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Citations for Stemcell Technologies :
551 - 600 of 1538 citations for Methionine aminopeptidase 1 METAP1 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Neutrophils from HDs were purified by the EasySep Human Neutrophil Enrichment Kit (Stem Cell Technologies; > 99.7% purity) after centrifuging buffy coats onto Ficoll-Paque gradient density ...
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bioRxiv - Immunology 2020Quote: ... primary human NK cells were isolated by venipuncture followed by NK cell enrichment using RosetteSep (Stemcell Technologies) and separation by Ficoll-Paque density gradient ...
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bioRxiv - Microbiology 2021Quote: ... Human CD34+ HSC were isolated using a CD34+ HSC isolation kit (Stem Cell Technologies, Cambridge, MA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... H9 human ESCs were obtained from the WiCell Research Institute and cultured in mTeSR1 medium (STEMCELL Technologies). Embryoid bodies (EBs ...
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bioRxiv - Immunology 2019Quote: ... CD4+ T cells were isolated by negative selection (RosetteSep Human CD4+ T cell Enrichment Kit, Stemcell technologies) following the manufacturer’s procedure ...
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bioRxiv - Physiology 2019Quote: Monocytes were isolated from healthy apheresis donors by RosetteSep™ Human Monocyte Enrichment Cocktail (Stem Cell Technologies) by the University of Pennsylvania Human Immunology Core and provided as de-identified untraceable cells ...
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bioRxiv - Immunology 2020Quote: ... or αCD3/CD28 per manufacturer’s instructions (ImmuCult human CD3/CD28 T cell activator; StemCell Technologies, Cambridge, MA). After stimulation ...
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bioRxiv - Genomics 2020Quote: ... followed by immunomagnetic enrichment for the specific cell subset with EasySep Human cell enrichment kits (STEMCELL Technologies). B lymphocytes and CD4+ T lymphocytes were isolated from PBMCs by negative selection (STEMCELL Technologies ...
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bioRxiv - Immunology 2019Quote: One mM of EDTA and 50 µL/mL RosetteSep human monocyte enrichment cocktail (STEMCELL Technologies, Vancouver, Canada) were added to blood and incubated for 20 minutes at room temperature before dilution with an equal volume of PBS enriched with 2% FCS ...
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bioRxiv - Genomics 2021Quote: ... Frozen vials of primary human CD4+CD45RO+ memory T cells were purchased from STEMCELL Technologies (Cat #70031).
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bioRxiv - Immunology 2021Quote: ... from healthy buffy coat donors and incubated overnight with human recombinant Interleukin 15 (IL-15) (STEMCELL Technologies). NK cells were incubated with immune complexes ...
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bioRxiv - Cancer Biology 2020Quote: ... The NSC media was composed of Neurocult NS-A basal medium human (Stem Cell Technologies, Cat #05750) along with L-glutamine (Sigma ...
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bioRxiv - Cancer Biology 2020Quote: ... The NSC media was composed of Neurocult NS-A basal medium human (Stem Cell Technologies, Cat #05750) along with L-glutamine (Sigma ...
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bioRxiv - Microbiology 2019Quote: ... and CD4+ T cells were isolated using EasySep Human CD4+ T cell isolation kit (StemCell Technologies #17952). Cells were resuspended to 2.5 × 106 cells/mL in RPMI complete media supplemented with 10% FBS ...
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bioRxiv - Immunology 2020Quote: ... Plasma samples were diluted by half with PBS during RosetteSep human B cell processing (StemCell Technologies #15064), aliquoted ...
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bioRxiv - Immunology 2021Quote: Primary human CD3+ T cells were isolated from donor PBMCs via immunomagnetic negative selection (Stem Cell Tech). For thaw and culture ...
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bioRxiv - Immunology 2021Quote: ... NK cells were isolated from PBMCs using EasySep™ Human NK Cell Isolation Kit (Stemcell Technologies, BC). NK cells were co-cultured with K562-NL cells at different E:T ratios and treated with 10 μM of colistin for 5 hours at 37°C ...
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bioRxiv - Immunology 2021Quote: ... Primary CD8+ T cells were isolated using EasySep Human CD8+ T Cell Enrichment Kits (Stem Cell Technologies) and cultured in RPMI-1640 (ATCC ...
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bioRxiv - Immunology 2022Quote: ... Monocytes were purified from the PBMC population using the EasySep Human monocyte enrichment kit (STEMCELL Technologies; 19059), plated in 96-well plates and differentiated into microglia (iMDM ...
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bioRxiv - Immunology 2022Quote: ... coated with human type IV placental collagen in submerged PneumaCult-Ex Plus media (StemCell Technologies, Vancouver, Canada). When cells reached confluency ...
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bioRxiv - Neuroscience 2022Quote: ... human iPSCs were dissociated into single cells using Gentle Cell Dissociation Reagent (STEMCELL Technologies catalog # 100-0485) and were grown for 1 day with STEMdiff Neuronal induction medium (STEMCELL Technologies catalog # 05835) ...
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bioRxiv - Neuroscience 2022Quote: ... human iPSCs were routinely passaged as clumps onto Matrigel-coated plates with mTeSR+ media (StemCell Technologies #85850) according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... CD3+ T cells were isolated from PBMCs using EasySep™ Human T Cell Isolation Kit (StemCell Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... naïve CD4 T cells were purified using the naïve human CD4+ T cell isolation kit (StemCell Technology) and stimulated with immobilized anti-human CD3 (5 μg/ml ...
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bioRxiv - Immunology 2023Quote: Human PBMCs were isolated from buffy coats by density gradient centrifugation (Lymphoprep®, Stemcell Technologies Cat# 07811) and cultured overnight in T-cell medium (CTS OpTmizer® ...
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bioRxiv - Cancer Biology 2023Quote: ... T cells were negatively purified with EasySepTM beads (human T-cell isolation kit, STEMCELL Technologies, Vancouver, Canada). T cells were cultured in Advanced RPMI 1640 enriched with FBS (10%) ...
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Efficient Methods for Target Gene Manipulation in Haematopoietic Stem Cell Derived Human NeutrophilsbioRxiv - Cell Biology 2023Quote: ... medium was changed to SFEM II containing 10ng/mL recombinant human granulocyte colony stimulating factor (StemCell Technologies) and maintained in this medium until day 20 ...
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bioRxiv - Microbiology 2023Quote: ... CD4+ T cells were isolated using the EasySep Human CD4+ T cell Isolation Kit (Stemcell Technologies, 17952) and subsequently activated using the T Cell Activation/Expansion Kit (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2023Quote: Human iPSCs were differentiated for 12 days using the STEMdiff™ Hematopoietic Kit (#05310, STEMCELL Technologies, Inc.) [43] ...
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bioRxiv - Immunology 2023Quote: Neutrophils of healthy blood donors were isolated using EasySep™ Direct Human Neutrophil Isolation Kit (Stemcell Technologies). Neutrophils were stimulated with immune-complexes as described above and supernatants collected after 4 h ...
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bioRxiv - Immunology 2023Quote: ... B cells were isolated using an EasySep Human Pan-B Cell Enrichment kit (Stemcell Technologies, Vancouver, Canada).
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bioRxiv - Cancer Biology 2023Quote: ... The human MLL-AF9-transduced cells were cultured in StemSpan SFEM II medium (#ST-09655, STEMCELL Technologies) containing 1% penicillin– streptomycin together with 10 ng/ml rhSCF (#255-SC ...
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bioRxiv - Immunology 2023Quote: ... Cells were enriched for CD8+ population using the EasySep Human CD8+ T Cell Isolation Kit (Stemcell Technologies) and CD8+ cells were used for analysis.
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bioRxiv - Genomics 2023Quote: Primary CD25-CD4+ effector T cells were isolated from fresh Human Peripheral Blood Leukopaks (STEMCELL Technologies, #70500) from healthy donors ...
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bioRxiv - Immunology 2023Quote: ... and monocytes were isolated from PBMCs by EasySep Human T Cell Isolation Kit (StemCell Technologies, Vancouver, Canada), MACSxpress Whole Blood NK Cell Isolation Kit (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2023Quote: H9 human embryonic stem cells (hESCs; female, WA09, WiCell) were cultured in mTeSR Plus medium (STEMCELL Technologies) with 1% Penicillin-Streptomycin in 8 mg/mL Matrigel-coated (Corning ...
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bioRxiv - Synthetic Biology 2023Quote: ... and ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator (25 µL/mL) (Stemcell Technologies, Cat.#10990) and cultured for 1.5 days at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2023Quote: ... and T cells were purified using the EasySep™ Human T Cell Isolation Kit (StemCell Technologies, 17951). Once purified ...
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bioRxiv - Cell Biology 2024Quote: ... Epithelial cells were enriched using the EasySep™ Human EpCAM Positive Selection Kit II (Stemcell Technologies, #17846) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Naïve CD4+ T cells were isolated using EasySep Human Naïve CD4+ T Cell Isolation Kit (Stemcell technologies), according to manufacturer’s indications.
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bioRxiv - Bioengineering 2024Quote: ... T cells were isolated using the EasySepTM Human T cell isolation kit (StemCell Tech., Saint-Egrève, France) and cultured in complete X-Vivo15 medium ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were then treated with 20 uL/mL of Immunocult Human Treg Differentiation Supplement (StemCell Technologies) containing recombinant human TGF-B1 and all-trans retinoic acid ...
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bioRxiv - Immunology 2021Quote: ... BMPCs were enriched from bone marrow mononuclear cells using EasySep Human CD138 Positive Selection Kit II (StemCell Technologies) and immediately used for ELISpot or cryopreserved in 10% dimethyl sulfoxide in FBS.
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bioRxiv - Immunology 2021Quote: ... B cells were enriched from PBMC by first using EasySep Human Pan-B cell Enrichment Kit (StemCell Technologies), and then stained with CD20-PB (2H7 ...
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bioRxiv - Immunology 2021Quote: ... NK cells were isolated from whole blood with the EasySepTM Direct Human NK Cell Isolation Kit (StemCell Technologies). The gating strategy for isolation of CD56+CD3- NK cells is shown in Figure S1 (Figure 2 – Supplement 1) ...
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bioRxiv - Immunology 2022Quote: ... CD14+ CD16- monocytes were isolated using magnetic negative selection (EasySep Human Monocyte Isolation Kit, StemCell Technologies, Cambridge, Massachusetts) and differentiated into macrophages by culture in RPMI 1640 (Lonza ...
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bioRxiv - Cancer Biology 2020Quote: Frozen human bone marrow mononuclear cells were thawed and washed twice with Iscove’s Modified Dulbecco’s Medium (StemCell Technologies) containing 10% fetal bovine serum (FBS) ...
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bioRxiv - Immunology 2020Quote: ... Monocytes were isolated from PBMC by negative selection using the EasySep™ Human Monocyte Isolation Kit (STEMCELL Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: T and B cell lineage depletion was performed using RosetteSep Human Hematopoietic Progenitor Cell Enrichment Cocktail (Stemcell Technologies) and Ficoll-Paque density gradient centrifugation from routine red cell exchange apheresis performed at Texas Children’s Hospital or Columbia University New York Presbyterian Hospital ...
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bioRxiv - Genetics 2021Quote: ... 100ng/ml human interleukin 6 (hIL-6) (all obtained from EuroBiosciences) and 35nM UM171 molecule (Stem Cell Technologies). Cells were cultured under normoxic conditions ...