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Citations for Stemcell Technologies :
451 - 500 of 1063 citations for Brucella Blood Agar with Hemin and Vitamin K 15x100mm plate 26ml fill since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Neonatal CD34+ HSPCs were obtained from cord blood of healthy donors and enriched using anti-CD34 immunomagnetic microbeads (Miltenyi Biotech and StemCell Technologies). All investigations were performed in accordance to the Declaration of Helsinki and informed consent was obtained according to local laws and regulations ...
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bioRxiv - Bioengineering 2022Quote: T cells were isolated from human donor whole blood samples by negative selection using the RosetteSep Human T Cell Enrichment Cocktail Kit (STEMCELL Technologies). Isolated T cells were seeded in a fresh complete ImmunoCult™-XF T cell expansion medium (STEMCELL Technologies ...
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bioRxiv - Systems Biology 2022Quote: ... and total peripheral blood mononuclear cells (PBMCs) were isolated by density centrifugation according to the manufacturers protocol (Lymphoprep, Stem Cell Technologies). Samples were cryopreserved at a concentration of 107 cells per milliliter in a solution of 90% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2020Quote: ... donor blood was diluted 1: 1 with phosphate buffered saline (PBS) and transferred into sterile SepMate 50 (STEMCELL Technologies, BC, Canada) as per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: Aliquots of fresh blood or frozen ficoll-separated peripheral blood cells were fractionated using EasySep Human Myeloid Positive Selection and EasySep Human T Cell Enrichment kits (StemCell Technologies. DNA was extracted from the fractionated subsets using MagMAX DNA Multi-Sample Ultra 2.0 Kit (ThermoFisher ...
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bioRxiv - Microbiology 2020Quote: ... CD4+ T cells were enriched from blood of healthy donors using RosetteSep Human CD4+ T cell enrichment cocktail (Stemcell Technologies, Canada) according to the manufacturer’s instructions followed by Ficoll density gradient centrifugation ...
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bioRxiv - Immunology 2021Quote: ... PBMCs were enriched from whole blood (day 10 after first, and day 7 after second vaccination) using direct PBMCs isolation kit (StemCell Technologies). For singe-cell antibody secretion and paired antibody sequencing studies ...
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bioRxiv - Immunology 2020Quote: ... PBMCs were separated from whole blood by density gradient centrifugation technique using Lymphoprep media (cat # 07851, STEMCELL technologies, Cambridge, MA, USA). PBMCs from CHOP patients were either frozen or used to isolate B cells immediately ...
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bioRxiv - Immunology 2021Quote: ... In vitro experiments were performed using human mononuclear cells from peripheral blood or synovial fluid (SF) isolated by centrifugation in density gradient medium (STEMCELL Technologies). Human monocytes were purified by positive selection with Human CD14 microbeads (Miltenyi) ...
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bioRxiv - Immunology 2020Quote: ... Memory human CD8+ T cells were purified from healthy donor blood samples using the EasySep Human Memory CD8+ T cell enrichment kit (StemCell Technologies), cells were routinely ∼90% CD8+RO+RA-.
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bioRxiv - Immunology 2020Quote: Total human CD8+ T cells were purified from healthy donor blood samples using the EasySep Negative human CD8+ T cell isolation kit (StemCell Technologies). CD8+ T cells were routinely ∼90% pure ...
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bioRxiv - Immunology 2021Quote: ... PBMC and plasma samples were isolated after whole blood centrifugation 1200 g for 10 minutes at room temperature in SepMate tubes (STEMCELL Technologies). Plasma was stored at -800C until analysis ...
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bioRxiv - Molecular Biology 2023Quote: ... Peripheral Blood Mononuclear Cells (PMBCs) were isolated from the buffy coat of whole blood (20 mL) using RosetteSep human enrichment cocktail (STEMCELL Technologies) and density gradient centrifugation as per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Red blood cells were lysed using ACK lysis buffer and mononuclear cells were isolated by density gradient using SepMate Tubes (StemCell Technologies) and Lymphoprep density gradient media (StemCell Technologies) ...
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bioRxiv - Microbiology 2023Quote: ... from participants with HIV-1 on ART were isolated from whole blood by density gradient centrifugation using LymphoprepTM and SepMateTM tubes (StemCell Technologies), and cryopreserved ...
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bioRxiv - Microbiology 2024Quote: Human polymorphonuclear neutrophils (PMNs) were isolated from the venous blood of healthy individuals following informed consent using a STEMcell isolation kit (STEMCELL Technologies). The purified neutrophils were resuspended in RPMI medium ...
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bioRxiv - Microbiology 2024Quote: ... was added to the blood sample before transferring to SepMate TM tubes prefilled with 4.5 mL of Lymphoprep (STEMCELL Technologies, Canada) density gradient medium ...
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bioRxiv - Microbiology 2024Quote: ... Naïve B cells were then isolated from adult blood using the EasySep Human Memory B Cell Isolation kit (STEMCELL Technologies #17864) to first remove CD27 positive memory cells ...
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bioRxiv - Cell Biology 2024Quote: ... mature NK cells were isolated from peripheral blood mononucleated cells with the EasySep™ Human NK Cell Enrichment Kit (STEMCELL Technologies). The human mesenchymal stromal cells (h-MSC ...
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bioRxiv - Cancer Biology 2023Quote: ... red blood cells were lysed using ACK lysis buffer and mononuclear cells were isolated by density gradient using SepMate Tubes (StemCell Technologies) and Lymphoprep density gradient media (StemCell Technologies) ...
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bioRxiv - Microbiology 2023Quote: ... from participants with HIV-1 on ART were isolated from whole blood by density gradient centrifugation using LymphoprepTM and SepMateTM tubes (StemCell Technologies), and cryopreserved ...
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bioRxiv - Cell Biology 2023Quote: Primary human CD4+ and CD8+ T cells were isolated from commercially available whole human blood (STEMCELL Technologies Inc., USA, Cat# 70507.1) with EasySep Human CD4+ or CD8+ T Cell Isolation Kits (STEMCELL Technologies Inc. ...
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Critical contribution of mitochondria in the development of cardiomyopathy linked to desmin mutationbioRxiv - Pathology 2023Quote: ... Blood samples from patient CIII were collected by Phenocell SAS (Grasse, France) and peripheral blood mononuclear cells (PBMC) were freshly collected using the SepMate (Stemcell Technologies) protocol ...
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bioRxiv - Neuroscience 2023Quote: ... To confirm Treg depletion spleen and lymph nodes were mashed through a 70 μm strainer and then treated with red blood cell lysis buffer (STEMCELL Technologies) for 2 min at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... pups were irradiated with sublethal dose (1 Gy) and thereafter engrafted with 1×105 human CD34+ cord blood cells (HLA-A24:02, Stemcell Technologies) through intrahepatic injection ...
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bioRxiv - Microbiology 2023Quote: Human peripheral blood mononuclear cells (PBMCs) were isolated from buffy coats from anonymized healthy blood donors by Ficoll density centrifugation using Lymphoprep (Stemcell Technologies). Buffy coats were provided by the Blood Donation Service Zurich ...
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bioRxiv - Molecular Biology 2022Quote: ... Neutrophils were isolated from 20 ml of blood with EasySep™ Human Neutrophil Isolation Kit (STEMCELL Technologies, British Columbia, Canada, 19666) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... A total volume of 10-20 ml of fresh blood was collected in EDTA separator tubes and peripheral blood mononuclear cells (PBMC) were immediately isolated using Lymphoprep and SepMate-50 (Stemcell Technologies) centrifugation tubes ...
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bioRxiv - Immunology 2023Quote: Different blood compartments (plasma, PBMCs, and RBCs) were separated by density gradient centrifugation using SepMate-15 tubes (STEMCELL Technologies, catalog #85415) to reduce blood processing time and user variability ...
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bioRxiv - Immunology 2023Quote: ... UCB mononuclear cells (UCBMC) and plasma were isolated after whole-blood centrifugation over LymphoPrep in SepMate tubes (STEMCELL Technologies, Vancouver, BC) following manufacturers’ protocols ...
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bioRxiv - Bioengineering 2023Quote: Human CD8+ T cells were purified from buffy coats from healthy donors at the NIH Blood Bank using EasySepTM Human CD8+ T Cell Isolation Kit (StemCell Technologies). Approximately 2×104 CD8+ T cells were plated in each well of a 96-well plate immediately following isolation ...
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bioRxiv - Molecular Biology 2024Quote: ... The diluted blood was carefully layered on top of 15 mL Lymphoprep™ density gradient medium (StemCell Technologies Inc., Vancouver, Canada), and centrifuged at 800xg for 15 minutes (room temperature ...
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bioRxiv - Microbiology 2024Quote: ... were isolated from fresh blood from anonymous healthy donors provided by the New York Blood Center using Ficoll-Paque PLUS (Cytiva) centrifugation and SepMate tubes (Stemcell Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... Blood was collected in EDTA microtainers and further processed to separate plasma and buffy coats using SepMate tubes (STEMCELL Technologies, 85415) and Ficoll-Paque PREMIUM 1.084 (Cytiva ...
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bioRxiv - Immunology 2024Quote: ... Human CD8+ T cells were purified from healthy donor blood samples using the RosetteSep Human CD8+ T Cell Enrichment Cocktail (StemCell Technologies). For cloning ...
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bioRxiv - Molecular Biology 2024Quote: ... from participants with HIV-1 on ART were isolated from whole blood by density gradient centrifugation using LymphoprepTM and SepMateTM tubes (StemCell Technologies), and cryopreserved ...
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bioRxiv - Cancer Biology 2024Quote: ... Human peripheral blood mononuclear cells from healthy donors were thawed from cryostorage in ImmunoCult-XF medium (Stem Cell Technologies # 100-0956) containing 100 U ml−1 IL-2 in the presence of ImmunoCult™ CD3/CD28/CD2 T cell activator (Stem Cell Technologies # 10970 ...
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bioRxiv - Bioengineering 2021Quote: ... The plates were rinsed with Anti-Adherence Solution (#07010, Stemcell Technologies) and cells were plated at 9000cells/well density ...
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bioRxiv - Cell Biology 2021Quote: ... coated plates in hESC or mTeSR1 medium (Stem Cell Technologies, 05850). This serial subcloning was performed until remnants of Sendai virus RNA were no longer detected and the morphology looked stable ...
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bioRxiv - Immunology 2021Quote: ... ESC were maintained on Matrigel coated plates in mTeSR1 (STEMCELL Technologies) with daily medium changes ...
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bioRxiv - Cell Biology 2022Quote: ... and cultured in 24- well plates in SFEM (Stem Cell Technologies) plus 100 ng ml−1 mTPO (Novoprotein ...
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bioRxiv - Cell Biology 2022Quote: ... USA)-coated culture plates with mTeSR1 medium (Stemcell Technologies, Vancouver, BC, Canada) as reported previously33 ...
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bioRxiv - Neuroscience 2021Quote: ... coated 6-well plates and maintained in mTeSR1 medium (STEMCELL Technologies). Nociceptor differentiation was performed as previously published (18 ...
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bioRxiv - Genetics 2022Quote: ... AggrewellTM plates were treated with Anti-Adherence Rinsing solution (Stemcell Technologies) as per manufacturer instructions ...
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bioRxiv - Neuroscience 2022Quote: ... 6-well plate for 14 days using Brainphys (Stem Cell Technologies) 1X B27 Supplement (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2021Quote: ... All human iPSCs were cultured on Matrigel (Corning)-coated 6-well plates with mTeSR™1 medium (StemCell Technologies). Full-volume medium replacement with mTESR1 was performed daily ...
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bioRxiv - Cell Biology 2023Quote: ... coated tissue culture treated plates in mTeSR1 medium (STEMCELL Technologies 85850). The cells were split using TrypLE™ Express (Thermo Fisher 12605028 ...
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bioRxiv - Neuroscience 2023Quote: ... coated 6-well plates and maintained in mTeSR1 medium (STEMCELL Technologies). Nociceptor differentiation was performed as previously published.
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bioRxiv - Neuroscience 2023Quote: ... Each plate was then split 1:2 using ReLeSR (StemCell Technologies), with half of the cells used for DNA extraction for Sanger sequencing ...
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bioRxiv - Neuroscience 2023Quote: ... 1.2×106 iPSCs were seeded in AggreWell800 plates (Stem cell technologies) in EB medium consisting of DMEM/F-12 supplemented with 20% Knockout Serum Replacement ...