Labshake search
Citations for Stemcell Technologies :
501 - 550 of 551 citations for Recombinant Mouse PPAR gamma Protein His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2021Quote: ... enriched lineage negative cells were further purified to obtain an enriched Lin-Sca-1+ c-kit+ (LSK) population using EasySep™ Mouse Sca1 and cKit Positive Selection kits (#18756 & #18757, Stemcell Technologies, CA). For ROS studies ...
-
bioRxiv - Immunology 2021Quote: CD8+ T cells were isolated from spleens of WT C57BL/6 mice by negative selection using the EasySep™ Mouse CD8+ T cell isolation kit (Stem Cell Technologies) according to the manufacturer’s protocol and cultured in complete media ...
-
bioRxiv - Immunology 2020Quote: CD4+ T cells were isolated from spleen and peripheral lymph nodes from indicated mice by negative selection using the EasySep™ Mouse CD4+ T Cell Isolation Kit (StemCell Technologies Inc). 1.75 x 105 cells were cultured for 4 days in RPMI1640 supplemented with 10% heat-inactivated FCS ...
-
bioRxiv - Developmental Biology 2021Quote: NS cell cultures were established from E13.5 mouse ventral telencephalon as previously described (Conti et al. 2005) and maintained in mouse Neurocult basal media supplemented with proliferation supplement (Stem Cell Technologies, #05702,), 1% Penicillin-Streptomycin (ThermoFisher ...
-
bioRxiv - Microbiology 2020Quote: DCs were isolated from SPLNs of mice at 7 weeks of age using a mouse CD11c positive selection kit II (STEMCELL Technologies, Vancouver, BC). Fecal lysates used for stimulation were prepared by resuspending fecal samples (100 mg feces in 1ml of PBS ...
-
bioRxiv - Immunology 2021Quote: Single cell suspensions were prepared from multiple OT-I and OT-I;IL-7Rα449F mice spleens and CD8 T cells were purified using the CD8 T cell negative selection kit (EasySep™ Mouse CD8+ T Cell Isolation Kit) from Stem Cell Technologies. 1×106 cells were transferred I.V ...
-
bioRxiv - Immunology 2020Quote: ... CD4+ T-cells were isolated from the pooled cell suspensions using an EasySep(tm) Mouse CD4+ T Cell Isolation Kit from Stemcell Technologies (Vancouver, Canada) following the manufacturer’s instructions ...
-
bioRxiv - Immunology 2022Quote: ... T cells were isolated from tumor-free wild type B6 mice using the mouse CD8 T cell isolation kit (STEMCELL Technologies cat #19853), labeled with Cell Trace Violet (Thermo Fisher cat #C34557 ...
-
bioRxiv - Cell Biology 2024Quote: ... and iliac crests of each mouse. HSPCs were isolated using CD117 MicroBeads (Miltenyi cat. #130-091-224) and cultured in StemSpan SFEM II (StemCell Technologies cat. #09605) with 10 ng/mL recombinant murine SCF (STEMCELL Technologies ...
-
bioRxiv - Immunology 2023Quote: ... Tcon and Treg cells were isolated from the spleen by pre-enrichment with EasySep Mouse CD4+ T cell Isolation Kit (STEMCELL Technologies, Cat# 19852), and FACS sorting ...
-
bioRxiv - Cancer Biology 2023Quote: Splenic B cells were isolated from 12-month-old female and male mice (n=3-4/group) after 7 weeks of BBN exposure using EasySep Mouse Pan B cell negative isolation kit (Cat# 19844, StemCell technologies, BC, Canada). The purity and viability of enriched B cells was >90% as assessed by anti-B220 and anti-CD19 mAb staining using flow cytometry (Fig ...
-
bioRxiv - Developmental Biology 2023Quote: ... 2019) were cultured on mitotically inactivated mouse embryonic fibroblasts (MEFs; DR4 ATCC) in PXGL medium47 or in RSeT medium (Stem Cell Technologies 05969), at 37°C ...
-
bioRxiv - Immunology 2023Quote: ... Cells were filtered through a 70 μm nylon strainer and further purified with the EasySep mouse neutrophil enrichment kit (StemCell Technologies, Vancouver, Canada), following the manufacturer’s instructions.
-
bioRxiv - Immunology 2023Quote: ... by following the Protocol described in the Technical Bulletin: Intestinal Epithelial Organoid Culture with IntestiCult Organoid Growth Medium (mouse) (StemCell Technologies, Document #28223). Organoids were maintained at 37°C and 5% CO2 as 3D spheroids in Matrigel (Corning ...
-
bioRxiv - Immunology 2023Quote: Naïve CD4+ T cells were isolated from single-cell suspensions from spleen tissue by negative selection using the EasySep Mouse CD4+ T cell Isolation Kit (# 19852, StemCell Technologies, Cambridge, MA) and differentiated into different T helper (Th ...
-
bioRxiv - Immunology 2024Quote: ... The lungs were collected for single cell suspension and ILC enrichment (by Mouse Pan-ILC Enrichment Kit; STEMCELL Technologies, Vancouver, British Columbia, Canada). The dose of Alt was 30 µg/mouse ...
-
bioRxiv - Immunology 2024Quote: ... splenic murine t cells were isolated from C57BL/6 mice using EasySep™ Mouse T Cell Isolation Kit (STEMCELL Technologies; Cat. No. 19851). Glioma cells were labeled with Cr51 (0.05 mCi ...
-
bioRxiv - Immunology 2024Quote: ... CD8+ T cells were purified from the spleen of age- and sex-matched OT-I mice using the EasySep Mouse CD8+ T Cell Isolation Kit (STEMCELL Technologies, Cat. #19853) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2024Quote: ... and spleen cells were analyzed by flow cytometry after splenocytes were enriched for donor-derived cells by using EasySep™ Mouse Streptavidin RapidSpheres™ Isolation Kit (Stemcell Technologies) and biotin-conjugated anti-CD45.1 (A20).
-
bioRxiv - Immunology 2024Quote: ... CD8+ T-cells were isolated from the single cell suspension using the EasySep Mouse CD8+ T-cell isolation kit (Stem Cell Technologies, 19853) and the EasyEights EasySep magnet (Stem Cell Technologies ...
-
bioRxiv - Developmental Biology 2024Quote: ... the tissues were dissociated into single cell suspension using NeuroCult enzymatic dissociation kit for adult mouse and rat CNS tissue (Stem Cell Technologies 05715) and plated on ultra-low attachment 6-well plates (Corning 3471 ...
-
bioRxiv - Immunology 2021Quote: OT1 CD8+ T cells from Wild type or the knockout mice mentioned above were purified from spleens using EasySep™ Mouse CD8+ T Cell Isolation Kit (#19853, STEMCELL Technologies, Vancouver, CA). Isolated CD90.1+ OT1 CD8+ T cells were injected into CD90.2+ C57BL6 recipient mice at a concentration of 106 cells/100ul PBS through the retro-orbital route.
-
bioRxiv - Synthetic Biology 2021Quote: ... in borate buffer for primary culture cells or Poly-D-Lysine for cells from an iPSC background before being coated with either 10 µg/ml mouse laminin or 5 µg/ml human 521 Laminin (Stemcell Technologies Australia, Melbourne, Australia) respectively to facilitate cell adhesion.
-
bioRxiv - Bioengineering 2021Quote: ... Cells were pelleted by centrifugation at 300 g for 10 min and resuspended at 108 cells/mL in EasySep™ buffer and B lineage cells were isolated using an EasySep™ mouse B cell negative isolation kit (StemCell Technologies, #19854). For serum ...
-
bioRxiv - Immunology 2020Quote: CD4+ OT-II cells were isolated from spleen and LNs from OT-II*B6SJL mice with the EasySep mouse CD4+ T cell isolation kit (Stemcell Technologies, Vancouver, BC, Canada), according to manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2021Quote: ... CD45 positive cells were depleted from tumor cell digests using the EasySep ™ Mouse streptavidin Rapidspheres™ Isolation Kit (Stem Cell Technologies, Catalog #19860) and a biotin conjugated anti Mouse CD45 antibody (Stem Cell Technologies ...
-
bioRxiv - Developmental Biology 2021Quote: E14 and its derivative reporter lines pMsgn1-Venus [1] and Hes7-Achilles [2] mouse ESCs were maintained under feeder-free conditions in gelatin-coated dishes (StemCell Technologies cat. no. 07903) with 2i medium composed of high glucose DMEM (Gibco cat ...
-
bioRxiv - Cell Biology 2020Quote: Depletion of lineage-committed cells from single-cell suspensions of mouse bone marrow was performed using the EasySep™ Mouse Hematopoietic Progenitor Cell Isolation Kit according to the manufacturer’s instructions (StemCell Technologies, Vancouver, BC, Canada).
-
bioRxiv - Immunology 2021Quote: ... Cells from all mice were pooled by tissue type and B cells were selected from lymph node and spleen using a mouse Pan-B negative selection kit (Stemcell Technologies, Vancouver, BC, Canada). Generation of natively paired heavy and light chain libraries ...
-
bioRxiv - Immunology 2020Quote: ... B cells were enriched by negative selection using EasySep mouse B cell enrichment kit as per manufacturer’s instructions (StemCell Technologies Inc., Tukwila, WA, USA). Enriched B cells were then resuspended in FACS buffer and stained and sorted as described previously(Carbonetti et al. ...
-
bioRxiv - Immunology 2021Quote: Splenic naïve B lymphocytes were isolated by magnetic bead negative selection using EasySep™ Mouse B Cell Isolation Kit (Catalog no. 19854) from STEMCELL Technologies (Vancouver, BC). B cells were cultured in RPMI 1640 supplemented with 10% FBS ...
-
bioRxiv - Immunology 2024Quote: Mouse B cells were isolated from pooled single cell suspensions of spleen and peripheral lymph nodes using CD19 microbeads (Miltenyi, catalog no. 130-052-201) or EasySep Mouse B Cell Isolation Kit (Stemcell Technologies, catalog no. 19854). B cells were cultured in RPMI1640 medium supplemented with 10% (vol/vol ...
-
bioRxiv - Immunology 2023Quote: ... Intermediate layer containing IELs was collected and washed with 1X PBS followed by either flowcytometry analysis or used for CD8α IELs purification using EasySep™ mouse CD8α positive selection kit-II (StemCell Technologies; Cat# 18953). Sort purified CD8α IELs (50,000/mouse ...
-
bioRxiv - Immunology 2023Quote: ... Purification of CD8+ T cells for sorting or cell culture was performed using a mouse CD8+ T cell isolation kit (Stem Cell Technologies, Cat# 19853), following the manufacturer’s instructions.
-
bioRxiv - Cancer Biology 2023Quote: ... malignant B cells were enriched from splenocytes of Eµ-TCL1 mice using EasySep Mouse Pan-B Cell Isolation Kit (StemCell Technologies, Inc., Cologne, Germany). The CD5+ CD19+ content of purified cells was typically above 95% ...
-
bioRxiv - Immunology 2023Quote: ... CD4 T cells were isolated from the spleen and LNs of indicated naïve OT-II strains with Mouse CD4 T Cell Isolation Kit (STEMCELL Technologies 19852 or BioLegend 480033) and labeled with 1.0 µM CFSE (Thermo Fisher ...
-
bioRxiv - Immunology 2023Quote: Single cell suspensions of splenocytes were prepared and CD4+ T cells were isolated using the EasySep™ Mouse CD4+ T Cell Isolation Kit (StemCell Technologies; Cat # 19852A). CD4+ T cells were plated in a round bottom 96-well plate at 2 × 105 cells/well and activated using Mouse T Activator CD3/CD28 Dynabeads™ (Gibco ...
-
bioRxiv - Cancer Biology 2023Quote: Untreated 2153L and 2151R tumors were dissociated in 1 mg/ml Collagenase A for 2 hrs at 37°C and TAMs were separated using EasySep™ Mouse F4/80 Positive Selection Kit (STEMCELL technologies, #100-0659) following manufacture’s protocol ...
-
bioRxiv - Immunology 2024Quote: CD4+CD25+ T cells isolated from the spleens of DR3 and DR3.IL17A-/- transgenic mice using the EasySep™ Mouse CD4+CD25+ regulatory T Cell Isolation Kit II (STEMCELL Technologies, Vancouver, Canada) according to the manufacturer’s protocols ...
-
bioRxiv - Immunology 2024Quote: ... CD4+CD25+ T cells were isolated from splenocytes of DR3 and DR3.IL17A-/- transgenic mice using EasySep™ Mouse CD4+CD25+ regulatory T cell isolation kit II (STEMCELL Technologies, Vancouver, Canada) according to the manufacturer’s protocols ...
-
bioRxiv - Immunology 2024Quote: ... Dendritic cells (DCs) were isolated from spleens of wild type mice using EasySep™ Mouse Pan-DC Enrichment Kit II (Stem Cell Technologies, 19863). Co-cultures were set up in 4 replicates with BMDM primed as above ...
-
bioRxiv - Immunology 2024Quote: ... OT-I T cells (CD8+) were isolated from spleens of OT I mice using EasySep™ Mouse CD8+ T Cell Isolation Kit (Stem Cell Technologies, 19853A). Dendritic cells (DCs ...
-
bioRxiv - Immunology 2024Quote: ... murine T cells were freshly isolated from NOD.Nr4a1GFP/Cre mice using the EasySep™ Mouse T Cell Isolation Kit (STEMCELL Technologies; Cat. No. 19851) per manufacturer’s protocol ...
-
bioRxiv - Bioengineering 2024Quote: ... disinfected in 70 % ethanol and 40 DRGs from each mouse were dissected and pooled into ice-cold HBSS medium (STEMCELL Technologies, cat. No. 37150). The DRGs from 4 pups were digested for 30 min at 37°C using 13.33 U/ml papain (Worthington ...
-
bioRxiv - Animal Behavior and Cognition 2021Quote: ... splenic T-lymphocytes were negatively selected from total splenocytes through use of EasySep Mouse T-cell Isolation Kit (Stem Cell Technologies #19851, Vancouver, BC, Canada). Samples were validated for >90% viability and purity before subsequent use.
-
bioRxiv - Immunology 2020Quote: ... 1 x 105 T cells purified from spleen and lymph nodes with the EasySep Mouse T cell Isolation Kit (Stem Cell Technologies; Vancouver, BC, Canada) were cultured with 4 x 105 T-depleted splenocytes in 96-well round bottom plates in R10 media with 1 μg/mL αCD3 ...
-
bioRxiv - Immunology 2020Quote: ... CD4T cells were negatively isolated from the digested spleen and LNs of indicated donor mice with Mouse CD4T Cell Isolation Kit (STEMCELL Technologies 19852 or BioLegend 480033) according to the manufacturer’s protocol and labeled with 1.0 μM CFSE (Thermo Fisher ...
-
bioRxiv - Immunology 2024Quote: ILCs from Alt and Alt plus BPC treated mice were enriched from two single-cell suspended lungs by Mouse Pan-ILC Enrichment Kit (STEMCELL Technologies, Vancouver, British Columbia, Canada). ILC enriched samples were preserved in PIPseqTM T100 3L Single Cell Capture and Lysis Kit and processed by sequencing service (Fluent BioSciences ...
-
bioRxiv - Immunology 2024Quote: T cells were extracted from OT-II mouse spleens and magnetically sorted using a T-cell extraction kit from Stem Cell Technologies (catalog number 19851). Cells were incubated in culture media (RPMI+ 10% HIFBS + Pen/Strep + 1 mM HEPES + 50 μM beta mercaptoethanol ...
-
bioRxiv - Immunology 2024Quote: ... splenocytes in a single cell suspension were negatively selected for CD4+ or CD8+ T-lymphocytes using the EasySep Mouse CD4+ T-cell Isolation Kit or CD8+ T-cell Isolation Kit (StemCell Technologies #19852, #19853, Vancouver, BC, USA), respectively ...