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Citations for Stemcell Technologies :
451 - 500 of 2126 citations for Human Procollagen Type III N Terminal Propeptide PIIINP ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: HSPCs were purified from discarded umbilical cord blood samples of healthy male or female newborns using the EasySep Human CD34 Positive Selection Kit II following pre-enrichment using the RosetteSep Pre-enrichment cocktail (Stem Cell Technologies) and mononuclear cell isolation on Ficoll-Paque (GE Healthcare ...
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bioRxiv - Immunology 2021Quote: ... a second bead-based purification step was included according to the manufacturer’s instructions (EasySepTM Human Isolation Kit from STEMCell Technologies, Cologne, Germany). While density centrifugation led to 90% pure PMN ...
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bioRxiv - Immunology 2021Quote: ... The negative fraction of the above selections was divided into two aliquots that were subjected to negative immunomagnetic isolation of either B cells (EasySep Human Pan-B cell Enrichment Kit, Stemcell Technologies, #19554) or T cells (EasySep Human T cell Isolation Kit ...
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bioRxiv - Cancer Biology 2021Quote: ... Isolation of B-cells from healthy donor and MM patients were performed using EasySepTM Human B-Cell Isolation Kit according to manufacturer’s protocol (Cat. No. 17954, Stemcell Technologies, Vancouver, Canada).
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bioRxiv - Cancer Biology 2021Quote: CD138+ myeloma cells were isolated from patient peripheral blood using a magnetic bead-based approach (EasySep Human CD138 Positive Selection Kit, StemCell Technologies 17877). The CD138+-enriched sample was titrated into 2.5e7 ...
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bioRxiv - Cancer Biology 2022Quote: ... mobilized peripheral blood mononuclear cells (PBMCs) and cord blood) using magnetic beads according to manufacturer’s protocol (EasySepTM Human CD3 Positive Selection Kit II, StemCell Technologies, Vancouver, Canada)
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bioRxiv - Biochemistry 2021Quote: ... CD138+ primary myeloma cells were purified from fresh bone marrow aspirates of multiple myeloma patients using EasySep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit II (StemCell Technologies) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: CD4 T cells were isolated from PBMC by negative magnetic selection using the EasySep Human CD4 T Cell Enrichment Kit (StemCell Technology, 19052). Purity was typically >98%.
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bioRxiv - Immunology 2020Quote: ... The negative fraction of the above selections was divided into two aliquots that were subjected to negative immunomagnetic isolation of either B cells (EasySep Human Pan-B cell Enrichment Kit, Stemcell Technologies, #19554) or T cells (EasySep Human T cell Isolation Kit ...
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bioRxiv - Immunology 2020Quote: ... Primary CD4+ T cells were isolated from peripheral blood mononuclear cells (PBMCs) and enriched via negative selection with an EasySep Human CD4+ T Cell Isolation Kit (Stemcell Technologies, Canada). Primary CD4+ T cells were cultured at 37°C in RPMI supplemented with 10% FBS ...
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bioRxiv - Immunology 2021Quote: 1 × 105 memory CD4+ T cells freshly purified from PBMC by negative selection and magnetic separetion(EasySep™ Human memory CD4+ T cell enrichment kit, STEMCELL Technologies) were cocultured at 1:1 ratio for 6 days with anti-CD3/CD28–coated beads (Dynabeads ...
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bioRxiv - Immunology 2021Quote: ... Treg were purified from CD4+ memory T cells by magnetic separation (EasySep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit, STEMCELL Technologies) and labelled with CellTrace™ Violet ...
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bioRxiv - Immunology 2020Quote: ... And pNK cells were isolated from whole blood by negative selection using the EasySep™ Direct Human NK cell Isolation Kit (StemCell Technologies). Following isolation ...
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bioRxiv - Immunology 2021Quote: ... density-gradient centrifugation and CD34+ cells were isolated using anti-CD34 immunomagnetic beads (positive selection) following the manufacturer’s instructions (EasySepTM Human cord blood CD34+ positive selection kit II, STEMCELL Technologies Cat#17896). CD34+ HSPCs were either freshly used or cryo-preserved until future use ...
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bioRxiv - Molecular Biology 2023Quote: ... T cells were subsequently isolated from the PBMC population by immunomagnetic negative selective with the EasySep Human T Cell Isolation kit (StemCell Technologies, Canada). Isolated T cells with >95% viability and >95% purity were either cryopreserved or directly cultured for subsequent experiments ...
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bioRxiv - Cancer Biology 2023Quote: ... After an additional 48 hours non-adhered cells were discarded and adhered T-ALL cells were separated from glioblastoma cells using EasySep Human CD3 positive Selection kit II (Stem Cell Technologies) and viability was determined by trypan blue staining (Bio-Rad) ...
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bioRxiv - Immunology 2024Quote: For further examination of neutrophil granulocytes we isolated granulocytes with the EasySep® Direct Human Neutrophil Isolation Kit (Stemcell Technologies, Cat# 19666), following the instructions provided by the manufacturer ...
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bioRxiv - Cancer Biology 2023Quote: ... Naïve CD8+ T-cells were isolated from the thawed CD14-population on Day 6 using the EasySep Human Naïve CD8+ T Cell Isolation Kit (STEMCELL Technologies Cat. #19258) as per manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... NK cells were isolated from whole blood using a density based negative selection kit according to the manufacturer’s instructions (RosetteSep Human NK Enrichment Cocktail, STEMCELL Technologies, Vancouver, Canada). Isolated PB-NK cells were expanded with irradiated K562-mb15-41BBL feeder cells (1-3 days before NK cell isolation ...
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bioRxiv - Bioengineering 2024Quote: ... CD56+CD3- NK cells were isolated from the PBMC population using the EasySep Human NK Cell Isolation Kit (STEMCELL Technologies, Cambridge, MA). NK cells were frozen at 2.5e6 or 5E6 cells/mL in CryoStor CS10 (STEMCELL Technologies ...
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bioRxiv - Immunology 2022Quote: ... Conditionned NSG mice were humanized with CD34+ hematopoietic stem cells isolated from human cord blood (CB) using a magnetic activated cell sorting CD34+ progenitor cell isolation kit (Stem Cell Technologies). CB was provided by the Cord Blood Bank Central Hospital University (Liège ...
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bioRxiv - Microbiology 2022Quote: Memory CD4+ T cells were enriched by negative selection using the EasySep Human Memory CD4+ T Cell Enrichment Kit (STEMCELL Technologies #19157) following the recommended protocol ...
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bioRxiv - Bioengineering 2022Quote: We isolated B cells from healthy donors’ peripheral blood mononuclear cells (Fred Hutchinson Cancer Research Center) using the EasySep Human B cell isolation kit (Stem Cell Technologies). Isolated B cells were cultured in Iscove’s modified Dulbecco’s medium (Gibco ...
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bioRxiv - Immunology 2023Quote: ... CD14+CD16-monocytes were isolated from peripheral blood mononuclear cells using immunomagnetic negative selection (EasySep Human Monocyte Isolation Kit, Stem Cell Technologies).
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bioRxiv - Bioengineering 2024Quote: ... Neutrophils were retained in solution through negative immuno-magnetic separation through the EasySep™ Human Neutrophil Isolation Kit (STEMCELL Technologies, Vancouver, Canada). Neutrophils were incubated in Iscove’s Modified Dulbecco’s Medium (IMDM ...
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bioRxiv - Immunology 2024Quote: ... PBMCs were thawed and CD4+ T cells were isolated by immunomagnetic negative selection (EasySep™ Human CD4+ T Cell Enrichment Kit, StemCell Technologies). 250,000-500,000 CD4+ T cells per well were plated in 96-well V-bottom plates ...
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bioRxiv - Immunology 2024Quote: Primary monocytes were isolated via negative selection using the EasySep™ Human Monocyte Isolation Kit (Easy Sep, 1x10^9) (Stem Cell Technologies) according to the manufacturer’s protocols from PBMCs collected from healthy donors ...
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bioRxiv - Immunology 2024Quote: ... CD3+ Tresponder cells (previously enriched from an HLA-A3+ individual using the EasySep™ Human CD3 Positive Selection Kit II; STEMCELL Technologies) were labelled with CPD-eFluor™450 and added to the co-cultures at a 5:1 Tresponder:DC ratio ...
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bioRxiv - Immunology 2024Quote: B cells were isolated from 2 healthy PBMC donors using the EasySep™ Human B-cell Isolation Kit (Catalog #17954) from StemCell Technologies. Cells were then stained with APC anti-IgD-APC ...
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bioRxiv - Immunology 2024Quote: ... cones obtained from the Oklahoma Blood Institute (Oklahoma City, OK) using negative selection with the EasySep™ Human T Cell Isolation Kit (cat. no. 17951; STEMCELL Technologies). Alternatively ...
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bioRxiv - Immunology 2024Quote: ... 10 x 106 PBMCs were enriched for B cells using CD3 T cell depletion via the EasySep™ Human CD3 Positive Selection Kit II (StemCell Technologies) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2024Quote: ... the mice were irradiated (350 rads) and injected intravenously with CD3+ T cell-depleted cord blood (EasySep Human CD3 Positive Selection Kit, 18051, Stem Cell Technologies) containing approximately 105 human CD34+ hematopoietic stem cells (HSC ...
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bioRxiv - Immunology 2024Quote: ... B-cells were then purified from the other harvested PBMCs by negative selection using a Human B-cell Isolation Kit (Stem Cell Technologies) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Mario-CAR T cells were selected using the EasySep™ Human PE Positive Selection Kit II (Stem Cell Technologies, Cat-no. 17654) with PE NGFR antibody (Biolegend ...
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bioRxiv - Neuroscience 2024Quote: ... B cells were then enriched by negative selection using EasySep™ Human B Cell Enrichment Kit II Without CD43 Depletion (17963, Stemcell Technologies) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Neural Precursor Cells (NSCs) were generated from human induced pluripotent stem cells (iPSCs) using STEMdiff™ SMADi Neural Induction kit (STEMCELL Technologies) via dual SMAD inhibition as per the manufacturers protocol ...
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bioRxiv - Cancer Biology 2024Quote: Primary human NK cells were isolated from PBMCs from three donors by negative selection using the EasySepTM Human NK Cell Isolation Kit (STEMCELL Technologies 17955), according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: PBMCs were thawed and CD4+ T cells were isolated by immunomagnetic negative selection (EasySep™ Human CD4+ T Cell Enrichment Kit, StemCell Technologies). 100,000-200,000 CD4+ T cells per well were plated in 96-well V-bottom plates and rested for 1.5 hours at 37 °C ...
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bioRxiv - Neuroscience 2024Quote: ... Effector natural killer (NK) cells were isolated from human peripheral blood using Rosette Sep NK isolation kit (Stem Cell Technologies, Vancouver, Canada). NK cells were stimulated overnight with IL-21 (20 ng/mL ...
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bioRxiv - Biochemistry 2024Quote: ... Monocytes were purified from the PBMC fraction using the EasySep™ Human CD14 Positive Selection Kit II (# 17858, STEMCELL Technologies, Cologne, Germany) according to the manufacturer’s instructions and seeded in 6-well cell culture plates (1.5 x 106 cells/well ...
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bioRxiv - Immunology 2024Quote: B cells from tonsils of 5 individuals were enriched using negative selection magnetic beads (EasySep Human B Cell Isolation kit, Stemcell technologies, #17954). They were then pooled to avoid batch effects ...
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bioRxiv - Cell Biology 2020Quote: ... cells were dissociated in collagenase type IV followed by Accutase (Stemcell Technologies, BC, Canada) and the fragmented cells were passaged to a new collagen-coated plate.
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bioRxiv - Immunology 2024Quote: ... 3 ml of prewarmed collagenase type IV (1mg/mL) digestion medium (StemCell Tech 07909) was added to cell pellet ...
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bioRxiv - Synthetic Biology 2020Quote: Human natural killer cells (Stemcell Technologies, Canada) were incubated with 5×105 skin fibroblasts for 60 min at 37°C ...
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bioRxiv - Developmental Biology 2020Quote: ... and human (MethoCult™ H4434, Stemcell Technologies) differentiating PSC ...
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bioRxiv - Cell Biology 2023Quote: Human PBMCs were purchased from STEMCELL Technologies and Precision for Medicine ...
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bioRxiv - Cancer Biology 2023Quote: ... human CD8+ T cells (StemCell Technologies, 70027), CD4+ T cells ...
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bioRxiv - Immunology 2024Quote: ... + 50 ng/ml human FGF2 (Stemcell Technologies) + 500 nM A-8301 (Selleckchem ...
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bioRxiv - Immunology 2024Quote: ... + 25 ng/ml human FGF10 (Stemcell Technologies) + 25 ng/ml human Noggin (Stemcell Technologies ...
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bioRxiv - Immunology 2024Quote: ... + 25 ng/ml human Noggin (Stemcell Technologies) + 10 vol% conditioned medium containing Rspondin1 (produced from HA-R-Spondin1-Fc HEK293T cell line ...