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Citations for Stemcell Technologies :
451 - 500 of 1147 citations for Human PDGF alpha receptor PDGFRA qPCR Primer Pair since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... Human PBMCs were isolated by density centrifugation using Lymphoprep (07801, StemCell Technologies, Vancouver, Canada). Some of the isolated PBMCs were subsequently cryopreserved in a solution of 90% FBS and 10% DMSO for PBMC recruitment assay ...
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bioRxiv - Cell Biology 2024Quote: ... Monocytes were enriched using the RosetteSep Human Monocyte Enrichment Cocktail (StemCell Technologies, Cambridge, UK) and separated on a Ficoll-Paque gradient (VWR ...
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bioRxiv - Cell Biology 2024Quote: ... using Neurocult basal media supplemented with human Neurocult proliferation supplement (#05751, Stem Cell Technologies), both at 9:1 ratio ...
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bioRxiv - Cancer Biology 2024Quote: ... T cells were isolated using the EasySep Human T cell isolation kit (StemCell Tech. ...
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bioRxiv - Cancer Biology 2024Quote: ... TILs were isolated with the EasySep Human CD3 Positive Selection Kit II (StemCell Tech.). Following initial activation with anti-CD3/CD28 dynabeads (Thermo Fisher Sci.) ...
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bioRxiv - Cell Biology 2024Quote: Human iPSCs were cultured on Matrigel-coated plates in mTeSR™ medium (STEMCELL Technologies) and directly differentiated into induced neurons (iNs ...
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bioRxiv - Cancer Biology 2024Quote: ... Human Peripheral blood mononuclear cells (PBMCs) were obtained from Stemcell Technologies (Vancouver, BC, Canada) and cultured in KCLB medium ...
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bioRxiv - Cell Biology 2024Quote: ... 50 ng/mL Human Recombinant brain-derived neurotrophic factor (BDNF) (Stem Cell Technologies, 78005), 0.2 mM dibutyryl cyclic AMP (db-cAMP ...
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bioRxiv - Genetics 2023Quote: ... Common karyotypic abnormalities were measured using the qPCR-based hPSC Genetic Analysis Kit following manufacturer’s instructions (STEMCELL Technologies; cat. no. 07550). Cell lines with true karyotypic abnormalities were not used in this study.
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bioRxiv - Neuroscience 2023Quote: ... The genetic integrity of the engineered hiPSC clones was evaluated by a qPCR-based hiPSC genetic analysis kit (Stem Cell Technologies).
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bioRxiv - Molecular Biology 2021Quote: ... and activated with Immunocult™ human CD3/CD28 T cell activator (Stemcell Technologies, Vancouver, Canada), in the RPMI-1640 media supplemented with 2mM L-Glutamine ...
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bioRxiv - Cancer Biology 2020Quote: BT088 and BT054 cells9 were grown in Human Neurocult proliferation media (Stem Cell Tech; # 05751) containing human epidermal growth factor (hEGF ...
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bioRxiv - Bioengineering 2020Quote: ... CD8+ cells were isolated using the RosetteSep Human CD8+ T Cell Enrichment Cocktail (StemCell Technologies) following manufacturer’s protocols ...
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bioRxiv - Immunology 2021Quote: ... we respectively used mouse- and human-specific T cell negative isolation MACS (STEMCELL Technologies, Canada). We plated 0.5–1 × 106 T cells per well in 96-well V-bottom tissue culture plates ...
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bioRxiv - Bioengineering 2020Quote: Human T cells were obtained by purchasing whole blood units from STEMCELL technologies (Vancouver, Canada). STEMCELL reported that these products are distributed using Institutional Review Board (IRB)-approved consent forms and protocols ...
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bioRxiv - Bioengineering 2020Quote: ... CD8+ T cells were isolated using RosetteSep Human CD8+ T Cell Enrichment Cocktail (StemCell Technologies) following manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... Naïve primary human T cells (CD3+/CD4+/CD45RA+) were also sourced from STEMCELL Technologies (#70029) and cultured in RPMI with 10% FBS and IL-2 as described above ...
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bioRxiv - Bioengineering 2022Quote: ... T cells were collected using negative selection (Stemcell Technologies EasySep Human T Cell Isolation Kit) from autologous healthy donor PBMCs ...
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bioRxiv - Immunology 2022Quote: ... and human B-cell activating factor (BAFF) in 80 mL StemCell medium A (StemCell Technologies) containing 1 μg/mL of cyclosporine A (Millipore-Sigma) ...
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bioRxiv - Developmental Biology 2022Quote: ... gradient centrifugation using the RosetteSep Human NK Cell Enrichment Cocktail (STEMCELL Technologies, Vancouver, BC, Canada).
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bioRxiv - Cell Biology 2021Quote: Human cord blood CD34+ hematopoietic stem and progenitor cells (HSPCs) were purchased (StemCell Technologies #78003) or acquired through the Binns Program for Cord Blood Research at Stanford University under informed consent ...
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bioRxiv - Cancer Biology 2021Quote: Human Peripheral Blood Pan-T Cells was purchased from Stem Cell Technologies (Vancouver, BC, Canada) and Panc-1 cells were obtained from the Kansas University Medical Center ...
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bioRxiv - Genetics 2022Quote: ... CD34+ HSPCs were enriched by EasySep Human Cord Blood CD34+ positive selection kit (StemCell Technologies). Enriched cells were nucleofected by AAVS1 control and MYSM1 sgRNAs ...
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bioRxiv - Immunology 2022Quote: ... NK cells were isolated using the EasySep Human NK Cell Isolation Kit (Stem Cell Technologies) following manufacturer instructions ...
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bioRxiv - Immunology 2022Quote: ... Monocytes were isolated from PBMCs using an EasySep Human Monocyte Isolation Kit (all STEMCELL Technologies).
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bioRxiv - Immunology 2020Quote: ... or after NK cell enrichment using the EasySep Human NK Cell Enrichment Kit (STEMCELL Technologies). To measure antigen-specific NKCL responses ...
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bioRxiv - Genomics 2021Quote: ... hematopoietic colony-forming unit were automated counted and analyzed by STEMvision™ Human (STEMCELL Technologies). Differentiation frequency was calculated for each sorted population by number of resulting colonies/numbers of starting cells seeded.
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bioRxiv - Cancer Biology 2021Quote: ... CD3+ T cells were isolated using EasySep Human T Cell Isolation Kit (STEM CELL Technologies) and then cultured in CTS T Cell Expansion medium (Thermo ...
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bioRxiv - Physiology 2021Quote: ... The colonoids were maintained in Human IntestiCult Organoid Growth Medium (STEMCELL Technologies, Inc, Vancouver, BC). The human colonoids were transfected with the plasmids for fluorescent protein tagged human ALS-causing mutation SOD1G93A-GFP ...
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bioRxiv - Genomics 2021Quote: ... T cells were purified using the RosetteSep Human T Cell Enrichment Kit (Stem Cell Technologies) with the Lymphoprep density gradient medium and SepMate-50 tubes ...
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bioRxiv - Immunology 2022Quote: ... Total blood DCs were enriched with EasySep human pan-DC preenrichment kit (Stemcell Technologies 19251). DC enriched fraction were stained with antibodies specific for HLA-DR APCeFluor780 ...
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bioRxiv - Immunology 2020Quote: Cells were isolated using a human negative selection-based NK isolation kit (RosetteSep, STEMCELL Technologies). Purified NK cells were then cultured in stem cell serum-free growth medium (CellGenix GMP SCGM ...
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bioRxiv - Immunology 2021Quote: ... from healthy buffy coat donors and incubated overnight with human recombinant Interleukin 15 (STEMCELL Technologies). NK cells were incubated with immune complexes ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were activated with 1X ImmunoCult Human CD3/CD28 T Cell Activator (#10971, Stemcell Technologies) and cultured for 4 days ...
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bioRxiv - Immunology 2021Quote: Human peripheral blood from de-identified healthy donors was purchased from STEMCELL Technologies (Vancouver, Canada), which collects and distributes de-identified human blood products with consent forms ...
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bioRxiv - Immunology 2021Quote: ... T cells were further enriched using the EasySep Human T Cell Isolation Kit (STEMCELL Technologies), as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... according to the manufacturer’s protocol (EasySepTM Human Plasmacytoid DC Enrichment kit, STEMCELL Technologies Cat#19062). Enriched HSPC-pDCs were then primed for 1-3 days in RF10 (RPMI-1640 medium (Merck ...
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bioRxiv - Immunology 2021Quote: ... according to the manufacturer’s protocol (EasySepTM Human Plasmacytoid DC Enrichment kit, STEMCELL Technologies Cat#19062). Obtained cells were phenotypically validated by flow cytometry.
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bioRxiv - Cancer Biology 2021Quote: ... human CD41+ cells were isolated using the CD41+ selection TAC kit (Cat# 10450, StemCell Technologies).
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bioRxiv - Immunology 2022Quote: ... and activated using ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator (StemCell Technology, #10970) with or without 2µM of NU74411 for 48 hours.
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bioRxiv - Immunology 2022Quote: ... CD14+ monocytes were magnetically isolated (#100-0694 EasySep human positive selection kit II, STEMCELL Technologies) and the resulting cell suspension was passed through a 70 μm cell strainer.
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bioRxiv - Immunology 2022Quote: ... NK cells were removed from cultures (EasySep Human CD56 Positive Selection Kit II, StemCell Technologies). Remaining CD4 T cells were analyzed by flow cytometry to confirm removal of NK cells by staining for CD56 ...
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bioRxiv - Cancer Biology 2022Quote: ... CD8+ T cells were isolated using EasySep Human CD8+ T Cell Isolation Kit (Stemcell Technologies) by immunomagnetic negative selection according to manufacturer instructions.
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bioRxiv - Microbiology 2022Quote: ... CD4+ T cells were isolated using EasySepTM Direct Human T Cell Isolation Kit (Stemcell technologies) according to the manufacturer’s instructions and activated by incubation in the presence of 100 U/ml IL-2 (Sigma Aldrich ...
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bioRxiv - Developmental Biology 2022Quote: ... 5iLA naive human PSCs were dissociated into single cells by incubation with Accumax (Stemcell technologies) for 3 min at 37 °C ...
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bioRxiv - Microbiology 2022Quote: ... plated in matrigel and cultured with 500 μl/well human IntestiCult™ OGM (Stemcell Technologies). Medium was changed every 2-3 days and organoids were passaged once a week ...
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bioRxiv - Synthetic Biology 2022Quote: ... T cells were isolated using the RosetteSep Human T Cell Enrichment kit (Stem Cell Technologies) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2022Quote: ... with an additional granulocyte depletion step using the RosetteSep Human Granulocyte Depletion Cocktail (STEMCELL Technologies) as directed by the manufacturer ...
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Autologous humanized PDX modeling for immuno-oncology recapitulates the human tumor microenvironmentbioRxiv - Cancer Biology 2022Quote: ... CD34+ cells were positively selected using the EasySep Human CD34 Positive Selection Kit (STEMCELL Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: G-CSF Mobilized Human Peripheral Blood CD34+ Cells were purchased from StemCell Technologies (Catalog: 70060.1). Cells were thawed by resuspending in 10 mL of RPMI without FBS ...