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Citations for Stemcell Technologies :
501 - 550 of 1118 citations for Siglec 15 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... we used mouse-specific and human-specific T cell negative isolation MACS (STEMCELL Technologies, Canada). We plated 0.5–1 × 106 T cells per well in 96-well V-bottom tissue culture plates ...
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bioRxiv - Immunology 2023Quote: ... CD14+ Mo were FACS sorted from frozen human bone marrow mononuclear cells (Stem Cell Technologies) on a BD FACS Melody Cell Sorted (BD Biosciences ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... PBMCs were activated with ImmunoCult human CD3/CD28 T cell activator (STEMCELL Technologies; Cat. # 10971) and 50 U/mL IL-2 (BD-Pharmingen ...
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bioRxiv - Cancer Biology 2023Quote: T cells were enriched using the Easy Sep Human T cell Isolation Kit (Stemcell Technologies) according to the manufacturer’s instructions ...
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Human CCR6+ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticitybioRxiv - Immunology 2023Quote: ... Stimulation was done using ImmunoCult human CD3/CD28/CD2 T Cell Activator (Stem Cell Technologies) in non-polarizing conditions using anti-IL-4 (5 μg/ml) ...
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bioRxiv - Genomics 2023Quote: ... WTC11 human iPSCs (RRID:CVCL_Y803) were cultured in mTeSR plus medium (Stemcell technologies, Catalog # 100-0276) and passaged using ReLeSR (Stemcell technologies ...
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bioRxiv - Immunology 2023Quote: ... and CD34+ HSPCs were enriched using the EasySep Human CD34+ Positive Selection kit (Stemcell Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... medium was replaced by 250µL of Intesticult ODM human basal medium (StemCell technologies, 100-0214), allowing generation of intestinal organoid cultures with physiological representations of the stem cell and differentiated population ...
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bioRxiv - Developmental Biology 2023Quote: Shef6 human ESCs were cultured on Matrigel-coated plates in mTESR medium (05825, STEMCELL Technologies) at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... EasySep™ human CD34 positive selection kit II was procured from Stem cell technologies (17856). Ficoll® Paque PLUS density gradient media was purchased from Sigma Aldrich (GE17-1440-03).
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bioRxiv - Neuroscience 2023Quote: ... Human DS and control iPSCs were cultured and maintained in mTeSR1 medium (Stem Cell Technologies) in tissue culture dishes coated with hESC-qualified Matrigel (Corning) ...
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bioRxiv - Cell Biology 2023Quote: ... H1 human embryonic stem-cell (H1-hESC) line was cultured in mTeSR1 medium (STEMCELL Technologies) under 5% CO2 at 37℃ and was sub-cultured when reaching 80% confluence ...
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bioRxiv - Bioengineering 2023Quote: ... Human induced pluripotent stem cell (hPSC) line PGP1 was cultured with mTeSR1 (STEMCELL Technologies, #85850) in tissue culture 6-well plates coated with reduced growth factor Geltrex (Gibco ...
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bioRxiv - Microbiology 2023Quote: A three-stage protocol was used to differentiate human bone marrow CD34+ cells (STEMCELL Technologies) down the erythroid lineage as previously described.37 Briefly ...
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bioRxiv - Immunology 2023Quote: ... PBMCs from human blood samples were isolated on a density gradient medium (Lymphoprep, STEMCELL Technologies). Cells were incubated 4°C for 10 min with anti-mouse or anti-human CD16/CD32 (BD Pharmingen ...
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bioRxiv - Cancer Biology 2024Quote: ... The normal human CD34+ HSPCs used in all functional assays were purchased (Stem Cell Technologies). Cells were cultured in Iscove’s modified Dulbecco’s medium with 10% FBS ...
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bioRxiv - Microbiology 2024Quote: ... isolated from fresh blood samples using a RosetteSep Human NK Cell Enrichment Cocktail (StemCell Technologies), were infused into each lane ...
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β-1,6-glucan plays a central role in the structure and remodeling of the bilaminate fungal cell wallbioRxiv - Microbiology 2024Quote: Neutrophils from healthy donors were isolated with EasySepTM Direct Human Neutrophil Isolation Kit (STEMCELL Technologies) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: Day 15 H9 hESC-derived enteric crestospheres were dissociated into single cells (accutase, Stemcell Technologies, 07920, 30 min, 37 °C), resuspended in ENC medium and were transferred into 384-well plates ...
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bioRxiv - Microbiology 2021Quote: ... Samples were diluted with an equal volume of PBS and then overlaid on 4.5 ml of Ficoll in a 15 ml SepMateTM tube (STEMCELL Technologies) and spun at 300 ×g for 10 min at 20 °C to separate plasma and cells ...
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bioRxiv - Bioengineering 2021Quote: ... The microcapsules carrying HUES-8 cells were collected into 15 mL tube filled with 5 mL E8 medium (STEMCELL Technologies), then distributed into a 6 well-plate at density of 2000 capsules/well and incubated at 37 □ with 5% CO2 ...
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bioRxiv - Bioengineering 2021Quote: ... the cuvettes were allowed to rest for 15 minutes before the T cells were gently moved to antibiotic-free medium containing 1µg/mL DNase (StemCell Tech) at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... The rest of the whole blood layer was diluted with an equal volume of PBS and then overlaid on 4.5 ml of Ficoll in a 15 ml SepMateTM tube (STEMCELL Technologies) and spun at 1200 RPM for 10 min at 20 °C to separate peripheral blood mononuclear cells (PBMCs) ...
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bioRxiv - Cell Biology 2019Quote: ... mice were injected with 5-15×105 NK cells purified using the NK cell enrichment negative selection kit (STEMCELL Technologies).
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bioRxiv - Microbiology 2022Quote: ... The rest of the whole blood layer was diluted with an equal volume of PBS and then overlaid on 4.5 ml of Ficoll in a 15 ml SepMateTM tube (STEMCELL Technologies) and spun at 1200 rpm for 10 min at 20 °C to separate peripheral blood mononuclear cells (PBMCs) ...
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bioRxiv - Cell Biology 2022Quote: ... The diluted blood was layered on top of 15 mL Lymphoprep™ density gradient medium (StemCell Technologies Inc., Vancouver, Canada) in a 50 mL Falcon tube ...
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bioRxiv - Immunology 2023Quote: ... Diluted blood was overlaid carefully on SepMate-15 tubes each containing up to 4 mL Lymphoprep (STEMCELL Technologies, catalog #07851). The layered blood was centrifuged at 1,200 × g for 10 minutes at RT ...
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bioRxiv - Developmental Biology 2023Quote: ... and supplied with Basal culture medium consisting of DMEM/F12 (with 15 mM HEPES) (Catalog no. 36254, Stem Cell Technologies), 1x GlutaMAX (Catalog no ...
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bioRxiv - Molecular Biology 2021Quote: ... the pooled samples were diluted 1:1 with PBS+2% FBS and loaded into SepMate-15™ tubes with 4.5 mL of Lymphoprep™ with a density of 1.077g/mL (StemCell Technologies). Cells were centrifuged at 1200x g for 20min at 4°C ...
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bioRxiv - Immunology 2019Quote: ... Cells were then permeabilized with 0.3% Triton-X for 15 min followed by 30 min block in 0.1% Triton-X + 5% normal rat serum (Stem Cell Technologies). Cells were incubated in Drp1 p616 Ab overnight at 4°C in 0.1% Triton-X + 1% BSA and then in secondary antibodies (AF488 Goat anti-Rabbit) ...
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bioRxiv - Immunology 2024Quote: Female CatchupIVM-red and FVB/N mice aged 8-15 weeks were treated with 5 µg mouse recombinant G-CSF (STEMCELL Technologies) by intraperitoneal injection (i.p. ...
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bioRxiv - Developmental Biology 2023Quote: ... NMP-MSCs were suspended as a sphere in a 15-ml conical tube and cultured in MesenCult-ACF Chondrogenic Differentiation medium (STEMCELL Technologies) for 4 weeks.
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bioRxiv - Immunology 2023Quote: Different blood compartments (plasma, PBMCs, and RBCs) were separated by density gradient centrifugation using SepMate-15 tubes (STEMCELL Technologies, catalog #85415) to reduce blood processing time and user variability ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were kept in MEF medium for 48 h and then the medium was changed to mouse embryonic stem cell (mESC) medium: DMEM with the addition of 15% mouse ESC maintenance FBS (STEMCELL Technologies), 100 units ml−1 ESGRO mouse leukaemia inhibitory factor (mLIF ...
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bioRxiv - Developmental Biology 2021Quote: Human ESCs were differentiated into neural progenitor cells (NPCs) using the STEMdiff neural system (STEMCELL Technologies). Briefly ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 2% FBS and 1.5ug/mL anti-human CD32 antibody (Clone IV.3; STEMCELL Technologies). Cells were stained for 20-30 minutes on ice with 1:200 anti-CD11b-BV711 (Clone M1/70 ...
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bioRxiv - Microbiology 2019Quote: ... or the EasySep Human CD4+ T Cell Isolation Kit (STEMCELL Technologies, Vancouver, BC, Canada, Cat. #17952) according to manufacturer’s instructions ...
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Optimization of AAV6 transduction enhances site-specific genome editing of primary human lymphocytesbioRxiv - Molecular Biology 2021Quote: ... and magnetic bead selection using an EasySep™ Human CD34 Positive Selection Kit (STEMCELL Technologies Inc.). The resulting CD34+ HSPCs were cultured in HSPC media ...
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Understanding immune-driven brain aging by human brain organoid microphysiological analysis platformbioRxiv - Bioengineering 2022Quote: The human embryonic stem cell line (WA01, WiCell) was maintained with mTeSR plus medium (Stemcell Technologies) on 6 well plates coated with Matrigel (Corning) ...
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bioRxiv - Immunology 2021Quote: ... CD34+ precursors cells were isolated from PBMCs (EasySep™ Human CD34 Positive Selection Kit, STEMCELL Technologies). CD34+ cells were grown under serum-free conditions using StemSpan™ medium (STEMCELL Technologies ...
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bioRxiv - Immunology 2019Quote: ... Pure cell populations were isolated using EasySep Human negative selection kits (STEM CELL technologies, >95% purity). In addition ...
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bioRxiv - Systems Biology 2021Quote: ... Peripheral Blood Mononuclear Cells (PBMCs) were isolated using the Direct Human PBMC Isolation Kit (StemCell Technologies) according to the manufacturer’s protocol and washed/resuspended in custom RPMI-1640 deficient in biotin ...
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bioRxiv - Cancer Biology 2020Quote: ... Red blood cell lysis and leukocyte depletion (EasySep Human CD45 Depletion Kit II #17898, STEMCELL Technologies) of MPE were performed before processing with 10X Genomics ...
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bioRxiv - Cancer Biology 2020Quote: ... Mesenchymal: Adipocyte differentiation was carried out using the MesenCult Human Adipogenic Differentiation kit (STEMCELL Technologies #05412) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: Primary human T cells were isolated by negative selection for CD3+ populations (STEMCELL technologies, Easy Sep) from anonymous healthy human donor blood collected by leukopheresis and purchased from STEMCELL technologies ...
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bioRxiv - Immunology 2021Quote: ... Human CD8+ T cells were cultured in ImmunoCult cell culture media (Stemcell Technologies, Cat no. 10981) supplemented with 35 ng/ml human IL-2 (Peprotech ...
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bioRxiv - Immunology 2021Quote: ... plasma cells were isolated using the EasySep Human CD138 Positive Selection Kit II (Stemcell Technologies, #17877) for analysis in a companion study (manuscript in preparation) ...
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bioRxiv - Neuroscience 2022Quote: Cerebral organoids were differentiated from human iPSCs and cultured with STEMdiff Cerebral Organoid Kit (STEMCELL Technologies) according to the manufacture’s protocol ...
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bioRxiv - Immunology 2022Quote: ... Neutrophils were isolated with the EasySep Direct Human Neutrophil Isolation Kit (STEMCELL Technologies; cat. no. 19666).
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bioRxiv - Genetics 2022Quote: ... Human KOLF_2 (HIPSCI, www.hipsci.org) were grown in feeder-free conditions in TeSR-E8 medium (StemCell Technologies) on Synthemax (Corning ...