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Citations for Stemcell Technologies :
451 - 500 of 1467 citations for 6 4 Methyl 1 piperazinyl N 5 methyl 1H pyrazol 3 yl 2 1Z 2 phenylethenyl 4 pyrimidinamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... the isolated intestinal tissue was cut into ∼3-to 5-mm pieces and incubated in Gentle Cell Dissociation Reagent (Stem Cell Technologies, Vancouver, Canada). After removal of the Gentle Cell Dissociation Reagent ...
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bioRxiv - Cancer Biology 2021Quote: ... Sigma for 2 min at room temperature and then incubated in neutralization buffer supplemented with NH4Cl (0.8%, StemCell Technologies) to remove red blood cells ...
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An in vitro neuronal model replicating the in vivo maturation and heterogeneity of perineuronal netsbioRxiv - Neuroscience 2022Quote: ... the media was replaced with BrainPhys™ medium with 2% (v/v) SM1 and antibiotic-antimycotic (StemCell Technologies #5792). From 14 DIV ...
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bioRxiv - Neuroscience 2022Quote: ... neural induction was initiated using a chemically defined neural base medium (NbM) supplemented with 2 µM Dorsomorphin (StemCell Technologies), 3 µM CHIR990021 (Sigma ...
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bioRxiv - Bioengineering 2023Quote: ... and were differentiated for 2 days using 100 ng/ml phorbol myristate acetate (PMA) (StemCell Technologies, Cambridge, MA, USA). To study the adhesion dynamics of sickle RBCs under shear flow and hypoxia ...
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bioRxiv - Cancer Biology 2023Quote: ... the cells were washed twice with PBS (+ 2%FBS) and with a red blood cell lysis buffer (0.8% NH4Cl, 0.1mM EDTA, STEMCELL technologies). Thereafter ...
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bioRxiv - Developmental Biology 2024Quote: ... and 2 µM A83-01, an inhibitor of TGFβ type I receptors (ALK4, ALK5, ALK7) (Stem Cell Technologies, 72022) for 5 days in vitro to induce embryoid body (EB ...
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bioRxiv - Cancer Biology 2024Quote: ... with base media consisting of Iscove’s modified Dulbecco’s medium (IMDM) supplemented with 2% human blood plasma (STEMCELL Technologies, #70039), 3% human AB serum (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... we changed the medium to a 1:1 mixture of KSR and N2B medium (DMEM F12, Thermo Fisher 11320033, with 1% GlutaMAX, 3% dextrose, N2-Supplement B, StemCell Technologies 07156 ...
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bioRxiv - Biophysics 2024Quote: ... hiPSCs from N=1 donor were differentiated to definitive endoderm by using the STEMdiff™ Definitive Endoderm Kit (STEMCELL Tech., Vancouver, BC, Canada). Subsequently ...
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bioRxiv - Immunology 2023Quote: ... For 5-azacytidine (5-aza; Stem Cell Technologies) and trehalose 6,6’-dimycolate (TDM ...
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bioRxiv - Genomics 2020Quote: NSTcPA buffer (1 mL) was pipetted into one well of a 6-well plate (Stem Cell Technologies, cat. no. 38015) on ice ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were maintained in NbM for 6 days after which they were passaged with 1 U/mL Dispase (StemCell Technologies) and cultured in NbM supplemented with 0.1 µM Retinoic Acid (RA ...
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bioRxiv - Cancer Biology 2022Quote: ... and Interleukin-6 (IL6, StemCell Technologies, Cat#78050).
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bioRxiv - Cell Biology 2024Quote: ... interleukin (IL) 6 and IL 11 (StemCell Technologies). Cultures were maintained at 37°C in a 5% CO2 humidified atmosphere for 14 days with media changes on days 5 ...
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bioRxiv - Developmental Biology 2021Quote: ... The cell pellet was resuspended into HF buffer (HBSS, 2% fetal bovine serum, 10mM Hepes pH 7.2) and NH4Cl (StemCell Technologies). The cell pellet was recovered after 5 min of centrifugation at 1000rpm and resuspended in organoid medium for organoid culture ...
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bioRxiv - Neuroscience 2020Quote: ... Transduced hiPSCs were selected and expanded in the presence of 2 μM puromycin and cryo-stored in mFreSR (STEMCELL Technologies) or further differentiated to OPCs as described below.
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bioRxiv - Immunology 2019Quote: ... Isolated T cells were cryopreserved at 2×107 T cells per vial in CryoStor CS10 cryopreservation medium (Stem Cell Technologies).
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bioRxiv - Neuroscience 2022Quote: ... For comparing to hCO cultured in STEMdiff™ Neural Organoid Basal Medium 2 (hCO + STEMdiff™ NOBM, STEMCELL Technologies, 08620), the remaining organoids in each well were imaged at day 12 on a REVOLVE microscope (ECHO ...
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bioRxiv - Developmental Biology 2019Quote: Placenta cell suspensions were suspended in Iscove’s MDM with 2% fetal bovine serum and cultured using a MethoCult GF M3434 Optimum kit (STEMCELL Technologies). 10,000 cells from each sample were plated in 35-mm culture dishes (STEMCELL Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were harvested and blocked with CD32 antibody (2 µl per 50,000 cells in 100 µL, STEMCELL Technologies Catalog #60012) for 15 minutes at 4C ...
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bioRxiv - Neuroscience 2023Quote: ... half of the medium was refreshed weekly with BrainPhys medium (BP) supplemented with 2% NeuroCult SM1 neuronal supplement (STEMCELL Technologies) and 1% penicillin/streptomycin.
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bioRxiv - Immunology 2023Quote: B cells subsets were obtained from WT or IL17 −/− naïve mice or from mice 14 d after alloantigen immunization (2 × 10 7 mitomycin C–treated BALB/c splenocytes i.p.) by negative selection (EasySep; StemCell Technologies). Purity of the B cell subset was greater than 98% ...
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bioRxiv - Cell Biology 2020Quote: Flow cytometer-sorted murine or human HSCs were resuspended in 4 mL complete MethoCult GF M3434 medium or MethoCult™ H4435 Enriched (STEMCELL Technologies, 03434 and 04435) thawed at RT and 1 mL of the suspension was placed into 35 mm dishes in triplicates ...
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bioRxiv - Cancer Biology 2022Quote: CD8+ T-cells were aseptically isolated from the spleen and lymph nodes of C57BL/6 or FVB (6-8 weeks old) mice using the negative selection Easysep mouse CD8+ T-cell isolation kit (StemCell Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... 3 µM CHIR99021 (Stemcell Technologies), 1 µM PD0325901 (Stemcell Technologies) ...
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bioRxiv - Developmental Biology 2023Quote: ... 3 μM CHIR99021 (STEMCELL Technologies), 1 μM PD0325901 (STEMCELL Technologies) ...
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bioRxiv - Immunology 2024Quote: ... + 3 µM CHIR9902 (Stemcell Technologies) + 10 µM SB-431542 (Stemcell Technologies ...
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bioRxiv - Bioengineering 2019Quote: ... and cultured at a concentration of 1×103 cells mL−1 in one well of a 6-well plate (#1008; Becton-Dickinson) with 1 mL MethoCult GF+ semisolid medium (#4435, STEMCELL Technologies). Colonies were counted after 14 days of incubation using an inverted microscope by three individuals.
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bioRxiv - Cancer Biology 2023Quote: ... 1.1 mL of methocult-cell suspension was plated into a 6-well plate using 16-gauge blunt-end needles (STEMCELL Technologies). After 14 days ...
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bioRxiv - Cell Biology 2024Quote: ... Semi-solid MC3434 cell mixtures were plated at 1 mL mixture/well in a 6-well meniscus-free dish (SmartDishTM, STEMCELL Technology) using a 3cc syringe and a blunt-end 16-gauge needle (STEMCELL Technology) ...
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Genetically engineered microglia-like cells have therapeutic potential for neurodegenerative diseasebioRxiv - Neuroscience 2021Quote: ... Lineage negative cells were transduced with a lentiviral vector in a cell incubator at 2 ×106 cells/mL in either StemSpan SFEM II (STEMCELL Technologies) or StemMACS HSC Expansion Media (Miltenyi Biotec ...
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bioRxiv - Developmental Biology 2019Quote: Sorted cell populations were counted and plated at 5000 cells into 2 ml of methylcellulose medium (Human enriched H4435, Stemcell Technologies). Cells were incubated in the assay for 14 days and then scored.
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bioRxiv - Cell Biology 2019Quote: ... insulin-like growth factor 2 (IGF2; 20 ng/ml) and fibroblast growth factor (FGF; 10 ng/ml) (BioLegend or StemCell Technologies) along with 5ug/ml polybrene (Sigma ...
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bioRxiv - Cancer Biology 2021Quote: ... 4T1 tumors were extracted and minced with sterile razor blades and incubated for 2 h in the presence of Collagenase (1,000 units per sample) in sterile Epicult media (Stem Cell Technology). Cells were washed with sterile filtered PBS supplemented with 1% bovine serum albumin (PBS-BSA 1% ...
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bioRxiv - Developmental Biology 2022Quote: ... hESCs were plated onto Matrigel at a density of 2×105 cells/cm2 in E8 medium containing 10 µM ROCK inhibitor (STEMCELL Technologies) and cultured overnight ...
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bioRxiv - Bioengineering 2020Quote: ... On days 8-15+ cell culture media was exchanged every 2 days with 15 mL of Cardiomyocyte Maintenance Medium (STEMCELL Technologies). On day 30 ...
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bioRxiv - Cell Biology 2022Quote: CFU assays in PHZ-treated and retrovirally-infected spleen progenitors were conducted using spleen suspensions at 2 × 104 cells/mL mixed with Methocult M3434 (STEMCELL Technologies) containing Epo ...
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bioRxiv - Immunology 2022Quote: ... FACS sorting was performed on an Influx-2 on pre-enriched CD4+ T cells (enrichment using an EasySep CD4+ T cell isolation kit (StemCell Technologies)) ...
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bioRxiv - Neuroscience 2022Quote: ... human PDGF-AA (10 ng/ml) and PDGF-BB (10 ng/ml; Shenandoah) and heparin (2 ng/ml; Stem Cell Technologies).
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bioRxiv - Developmental Biology 2022Quote: ... cells were collected by Trypsin and half of the well equivalent was plated in 2 ml of methylcellulose medium (Human enriched H4435, Stemcell Technologies). Cells were incubated in the assay for 14 days and then scored.
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bioRxiv - Cell Biology 2022Quote: ... Cell pellets (containing 2 × 106) were cultured for 21 days in MesenCult™-ACF Chondrogenic Differentiation Kit (STEMCELL TECHNOLOGIES, Inc, Canada) to induce differentiation ...
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bioRxiv - Developmental Biology 2022Quote: ... From day 7 - 9 of differentiation cells were cultured in cSFDM containing 2 μM Retinoic acid and 0.5 μg/ml purmorphamine (Stemcell Technologies, #72202), and then subsequently in cSFDM with 2 μM Retinoic acid until day 11-13 of differentiation ...
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bioRxiv - Neuroscience 2023Quote: ... 550,000 neurons were seeded in 6 well plates coated with 1 mg/mL polyLysine were cultured in conditioned Neurobasal medium supplemented with 2 mM L-glutamine and NeuroCult SM1 Neuronal supplement (STEMCELL Technologies) half renewed every 3 days with 3.4mM AraC ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 x 104 sorted cells were plated in triplicate in serum-free media containing StemSpan™ Megakaryocyte Expansion Supplement (STEMCELL Technologies) for 10-12 days ...
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bioRxiv - Immunology 2023Quote: ... 200-500 μm cystic EBs were selected under a dissecting microscope and transferred onto adherent tissue culture plates (∼2.5 EBs/cm2) for cultivation in Hematopoietic Differentiation (HD) medium (APEL 2, Stem Cell Tech, 05270 ...
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bioRxiv - Cell Biology 2023Quote: ... 2×105 freshly isolated cells from digests of aorta or specified tissue were plated in methylcellulose (MethoCult GF M3434, StemCell Technologies), in duplicate ...
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bioRxiv - Cell Biology 2024Quote: ... cells were collected by Trypsin and half of the well equivalent was plated in 2 ml of methylcellulose medium (Human enriched H4435, Stemcell Technologies). Cells were incubated in the assay for 14 days and then scored.
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bioRxiv - Cell Biology 2024Quote: Whole blood was collected from 12M and 20M SM/J and BL6 mice via intracardiac puncture and diluted into an equal volume of Dulbecco’s PBS + 2% FBS (StemCell Technologies, 07905). According to the manufacturer’s specifications ...
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bioRxiv - Neuroscience 2024Quote: ... media was removed and neural induction media (NIM; Supplementary Table 2) supplemented with small molecules 10 µM SB431542 (StemCell Technologies, #72146) and 0.1 µM LDN193189 (StemCell Technologies ...