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Citations for Stemcell Technologies :
551 - 600 of 838 citations for tert Butyl 3 5 2 amino ethyl thiophen 3 yl propionate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... and passaged by dissociating to single cells (5-minute treatment with 1X Accutase at 37 °C; STEMCELL Technologies) and resuspending at a concentration of 100k-300k cells/mL.
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bioRxiv - Developmental Biology 2020Quote: ... and pluripotent stem cells were passaged every 5 to 7 days in the ReLeSR medium (Stem Cell Technologies). Pluripotent iPSC and hESC were differentiated into retinal organoids as previously described (9 and Fig S1 ...
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bioRxiv - Developmental Biology 2020Quote: ... 10ng/ml bFGF and 5 ng/ml BMP4 and plated in ultra-low attachment plates (Stem Cell Technologies). Two days later ...
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bioRxiv - Neuroscience 2022Quote: ... 5 min) and plated at a density of 90,000 cells/mL in STEMdiff Neural Induction Medium (StemCell Technologies) with SMADi supplement (StemCell Technologies ...
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bioRxiv - Neuroscience 2022Quote: ... on Matrigel-coated culture dishes at 37°C and 5% CO2 and passaged using ReLeSR (Stem Cell technologies). CRX-mCHERRY hiPSCs were grown on vitronectin (VTN-N ...
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bioRxiv - Cell Biology 2022Quote: ... at 37°C for 5 min and resuspended in E8 plus 10 µM Y-27632 (Stem Cell Technologies) and seeded into AggreWell 800 plates (StemCell Technologies ...
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bioRxiv - Cell Biology 2024Quote: ... at 37°C for 5 min and resuspended in E8 plus 10 µM Y-27632 (Stem Cell Technologies) and seeded into AggreWell 800 plates (StemCell Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were subjected to red cell lysis in 5 ml ammonium chloride lysis buffer solution (Stem Cell Technologies) on ice for 10 minutes ...
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Efficient Methods for Target Gene Manipulation in Haematopoietic Stem Cell Derived Human NeutrophilsbioRxiv - Cell Biology 2023Quote: ... Cells were plated at a minimum density of 5 × 105 cells/mL in SFEM II medium (StemCell Technologies) with StemSpanTM CD34+ Expansion Supplement (StemCell Technologies) ...
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bioRxiv - Developmental Biology 2023Quote: ... and kept on Rho-associated protein kinase (ROCK) inhibitor Y-27632 (final concentration 5 μM, StemCell Technologies, #72302) for one day ...
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bioRxiv - Cell Biology 2024Quote: ... This was followed by cardiac specification using RPMI B27-medium containing 5 µM IWP-4 (Stem Cell Technologies) for another 3 days ...
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bioRxiv - Molecular Biology 2024Quote: ... iPSCs were maintained at 37 °C and 5% CO2 in mTeSR or mTeSR plus medium (Stem Cell Technologies) on Geltrex-coated (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... Approximately 1×10^5 cell per ml were plated in triplicate into M3234 incomplete methylcellulose medium (StemCell Technologies) supplemented with 0.1 ng/mL GM-CSF (Peprotech) ...
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bioRxiv - Cell Biology 2024Quote: ... Purified human CB CD34+ HSPC were isolated and cultured at 5×105/mL in StemSpan SFEMII (Stemcell Technologies) supplemented with human stem cell factor (150ng/mL) ...
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bioRxiv - Genomics 2020Quote: ... Tissues were minced into ~1-2 mm pieces and digested with 1× Collagenase/Hyaluronidase (STEMCELL Technologies) and 100 μg/mL DNase I (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2020Quote: ... Dual SMAD inhibitors (2μM Dorsomorphin; StemCell Tech; #72102, and 2 μM A83-01; StemCell Tech; #72022) until day 5 ...
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bioRxiv - Neuroscience 2021Quote: ... with the addition of the following supplements 2% (v/v) NeuroCultTM SM1 Neuronal Supplement (StemCell Technologies), 1% (v/v ...
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bioRxiv - Genetics 2022Quote: ... neuronal differentiation media was switched after 2 weeks to BrainPhys™ Neuronal Medium (Stem Cell Technologies) supplemented with the same factors to promote optimal neuronal activity and maturation.
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bioRxiv - Developmental Biology 2022Quote: ... Half well equivalent was seeded into 2 ml of methylcellulose medium (Human enriched H4435, Stemcell Technologies). Cells were incubated in the assay for 14 days and then scored.
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bioRxiv - Neuroscience 2022Quote: ... were maintained in serum-free STEMdiff™ Neural Progenitor Medium 2 (Stem Cell Tech cat#08560) on Corning® Matrigel® hESC-qualified Matrix (Corning cat#354277 ...
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bioRxiv - Genomics 2021Quote: Tissues were minced into ~1-2 mm pieces and digested with 1× Collagenase/Hyaluronidase (STEMCELL Technologies) and 100 μg/mL DNase I (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... C3H10T1/2 cells and MSCs were treated with Mesencult ™ Osteogenic Stimulatory Kit (STEMCELL TECHNOLOGIES, #05504).
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bioRxiv - Neuroscience 2022Quote: ... on day 2 media was changed to Neurobasal A and DMEM/F12 1:1 (Stemcell technologies) supplemented with N2 0.5% ...
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bioRxiv - Developmental Biology 2023Quote: ... with 6 μM CHIR99021 in 1.2 mL STEMdiff™ APEL™2 medium (STEMCELL Technologies, # 05270) at the bottom of the chamber for 1-hour ...
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bioRxiv - Genetics 2022Quote: ... HUDEP-2 cells were expanded in a basal medium composed of StemSpan SFEM (Stem Cell Technologies) supplemented with 10−6 M dexamethasone (Sigma) ...
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bioRxiv - Developmental Biology 2023Quote: ... The following day the medium was replaced with STEMdiff™ APEL™2 (StemCell Technologies, #05270), containing 8 μM CHIR-99021 (Tocris ...
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bioRxiv - Cell Biology 2023Quote: ... medium was changed to 2 mL of complete STEMdiffTM Astrocyte Differentiation Medium (ADM) (Stemcell Technologies, #08540). On day7 and day 14 ...
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bioRxiv - Bioengineering 2024Quote: ... hPSCs designated for animal experiments were maintained in xeno-free TeSRTM 2 media (Stemcell Technologies, #05860). Cells were split every 5-6 days using TrypLE Express (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... 10 ng/mL Human Recombinant bFGF) and 2 μg/mL Heparin Solution (Stemcell Technologies Canada Inc.). For a subset of experiments neurospheres were moved to Complete Medium (CM ...
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bioRxiv - Neuroscience 2024Quote: ... Approximately 2 × 106 single cells were added per AggreWell800 well in mTeSR PLUS medium (STEMCELL technologies) supplemented with 20µM ROCK inhibitor Y-27632 (STEMCELL technologies) ...
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bioRxiv - Developmental Biology 2024Quote: ... in 2 ml EB Medium A supplemented with 10 µM ROCK inhibitor Y-27632 (STEMCELL Technologies) and incubated at 37°C ...
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bioRxiv - Bioengineering 2024Quote: ... the media was switched to RPMI 1640 medium supplemented with 5µM IWP-2 (72124, Stemcell technologies) to inhibit the Wnt pathway with daily media changes for another 48h ...
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bioRxiv - Synthetic Biology 2024Quote: ... the medium was further supplemented with 100 U/mL recombinant human IL-2 (STEMCELL technologies, Germany). Blood samples were provided by the Institute for Clinical Hemostaseology and Transfusion Medicine ...
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bioRxiv - Cancer Biology 2024Quote: ... and cultured for 1 day in the presence of 100 units of IL-2 (StemCell Technologies) followed by 2 days in T cell medium alone ...
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bioRxiv - Neuroscience 2020Quote: ... Primary mouse microglia and M-MØP cells were exposed at set time points to anti-FcγRII/III (2.4G2) antibody at 5 µg/ml (Stemcell technologies), LPS (50 ng/ml ...
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bioRxiv - Cell Biology 2022Quote: ... mESCs were cultured in feeder free conditions with 5% CO2 at 37°C on 0.1% gelatin (StemCell Technologies, 7903) coated plates in 2i medium supplemented with 2% mES certified FBS (Gibco 16141061) ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were seeded at 5 × 105 cells/ml and grown in Neurocult mouse neurobasal medium (Stem Cell Technologies, 5700) supplemented with 10 ng/ml hEGF (Lonza ...
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bioRxiv - Microbiology 2020Quote: ... 5×106 naïve CD4+ T cells from HIV− or HIV+ donors were isolated by magnetic negative selection (StemCell Technologies), then cultured at 106 cells/mL in 96-well plates using R-10 media (RPMI-1640 media supplemented with 10% FBS ...
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bioRxiv - Bioengineering 2021Quote: ... coated tissue culture plates and passaged regularly as cell aggregates every 4-5 days using ReLeSR (Stemcell Technologies, 05872).
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bioRxiv - Molecular Biology 2020Quote: ... CD34+ HSPCs were cultured at 2.5×105–5×105 cells/mL in StemSpan SFEM II (STEMCELL Technologies, Vancouver, Canada) base medium supplemented with stem cell factor (SCF)(100ng/mL) ...
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bioRxiv - Cancer Biology 2022Quote: ... We grew cells in a 5% CO2 incubator in a serum-free NeuroCult NS-A basal medium (STEMCELL Technologies) supplemented with NeuroCult™ Proliferation Supplement (STEMCELL Technologies) ...
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bioRxiv - Bioengineering 2022Quote: iPSC lines were maintained and expanded at 37°C in 5% CO2 in mTeSR™Plus medium (Stemcell Technologies) on 1:100 diluted Geltrex (Thermo Fisher)-coated plates with daily medium changes and passaging every 3-4 days with releasing agent ReLeSR (Stemcell Technologies).
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bioRxiv - Cell Biology 2022Quote: ... Red blood cells were removed using the ammonium chloride solution: 5 ml of 0.8% Ammonium Chloride (StemCell Tech. 07800) was added to the suspension and incubated for 10 min with intermittent mixing ...
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bioRxiv - Developmental Biology 2022Quote: ... Dispase II at 5 U/ml stock solution in Hanks’ balanced saline was from Stemcell Technologies (cat. no. 07913). The following chemicals were prepared and used according to the manufacturers’ guidelines ...
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bioRxiv - Cell Biology 2024Quote: ... mesoderm was carried out for 5 days and ectoderm for 7 days using STEMdiff Trilineage Differentiation Kit (STEMCELL Technologies). Alternatively ...
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bioRxiv - Neuroscience 2023Quote: ... the hiNPCs were singularized with Accutase for 10 min at 37 °C and 5 % CO2 (#07920, Stemcell Technologies, Canada) and centrifuged (300 g ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 x 105 CD3+ T cells were plated in 0.5 ml T cell expansion medium (Stemcell Technologies, Cologne, Germany) with 50 IE/ml IL-2 ...
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bioRxiv - Immunology 2023Quote: ... Cells were pelleted at 300g for 5 mins and RBC lysed using Ammonium Chloride Lysis Buffer (Stem Cell Technologies) as per manufacturer protocol ...
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bioRxiv - Cell Biology 2023Quote: ... 1% (v/v) B27™ minus insulin) supplemented with 5 µM CHIR99021 and 33.3 ng Activin A (STEMCELL Technologies). Medium was exchanged daily without CHIR99021 supplementation ...
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bioRxiv - Cell Biology 2023Quote: ... After 5 weeks the H5100 medium was replaced with Methocult H4434 Classic methylcellulose medium (100µl/ well, STEM CELL technologies). Wells that contained at least one colony after 10-14 days were considered positive.