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Citations for Stemcell Technologies :
501 - 550 of 4374 citations for Dengue Virus Serotype 2 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: CD8+ T lymphocytes were enriched from lung cell suspensions prepared according to the manufacturer’s instructions with the CD8+ T cell isolation kit (Stem Cell Technologies). Purified CD8+ T cells were incubated in ELISpot plates in the presence of medium or a pool of S1 peptides (1 μg/mL ...
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bioRxiv - Molecular Biology 2023Quote: ... Cardiomyocyte/stromal cell cultures were produced from HES3 human embryonic stem cells (hESCs, WiCell) maintained using mTeSR-1 (Stem Cell Technologies). hESCs were seeded at 2×104 cells/cm2 in Matrigel coated flasks and cultured for 4 days using mTeSR-1 and passaged with TrypLE (ThermoFisher) ...
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bioRxiv - Biochemistry 2023Quote: Cells were counted and adjusted to a concentration of 5 x 105 cells/mL with ALDEFLUOR assay (01700; Stem Cell Technologies) buffer ...
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bioRxiv - Bioengineering 2023Quote: ... ∼80% confluent human induced pluripotent stem cells (hPSCs) were dissociated into single cells with the Gentle Cell Dissociation Reagent (STEMCELL Technologies). Bioink consisting of 1.5mg/mL collagen with 5% Geltrex and high-density hPSCs (4×107 cells/mL ...
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bioRxiv - Immunology 2023Quote: ... Naive B cells were isolated by negative magnetic activated cell sorting using the EasySepTM Mouse B cell Isolation Kit (StemCell Technologies). 30 million B cells were injected i.p ...
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bioRxiv - Immunology 2023Quote: ... B cell purification was performed according to manufacturer instructions with the Stem Cell EasySep Human Pan-B Cell Enrichment kit (Stemcell Technologies). After enrichment ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... human MM cells were cultured at a density of 1 × 104 cells in MethoCult™ H4230 media (Stem Cell Technologies, #04230) supplemented with 15% FBS and 10% Gibco Phytohemagglutinin M form (PHA-M ...
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bioRxiv - Cell Biology 2024Quote: ... mature NK cells were isolated from peripheral blood mononucleated cells with the EasySep™ Human NK Cell Enrichment Kit (STEMCELL Technologies). The human mesenchymal stromal cells (h-MSC ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells were cultured for 72 hours in 96-well plates (2X105-5X105 cells per well) containing StemPro (Stem Cell Technologies) media supplemented with human IL-6 ...
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bioRxiv - Developmental Biology 2023Quote: ... and kept on Rho-associated protein kinase (ROCK) inhibitor Y-27632 (final concentration 5 μM, StemCell Technologies, #72302) for one day ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 2% FBS and 1.5ug/mL anti-human CD32 antibody (Clone IV.3; STEMCELL Technologies). Cells were stained for 20-30 minutes on ice with 1:200 anti-CD11b-BV711 (Clone M1/70 ...
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bioRxiv - Genomics 2020Quote: ... Tissues were minced into ~1-2 mm pieces and digested with 1× Collagenase/Hyaluronidase (STEMCELL Technologies) and 100 μg/mL DNase I (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2020Quote: ... Dual SMAD inhibitors (2μM Dorsomorphin; StemCell Tech; #72102, and 2 μM A83-01; StemCell Tech; #72022) until day 5 ...
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bioRxiv - Neuroscience 2021Quote: ... with the addition of the following supplements 2% (v/v) NeuroCultTM SM1 Neuronal Supplement (StemCell Technologies), 1% (v/v ...
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bioRxiv - Developmental Biology 2022Quote: ... Half well equivalent was seeded into 2 ml of methylcellulose medium (Human enriched H4435, Stemcell Technologies). Cells were incubated in the assay for 14 days and then scored.
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bioRxiv - Genomics 2021Quote: Tissues were minced into ~1-2 mm pieces and digested with 1× Collagenase/Hyaluronidase (STEMCELL Technologies) and 100 μg/mL DNase I (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... on day 2 media was changed to Neurobasal A and DMEM/F12 1:1 (Stemcell technologies) supplemented with N2 0.5% ...
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bioRxiv - Developmental Biology 2023Quote: ... with 6 μM CHIR99021 in 1.2 mL STEMdiff™ APEL™2 medium (STEMCELL Technologies, # 05270) at the bottom of the chamber for 1-hour ...
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bioRxiv - Developmental Biology 2023Quote: ... The following day the medium was replaced with STEMdiff™ APEL™2 (StemCell Technologies, #05270), containing 8 μM CHIR-99021 (Tocris ...
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bioRxiv - Cell Biology 2023Quote: ... medium was changed to 2 mL of complete STEMdiffTM Astrocyte Differentiation Medium (ADM) (Stemcell Technologies, #08540). On day7 and day 14 ...
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Understanding immune-driven brain aging by human brain organoid microphysiological analysis platformbioRxiv - Bioengineering 2022Quote: Human embryonic stem cells were dissociated into single cells using ReLeSR (Stemcell Technologies) treatment ...
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bioRxiv - Cell Biology 2022Quote: ... cryopreserved CD4+ human cord blood T cells were purchased from Stem Cell Technologies. T cells were isolated from mixed T and B cell cultures using nylon wool separation96 ...
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bioRxiv - Immunology 2021Quote: ... Red blood cells and dead cells were eliminated through Lymphoprep (StemCell Technologies, Canada) density gradient centrifugation ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were routinely passaged as clumps using gentle Cell Dissociation Reagent (Stemcell Technologies) approximately every 3-4 days ...
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bioRxiv - Developmental Biology 2019Quote: H9 cells (WiCell, SLA# 16-W0026) were cultured in mTESR1 (Stem Cell Technologies). Cultured cells were replenished with fresh media every day ...
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bioRxiv - Biophysics 2019Quote: ... Cells were passaged by dissociation with Neurocult Chemical Dissociation Kit (Stem Cell Technologies). For immunostaining ...
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bioRxiv - Genomics 2020Quote: ... cells were treated with 10 μM ROCK Inhibitor (Y-27632) (Stem Cell Technologies) in Essential 8 (E8 ...
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bioRxiv - Immunology 2021Quote: Cells were sequentially enriched for CD4+ and CD25+ cells by RosetteSep (STEMCELL Technologies) and CD25 MicroBeads II (Miltenyi Biotec) ...
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bioRxiv - Cancer Biology 2021Quote: ... The strained cell suspension was serially diluted in Mammocult media (Stem Cell Technology) and seeded at 1 cell/well in ultra-low adherence round bottom 96-well plates (Corning ...
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bioRxiv - Genomics 2021Quote: ... Cultures were expanded weekly with Gentle Cell Dissociation Buffer (GCDR, Stem Cell Technologies) at 70-90% confluency at a 1:10 ratio ...
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bioRxiv - Bioengineering 2021Quote: ... Negative selection using the RosetteSep Human T cell Enrichment kit (Stem Cell Technologies) and SepMate-50 tubes was performed to purify primary human T cells ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were passaged by incubation in Gentle Cell Dissociation media (Stemcell Technologies, 07174) for 4 minutes at 37°C to obtain single cells or RT for 6 minutes to obtain small aggregates of colonies ...
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bioRxiv - Developmental Biology 2022Quote: ... iCRISPR 409-B2 cells were cultivated in mTeSR Plus (Stem Cell Technologies, 05825) supplemented with 100 U/ml P/S on Matrigel-coated T75 tissue culture flasks ...
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bioRxiv - Cell Biology 2022Quote: ... Immature cells were expanded in StemSpan serum free medium (SFEM; Stem Cell Technologies) supplemented with 50 ng/ml human stem cell factor (hSCF ...
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bioRxiv - Neuroscience 2021Quote: ... hiPSCs were dissociated to single cells with Accutase (Stem Cell Technologies, Cat.No. 07920) and plated at 3 × 106 cells/well to allow the embryoid body (EB ...
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bioRxiv - Cell Biology 2020Quote: ... cells were maintained in mTeSR media with supplement (Stem Cell Technologies, Cat.#05850) at 37° C with 5% CO2 ...
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bioRxiv - Cancer Biology 2020Quote: Cancer stem cell activity was measured using the ALDEFLUOR assay (Stem Cell Technologies). This fluorescent reagent system allows detecting the activity of aldehyde dehydrogenase (ALDH) ...
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bioRxiv - Genomics 2020Quote: ... ES cells were grown on 0.1% gelatin-coated culture dishes (Stem Cell Technologies).
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bioRxiv - Cancer Biology 2021Quote: ... with the addition of mouse cell proliferation supplement (Stem Cell Technologies, Cat #05701), PS ...
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The transcription factor RUNX2 drives the generation of human NK cells and promotes tissue residencybioRxiv - Immunology 2022Quote: ... CD3-CD19- mononuclear cells were enriched by Lymphoprep (Stem Cell Technologies, Grenoble, France) density gradient centrifugation followed by Magnetic-activated cell sorting using biotin MicroBead kit (Miltenyi Biotec ...
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bioRxiv - Developmental Biology 2019Quote: ... hESCs colonies were dissociated into single cell suspension using accutase (Stem cell technologies). 9000 cells per well were seeded in an ultra-low attachment round bottom 96-well plate (Corning ...
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bioRxiv - Cell Biology 2020Quote: ... Mononuclear cells were isolated over a Ficoll gradient (Lymphoprep: Stem Cell Technologies, 7851) prior to separation into CD34+ and flowthrough (CD34− ...
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bioRxiv - Physiology 2021Quote: ... Intestinal epithelial cells were dissociated using a Gentle Cell Dissociation Reagent (Stemcell Technologies) and were filtered through a 70 µm cell strainer to remove villi ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Single-cell passaging medium by adding Y-27632 (10 μM, Stem Cell Technologies) to mTeSR1 was used for the 1st day of feeder-free culture (Watanabe et al. ...
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bioRxiv - Developmental Biology 2022Quote: ... the cells were transferred into STEMdiffTM Neural Progenitor Medium (Stem Cell Technologies, #05833) for expansion and freezing.
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bioRxiv - Physiology 2022Quote: ... cells were expanded for 3 days in StemSpan medium (Stem cell Technologies Inc) supplemented with 5% fetal bovine serum (FBS) ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were lifted from the plate using ReLeSR (Stem Cell Technologies; 100-0484) and hiPSCs were spun for 5min at RT and 135g ...
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bioRxiv - Neuroscience 2022Quote: ... Passaged cells were kept in media containing 10µM Y-27632 (Stem Cell Technologies) for 24 hours to promote survival ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Cells were dissociated into single cell suspensions by Accutase treatment (Stemcell Technologies, 07922) for 30-60 min ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cells were dissociated into single cell suspensions by Accutase treatment (Stemcell Technologies, 07920) for 30 minutes at 37 °C ...