Labshake search
Citations for Stemcell Technologies :
451 - 500 of 4519 citations for Primary Human Arterial Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... B cells were purified from thawed PBMCs by negative selection using EasySep Human B Cell Enrichment Kit (Cat#19844, STEMCELL Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... Naïve CD4+ T-cells (CD3+CD4+CD45RA+CD45RO-) were purified from PBMC using the EasySep Human CD4+ T-cell Isolation Kit (Stemcell Technologies) to >95% purity according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... CD4+ T cells were enriched from blood of healthy donors using RosetteSep Human CD4+ T cell enrichment cocktail (Stemcell Technologies, Canada) according to the manufacturer’s instructions followed by Ficoll density gradient centrifugation ...
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bioRxiv - Immunology 2021Quote: ... followed by enrichment of either effector/memory T cells (Human Memory CD4 T cell Enrichment kit; EasySep) or Treg cells (Human CD4+CD127loCD49d− Treg Enrichment Kit; EasySep) (all from StemCell Technologies).
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bioRxiv - Immunology 2020Quote: ... Memory human CD8+ T cells were purified from healthy donor blood samples using the EasySep Human Memory CD8+ T cell enrichment kit (StemCell Technologies), cells were routinely ∼90% CD8+RO+RA-.
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bioRxiv - Immunology 2020Quote: Total human CD8+ T cells were purified from healthy donor blood samples using the EasySep Negative human CD8+ T cell isolation kit (StemCell Technologies). CD8+ T cells were routinely ∼90% pure ...
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bioRxiv - Immunology 2020Quote: ... Total T-cells were purified from the same donor’s PBMC using the EasySep™ Human T cell Isolation kit (StemCell Technologies). Purity of total CD4+ and CD8+ T-cells was ∼94% (Supplementary Fig 2) ...
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bioRxiv - Immunology 2022Quote: ... Bone marrow plasma cells (BMPC) were enriched from bone marrow mononuclear cells using the EasySep Human CD138 Positive Selection Kit II (StemCell Technologies) and immediately used for ELISpot or cryopreserved in 10% DMSO in FBS.
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bioRxiv - Immunology 2022Quote: ... 12 day-expanded cells were further expanded for 7 days with ImmunoCult Human CD3/CD28/CD2 T Cell Activator (25 μl/ml; STEMCELL Technologies), then rested overnight with reduced IL-2 as above (total 20-day culture) ...
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bioRxiv - Molecular Biology 2022Quote: ... density gradient centrifugation and total CD4+ T cells were enriched by negative selection with the EasySep Human CD4+ T Cell Enrichment Kit (STEMCELL Technologies) to an average purity of 90% ...
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bioRxiv - Molecular Biology 2023Quote: ... CD4 T cells were isolated from PBMCs by negative selection using EasySep Human CD4 T Cell Enrichment Kit (StemCell Technology, #19052). All participants signed informed consent forms approved by the Ethics Committee of the Ghent University Hospital (Belgium ...
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bioRxiv - Immunology 2023Quote: ... the dead cells were first removed with a Dead Cell Removal Kit and then the NK cells were depleted using the EasySep Human CD56 Positive Selection Kit (Stemcell Technologies). The control 221 cells were kept in the same culture conditions but were not exposed to NK cells ...
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bioRxiv - Cancer Biology 2023Quote: ... Bone marrow aspirates were filtered through a cell strainer and mixed with a RosetteSep human MM cell enrichment cocktail (negative selection, STEMCELL Technologies). Afterwards ...
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bioRxiv - Bioengineering 2023Quote: ... Human T cells were purified (> 96% purity) by magnetic negative selection using an EasySep Human T Cell Isolation Kit (Cat#17951, Stemcell Technologies) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2023Quote: ... CD4+ T cells were isolated from thawed PBMCs by negative magnetic selection using the EasySep Human CD4 T Cell Enrichment Kit (STEMCELL Technologies) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2023Quote: ... The percentage of CD3+ cells was determined by flow cytometry and T cells were stimulated with Immunocult Human CD3/CD28/CD2 T cell activator (StemCell Technologies) per fabricator instructions in IMDM (Gibco ...
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bioRxiv - Cell Biology 2024Quote: The CD4+ T-cell subpopulation was isolated from the CD14- fraction of PBMCs using the Easysep human CD4+ T-cell isolation kit (Stemcell Technology) as per the instructions of the manufacturer ...
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bioRxiv - Immunology 2024Quote: ... T cells were separated with negative selection magnetic beads using EasySepTM Human T Cell Isolation Kit (STEMCELL Technologies, Vancouver, BC, Canada). Second- generation 4-1BB- costimulated TSHR CAR constructs were synthesized de novo (IDT ...
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bioRxiv - Cell Biology 2023Quote: Human CD34+ cells were seeded at the concentration of 5*105 CD34+ cells/ml in serum-free StemSpan medium (StemCell Technologies) supplemented with penicillin ...
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bioRxiv - Microbiology 2024Quote: ... Naïve B cells were then isolated from adult blood using the EasySep Human Memory B Cell Isolation kit (STEMCELL Technologies #17864) to first remove CD27 positive memory cells ...
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bioRxiv - Immunology 2024Quote: ... + 1:100 Glutamax + 10 mM HEPES + 1 mM N-AcetylCystein + 1:50 B-27 Supplement + 50 ng/ml human EGF + 100 ng/ml human Noggin +100 ng/ml human IGF1 (Stemcell Technologies) + 50 ng/ml human FGF2 (Stemcell Technologies ...
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bioRxiv - Developmental Biology 2021Quote: Human ESCs were differentiated into neural progenitor cells (NPCs) using the STEMdiff neural system (STEMCELL Technologies). Briefly ...
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bioRxiv - Microbiology 2019Quote: ... or the EasySep Human CD4+ T Cell Isolation Kit (STEMCELL Technologies, Vancouver, BC, Canada, Cat. #17952) according to manufacturer’s instructions ...
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Understanding immune-driven brain aging by human brain organoid microphysiological analysis platformbioRxiv - Bioengineering 2022Quote: The human embryonic stem cell line (WA01, WiCell) was maintained with mTeSR plus medium (Stemcell Technologies) on 6 well plates coated with Matrigel (Corning) ...
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bioRxiv - Immunology 2021Quote: ... CD34+ precursors cells were isolated from PBMCs (EasySep™ Human CD34 Positive Selection Kit, STEMCELL Technologies). CD34+ cells were grown under serum-free conditions using StemSpan™ medium (STEMCELL Technologies ...
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bioRxiv - Systems Biology 2021Quote: ... Peripheral Blood Mononuclear Cells (PBMCs) were isolated using the Direct Human PBMC Isolation Kit (StemCell Technologies) according to the manufacturer’s protocol and washed/resuspended in custom RPMI-1640 deficient in biotin ...
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bioRxiv - Cancer Biology 2020Quote: ... Red blood cell lysis and leukocyte depletion (EasySep Human CD45 Depletion Kit II #17898, STEMCELL Technologies) of MPE were performed before processing with 10X Genomics ...
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bioRxiv - Immunology 2021Quote: ... plasma cells were isolated using the EasySep Human CD138 Positive Selection Kit II (Stemcell Technologies, #17877) for analysis in a companion study (manuscript in preparation) ...
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bioRxiv - Bioengineering 2021Quote: ... 25 µL/mL of ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator (Stemcell Technologies - #10970) were used ...
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bioRxiv - Immunology 2019Quote: ... Human Cord Blood (CB) purified CD34+ cells from 5 different donors were obtained from STEMCELL Technologies and resuspended in StemSpan Expansion Media – SFEM II (STEMCELL Technologies ...
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bioRxiv - Immunology 2020Quote: ... cells in full media were incubated with Human Recombinant IFNα-2A (10ng/mL, StemCell Technologies 78076.1) or IFNγ (10ng/mL ...
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bioRxiv - Immunology 2019Quote: Human T-cell subsets were isolated from leukapheresis produts using negative selection kits from STEMCELL Technologies. Rosette Sep (Stemcell Technologies ...
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bioRxiv - Immunology 2021Quote: ... CD34+ precursors cells were isolated from PBMCs (EasySep™ Human CD34 Positive Selection Kit, STEMCELL Technologies). CD34+ cells were grown under serum-free conditions using StemSpanTM medium (STEMCELL Technologies ...
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bioRxiv - Immunology 2021Quote: ... negative selection was performed using EasySep Human T cell isolation kit (#17951, Stemcell technologies, Vancouver, Canada). The CD3+ T cells were labelled with 1µM Cell Trace Violet dye (#C34557 ...
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bioRxiv - Immunology 2022Quote: ... and CD8+ T cells were subsequently isolated using the Stemcell human CD8+ isolation kit (Stemcell Technology) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... NGFR+ cells were bead-purified by an EasySep Human CD271 Positive Selection Kit II (STEMCELL Technologies). See Fig ...
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bioRxiv - Immunology 2022Quote: Frozen human bone marrow mononuclear cells (BMMC) from three healthy donors were purchased from STEMCELL Technologies and stored in liquid nitrogen until use ...
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bioRxiv - Developmental Biology 2023Quote: ... to collect mononuclear cells then purified using EasySep Human Cord Blood CD34 Selection (StemCell Technologies, #17896). HUCB CD34+ cells were then seeded in Matrigel Matrix (Cat# 356231 ...
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bioRxiv - Cell Biology 2023Quote: Human induced pluripotent stem cells (iPSCs) were maintained on vitronectin XFTM (10 μg/mL, StemCell Technologies)-coated plates and in Essential 8 (E8 ...
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bioRxiv - Cancer Biology 2023Quote: ... 5g were isolated from human peripheral blood mononuclear cells (PBMCs) (StemCell Technologies, 70025, individual donors selected) using EasySepTM Human CD11b Positive Selection and Depletion Kit (StemCell Technologies ...
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bioRxiv - Genomics 2023Quote: ... CD14+ monocytes were purified using EasySep Human Monocytes Isolation Kit (negative selection kit; Stem cell Technologies) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: Human embryonic stem cells (hESCs; H1) were maintained in mTeSR1 plus media (StemCell Technologies, 100-0276) on Matrigel (Corning ...
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bioRxiv - Bioengineering 2024Quote: ... Tregs were isolated using the EasySep human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit (StemCell Tech.) and cultured in X-Vivo15 ...
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bioRxiv - Cancer Biology 2024Quote: ... in EpiCult™-B (mouse) medium supplemented with human recombinant EGF (hrEGF) (Stem Cell Technologies, 78006), human recombinant bFGF (hrbFGF ...
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bioRxiv - Cancer Biology 2020Quote: Primary FL cell-suspensions were defrosted at 37°C and layered onto 3ml of lymphoprep (STEMcell Technologies). Lymphocytes were isolated by centrifugation at 1150g for 12 minutes ...
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bioRxiv - Microbiology 2019Quote: ... CD4+ T cells at 106 cells/mL were stimulated with 25 μL/mL ImmunoCult™ Human CD3/CD28 T Cell Activator (STEMCELL Technologies, Vancouver, Canada, catalogue #10991). At day 3 post-stimulation ...
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bioRxiv - Cancer Biology 2021Quote: ... Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation and CD34+ cells were separated using EasySep human CD34+ selection cocktail (StemCell Technologies, Inc.). CD34+ cells were short-term cultured in IMDM medium (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... CD4+ or CD8+ T-Cells were isolated from PBMC using EasySep™ Human CD4+ or CD8+ T-Cell Isolation Kit (STEMCELL Technologies) according to the manufacturer’s protocols ...
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bioRxiv - Immunology 2021Quote: Primary NK cells were isolated from peripheral blood lymphocytes (PBLs) of healthy donors using the EasySep™ human NK Cell enrichment kit (STEMCELL Technologies). Subsequently ...
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bioRxiv - Microbiology 2021Quote: ... cells were centrifuged at 500 x g for 15 min at room temperature and were resuspended in RoboSep buffer for B cells isolation by negative selection with EasySep™ Human B Cell Isolation Kit (Stemcell Technologies) according to manufacturer’s instructions ...