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Citations for Stemcell Technologies :
551 - 600 of 679 citations for Anion Exchange Membranes Thickness 10 13 µm since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... the culture medium was supplemented with 1 mM EDTA and 10 μM Y-27632 (StemCell Technologies, Vancouver, Canada), and cells were allowed to incubate for 24 hours ...
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bioRxiv - Developmental Biology 2024Quote: ... and replated in MEF-CM or mTeSR™ containing 10 μM ROCK inhibitor Y-27632 (STEMCELL Technologies, 72308). G-banding karyotyping was performed at Cell Line Genetics to verify chromosomal status prior to initiating experiments.
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Peripheral neuropathy linked mRNA export factor GANP reshapes gene regulation in human motor neuronsbioRxiv - Neuroscience 2021Quote: ... The cells were cultured in feeder-free conditions in 10 cm culture dishes in TeSR-E8 (Stem Cell Tech) medium on SyntheMAX (2µg/cm2 ...
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bioRxiv - Bioengineering 2020Quote: ... T cells were frozen at 10–20 × 106 cells per 1 mL of Cryostor CS10 (STEMCELL Technologies, Cambridge, MA) and thawed into culture for knockout experiments.
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bioRxiv - Cell Biology 2020Quote: Mammary glands from 10-16 weeks old C57BL/6J females were minced and dissociated using collagenase/hyaluronidase (STEMCELL Technologies) diluted (1:10 ...
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bioRxiv - Cell Biology 2022Quote: ... they were cultured during 24 hours in the presence of 10 μM of Rock inhibitor Y-27632 (StemCell Technologies).
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bioRxiv - Cell Biology 2024Quote: ... Cells were initially plated on 10% Matrigel coated plates and expanded in PneumaCult™-Ex Plus Medium (Stemcell Technologies) + 1x PneumaCult™-Ex Plus Supplement (Stemcell Technologies ...
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bioRxiv - Developmental Biology 2023Quote: ... Aggregates were cultured from day 8 to day 10 in A-RPMI or TeSR™-E6 (StemCell Technologies 05946) medium supplemented with 25% M.CM ...
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bioRxiv - Neuroscience 2023Quote: ... the hiNPCs were singularized with Accutase for 10 min at 37 °C and 5 % CO2 (#07920, Stemcell Technologies, Canada) and centrifuged (300 g ...
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bioRxiv - Immunology 2023Quote: ... The diluted collar blood was then layered on top of 10 mL Lymphoprep™ density gradient medium (Stemcell Technologies). After centrifugation at 1200 g for 20 minutes ...
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bioRxiv - Bioengineering 2023Quote: ... 1G4 PBMCs loaded onto pMHC nanovials were sorted based on calcein AM and NGFR signal and reconstituted with 0.75 mL media and 0.25 mL of 10 mg/mL Collagenase Type II solution (STEMCELL Technologies), followed by a 2 hour incubation at 37°C ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% heat-inactivated FCS and stimulated with Immunocult™ Human CD3/CD28 T cell Activator (Stemcell Technologies) (25 µL/ 106 cells ...
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bioRxiv - Microbiology 2023Quote: In vitro IL-10 assays were also performed using human Peripheral Blood Mononuclear Cells (PBMCs) (STEMCELL Technologies, Cat. # 70025.1). The assay was performed as described above ...
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bioRxiv - Immunology 2024Quote: ... Splenocytes were then fused to Sp2/10-Ag14 cells with polyethylene glycol using the ClonaCell Hybridoma kit (StemCell Technologies). Fused cells were cultured overnight in T-75 flasks to recover ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The resulting cell mass was resuspended in mTeSR1 medium supplemented with 10 μM Y-27632 (StemCell Technologies, Cat# 72307) and the single-cell suspension was seeded on Matrigel-coated 24-well plates at a ratio of ∼1.25 × 105 cells/cm2 ...
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bioRxiv - Cancer Biology 2024Quote: ... Total PBMCs were then activated (10 mio PBMC/ml) with CD2/CD3/CD28 Immunocult T-cell activator (Stemcell Technologies) in Prime XV chemically-defined T-cell medium (IrvineScientific) ...
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bioRxiv - Cancer Biology 2020Quote: ... Skin was disinfected with 10% iodine in phosphate saline buffer (PBS) and incubated with 2U/ml of dispase (STEMCELL technologies) overnight at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... MDS-L cell line was cultured in the presence of 10 ng/mL human recombinant IL-3 (Stem Cell Technologies). The MDS/AML patient samples used in this study were obtained with written informed consent under approval by the IRBs of the Albert Einstein College of Medicine ...
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bioRxiv - Genomics 2021Quote: ... For crypt isolation the intestinal tissue pieces were transferred into 10 ml of Gentle Cell Dissociation Reagent (07174, STEMCELL Technologies) and incubated for 15 mins at room temperature (RT) ...
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bioRxiv - Cancer Biology 2021Quote: ... recombinant mouse Interleukin-3 (6 ng/ml) and recombinant human Interleukin-6 (10 ng/ml) (all from Stem Cell Technologies). 200ul fresh SFEM medium with cytokines was added on Day 5 and 8 and cells were counted on Day 5 and 10.
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bioRxiv - Cell Biology 2024Quote: FACS-sorted GFPhi MAPs from mammary glands of PdgfrαH2B-eGFP mice were seeded onto collagen-coated wells of a 24-well plate at 20,000 cells per well and cultured in DMEM media with 10% FBS and 10ng/ml bFGF (STEMCELL Technologies). Following 5 days of culture ...
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bioRxiv - Molecular Biology 2024Quote: ... and differentiation protocol as reported.10 At day -1 iPSC were prepared in mTeSR™1 (StemCell Technologies Cambridge, MA) and plated at approximately 40 colonies per well on Matrigel pre-coated wells ...
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bioRxiv - Developmental Biology 2024Quote: ... for 10 min at 37° C and seeded on to Geltrex-coated 12-well plates at a density of 700,000 cells in neural induction medium + 10 uM Y-27632 (Stemcell Technologies) and cultured for 7 days with daily medium exchange ...
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A scalable, GMP-compatible, autologous organotypic cell therapy for Dystrophic Epidermolysis BullosabioRxiv - Bioengineering 2023Quote: ... counted and added at 1.2 million cells per microwell in AggreWell media containing 10 μM ROCK inhibitor (StemCell technologies 72302). Media was carefully changed the following day to remove ROCK inhibition ...
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bioRxiv - Cell Biology 2022Quote: ... GINS precursors were dissociated by 5-10 min TrypLE treatment and aggregated (typically 2.0-2.4 million cells/well) in AggreWell400 (STEMCELL Technologies, 34450) using the manufacturer’s recommended protocol ...
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bioRxiv - Cell Biology 2024Quote: F814 and WT bone marrow cells were cultured for 10 days in MesencultTM Expansion Kit for mouse (Stemcell tech, 05513) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... supplemented with required additional growth factors (20 ng/mL human recombinant EGF, 10 ng/mL human recombinant bFGF, 2μg/mL heparin, STEMCELL Technologies). Cultures were seeded on ultra-low attachment culture ware (Corning CLS3471 ...
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bioRxiv - Cell Biology 2020Quote: ... Cell suspension-matrigel mixture was placed in a transwell and incubated in growth media with 10 μM ROCK inhibitor (Cat#72252; STEMCELL Technologies) in a 24 well plate for 48 hours ...
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bioRxiv - Neuroscience 2021Quote: ... Suspension was mixed with 250 ul of 50% Iodixanol solution (25 mM KCl, 5 mM MgCl2, 10 mM Tris pH 8.0, 50% Iodixanol (60% stock from STEMCELL Technologies, 7820), 1x protease inhibitor (Roche ...
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bioRxiv - Cell Biology 2022Quote: ... and resuspended to a final concentration of 10 000 cells/50 μL in EB media: mTeSR™ Plus medium (StemCell Technologies), 50 ng/mL BMP-4 (R&D ...
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bioRxiv - Immunology 2021Quote: ... PBMC and plasma samples were isolated after whole blood centrifugation 1200 g for 10 minutes at room temperature in SepMate tubes (STEMCELL Technologies). Plasma was stored at −80°C until analysis ...
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bioRxiv - Physiology 2020Quote: ... Single-cell suspensions were plated at a density of 1.2 × 105 cells/cm2 and cultured overnight in mTeSR medium supplemented with 10 μM Y-27632 dihydrochloride (Stem Cell Technologies). Once the monolayer reached approximately 80% confluence (usually the following day) ...
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Parallelized engineering of mutational models using piggyBac transposon delivery of CRISPR librariesbioRxiv - Bioengineering 2020Quote: ... setting the instrument to deposit one cell into each well in a 96-well plate containing working medium with 10% CloneR cloning supplement (Stemcell Technologies). Filled plates were transferred to the 37°C incubator and left undisturbed for 48 hours after sorting ...
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bioRxiv - Bioengineering 2021Quote: ... 50 ng/mL recombinant human FMS-like tyrosine kinase 3 ligand (Flt-3) and 10 ng/mL recombinant human thrombopoietin (TPO; StemCell Technologies). On the following day ...
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bioRxiv - Neuroscience 2021Quote: ... were isolated from E13.5 pregnant CD-1 mice and cultured on poly-D-lysine (20 μg/mL) and laminin (10 μg/mL)-coated plates in NeuroCult Basal Medium containing NeuroCult Proliferation Supplement (StemCell Technologies) and 20 ng/mL EGF ...
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bioRxiv - Neuroscience 2021Quote: ... The media was changed daily and the iPSC cultures were split into 1:6-1:10 every 5 days using ReLeSR (Stemcell Technologies).
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bioRxiv - Cell Biology 2021Quote: ... The resulting single cell suspension was than washed with 10 mL of cold RPMI and resuspended in 1 mL of Robosep buffer (STEMCELL technologies) prior to the negative selection of B- or T-cells ...
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bioRxiv - Developmental Biology 2022Quote: ... single hESCs were seeded into a spinner flask at a density of 1e6 cells/mL in mTeSR1 media containing 10 μM Rock inhibitor Y-27632 (STEMCELL Technologies) to allow formation of clusters ...
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bioRxiv - Cancer Biology 2022Quote: ... the cells where count and seeded at a density of 2×10^4 cells per replicate into cytokine-supplemented methylcellulose medium (MethoCult M3434, Stemcell Technologies). After 10-14 days the colonies propagated and scored ...
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bioRxiv - Neuroscience 2022Quote: ... Harvested spheroids then were transferred into 10-cm ultra-low adhesion plates in Essential 6 medium containing dual SMAD pathway inhibitors [100 nM LDN193189 (STEMCELL Technologies) and 10 μM SB431542] and 2.5 μM XAV939 (Cayman) ...
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bioRxiv - Cell Biology 2022Quote: ... and cultured in new 6-well plate using mTeSR™ plus medium supplemented with 10 μM Y-27,632 Rock inhibitor (StemCell Technologies). Pluripotency of all lines was established by analyzing the expressions of canonical stemness genes (SOX2 ...
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bioRxiv - Developmental Biology 2022Quote: ... For HLC differentiation cells were seeded onto Matrigel™ coated 24-well tissue culture plates at 130,000 cells/ cm2 in mTeSR™ Plus containing 10 μM ROCK inhibitor (STEMCELL Technologies). After 24 hours the differentiation protocol was commenced using STEMdiff™ Definitive Endoderm Kit (STEMCELL Technologies ...
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bioRxiv - Immunology 2021Quote: ... before centrifugation at 300 xg for 10 minutes followed by resuspension in 100 μl of CryoStor-CS10 freezing media (StemCell Technologies). The UVC preparations for use in non-human primate studies were analyzed for endotoxin levels (Wickham Laboratories Ltd ...
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bioRxiv - Developmental Biology 2021Quote: ... approximately half of the neuruloids on each coverslip were scraped off and treated for 10 min at 37 °C with Accutase (Stemcell Technologies). The remaining neuruloids were fixed for immunofluorescence analysis as a quality control ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were re-plated in mTeSR1 medium supplemented with 1% penicillin-streptomycin (Thermo Fischer Scientific) and 10 mM Rho-associated protein kinase (ROCK) inhibitor (Stemolecule Y-27632, STEMCELL Technologies) for 24 hr ...
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bioRxiv - Immunology 2021Quote: ... PBMCs were enriched from whole blood (day 10 after first, and day 7 after second vaccination) using direct PBMCs isolation kit (StemCell Technologies). For singe-cell antibody secretion and paired antibody sequencing studies ...
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bioRxiv - Bioengineering 2020Quote: ... MSPCs were isolated from the crushed bone following a commercially available protocol and cultured for 10 days prior to collection and use (#05513, Stemcell Technologies).
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bioRxiv - Cancer Biology 2021Quote: Tumor tissue excised from the brain was mechanically dissociated using a 70 um cell strainer in phosphate-buffered saline (PBS) with 10% FBS and 0.1 mg/mL DNAse I (Stem Cell Technologies). Cells were then resuspended in 25% isotonic Percoll solution and centrifuged for 20 minutes at 18°C with acceleration and deceleration set to minimum ...
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bioRxiv - Immunology 2021Quote: ... PBMC and plasma samples were isolated after whole blood centrifugation 1200 g for 10 minutes at room temperature in SepMate tubes (STEMCELL Technologies). Plasma was stored at -800C until analysis ...
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bioRxiv - Neuroscience 2022Quote: ... Medium was replaced every other day and cells were passaged ~1:10 onto fresh Matrigel-coated vessels using ReLeSR (StemCell Technologies) when 90% confluent.