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Citations for Stemcell Technologies :
551 - 600 of 614 citations for Transcription Factor SOX 10 SOX10 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: CD34+ HPCs isolated from human bone marrow were infected at an MOI of 2 for 20h in IMDM supplemented with 10% BIT9500 serum substitute (Stem Cell Technologies, Canada), 2 mM L-Glutamine ...
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bioRxiv - Cell Biology 2021Quote: ... to disperse into single cells and replated in mTeSR1 supplemented with 1% P/S containing 10 µM Rock Inhibitor (Y-27632, Stem Cell Technologies #72308). A full protocol is available at the Allen Cell Explorer (www.allencell.org/methods-for-cells-in-the-lab SOP ...
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bioRxiv - Cancer Biology 2021Quote: Metastatic PDX tissues that had been frozen down in either FBS containing 10% dimethyl sulfoxide (DMSO) or primary PDX tissues that were frozen in neuronal stem cell medium (NSC medium, Stemcell technologies, Köln, Germany) containing 10% DMSO were thawed by brief incubation at 37°C and were transferred to basal NSC medium ...
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bioRxiv - Bioengineering 2022Quote: ... until a final volume of 200 ml and layered per 25 ml on top of 10 ml LymphoprepTM (07851, StemCell technologies, Köln, Germany) in 50 ml centrifugal tubes ...
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bioRxiv - Developmental Biology 2022Quote: ... and 1 × 106 cells were seeded per well of an Aggrewell™800 plate in APEL2 medium supplemented with 10 μM Y-27632 ROCK inhibitor (#72307, StemCell Technologies Inc.) followed by 72h incubation with daily media change ...
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bioRxiv - Neuroscience 2024Quote: ... media was replaced every other day with neuronal differentiation media prepared based on the Stem Cell Technology protocol: 10 ml of the BrainPhys™ Neuronal Medium (#05790; Stem Cell Technology) supplemented with 200 µl of NeuroCultTM SM1 Neuronal Supplement (#05711 ...
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bioRxiv - Immunology 2024Quote: ... PBMCs were isolated from whole blood using density gradient centrifugation at 1,200 x g for 10 min where diluted blood was layered over 15 mL of Lymphoprep™ (STEMCELL Technologies, Vancouver, Canada) followed by harvesting of the buffy coat ...
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bioRxiv - Cancer Biology 2024Quote: ... Corning 10-092-CV; 20% fetal bovine serum [FBS], Sigma-Aldrich F4135; and 0.1 mg ml-1 DNase I, Stem Cell Technologies 07900). Cell suspension was placed in a new tube ...
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bioRxiv - Cell Biology 2023Quote: ... buffy coat-PBS-citrate solution was added in portions of 25 ml to tubes filled with 10 ml LymphoprepTM (07851, StemCell technologies, Köln, Germany). Tubes were centrifuged (20 min at RT ...
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bioRxiv - Cell Biology 2023Quote: ... 3.3 x 105 cells and 2.6 x 105 cells per ml were seeded in 3 ml E8 medium supplemented with 10 µM Rho-associated coiled-coil kinase (ROCK) inhibitor Y-27632 (RI, STEMCELL Technologies, Vancouver, Canada), respectively ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 2% (v/v) B27™ minus insulin and 1% (v/v) GlutaMax™) supplemented with SB431542 (10 µM) and Dorsomorphin (1 µM, both STEMCELL Technologies). Medium was replaced daily ...
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bioRxiv - Cancer Biology 2023Quote: ... Pellets were then suspended with 1 ml pre-warmed to 37°C A-mTE-D2 (7 mg ml-1 Dispase II, Worthington NPRO2, and 10 µg ml-1 Deoxyribonuclease I, Stem Cell Technologies, 07900), and mixed 70 times with a 1000 µl pipette tip ...
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bioRxiv - Neuroscience 2024Quote: Frozen DIV 4 stock of NGN2 iPSC-derived cortical neurons (Brigham & Women’s Hospital Neuro iHub) were gently thawed and resuspended in NB-B27 media with RHO/ROCK pathway inhibitor (RI, 10 μM, Stemcell Technologies, Y-27632). A small volume (10 μL ...
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bioRxiv - Cell Biology 2024Quote: ... and then re-seeded in supplemented mTeSR1 medium with 1% penicillin–streptomycin and a 10 μM concentration of the ROCK inhibitor Y-27632 (Stem Cell Technologies, 72308). Further details for cell culture reagents and consumables can be found in the Resource Table and standard protocols can be found at www.allencell.org.
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bioRxiv - Cancer Biology 2024Quote: ... NK cells were stained with CellTrackerTM Green CMFDA (5-chloromethylfluorescein diacetate) (InvitrogenTM C7025) diluted to 10 µM in serum free ImmunoCultTM NK Cell Base Medium (STEMCELL Technologies 100-0712) for 30 min ...
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bioRxiv - Developmental Biology 2024Quote: ... the cells were plated in 6-well plates using mTeSR media supplemented with 10 µM Y-27632 (Stem Cell Technologies, catalog no. 72304) for 48 hours ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were suspended in Hanks Balanced Salt Solution (HBSS) supplemented with 5% human serum and 1.5 µg/mL anti-human CD32 antibody (Clone IV.3; STEMCELL Technologies) and then stained with designated antibodies for 1-2 hours on ice prior to sorting on a Becton Dickinson FACSAria™ Fusion or FACSAria™ III sorter ...
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bioRxiv - Immunology 2019Quote: ... LV-CAR T cells were expanded by stimulation with an antibody cocktail (anti-CD3/CD28; STEMCELL Technologies; cat. no. 10971) combined with IL-2 (50 U/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were incubated with a cocktail of lineage specific antibodies (CD5, CD11b, CD19, B220, Gr-1 and TER119, Stemcell Technologies) for negative selection of Lin-cells using an EasyStep immunomagnetic column (Stemcell Technologies) ...
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bioRxiv - Bioengineering 2022Quote: ... Antibody-bound cells were depleted with streptavidin RapidSphere™ magnetic beads according to the manufacturer’s instructions (STEMCELL Technologies; Vancouver, Canada). Cells with >90% purity were used for experiments ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were harvested and blocked with CD32 antibody (2 µl per 50,000 cells in 100 µL, STEMCELL Technologies Catalog #60012) for 15 minutes at 4C ...
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bioRxiv - Bioengineering 2023Quote: ... The samples were then incubated overnight at 4°C with primary antibody (beta-tubulin III 1:500; STEMCELL Technologies 60052) diluted in blocking buffer ...
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bioRxiv - Synthetic Biology 2021Quote: ... in borate buffer for primary culture cells or Poly-D-Lysine for cells from an iPSC background before being coated with either 10 µg/ml mouse laminin or 5 µg/ml human 521 Laminin (Stemcell Technologies Australia, Melbourne, Australia) respectively to facilitate cell adhesion.
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bioRxiv - Bioengineering 2022Quote: ... until a final volume of 200 ml and layered per 25 ml on top of 10 ml Lymphoprep™ (07851, StemCell technologies, Köln, Germany) in 50 ml centrifugal tubes ...
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bioRxiv - Neuroscience 2021Quote: ... and plated at 3 × 106 cells/well to allow the embryoid body (EB) formation in Neural Induction Media (NIM) + 10 μM Y-27632 (Stem Cell Technologies, Cat.No. 72308). They were allowed to attach for at least overnight ...
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bioRxiv - Immunology 2022Quote: ... until a final volume of 200 ml and layered per 25 ml on top of 10 ml Lymphoprep™ (07851, StemCell technologies, Köln, Germany) in 50 ml centrifugal tubes ...
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bioRxiv - Developmental Biology 2024Quote: ... and the organoid pellet was resuspended in 5-10 mL pre-cooled basal DMEM/F-12 medium containing 15 mM HEPES (Stem Cell Technologies, cat.# 36254) using a 10 mL pipette pre-wetted with 1% (vol/vol ...
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bioRxiv - Cancer Biology 2023Quote: ... Isolated T cells were seeded at a density of 1 x 106 cells/mL in RPMI medium supplemented with 10 ng/mL human recombinant interleukin 2 (IL2, Stemcell Technologies, Cat. No. 78145.2), 1µg/ml anti-human CD3 antibody (BioLegend ...
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bioRxiv - Cell Biology 2022Quote: ... the dissociated cells were resuspended in the transduction medium (1 uL to 5 uL of lentivirus, 4 to 8 ug/ml Polybrane[STR-1003-G, Sigma Aldrich], and 10 uM Y27632 [72304, StemCell Technologies] in mTeSR1), and centrifuged at 3200 xg for 30 to 90 min ...
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bioRxiv - Genomics 2022Quote: CD4 T-cells were isolated from 10-20 ml of peripheral blood using EasySep Direct Human CD4+ T cell Isolation kit (Stem Cell Technologies, cat #19662). The isolated CD4 T-cells were stored in Crystor CS10 (Stem Cell Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... were treated with drugs at a final concentration of 10 µM and mixed with MethoCult™ H4100 Base medium (Stem cell technologies, Canada, 04100) enriched with 20% FBS ...
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bioRxiv - Bioengineering 2023Quote: The hPSCs were cultured in NuwacellTM ncTarget hPSC Medium (Nuwacell Biotechnologies, RP01020, Hefei, China) with 10 μM Y-27632 (STEMCELL Technologies, 72304, BC, USA). The hNSCs were derived from hiPSCs which followed standard neurobiology protocols (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2020Quote: ... Fluorescence microscopy (Keyence Epi-Fluorescence microscope BZ-X710, Keyence, Japan) was performed using anti-dextran FITC antibody (Stemcell Technologies, Vancouver, Canada) co-incubated with T cells for 24 h in a chamber slide for live cell imaging ...
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bioRxiv - Cancer Biology 2020Quote: ... The supernatant was discarded and the cell pellet was re-suspended in an equivalent volume of HBSS and mixed with 25μL CTC enrichment antibody cocktail (RosetteSep™ CTC Enrichment Cocktail Containing Anti-CD36, STEMCELL Technologies) at room temperature for 20 min ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by incubation of the cells with a cocktail of lineage specific antibodies (CD5, CD11b, CD19, B220, Gr-1 and TER119, Stemcell Technologies) for negative selection of Lin-cells using an EasyStep immunomagnetic column (Stemcell Technologies) ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then incubated with a cocktail of lineage specific antibodies (CD2, CD3, CD11b, CD11c, CD14, CD16, CD19, CD24, CD56, CD61, CD66b, and GlyA; Stemcell Technologies) for negative selection of Lin− cells using an EasySep immunomagnetic column (Stemcell Technologies) ...
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bioRxiv - Bioengineering 2019Quote: ... and an “activated” population that was cultured in medium supplemented with 25 μl/ml tetrameric antibody against CD2/CD3/CD28 (StemCell Technologies). Quiescent and activated T cell populations were cultured separately for 48 hours at 37°C ...
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bioRxiv - Immunology 2021Quote: ... plasmablasts were enriched from the whole blood (day 7 after second vaccination) by negative selection using custom direct human plasmablasts isolation kit containing paramagnetic beads and antibodies for negative selection (StemCell Technologies). Enriched cells were stained 30 min on ice in a RoboSep buffer (StemCell Technologies ...
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bioRxiv - Immunology 2022Quote: Splenic macrophages were magnetically labeled and isolated using StemCell EasySep PE Positive Selection Kit II in concert with an F4/80 PE-conjugated antibody (StemCell Technologies). Briefly ...
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bioRxiv - Immunology 2024Quote: ... cells in a single-cell suspension were stained with fluorochrome-conjugated antibodies specific to surface antigens (listed in Supplemental Table 1) in Hanks’ Balanced Salt Solution (HBSS, STEMCELL Technologies) supplemented with 2% FBS ...
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bioRxiv - Immunology 2023Quote: ... the spleen of the mouse with the highest antibody titers was dissociated and fused with Sp2/0-Ag14 myeloma cells (ATCC) using the ClonaCell-HY Hybridoma Kit (StemCell Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... L0 and S7 cells were grown as floating spheres and maintained in NeuroCult NS-A Proliferation medium and 10% proliferation supplement (STEMCELL Technologies; Cat# 05750 and #05753), 1% penicillin–streptomycin (Thermofisher ...
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bioRxiv - Immunology 2021Quote: ... T cells from were then activated with Miltenyi MACS GMP T cell TransAct™ CD3/CD28 beads and seeded 1×10^6 T cells/ml in serum-free StemCell Immunocult™-XF media (StemCell Technologies, Vancouver, Canada) with clinical grade 20U/ml human IL-2 (Novartis)
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bioRxiv - Cancer Biology 2022Quote: ... L0 and S7 cells were grown as floating spheres and maintained in NeuroCult NS-A Proliferation medium and 10% proliferation supplement (STEMCELL Technologies; Cat# 05750 and #05753), 1% penicillin–streptomycin (Thermofisher ...
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bioRxiv - Cancer Biology 2024Quote: ... stem cells were cultured on Matrigel (FisherScientific, 354230) coated 6-well plates in mTeSR plus supplemented with 10% mTeSR plus Supplement (Stem Cell Technology, Cat 100-0276), and 1X Penicillin/Streptomycin or Primocin (FisherScientific NC9141851) ...
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bioRxiv - Cell Biology 2020Quote: ... A fraction of the cells was stained with an anti-human TRA-1-60 antibody conjugated with Alexa Fluor 488 (StemCell Technologies, Canada) and analysed by flow cytometry using a BD FACSCantoII according to manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... and for 1 h at 4 0C with a combination of conjugated antibodies to surface proteins TRA-1-60 (StemCell Technologies, 60064PE), TRA-1-81(StemCell Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... the sample was sorted using an APC-conjugated antibody to NOTCH1 (eBiosciences #17-5765-80) followed by APC magnetic selection using the EasySep kit (Stem Cell Tech) as per instructions and plated in PneumaCult medium with a SMAD inhibitor ...
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bioRxiv - Immunology 2020Quote: ... Human T cells were also isolated from the thymus tissue in humanized SRG rats via immunomagnetic selection using anti-human CD3 antibody (EasySep™ Human CD3 Positive Selection, Catalog # 17951, Stemcell Technologies) and treated without (vehicle ...
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bioRxiv - Immunology 2021Quote: ... induced tdTomato expressing thymocytes were labelled with biotinylated anti-CD4 and -CD8 antibodies and were enriched by immunoseparation of bulk thymocytes with Easysep Streptavidin Rapidspheres (Stem Cell Technologies) to reduce CD4+ and CD8+ cells ...