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Citations for Stemcell Technologies :
451 - 500 of 706 citations for Recombinant Mouse Regenerating Islet derived 3 Alpha His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... 3-month-old and 18-month-old WT C57BL/6 mice were lineage depleted using Mammary Epithelium Enrichment Kit (Stem Cell Technologies) and resuspended in injection media (HBSS +2% FBS+1% PSA+50% Matrigel ...
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bioRxiv - Developmental Biology 2023Quote: PP cells were dissociated using TrypLE Express at 37°C for 2-3 mins and seeded in microwells of AggreWell 800 plates (STEMCELL Technologies, 34825), using the recommended procedure by the supplier ...
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bioRxiv - Cancer Biology 2023Quote: ... Organoids were passaged every 3-4 days by dissolving the Matrigel in Gentle Cell Dissociation Reagent (GCDR - STEMCELL Technologies cat#100-0485). Organoids were broken down to smaller sizes by trituration in GCDR before washing with basal media (advanced DMEM/F12 ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were checked daily for differentiation and were passaged every 3-4 days using Gentle cell dissociation solution (STEMCELL Technologies, cat # 07174). All experiments were performed under the supervision of the Vanderbilt Institutional Human Pluripotent Cell Research Oversight (VIHPCRO ...
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bioRxiv - Cell Biology 2024Quote: ... we changed the medium to a 1:1 mixture of KSR and N2B medium (DMEM F12, Thermo Fisher 11320033, with 1% GlutaMAX, 3% dextrose, N2-Supplement B, StemCell Technologies 07156 ...
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bioRxiv - Immunology 2024Quote: Vascular immune organoids (VIOs) were washed with PBS 3 times and were dissociated by 30 mins incubation of accumax (StemCell Technologies, 07921). Cells were washed once and resuspended in 100μl blocking buffer (2%FBS and 1% FcX (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... isolated T-IEL were further enriched using an EasySep™ Mouse CD8α positive selection kit (STEMCELL technologies) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... B cells were purified using the EasySep negative selection mouse B cell enrichment kit (Stem Cell Technologies). B cell purity was assessed by flow cytometry using anti CD19 or B220 antibodies (we routinely observed over 98% purity) ...
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bioRxiv - Immunology 2019Quote: ... hMT and s-hMT mice were purified with the EasySep Mouse B Cell Isolation Kit (Stemcell Technologies). B cells were cultured for 2 to 4 days in RPMI 1640 with UltraGlutamine (Lonza ...
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bioRxiv - Genomics 2020Quote: ... and lineage negative cells were isolated using the EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (STEMCELL Technologies).
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bioRxiv - Immunology 2019Quote: ... and NK cells were purified by negative selection using EasySep Mouse NK Cell Isolation Kit (STEMCELL TECHNOLOGIES). Normalized NK-cell numbers (1–3 × 106 ...
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bioRxiv - Immunology 2021Quote: T cells were isolated from disaggregated synovium with the EasySep Mouse T cell isolation kit (STEMCELL Tech) as per manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... T cells were purified by negative selection with the EasySep Mouse T cell isolation kit (STEMCELL Tech) as per manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: NK cells from the spleen were enriched by the EasySep Mouse NK cell isolation kit (STEMCELL Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Our murine neurosphere media consisted of 45 ml Neurocult Mouse Basal media (Stem Cell Technologies, Catalog# 05700), 5 ml of mouse Neurocult Proliferation Supplement (Stem Cell Technologies ...
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bioRxiv - Immunology 2022Quote: ... negative selection using the EasySep Mouse T Cell or CD8+ T cell Isolation Kit (STEMCELL Technologies #19853) was used to isolate CD8+ T cells ...
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bioRxiv - Cell Biology 2022Quote: ... mouse embryonic fibroblasts (MEF) and HeLa cells were cultivated in Dulbecco’s modified Eagle’s medium (DMEM) (Stemcell technologies) supplemented with 10% fetal bovine serum (Life Technologies ...
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bioRxiv - Immunology 2020Quote: ... T cell isolation was performed using EasySep mouse negative T cell isolation kit (Stemcell technologies, Cat # 19851) according to manufacturer’s guidelines ...
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bioRxiv - Immunology 2020Quote: Intestinal crypts were dissociated from mouse small intestine using Gentle Cell Dissociation Reagent (Stemcell Technologies, Cambridge, UK). The crypts were then re-suspended in Intesticult medium (Stemcell Technologies ...
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bioRxiv - Microbiology 2019Quote: Pretumor splenic B cells were purified using the Mouse Pan-B Cell Isolation Kit by StemCell Technologies. Bone marrow cells were flushed from femurs and tibia ...
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bioRxiv - Immunology 2020Quote: ... were purified via negative selection (EasySep™ Mouse CD4+ T cell Isolation Kit, STEMCELL Technologies, Lot: 18H94653). Purified CD4+ T cells were cultured for 72 hours with interleukin-12 (IL-12 ...
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bioRxiv - Immunology 2019Quote: ... Human CD45+ cells were enriched using the EasySep™ Mouse/Human Chimera Isolation Kit (Stemcell technologies, 19849) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... and CD8+ T cells were negatively selected using EasySep mouse CD8+ T cells isolation kit (StemCell Technologies), following manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Resting B cells were isolated using the EasySep Negative Selection- Mouse B Cell Isolation Kit (STEMCELL Technologies), which depletes for non-B cell markers and activated CD43+ B cells ...
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bioRxiv - Immunology 2022Quote: ... and incubated with NK cells isolated using the mouse NK cell isolation kit (STEMCELL Technologies cat #19855) from tumor-free wild type B6 mice in the indicated ratios in the presence of IL-2 as described previously (72) ...
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bioRxiv - Immunology 2022Quote: Bone-marrow eosinophils were isolated using EasySep™ Mouse Biotin Positive Selection Kit II (Stem Cell Technologies) according to manufacturer instructions ...
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bioRxiv - Physiology 2022Quote: Mouse jejunal section were used to isolate crypts according to the manufacturer’s instructions (STEMCELL Technologies, document 28223). Complete IntestiCult medium was exchanged every 2-3 days and organoid numbers and de novo crypts were scored on days 5 and 7 ...
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bioRxiv - Cancer Biology 2023Quote: ... All sorted cells were grown in Mouse MesenCult Expansion Kit Media (05513; StemCell Technologies, Vancouver, BC, Canada). Cells were cultured at 37 °C with 5% CO2 ...
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bioRxiv - Cancer Biology 2023Quote: ... The mouse MLL-AF9-transduced cells were cultured in MethoCult™ M3234 (STEMCELL Technologies, Bancouber, BC, Canada). The human MLL-AF9-transduced cells were cultured in StemSpan SFEM II medium (#ST-09655 ...
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bioRxiv - Cell Biology 2022Quote: ... mouse intestinal organoids were cultured in 24-well plates using IntestiCult organoid growth medium (06005, STEMCELL Technologies). In short ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were cultured for 7 d at 5% O2 in EpiCult- B mouse medium (STEMCELL Technologies, 05610) supplemented with 5% FBS ...
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bioRxiv - Immunology 2024Quote: ... Splenocytes or BM cells were enriched using EasySep™ Mouse Streptavidin RapidSpheres™ Isolation Kit (Stemcell Technologies) per the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Mouse myeloid cells were differentiated in IMDM (Cytiva, SH30228.FS) supplemented with 20% BIT9500 (StemCell Tech, 09500), 20 ng/mL M-CSF (Peprotech ...
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bioRxiv - Cell Biology 2019Quote: ... Differentiation was initiated by changing to RPMI1640 medium containing B-27 (minus insulin) and the glycogen synthase kinase-3 (GSK3) inhibitor CHIR99021 (STEMCELL Technologies, cat. 72052) for 24 h ...
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bioRxiv - Cell Biology 2022Quote: ... the tubuloids still inside the BME were washed 3 times with cold HBSS followed by a 20 min incubation with Dispase solution (StemCell Technologies, Cambridge, UK) at 37°C and 5% v/v CO2 to remove the BME around the tubuloids ...
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bioRxiv - Cancer Biology 2022Quote: ... endothelial and haematopoietic lineages in a 3-stage process as described in Figure 1A and the main manuscript text using APEL2 (StemCell Technologies Cat#05275)(61 ...
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bioRxiv - Microbiology 2021Quote: ... A nasal brushing of the turbinate was acquired using 3-mm bronchial cytology brush and placing the biopsy into warm PneumaCult-Ex Plus Medium (STEMCELL Technologies, Cambridge, UK). The cells were dissociated from the brush by gentle agitation before seeding into a single well of a collagen (PureCol from Sigma Aldrich ...
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bioRxiv - Immunology 2022Quote: ... erythrocytes were removed by treating with ACK buffer for 3 min on ice and CD4+ T cells were enriched using the EasySep™ Rat CD4+ T Cell Isolation Kit (StemCell technologies, 19642), before purification by sorting ...
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bioRxiv - Biophysics 2020Quote: ... were grown and maintained at 37°C in hypoxic conditions (3% O2, 5% CO2) in NeuroCult™ NS-A Proliferation medium (STEMCELL Technologies, 05751) supplemented with 1% Heparin Solution (STEMCELL Technologies ...
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bioRxiv - Developmental Biology 2023Quote: ... differentiating cells were confluent again and split 1:3-1:4 into Step 2 differentiation medium: BrainPhys Neuronal Medium (STEMCELL Technologies, cat# 05790), 1X N2 ...
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bioRxiv - Cell Biology 2023Quote: ... 3.3 x 105 cells and 2.6 x 105 cells per ml were seeded in 3 ml E8 medium supplemented with 10 µM Rho-associated coiled-coil kinase (ROCK) inhibitor Y-27632 (RI, STEMCELL Technologies, Vancouver, Canada), respectively ...
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bioRxiv - Neuroscience 2023Quote: ... The microglia cell differentiation through 3 steps: 1) Embryoid Body (EB) formation: using Spin-EBs formation method with AggreWellsTM800 (Stemcell Technologies, Catalog # 34825). briefly ...
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bioRxiv - Neuroscience 2023Quote: ... On day 3 cells were changed into a 50/50 ratio of fibroblast media and TeSR™ E-7™ Reprogramming Media (STEMCELL Technologies), and on day 5 were changed into 100% E-7™ Reprogramming Media ...
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bioRxiv - Neuroscience 2023Quote: ... 3 million iPSCs per cell line were plated into 1 well of an AggreWell™800 24-well plate (Stem Cell Technologies, 34811) with 2 mL STEMdiff™ Neural Induction Medium + SMADi + 10 µM Y-27632 ...
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bioRxiv - Immunology 2022Quote: ... Monocytes were isolated from bone marrow (femurs and tibias) with the EasySepTM Mouse Monocyte Isolation Kit (StemCell Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... B cells were purified from a B6 mouse using an EasySep B cell negative selection kit (StemCell Technologies) and labelled with CFSE before being transferred at 4e6 per mouse ...
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bioRxiv - Immunology 2021Quote: ... β2-/- DCs were purified from potential other cells using the EasySepTM mouse Pan-DC enrichment kit (Stemcell Technologies) and allowed to rest for 1 h before the infection assay.
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bioRxiv - Neuroscience 2020Quote: Feeder-free E14Tg2a (Baygenomics) mouse embryonic stem cells (mES) were passaged on gelatin-coated (0.1% gelatin, StemCell Technologies) cell culture treated plastic dishes using established media and cell culture techniques (Sadegh and Macklis ...
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bioRxiv - Cancer Biology 2020Quote: ... Then DC were negatively selected using the EasySep Mouse Pan-DC Enrichment Kit (Stem Cell Technologies, Cat# 19763).
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bioRxiv - Cancer Biology 2022Quote: ... C57BL/6 CD8+ T cells were purified using the EasySep Mouse CD8 T Cell Isolation Kit (Stemcell technologies) according to the manufacturer’s instructions ...