation using Lymphoprep (STEMCELL Technologies) within 4 hours ...
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bioRxiv - Immunology 2023Quote: ... PBMCs were isolated from blood via density centrifugation using Lymphoprep (StemCell Technologies Cat #07801). The interphase was collected and red blood cells lysed (155 mM NH4CL ...
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bioRxiv - Immunology 2023Quote: PBMCs were isolated from the peripheral blood of healthy donors using Lymphoprep (Stemcell Technologies), according to the manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated through Lymphoprep density gradient separation (Stemcell Technologies). Cells were used after cryopreservation ...
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bioRxiv - Immunology 2023Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated using Lymphoprep™ (07811, StemCell Technologies, Canada) and SeptMate™-50 PBMC Isolation Tubes (85460 ...
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bioRxiv - Cancer Biology 2023Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated through gradient centrifugation using Lymphoprep (STEMCELL Technologies) within 4 hours of blood collection ...
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bioRxiv - Cancer Biology 2024Quote: Peripheral blood mononuclear cells (PBMCs) from healthy donors were obtained commercially (Stem Cell Technologies). For in vitro macrophage polarization experiments ...
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bioRxiv - Cancer Biology 2024Quote: ... Red blood cells (RBCs) were lysed with 0.8% ammonium chloride solution (StemCell Technologies, 07850). Epithelial organoids were further digested by 0.05% pre-warmed Trypsin (Life Technologies ...
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bioRxiv - Genomics 2024Quote: Peripheral blood mononuclear cells (PBMCs) were isolated using RosetteSep Granulocyte Depletion Cocktail (StemCell Technologies), following manufacturer’s protocols for density gradient centrifugation ...
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bioRxiv - Microbiology 2021Quote: ... were generated by positive selection of CD14+ cells from PBMCs using the EasySep Human CD14 Positive Selection Kit II (STEMCELL Technologies, Cat: 17858) and differentiating cells for at least 7 days in RPMI supplemented with 10% Human AB Serum (Millipore Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... we first isolated CD4+ T cells from 10M PBMC by negative selection using the EasySep Human CD4+ T cell Enrichment Kit (STEMCELL Technologies, Cat #: 19052). This yielded a mean of 1.6 (range 1.3-2.0 ...
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bioRxiv - Bioengineering 2020Quote: ... PBMCs were further purified for CD3+ cells by immunomagnetic negative selection using the EasySep Human T-cell Isolation Kit (STEMCELL Technologies, Cambridge, MA). T cells were frozen at 10–20 × 106 cells per 1 mL of Cryostor CS10 (STEMCELL Technologies ...
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bioRxiv - Cancer Biology 2019Quote: ... 1×107/ml of PBMCs were used for isolation of CD4+ CD127low CD25+ Regulatory T cells using the EasySep™ CD4+ CD127low CD25+ Human Regulatory T Cell Isolation Kit (STEMCELL Technologies, MA) following the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2019Quote: ... Cells were first enriched for CD8 T cells using EasySep™ human total or naïve CD8 T Cell enrichment kits (STEMCELL Technologies, Vancouver, Canada). CD56− and CD56+ PBMC fractions were sorted by flow cytometry ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T cells were then isolated from the cell layer using the EasySep™ human CD4+ T cell isolation kit (StemCell Technologies #17952), according to manufacturers’ instructions.
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bioRxiv - Immunology 2020Quote: ... human peripheral blood monocytes were isolated from freshly prepared PBMCs taken from healthy donors using an EasySep™ Human Monocyte Enrichment Kit (negative selection cell; StemCell Technologies, Canada). Cells were incubated in CTL medium supplemented with 100 U/ml Penicillin-Streptomycin ...
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bioRxiv - Microbiology 2023Quote: ... Cells were counted and CD19+ B cells were isolated using the EasySep Human Pan-B cell enrichment kit (Cat# 19554, StemCell Technologies, Vancouver, Canada) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... naïve CD4+ T-cells were isolated via two-stage negative selection with EasySep™ Human Naïve CD4+ T-Cell Isolation Kit II (Stemcell Technologies Cat# 17555); and CD8+ T-cells were isolated by negative selection from PBMCs with Stem Cell Technology Easy Sep CD8+ kit ...
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bioRxiv - Immunology 2023Quote: ... CD34+ human hematopoietic stem/progenitor cells (hHCs) were isolated using the EasySep™ Human CD34 positive selection kit according to manufacturer’s instructions (StemCell Technology, Vancouver, Canada). These cells were cultured ex vivo using various protocols ...
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bioRxiv - Microbiology 2023Quote: ... CD4+ T cells were isolated from fresh or frozen PBMCs by negative selection using the EasySep™ human CD4+ T cell isolation kit according to the manufacturer’s instructions (Stem Cell Technology, USA), and activated with CD2/CD3/CD28 magnetic beads (Miltenyi Biotec ...
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bioRxiv - Genomics 2023Quote: ... B cells were purified from PBMC by negative selection using the StemSep™ Human B Cell Enrichment Kit (STEMCELL Technologies Inc., Vancouver, Canada) following the manufacturers’ instructions.
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bioRxiv - Cancer Biology 2023Quote: ... Primary NK cells were isolated from PBMCs by immunomagnetic negative selection using EasySep™ Human NK Cell Enrichment Kit (STEMCELL Technologies Canada, Inc.) according to the manufacturer’s protocols ...
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bioRxiv - Genomics 2023Quote: ... The washed PBMCs were resuspended at 200E6 cells/mL in EasySep buffer and isolated with the EasySep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit (STEMCELL Technologies, #18063), according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were suspended in Hanks Balanced Salt Solution (HBSS) supplemented with 5% human serum and 1.5 µg/mL anti-human CD32 antibody (Clone IV.3; STEMCELL Technologies) and then stained with designated antibodies for 1-2 hours on ice prior to sorting on a Becton Dickinson FACSAria™ Fusion or FACSAria™ III sorter ...
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bioRxiv - Bioengineering 2021Quote: ... Primary human T cells were isolated using negative selection per the manufacturer’s instructions (RosetteSep Human T Cell Enrichment Cocktail, STEMCELL Technologies). T cells were counted using a Countess II FL Automated Cell Counter (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... total PBMCs or purified human naïve CD4+ T cells were stimulated with the ImmunoCult human T cell activator (StemCell Technologies) in the presence of either DMSO or soquelitinib for 72 hours ...
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bioRxiv - Cancer Biology 2022Quote: TS543 human glioma cell line was cultured in human NeuroCult™ NS-A Basal Medium (Stem Cell Technologies, cat #05751), supplemented with 10% NeuroCult™ Proliferation Supplement ...
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bioRxiv - Immunology 2022Quote: ... recombinant human FGF (25ug/mL; Stem Cell Technologies), and recombinant human EGF (10 ug/mL ...
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bioRxiv - Immunology 2020Quote: ... Human Recombinant IFNγ (10ng/mL, StemCell Technologies 78020) was added to the media for a period of 60 minutes ...
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BCR analysis of single-sorted, putative IgE+ memory B cells in food allergy: an ephemeral existence?bioRxiv - Immunology 2019Quote: ... or anti-human CD32 (FcγRII Blocker, StemCell Technologies) for 15 minutes on ice to block non-specific staining and then incubated with fluorochrome-conjugated antibodies for 30 minutes on ice and protected from light ...
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bioRxiv - Microbiology 2020Quote: ... and RosetteSep Human CD8 Depletion Cocktail (Stemcell Technologies) per the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... recombinant human IL-12 (Stemcell Technologies, Vancouver, Canada), IL-15 (Miltenyi Biotec ...
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Single-cell profiling and zebrafish avatars reveal LGALS1 as immunomodulating target in glioblastomabioRxiv - Cancer Biology 2023Quote: ... recombinant EGF (20 ng/mL, human, STEMCELL Technologies) and antibiotic antimycotic solution (100x ...
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bioRxiv - Cell Biology 2023Quote: Frozen human PBMCs were purchased from STEMCELL Technologies. After thawing by immediately placing them into a 37°C water bath and transferring them into a pre-warmed RPMI1640 with 10% FBS and 1% Penicillin-Streptomycin ...
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bioRxiv - Immunology 2023Quote: ... frozen human and cynomolgus macaques PBMCs (Stemcell Technologies, Cat# 70025.2 and iQ Biosciences ...
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bioRxiv - Cell Biology 2023Quote: Frozen human PBMCs were purchased from STEMCELL Technologies, and dead PBMCs were prepared by treating them with 70% ethanol for 30 min ...
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bioRxiv - Cancer Biology 2023Quote: ... recombinant human Noggin (100 ng/ml, Stemcell Technologies), B-27 supplement (ThermoFisher) ...