Labshake search
Citations for Stemcell Technologies :
601 - 650 of 1493 citations for 3 Piperidinol 1 methyl 4 2 4 6 trimethoxyphenyl cis + since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... cells were seeded at 5000 cells/ml in 3 ml MethoCultTM (Stem Cell Technologies, H4034) in a 3.5 cm culture dish and placed in an incubator at 37 °C and 5% CO2 ...
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bioRxiv - Developmental Biology 2023Quote: ... Explants were then cultured in suspension in DMEM for 2 hours at 37°C with a 1/250 dilution from 100 mM stock solution or Dispase II (Stem Cell Technologies; #07923, at 0.2 U/mL).
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bioRxiv - Immunology 2021Quote: ... (2) ImmunoCult™-XF T Cell Expansion Medium (STEMCELL Technologies) was supplemented with 1X Penicillin Streptomycin solution (ICM) ...
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bioRxiv - Biophysics 2024Quote: ... ovaries were treated with collagenase (2 mg/mL; StemCell Technologies) and incubated in Modified Bath Saline (MBS ...
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bioRxiv - Bioengineering 2024Quote: ... and 2 mg/mL dispase II (STEMCELL Technologies, Vancouver, Canada) for 4 hours under gentle agitation at 37° C ...
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bioRxiv - Microbiology 2023Quote: ... and Heparin solution (2 μl/ml; Stem cell technologies #07980).
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 2 µM Thiazovivin (StemCell Technologies, Cambridge, MA 02142) and plated in a Matrigel-coated 12-well plate at a density of 3 x 105 cells per well ...
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bioRxiv - Cancer Biology 2023Quote: ... heparin solution (final concentration, 2 µg/mL, STEMCELL Technologies, 07980), recombinant human EGF (20 ng/mL ...
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bioRxiv - Cell Biology 2023Quote: ... for EB formation in APEL-2 media (Stemcell Technologies, 5275) containing 10µM ROCK inhibitor ...
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bioRxiv - Neuroscience 2024Quote: ... in 2 ml EB Induction Medium (mTeSR+ (Stem Cell Technologies) + 20 ng/ml SCF (R & D Systems ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 2% NeuroCult™ SM1 neuronal supplement (STEMCELL Technologies) at a temperature of 37 °C and 5% CO2 ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 2% NeuroCultTM SM1 neuronal supplement (STEMCELL Technologies, 05711) and 2 mM GlutaMAX™ Supplement (Gibco ...
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bioRxiv - Cell Biology 2024Quote: ... and 2× cell culture medium (MesencultTM ACF Plus (Stemcell Technologies) with 4 mM GlutaMAX-ITM (Gibco ...
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Genetically engineered microglia-like cells have therapeutic potential for neurodegenerative diseasebioRxiv - Neuroscience 2021Quote: ... lineage negative cells were enriched from total bone marrow isolated from femurs and tibias of donor mice (6-12 weeks of age) using the EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (STEMCELL Technologies) in conjunction with the RoboSep-S (STEMCELL Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... neural tissues were embedded in Matrigel droplets and moved to ultralow attachment 6-well plates and fed expansion media (Stemcell Technologies). Then at day 10 ...
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bioRxiv - Genomics 2021Quote: ... on Costar non-treated 6 well polystyrene plates (Catalog Number: 3736; Lot Number: 30417038) coated with Vitronectin XF (Stem Cell Technologies; Catalog Number ...
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bioRxiv - Microbiology 2021Quote: ... neural tissues were embedded in Matrigel droplets and moved to ultralow attachment 6-well plates and fed expansion media (Stemcell Technologies). Day 10 organoids were transferred to a rocker to elicit the circulation of nutrients and prevent organoids from sticking to the dish ...
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bioRxiv - Immunology 2022Quote: ... diluted in MethoCult GF-M3434 medium and plated at 20,000 cells/35 mm well in a 6-well plate of low-adherence culture-ware (STEMCELL Technologies, Inc.), in triplicate ...
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bioRxiv - Immunology 2022Quote: Neutrophils were obtained from the bone marrow of C57BL/6 wild-type mice using the EasySep Mouse Enrichment Kit (STEMCELL Technologies), following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Harvested spheroids then were transferred into 10-cm ultra-low adhesion plates in Essential 6 medium containing dual SMAD pathway inhibitors [100 nM LDN193189 (STEMCELL Technologies) and 10 μM SB431542] and 2.5 μM XAV939 (Cayman) ...
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bioRxiv - Neuroscience 2022Quote: ... the dissociated cells were transferred to individual low-adherence V-bottom 96-well plates (S-Bio Prime) in 150μl TeSR5/6 media (StemCell Technologies®) with 50μM Rock inhibitor Y-27632 (Tocris BioScience) ...
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bioRxiv - Cell Biology 2022Quote: ... and cultured in new 6-well plate using mTeSR™ plus medium supplemented with 10 μM Y-27,632 Rock inhibitor (StemCell Technologies). Pluripotency of all lines was established by analyzing the expressions of canonical stemness genes (SOX2 ...
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bioRxiv - Genetics 2022Quote: ... and harvested and replated on day 6 on matrix-coated tissue culture plates and cultivated for 7 days in STEMdiffTM Neural Induction Medium (Stemcell Technologies) with daily medium changes ...
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bioRxiv - Neuroscience 2021Quote: ... Nucleofected cells were transferred to a new matrigel-coated well in a 6-well plate in 4mL of E8 medium containing 10μM ROCK inhibitor Y-27632 (StemCell Technologies #72307). Medium was replaced the following day with fresh E8 ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were gently resuspended in media and transferred into 6-well plates in mTeSR Plus media containing CloneR2 reagent (STEMCELL Technologies) for AAVS1 targeting ...
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bioRxiv - Cancer Biology 2024Quote: ... pan CD3+ murine T cells were isolated from single cell splenocyte suspensions of 6 – 12-week-old mice through a negative selection process (STEMCELL Technologies). T cells were activated with αCD3/αCD28 Dynabeads as specified by manufacturer’s instructions in the presence of 100 IU/mL recombinant mouse (rm ...
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bioRxiv - Molecular Biology 2022Quote: ... T cells were seeded at 2 × 106 cells/mL (typically 10×106 cells per well of a 6-well plate) and activated with ImmunoCult T Cell activator (STEMCELL Technologies) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Double-positive cells were plated into a matrigel-coated 6 cm petri dish in mTeSR supplemented with 1x CloneR (Stemcell Technologies). Individual colonies were manually picked into 96 wells ∼10 days after transfection ...
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bioRxiv - Bioengineering 2023Quote: ... gift from the Seidman Lab) or GFP-DSP reporter (WTC; Allen Institute AICS-0017 cl.6) were cultured in mTeSR1 media (StemCell Technologies) on Matrigel (Corning ...
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bioRxiv - Cell Biology 2024Quote: ... the media was changed to remove the ROCK inhibitor and was maintained for 6 hours in in mTeSR plus (Cat# 100-0276, StemCell Technologies). The media was then replaced with DMEM/F12 (Cat# 11320033 ...
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bioRxiv - Microbiology 2024Quote: ... We initiated the organoid development by plating hiPSCs at density 20- 30% in TeSR1 medium on a 6-well plate coated with human embryonic stem cell (hESC)-qualified basement membrane matrix (STEMCELL Technologies). The next day ...
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bioRxiv - Bioengineering 2024Quote: Our protocol began with cultivating human pluripotent stem cells (H9) in a Matrigel-coated 6-well plate using a stem cell feeder-free culture medium (mTeSR1, STEMCELL Technologies). For routine passaging ...
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bioRxiv - Cell Biology 2024Quote: ... The microglial differentiation was performed on thawed HPCs (200,000 cells per well of a 6-well plate) using the STEMdiffTM Microglia Culture System (cat. 100-0019 and 100-0020, STEMCELL Technologies) for 24 days according to manufacturer’s directions ...
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bioRxiv - Microbiology 2020Quote: ... the induced hAFECs was cultured with the SFM medium supplemented with 3 μM CHIR99021 (STEMCELL Technologies), 10 ng/ml BMP4 (Peprotech) ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were passaged every 3-5 days after dissociation with ReLeSR(Stem Cell Technologies, 100-0483). The medium was supplemented with Rho-associated protein kinase (ROCK ...
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Self-organization of embryonic stem cells into a reproducible embryo model through epigenome editingbioRxiv - Developmental Biology 2024Quote: ... complete solution was removed and 3 ml 2i+Lif medium + 10uM ROCK inhibitor (Stemcell Technologies, 72304) was added ...
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bioRxiv - Developmental Biology 2023Quote: ... 3 million cells were added to one well of an Aggrewell™800 (StemCell Tech, 34815) with neural induction media ...
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bioRxiv - Bioengineering 2023Quote: ... Stages 3-7 used basal media of DMEM supplemented with BSA and SM1 (Stem Cell Technology) or B27 (ThermoFisher) ...
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bioRxiv - Developmental Biology 2024Quote: ... culture medium is switched to E6 medium containing 3 µM CHIR99021 (STEMCELL Technologies, cat # 100-1042), 25 ng mL-1 BMP4 and 50 ng mL-1 FGF2 ...
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bioRxiv - Cell Biology 2020Quote: ... cells in a 6 well plate were washed with 1X PBS then subjected to 2.5 units/ml of dispase (Stemcell Technologies, Kent, WA). The plate was placed on an orbital shaker to induce mechanical stress at room temperature ...
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bioRxiv - Immunology 2021Quote: OVA (SIINFEKL)-specific CD8+ T cells were isolated from the spleen and lymph nodes of healthy 6-8-week old OT-I mice using EasySep™ Mouse CD8+ T-Cell Isolation Kit (STEMCELL Technologies) according to the manufacturer’s protocols ...
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bioRxiv - Bioengineering 2021Quote: ... hiPSCs were thawed into 6 well plates (TPP) that were pre-coated for 1h at RT with 10 μg/ml Vitronectin (Stem Cell Technologies) in CellAdhere dilution buffer (Stem Cell Technologies) ...
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bioRxiv - Cancer Biology 2020Quote: ... Appropriate numbers of total bone marrow cells were then exposed to various concentration of MMC and seeded into 6-well plates with MethoCult GF M3434 (Stem Cell Technologies) media following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: ... were harvested from 6-12 weeks old in-house bred BL/6 mice (WT or transgenic CD2 Cre/CD4 Cre mice) using the EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (Stem Cell Technologies). HSPCs were transduced with retrovirus for expression of the desired oncogenes ...
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bioRxiv - Immunology 2022Quote: Untouched PMNs were isolated from bone marrow in donor mice of the indicated genotype (age 6–8 wk) using EasySep mouse neutrophil enrichment kit (Stemcell Technologies Inc.). In a subset of experiment PMNs from C57/J animals were incubated with 1 mM Dimethyloxalylglycine (DMOG ...
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bioRxiv - Cell Biology 2021Quote: ... 288 mio NPCs per axis were aggregated into 14400 organoids (20000 cells/per organoid) in Aggrewell800 6-well plates (Stem Cell Technologies). The day after ...
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bioRxiv - Immunology 2020Quote: ... C57BL/6 mice by use of magnetic separation with the EasySep™ Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (STEMCELL Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... a piece of frozen tumor tissue was placed into a well of a 6-well plate (Stem cell Technologies, catalog no. 38015) with 1 mL of either CST ...
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bioRxiv - Bioengineering 2021Quote: ... 1.5×10^6 cells were used for each targeting and were plated onto geltrex coated wells on 6-well plates in mTeSR with CloneR (Stemcell Technologies, 05889) following nucleofection ...
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bioRxiv - Genetics 2020Quote: ... emerging iPSC colonies that were large enough to isolate were manually picked using a sterile pulled glass pipette into VTN-coated 6-well tissue culture plates containing complete TeSR-E8 media (TeSR-E8 basal medium with 25X TeSR-E8 supplement) (Stem Cell Technologies) using a SMZ1000 Nikon dissecting microscope (Nikon ...