Labshake search
Citations for Stemcell Technologies :
501 - 550 of 1007 citations for L Alanine N T Boc 2 13C 98 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... T-cell cultures were fed with fresh 10% RPMI supplemented with human IL2 (20u/ml Stem Cell Technologies) every 2-3 days to maintain 1×106 cells/mL/mm2 ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T cells were obtained by negative selection using the EasySep Human CD4+ Enrichment kit (Stem Cell Technologies), and stimulated with anti-CD3-coated magnetic beads (Dynabeads M-450 tosyl-activated ...
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bioRxiv - Immunology 2022Quote: ... eNK cells added to T cell cultures were removed (EasySep Human CD56 Positive Selection Kit II, STEMCELL Technologies) prior to analysis ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were then activated for 3 days in “Complete media” (Immunocult-XF T cell expansion media (STEMCELL Technologies), CTS Immune Cell serum replacement (Fisher Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: CD8+ cells were enriched from human PBMCs with EasySep Human CD8+ T Cell Isolation Kit (Stem Cell Technologies) and stimulated with 5 ug/mL Ultra-LEAF anti-human CD3 (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... naive Treg cells were purified by using EasySep Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit (STEMCELL Technologies). For human cell culture ...
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bioRxiv - Molecular Biology 2023Quote: ... starting from freshly isolated PBMCs from 69 PLWH using the EasySep CD4 T cell isolation kit (Stemcell Technologies). CD4+ T cells were frozen as dry pellet at −80°C prior to DNA extraction with the DNeasy Blood & Tissue kit (Qiagen) ...
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bioRxiv - Immunology 2023Quote: ... For Il-2ra time course naïve CD4+ T cells were enriched from splenocytes using EasySep kit (StemCell Technologies). Approximately 100,000 cells were activated using 2ug/ml plate bound anti-CD3/CD28 ...
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bioRxiv - Cancer Biology 2023Quote: ... at 2.5 x 106 cells/mL and ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator (STEMCELL Technologies) at 25μL/mL ...
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bioRxiv - Immunology 2023Quote: Splenic B cells or T cells were Isolated with EasySepTM Mouse B Cell Isolation Kit (#19854, StemCell Technologies) or EasySepTM Mouse T Cell Isolation Kit (#19851 ...
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bioRxiv - Microbiology 2024Quote: Primary human CD4+ T cells from healthy donors were isolated from leukoreduction chambers after Trima apheresis (STEMCELL Technologies). PBMCs were isolated by Ficoll centrifugation ...
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bioRxiv - Developmental Biology 2021Quote: ... Definitive endoderm cells are then differentiated into anterior ventral foregut endoderm (Stage 2) using dual SMAD inhibition for 2 days with 2 µM Dorsomorphin (STEMCELL Technologies, cat#72102) and 10 µM SB431542 (STEMCELL Technologies ...
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bioRxiv - Biophysics 2023Quote: ... with the addition of Human Recombinant Interleukin 2 (IL-2, STEMCELL Technologies Inc., USA).
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bioRxiv - Cancer Biology 2023Quote: ... 100 ng/ml SCF and 10 ng/ml Flt3-L (Stem Cell Technologies). 1 x 106 transduced HSPCs were resuspended in PBS and injected into the tail vein of 6-week-old female Balb/c mice ...
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bioRxiv - Cell Biology 2022Quote: ... CD4+ cells were isolated by negative selection (RosetteSepTM Human CD4+ T cell Enrichment Cocktail, STEMCELL technologies, Cambridge, UK; #15023) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Murine CD4 T cells were isolated from processed lymphoid organs using EasyEights magnet (Stem Cell Technologies, Catalog no. 18103) and the whole CD4+ T cell isolation kit (Stem Cell Technologies ...
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bioRxiv - Microbiology 2020Quote: ... 5×106 naïve CD4+ T cells from HIV− or HIV+ donors were isolated by magnetic negative selection (StemCell Technologies), then cultured at 106 cells/mL in 96-well plates using R-10 media (RPMI-1640 media supplemented with 10% FBS ...
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bioRxiv - Bioengineering 2020Quote: ... T cells were frozen at 10–20 × 106 cells per 1 mL of Cryostor CS10 (STEMCELL Technologies, Cambridge, MA) and thawed into culture for knockout experiments.
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bioRxiv - Immunology 2020Quote: CD4 or CD8 T cells were isolated from spleen using magnetic beads as described by manufacturer’s protocol (STEMCELL technologies). 4000 cells in ratio 1:1 were transferred into WT mice 1 day prior to LCMV-Cl13 infection.
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bioRxiv - Immunology 2020Quote: Total or naïve mouse CD4+ T cells were isolated from the spleen or mesenteric LNs by negative selection with the EasySep Mouse CD4+ T cell enrichment kit or the EasySep Mouse naïve CD4+ T cell enrichment kit according to the manufacturer’s protocol (Stemcell Technologies). In certain cases ...
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bioRxiv - Immunology 2020Quote: ... and used directly for total T cell enrichment by negative magnetic selection using Easysep magnetic separation kits (STEMCELL Technologies). Cell suspension was stained with anti-CD4 (RPA-T4) ...
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bioRxiv - Immunology 2021Quote: ... and CD4+ T cells were pre-activated with either CD3/CD28/CD2 tetrameric antibody complexes (ImmunoCult by STEMCELL Technologies) or 1:1 aAPCs for 5 d ...
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bioRxiv - Immunology 2021Quote: ... Bulk T cells were isolated from Leukopaks using EasySep magnetic selection following manufacturers’ recommended protocol (Stemcell Technologies cat 17951). Immediately following isolation ...
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bioRxiv - Immunology 2021Quote: ... 5mL of whole blood was enriched for CD4+ cells using RosetteSep Human CD4+ T Cell Enrichment Cocktail (StemCell Technology). CD4+ cells were frozen in 10% DMSO/FBS and stored in liquid nitrogen ...
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bioRxiv - Immunology 2020Quote: ... Isolated CD3+ cells and tumor-infiltrating lymphocytes (TILs) were maintained in Human T Cells Expansion (STEMCELL Technologies, Vancouver, Canada) medium supplemented with 5% of heat-inactivated human AB serum (Omega Scientific ...
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bioRxiv - Immunology 2022Quote: ... naïve CD8+ T cells were purified from spleen and peripheral lymph nodes by negative selection (StemCell Technologies, Vancouver, BC) as previously described (2) ...
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bioRxiv - Immunology 2022Quote: ... CD8+ T cells were isolated from splenocytes of OT-I mice using a magnetic bead isolation kit (Stemcell Technology) and cultured following a previously published protocol (44) ...
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bioRxiv - Biochemistry 2022Quote: T cells were isolated from peripheral blood mononuclear cells (PBMCs) using the RoboSep-S cell isolation platform (STEMCELL Technologies) with EasySep Human T cell Isolation Kit (STEMCELL Technologies) ...
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bioRxiv - Microbiology 2023Quote: Phosphorylation status of p65 and c-JUN were analyzed by flow cytometry in isolated CD4 T cells (StemCell Technologies) as previously described (44) ...
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bioRxiv - Immunology 2023Quote: ... and CD4+ cells were magnetically enriched using mouse negative selection CD4+ T cell isolation kits (STEMCELL Technologies and Biolegend). Live Tregs were sorted as fixable viability dyeneg (Thermo Fisher Scientific and Tonbo Biosciences) ...
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bioRxiv - Biophysics 2023Quote: Primary human CD4+ T cells were isolated from commercially available whole human blood (STEMCELL Technologies Inc., USA, Cat# 70507.1) with EasySep Human CD4+ T Cell Isolation Kits (STEMCELL Technologies Inc. ...
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bioRxiv - Cell Biology 2023Quote: ... CD4+ T cells were isolated from monocyte-depleted PBMCs using EasySepTM Human CD4+ Selection Kit II (StemCell Technologies Ltd.) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... Human pan T cells were purified from frozen peripheral blood mononuclear cells (PBMCs, STEMCELL Technologies, Cat #70025.2. Lot 201671701C) using a negative selection human Pan T Cell Isolation Kit (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% heat-inactivated FCS and stimulated with Immunocult™ Human CD3/CD28 T cell Activator (Stemcell Technologies) (25 µL/ 106 cells ...
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bioRxiv - Immunology 2023Quote: ... APCs from NOD spleens were obtained by depleting T cells using the Mouse CD90.2 Positive Selection Kit (STEMCELL Technologies) then pulsed with 15 µM of the indicated peptides and co-cultured with Tregs/Tconvs at a 1:1 ratio ...
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bioRxiv - Immunology 2023Quote: Transduced T cells were depleted of CD56 cells using the EasySep Human CD56 positive selection kit (Stemcell Technologies; 17855) and rested for 24 hours ...
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bioRxiv - Bioengineering 2023Quote: ... cells were cultured in collagen-I gels in the ImmunoCult-XF T Cell Expansion Medium (STEMCELL Technologies, Cat# 10981) with the addition of Human Recombinant Interleukin 2 (STEMCELL Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... and Naïve CD4+ T cell were isolated from splenocytes with commercial kits (STEMCELL Technologies, Cat No. 19852, 19853, 19765) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Naïve CD8 T cells were purified to >90% from the spleens of donor strains using negative selection (StemCell Technologies) and stained with CTV or CTFR proliferation dyes (Molecular Probes ...
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bioRxiv - Immunology 2023Quote: ... naïve CD45.1/CD45.2+ P14+ CD8+ T cells were purified from spleens using the StemCell CD8+ isolation kit (STEMCELL Technologies) and 500 were adoptively transferred into CD45.1+ mice ...
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bioRxiv - Immunology 2024Quote: CD8+ T cells from Thy1.1+ P14 mice (PBMCs) were enriched using a CD8 MACS-negative selection kit (STEMCELL Technologies). ∼35,000 P14 CD8+ T cells were transferred intravenously into naïve Thy1.2+ C57BL/6 recipient mice ...
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bioRxiv - Immunology 2024Quote: ... with 5×103 splenic P14 TN cells congenic for CD90.1+ or CD45.1+ isolated by negative selection using the EasySep Mouse CD8+ T cell isolation kit (STEMCELL Technologies) before LCMV Arm infection eight hours later ...
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bioRxiv - Bioengineering 2019Quote: ... or APEL 2 (STEMCELL Technologies) media without ROCK inhibitor and plates were placed onto LAVA illumination devices and subjected to experimental conditions ...
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bioRxiv - Neuroscience 2021Quote: ... 2 µM XAV939 (STEMCELL Technologies), 10 µM SB431542 hydrate and 2 µg/mL doxycycline hyclate (Sigma) ...
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bioRxiv - Pathology 2022Quote: ... 2 µM CHIR99021 (StemCell Technologies), and 10 ng/mL Recombinant Human BMP-7(Peprotech) ...
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bioRxiv - Cell Biology 2022Quote: ... 2% SM1 (Stem Cell Technologies), 15mM HEPES (Gibco) ...
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bioRxiv - Neuroscience 2023Quote: ... IWP-2 (STEMCELL Technologies, 72124), RA (Sigma ...
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bioRxiv - Developmental Biology 2023Quote: ... 2 μM SB431542 (StemCell Technologies), 1 μM DMH1 (Tocris Bioscience) ...
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bioRxiv - Bioengineering 2023Quote: ... (StemCell Technologies, #129830-38-2) in mTeSR-1 media ...
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bioRxiv - Bioengineering 2023Quote: ... (StemCell Technologies, #129830-38-2) in mTeSR-1 media ...