Labshake search
Citations for Stemcell Technologies :
501 - 550 of 634 citations for Proteasome 26S Subunit Non ATPase 10 PSMD10 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... For immunolabelling embryos were washed 4 times with PTwH (PBS/0.2% Triton-X-100/10 µg/mL heparin (Stemcell Technologies, #07980)) for 1 h each at 37°C and incubated with primary antibody (PE-labelled rabbit anti-CD31/PCAM1 (1:100 ...
-
bioRxiv - Cell Biology 2023Quote: ... The detached colonies were then pelleted at 300g(av) and reseeded with mTeSR Plus medium supplemented with 10 µM Rock Inhibitor (Y-27632, STEMCELL Technologies). At passage 10 ...
-
bioRxiv - Cancer Biology 2023Quote: Day 5-18 differentiated cells were dissociated with Accutase for 10 min and CD34+ population was purified by magnetic-activated cell sorting (MACS) with an EasySep Magnet kit (STEMCELL Technologies) using a CD34 antibody according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2024Quote: ... the cells were cultured at 37□°C in the presence of 5% CO2 in NeuroCult NS-A Proliferation solution with 10% proliferation supplement (STEMCELL Technologies, Vancouver ...
-
bioRxiv - Neuroscience 2024Quote: ... media was removed and neural induction media (NIM; Supplementary Table 2) supplemented with small molecules 10 µM SB431542 (StemCell Technologies, #72146) and 0.1 µM LDN193189 (StemCell Technologies ...
-
bioRxiv - Cell Biology 2024Quote: ... the cells were resuspended and 50,000-100,000 cells were transferred into fresh matrix-coated plates with mTeSR Plus media containing 10 μM Y-27632 (ROCK inhibitor, STEMCELL Technologies). Fresh media without ROCK inhibitor was fed the next ...
-
bioRxiv - Bioengineering 2023Quote: ... we single cell sorted ATTO550 positive cells for monoclonal expansion in 96-well plates containing their corresponding dTMP-supplemented culture media (hiPSC additionally supplemented with 10% Clone R (StemCell Technologies)) ...
-
bioRxiv - Neuroscience 2024Quote: ... SH-SY5Y cells were counted and seeded at a density of 10.4 × 103 cells/cm2 in differentiation media (Neurobasal medium, 2% B27 supplement, 1% GlutaMAX, and 10 μM of all-trans retinoic acid [STEMCELL Technologies]). Differentiation media was replaced every other day until day 9 post-seeding ...
-
bioRxiv - Bioengineering 2023Quote: ... was prepared by mixing equal volumes of 3mg/mL neutralized collagen with 10% Geltrex and 8×107 cells/mL cell suspension in mTeSR 1(STEMCELL Technologies) containing 10μM ROCK inhibitor Y27632 (Cayman) ...
-
bioRxiv - Neuroscience 2023Quote: ... 50 ng/mL recombinant human FMS-like tyrosine kinase 3 ligand (Flt-3) and 10 ng/mL recombinant human thrombopoietin (TPO; StemCell Technologies). After sublethal conditioning of 9-12 weeks old Gaa−/− mice with 7.5 Gy gamma-irradiation (1.6 Gy/min ...
-
bioRxiv - Cancer Biology 2023Quote: ... and S7 cells were grown as floating spheres and maintained in NeuroCult NS-A Proliferation medium and 10% proliferation supplement (STEMCELL Technologies, Vancouver ...
-
bioRxiv - Neuroscience 2022Quote: ... Medium was replaced every other day and cells were passaged ~1:10 onto fresh Matrigel-coated vessels using ReLeSR (StemCell Technologies) when 90% confluent.
-
bioRxiv - Cancer Biology 2022Quote: ... Patient samples were cultured in RPMI with 10% FBS and 10% HS-5 conditioned media (ATCC) or SFEMII supplemented with 1x StemSpan CD34+ Expansion Media and 1 µM UM729 (StemCell Technologies).
-
bioRxiv - Cell Biology 2022Quote: ... Cells were then passaged using GCDR and replated on 10-cm dishes coated with Animal Component Free (ACF) Attachment Substrate (StemCell Technologies); all subsequent passages were grown using the same medium and culture substrate ...
-
bioRxiv - Cell Biology 2022Quote: ... Dissociated cells were plated on a Matrigel or Culturex coated culture plate and cultured in 10 uM Y27632 (72304, StemCell Technologies) containing mTeSR1 one day before differentiation ...
-
bioRxiv - Neuroscience 2024Quote: ... After nucleofection cells were plated onto a 10 cm dish coated with Synthemax (5 μg/cm2) with TeSR-E8 supplemented with CloneR (StemCell Technologies). Cells were cultured to 80% confluency ...
-
bioRxiv - Neuroscience 2024Quote: ... and seeded on Geltrex-coated plates in STEMdiff Neural Induction Medium supplemented with SMADi and 10 nM Y-27632 (a ROCK inhibitor) (all reagents from STEMCELL Technologies). For further cultivation ...
-
bioRxiv - Molecular Biology 2024Quote: ... NIM is composed of NBM supplemented with small molecules SB431542 (10 µM, an inhibitor of TGFβ pathway) (72232, Stem Cell Technologies) and LDN193189 (0.1 µM ...
-
bioRxiv - Immunology 2024Quote: The various culture media were enriched with the following supplements: kinase inhibitors targeting the TGFβ pathway, including SB431542 (SB, 10 μM) (STEMCELL technologies) and LY364947 (LY ...
-
bioRxiv - Immunology 2024Quote: ... Sample volume was brought to 5 mL and test tubes were placed in a magnetic block for 10 min (“The Big Easy” EasySep magnet, cat. no. 18001, STEMCELL Technologies). Tubes were gently rocked to allow F4/80+ ATM to be magnetically pulled towards the wall of the test tubes ...
-
bioRxiv - Neuroscience 2024Quote: ... A 10 µM 2X working solution of CalbryteTM AM dye was prepared in sterile BrainPhysTM Imaging Optimized Medium (BPI, STEMCELL Technologies) containing 0.08% pluronic F-127 (#20050 ...
-
bioRxiv - Cell Biology 2024Quote: ... IL-3/IL-6 and SCF cultures:3 Equal volume of medium was added to each well to a final concentration of 10% FBS (STEMCELL Technologies), 1% penicillin/streptomycin (Sigma-Aldrich) ...
-
bioRxiv - Cell Biology 2024Quote: ... For ECM concentration experiments and mechanical actuation experiments on Mechanoculture FX2 plates fibronectin coating (1 µg/mL and 10 µg/mL, in PBS StemCell Technologies) at 37°C for 1 hour were used.
-
bioRxiv - Cell Biology 2024Quote: ... IL-11 and SCF cultures:2 Equal volume of medium was added to each well to a final concentration of 10% FBS (STEMCELL Technologies), 1% penicillin/streptomycin (Sigma-Aldrich) ...
-
bioRxiv - Immunology 2024Quote: ... single cell suspensions harvested from cell culture or isolated from spleen or mediastinal lymph nodes were incubated with 10% Rat serum (STEMCELL Technologies), Fixable Viability Dye (ebioscience ...
-
bioRxiv - Bioengineering 2024Quote: ... and reseeded as a single cell suspension at a density of 1.5×104 cells/cm in mTeSR™ Plus supplemented with 10 μM Y-27632 RHO/ROCK pathway inhibitor (72304, STEMCELL Technologies). Media was replaced with Y-27632 free mTeSR™ Plus after 24 hours.
-
bioRxiv - Bioengineering 2024Quote: ... and seeded as single cells (density of 1.35 × 105 cells/cm2) in mTeSR1 medium supplemented with 10 µM Rho-Associated kinase inhibitor (Stem Cell Technologies). The pancreatic differentiation was done following previously described protocols36,38,59 ...
-
bioRxiv - Bioengineering 2024Quote: ... media on the PSCs was changed to fresh mTeSR Plus supplemented with 10 µM ROCK inhibitor Y-27632 (Stem Cell Technologies). For the CASQ2-/- mutation ...
-
bioRxiv - Bioengineering 2024Quote: ... Tissues were dissected into small sections and rubbed on a serrated Petri dish containing 10 mL of an enzyme cocktail comprising 0.5 mg/ mL Collagenase Type IV (Stem Cell Technologies), 0.5 mg/ mL Liberase (Sigma-Aldrich ...
-
bioRxiv - Cancer Biology 2021Quote: ... the cells were incubated with specific neuronal marker CD24-FITC Clone M1/69 antibody(38) (Stemcell Technologies, Canada) diluted 1:100 for 30 minutes on ice ...
-
bioRxiv - Bioengineering 2021Quote: ... hiPSCs were thawed into 6 well plates (TPP) that were pre-coated for 1h at RT with 10 μg/ml Vitronectin (Stem Cell Technologies) in CellAdhere dilution buffer (Stem Cell Technologies) ...
-
bioRxiv - Neuroscience 2020Quote: ... the glucose concentration of LN was adjusted to 10 mM and the following supplements were added freshly before use: 20 ng/ml BDNF (Stem Cell Technologies), 20 ng/ml GDNF (Stem Cell Technologies ...
-
bioRxiv - Cancer Biology 2020Quote: ... 2×10^4 cells from bone marrow of xenotransplanted mice were transferred to 2ml of MethoCult H4034 optimum methylcellulose medium (Stem Cell Technologies) and plated onto two 35mm dishes ...
-
bioRxiv - Cell Biology 2020Quote: ... the imaging chamber was incubated overnight at 4° C with 10 μg/mL of a mouse monoclonal anti-CD3 (clone UCHT1, StemCell Technologies, #60011) in PBS ...
-
bioRxiv - Microbiology 2022Quote: ... HCT-8 cells were cultured in 25 cm flasks with 5 ml of complete media (RPMI-1640 media [Gibco/Thermo Fisher Scientific, Waltham, MA] supplemented with 10% fetal bovine serum [Stemcell Technologies, Vancouver ...
-
bioRxiv - Neuroscience 2021Quote: ... iPSCs were plated 48 hrs later at very low density (10 cells/cm2) on Laminin-521 with CloneR supplement (Stem Cell Technology) for clonal selection ...
-
bioRxiv - Bioengineering 2021Quote: 5 ×10 3 Floating cells collected at different time points (day 8, day 10, and day 12) were grown in 1 mL of cytokine containing MethoCult H4434 medium (StemCell Technologies, Vancouver) at 37° C ...
-
bioRxiv - Developmental Biology 2020Quote: ... Cells were passaged in clumps of 8-10 cells and seeded in mTeSR supplemented with the Rho-associated protein kinase inhibitor γ-27632 (STEMCELL Technologies) at 10 µM for the first day to improve survival ...
-
Prevention of tau accumulation through inhibition of hnRNP R-dependent axonal Mapt mRNA localizationbioRxiv - Neuroscience 2023Quote: ... Alomone Labs), 10 ng/mL brain-derived neurotrophic factor (167450-02-STD, tebu-bio) and 500 µM dibutyryl-cAMP (Stemcell Technologies, 73886) and motoneurons were maintained for 25 DIV.
-
bioRxiv - Immunology 2023Quote: ... BLaER1 cells were trans-differentiated into Mo for 6-7 days in cRPMI medium containing 10 ng/mL of hrIL-3 (Stem Cell Technologies), 10 ng/mL hrM-CSF (Stem Cell Technologies ...
-
bioRxiv - Cancer Biology 2024Quote: MDA-MB-468 and mitomycin-C treated NIH-3T3 cells were plated at a ratio of 10:1 in AggreWellTM400 plates at approximately 50 cells/microwell according to the manufacturer’s instructions (#34415, Stemcell Technologies, Vancouver, BC) and grown for 5 days in tumor media containing 1μg/mL doxycycline ...
-
bioRxiv - Neuroscience 2024Quote: ... Embryoid body media (EBM) was used for days 2 to 11 (Supplementary Table 2) and supplemented with 10 µM SB431542 (Stemcell Technologies, #72234), 0.1 µM LDN193189 (MedChemExpress ...
-
bioRxiv - Cancer Biology 2023Quote: ... Matrigel was allowed to polymerize at 37 °C for 10-15 minutes before mouse IntestiCult Organoid Growth Medium (STEMCELL Technologies cat#06005), containing Y-27632 (10 μM) ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Cells were plated into 96 well flat bottom plates in growth medium supplemented with 10 µM of ROCK inhibitor (Stem Cell Technologies). Cells were plated at low density (2,500 cells/well ...
-
bioRxiv - Developmental Biology 2022Quote: ... EBs were embedded in Matrigel and transferred to 10-cm cell-culture dishes coated with anti-adherence rinsing solution (Stemcell technologies, P2443) in 15ml NI ...
-
bioRxiv - Bioengineering 2023Quote: ... Single cells were centrifuged at 200g for 5 minutes and resuspended in StemFlex supplemented with 10 µM Y-27632 (StemCell Technologies Inc.). We seeded 5.5×106 cells per 5.5 mL of mTeSR+ in each well of a six-well suspension plate ...
-
bioRxiv - Cell Biology 2024Quote: ... cells were replated in mTeSR1 supplemented with 1% P/S and 10 μM ROCK inhibitor (Y-27632, STEMCELL Technologies, Vancouver, BC, Canada) for 24 h ...
-
bioRxiv - Immunology 2024Quote: ... using a pre-coated serological pipette until the supernatant was clear of intestinal contents and mucus (10-15 times) and incubated with Gentle Cell Dissociation Reagent (STEMCELL Tech, Canada) for 15 min at room temperature (RT ...
-
bioRxiv - Cell Biology 2024Quote: ... into the protein coated 96-well plates containing 100 µl intestinal organoid growth media supplemented with 10 µM Y-27 (Stem cell technologies). Growth of colonies were analysed using brightfield and fluorescent images with EVOS microscope ...
-
bioRxiv - Cancer Biology 2024Quote: ... washed with DMEM (centrifuged at 300 g for 10 mins at 4°C) and further dissociated into single cells using the NeuroCult Chemical Dissociation Kit (STEMCELL Technologies; #05707) in accordance with the manufacturer’s instructions ...