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Citations for Stemcell Technologies :
451 - 499 of 499 citations for IL 10 Rat since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... EBs were embedded in Matrigel and transferred to 10-cm cell-culture dishes coated with anti-adherence rinsing solution (Stemcell technologies, P2443) in 15ml NI ...
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bioRxiv - Bioengineering 2023Quote: ... Single cells were centrifuged at 200g for 5 minutes and resuspended in StemFlex supplemented with 10 µM Y-27632 (StemCell Technologies Inc.). We seeded 5.5×106 cells per 5.5 mL of mTeSR+ in each well of a six-well suspension plate ...
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bioRxiv - Cancer Biology 2023Quote: ... Matrigel was allowed to polymerize at 37 °C for 10-15 minutes before mouse IntestiCult Organoid Growth Medium (STEMCELL Technologies cat#06005), containing Y-27632 (10 μM) ...
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Prevention of tau accumulation through inhibition of hnRNP R-dependent axonal Mapt mRNA localizationbioRxiv - Neuroscience 2023Quote: ... Alomone Labs), 10 ng/mL brain-derived neurotrophic factor (167450-02-STD, tebu-bio) and 500 µM dibutyryl-cAMP (Stemcell Technologies, 73886) and motoneurons were maintained for 25 DIV.
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bioRxiv - Genetics 2024Quote: ... cells were harvested by centrifugation at 380 x g for 10 min at 4°C and resuspended in EasySepT Buffer (Stem Cell Technologies). HSPCs were purified by negative selection using the EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (Stem Cell Technologies) ...
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bioRxiv - Neuroscience 2024Quote: ... Embryoid body media (EBM) was used for days 2 to 11 (Supplementary Table 2) and supplemented with 10 µM SB431542 (Stemcell Technologies, #72234), 0.1 µM LDN193189 (MedChemExpress ...
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bioRxiv - Cancer Biology 2024Quote: MDA-MB-468 and mitomycin-C treated NIH-3T3 cells were plated at a ratio of 10:1 in AggreWellTM400 plates at approximately 50 cells/microwell according to the manufacturer’s instructions (#34415, Stemcell Technologies, Vancouver, BC) and grown for 5 days in tumor media containing 1μg/mL doxycycline ...
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bioRxiv - Cell Biology 2021Quote: ... A total number of 3.75 × 105 ThF iPSCs were plated in a T25 flask in the mTeSR1 medium with ROCK Inhibitor Y-27632 (10 µM, Stem cell Technologies, #72304). After 24 hours ...
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bioRxiv - Bioengineering 2020Quote: ... and plated onto Matrigel®-coated six-well culture plates in mTeSR™1 supplemented with 10 μM Rho-associated protein kinase inhibitor Y27632 (ROCK inhibitor; Stem Cell Technologies) at a density between 25,000 and 50,000 cells cm-2 ...
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bioRxiv - Neuroscience 2021Quote: ... cells were plated on Matrigel (In Vitro Technologies, cat. # FAL354277) coated plates in StemFlex medium supplemented with 10 μM ROCK inhibitor (StemCell Technologies, cat. #. 72304). Three days after plating ...
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bioRxiv - Cell Biology 2021Quote: ... YTS cells were cultured in RPMI media supplemented with 10% fetal bovine serum and NK92 cells were cultured in Myelocult (Stemcell Technologies, catalogue H5100) supplemented with 200 U/ml of IL-2 (Roche ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were resuspended in PBS+2% FBS and then processed for FACS staining or frozen in 10% Cryostor (Stem Cell Technologies, Vancouver, BC). For intracellular staining ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were resuspended in PBS+2% FBS and then processed for FACS staining or frozen in 10% Cryostor (Stem Cell Technologies, Vancouver, BC).
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bioRxiv - Developmental Biology 2022Quote: ... and 105 cell/well were plated as small colonies (10-20 cell/colony) on Vitronectin-coated 12-well plates using mTeSR1 media (STEMCELL Technologies, Catalog# 12604013) supplemented with 10 μM ROCK inhibitor (Y-27632 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were cultured in serum-free conditions in NeuroCult NS-A Proliferation solution with 10% proliferation supplement (STEMCELL Technologies; Cat# 05750 and #05753) supplemented with 10 ng/ml basic fibroblast growth factor and 20 ng/ml human epidermal growth factor.
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bioRxiv - Cancer Biology 2022Quote: H1 human ESCs were dissociated using accutase and plated as single cells in mTeSR™1 medium supplemented with ROCK inhibitor Y-27632 (10 μM, StemCell Technologies, Inc.) on matrigel to a final density of 20,000-30,000 cells per cm2 ...
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bioRxiv - Cancer Biology 2020Quote: ... The epidermis was separated from the dermis by placing the strips in tubes containing 10 ml of 5U/ml Dispase II (Stem Cell Technologies, USA) and incubated at 4°C overnight and at 37°C for 2-3 hours ...
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bioRxiv - Developmental Biology 2022Quote: ... and 20 ng/ml BMP4 supplemented with 10 μM Y-27632 in each well of 6-well AggreWell 400 plates (Stem Cell Technology, 34415). Next day the formed cell spheroids clusters were washed with RPMI-1640 medium with 1x glutamax ...
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bioRxiv - Developmental Biology 2022Quote: ... NPC-iPS cells were treated with 10 µM Rho kinase inhibitor Y-27632 overnight before dissociated to single cells with Accutase (Stem Cell Technology 07922). About 10 million cells were electroporated with 10 μg of AAVS1 zinc finger nuclease-encoding plasmid and 30 μg of AAVS1-tdTomato with one electroporation of 250 V ...
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bioRxiv - Microbiology 2019Quote: CD34+ HPCs isolated from human bone marrow were infected at an MOI of 2 for 20h in IMDM supplemented with 10% BIT9500 serum substitute (Stem Cell Technologies, Canada), 2 mM L-Glutamine ...
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bioRxiv - Cell Biology 2021Quote: ... to disperse into single cells and replated in mTeSR1 supplemented with 1% P/S containing 10 µM Rock Inhibitor (Y-27632, Stem Cell Technologies #72308). A full protocol is available at the Allen Cell Explorer (www.allencell.org/methods-for-cells-in-the-lab SOP ...
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bioRxiv - Cancer Biology 2021Quote: Metastatic PDX tissues that had been frozen down in either FBS containing 10% dimethyl sulfoxide (DMSO) or primary PDX tissues that were frozen in neuronal stem cell medium (NSC medium, Stemcell technologies, Köln, Germany) containing 10% DMSO were thawed by brief incubation at 37°C and were transferred to basal NSC medium ...
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bioRxiv - Bioengineering 2022Quote: ... until a final volume of 200 ml and layered per 25 ml on top of 10 ml LymphoprepTM (07851, StemCell technologies, Köln, Germany) in 50 ml centrifugal tubes ...
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bioRxiv - Developmental Biology 2022Quote: ... and 1 × 106 cells were seeded per well of an Aggrewell™800 plate in APEL2 medium supplemented with 10 μM Y-27632 ROCK inhibitor (#72307, StemCell Technologies Inc.) followed by 72h incubation with daily media change ...
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bioRxiv - Cell Biology 2023Quote: ... buffy coat-PBS-citrate solution was added in portions of 25 ml to tubes filled with 10 ml LymphoprepTM (07851, StemCell technologies, Köln, Germany). Tubes were centrifuged (20 min at RT ...
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bioRxiv - Cell Biology 2023Quote: ... 3.3 x 105 cells and 2.6 x 105 cells per ml were seeded in 3 ml E8 medium supplemented with 10 µM Rho-associated coiled-coil kinase (ROCK) inhibitor Y-27632 (RI, STEMCELL Technologies, Vancouver, Canada), respectively ...
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bioRxiv - Neuroscience 2023Quote: ... cells were fed with 10 ng/mL of neurotrophic factors (BDNF, GDNF, IGF-1 from R&D systems, NT3 from Stem Cell Technologies), ascorbic acid (2.27 µM) ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 2% (v/v) B27™ minus insulin and 1% (v/v) GlutaMax™) supplemented with SB431542 (10 µM) and Dorsomorphin (1 µM, both STEMCELL Technologies). Medium was replaced daily ...
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bioRxiv - Cancer Biology 2023Quote: ... Pellets were then suspended with 1 ml pre-warmed to 37°C A-mTE-D2 (7 mg ml-1 Dispase II, Worthington NPRO2, and 10 µg ml-1 Deoxyribonuclease I, Stem Cell Technologies, 07900), and mixed 70 times with a 1000 µl pipette tip ...
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bioRxiv - Immunology 2024Quote: ... PBMCs were isolated from whole blood using density gradient centrifugation at 1,200 x g for 10 min where diluted blood was layered over 15 mL of Lymphoprep™ (STEMCELL Technologies, Vancouver, Canada) followed by harvesting of the buffy coat ...
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bioRxiv - Neuroscience 2024Quote: ... media was replaced every other day with neuronal differentiation media prepared based on the Stem Cell Technology protocol: 10 ml of the BrainPhys™ Neuronal Medium (#05790; Stem Cell Technology) supplemented with 200 µl of NeuroCultTM SM1 Neuronal Supplement (#05711 ...
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bioRxiv - Cancer Biology 2024Quote: ... Corning 10-092-CV; 20% fetal bovine serum [FBS], Sigma-Aldrich F4135; and 0.1 mg ml-1 DNase I, Stem Cell Technologies 07900). Cell suspension was placed in a new tube ...
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bioRxiv - Synthetic Biology 2021Quote: ... in borate buffer for primary culture cells or Poly-D-Lysine for cells from an iPSC background before being coated with either 10 µg/ml mouse laminin or 5 µg/ml human 521 Laminin (Stemcell Technologies Australia, Melbourne, Australia) respectively to facilitate cell adhesion.
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bioRxiv - Bioengineering 2022Quote: ... until a final volume of 200 ml and layered per 25 ml on top of 10 ml Lymphoprep™ (07851, StemCell technologies, Köln, Germany) in 50 ml centrifugal tubes ...
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bioRxiv - Neuroscience 2021Quote: ... and plated at 3 × 106 cells/well to allow the embryoid body (EB) formation in Neural Induction Media (NIM) + 10 μM Y-27632 (Stem Cell Technologies, Cat.No. 72308). They were allowed to attach for at least overnight ...
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bioRxiv - Immunology 2022Quote: ... until a final volume of 200 ml and layered per 25 ml on top of 10 ml Lymphoprep™ (07851, StemCell technologies, Köln, Germany) in 50 ml centrifugal tubes ...
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bioRxiv - Cancer Biology 2023Quote: ... Isolated T cells were seeded at a density of 1 x 106 cells/mL in RPMI medium supplemented with 10 ng/mL human recombinant interleukin 2 (IL2, Stemcell Technologies, Cat. No. 78145.2), 1µg/ml anti-human CD3 antibody (BioLegend ...
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bioRxiv - Cell Biology 2022Quote: ... the dissociated cells were resuspended in the transduction medium (1 uL to 5 uL of lentivirus, 4 to 8 ug/ml Polybrane[STR-1003-G, Sigma Aldrich], and 10 uM Y27632 [72304, StemCell Technologies] in mTeSR1), and centrifuged at 3200 xg for 30 to 90 min ...
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bioRxiv - Genomics 2022Quote: CD4 T-cells were isolated from 10-20 ml of peripheral blood using EasySep Direct Human CD4+ T cell Isolation kit (Stem Cell Technologies, cat #19662). The isolated CD4 T-cells were stored in Crystor CS10 (Stem Cell Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... were treated with drugs at a final concentration of 10 µM and mixed with MethoCult™ H4100 Base medium (Stem cell technologies, Canada, 04100) enriched with 20% FBS ...
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bioRxiv - Bioengineering 2023Quote: The hPSCs were cultured in NuwacellTM ncTarget hPSC Medium (Nuwacell Biotechnologies, RP01020, Hefei, China) with 10 μM Y-27632 (STEMCELL Technologies, 72304, BC, USA). The hNSCs were derived from hiPSCs which followed standard neurobiology protocols (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2024Quote: ... and the organoid pellet was resuspended in 5-10 mL pre-cooled basal DMEM/F-12 medium containing 15 mM HEPES (Stem Cell Technologies, cat.# 36254) using a 10 mL pipette pre-wetted with 1% (vol/vol ...
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bioRxiv - Cancer Biology 2021Quote: ... L0 and S7 cells were grown as floating spheres and maintained in NeuroCult NS-A Proliferation medium and 10% proliferation supplement (STEMCELL Technologies; Cat# 05750 and #05753), 1% penicillin–streptomycin (Thermofisher ...
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bioRxiv - Immunology 2021Quote: ... T cells from were then activated with Miltenyi MACS GMP T cell TransAct™ CD3/CD28 beads and seeded 1×10^6 T cells/ml in serum-free StemCell Immunocult™-XF media (StemCell Technologies, Vancouver, Canada) with clinical grade 20U/ml human IL-2 (Novartis)
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bioRxiv - Cancer Biology 2022Quote: ... L0 and S7 cells were grown as floating spheres and maintained in NeuroCult NS-A Proliferation medium and 10% proliferation supplement (STEMCELL Technologies; Cat# 05750 and #05753), 1% penicillin–streptomycin (Thermofisher ...
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bioRxiv - Cancer Biology 2022Quote: ... using base medium (1:1 ratio of DMEM/F12 Glutamax/Neurobasal medium [Gibco], 2% B27 supplement without vitamin A [Gibco], 10% BIT 9500 [StemCell Technologies], and 1 mM glutamine [Gibco]) supplemented with 20ng/ml EGF (Chemicon) ...
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bioRxiv - Cell Biology 2022Quote: WIBR3 (NIHhESC-10-0079) hESCs and isogenic LIS1+/- 10 were grown and maintained on irradiated MEFs in optimal naive NHSM conditions (RSET-Stem Cell Technologies INC, supplementary Table 1) 90 ...
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bioRxiv - Physiology 2020Quote: ... were then cultured in the complete L15 medium or the stem cell (SC) medium (10% human MammoCult Proliferation Supplements in MammoCult Basal Medium, Stem Cell Technologies, San Diego, CA, USA) for 7 days ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 3 units/ml of human EPO (Peprotec, #100-64) and 10 ng/ml of G-CSF (Filgrastim, Millipore Sigma, 121181-53-1) (Stem Cell Technologies, Vancouver, BC, Canada).