Labshake search
Citations for Stemcell Technologies :
451 - 500 of 1285 citations for 5 Chloro 1 vinyl 2 pyridone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Primary mouse microglia and M-MØP cells were exposed at set time points to anti-FcγRII/III (2.4G2) antibody at 5 µg/ml (Stemcell technologies), LPS (50 ng/ml ...
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bioRxiv - Cell Biology 2022Quote: ... mESCs were cultured in feeder free conditions with 5% CO2 at 37°C on 0.1% gelatin (StemCell Technologies, 7903) coated plates in 2i medium supplemented with 2% mES certified FBS (Gibco 16141061) ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were seeded at 5 × 105 cells/ml and grown in Neurocult mouse neurobasal medium (Stem Cell Technologies, 5700) supplemented with 10 ng/ml hEGF (Lonza ...
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bioRxiv - Microbiology 2020Quote: ... 5×106 naïve CD4+ T cells from HIV− or HIV+ donors were isolated by magnetic negative selection (StemCell Technologies), then cultured at 106 cells/mL in 96-well plates using R-10 media (RPMI-1640 media supplemented with 10% FBS ...
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bioRxiv - Bioengineering 2021Quote: ... coated tissue culture plates and passaged regularly as cell aggregates every 4-5 days using ReLeSR (Stemcell Technologies, 05872).
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bioRxiv - Molecular Biology 2020Quote: ... CD34+ HSPCs were cultured at 2.5×105–5×105 cells/mL in StemSpan SFEM II (STEMCELL Technologies, Vancouver, Canada) base medium supplemented with stem cell factor (SCF)(100ng/mL) ...
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bioRxiv - Neuroscience 2023Quote: ... the hiNPCs were singularized with Accutase for 10 min at 37 °C and 5 % CO2 (#07920, Stemcell Technologies, Canada) and centrifuged (300 g ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 x 105 CD3+ T cells were plated in 0.5 ml T cell expansion medium (Stemcell Technologies, Cologne, Germany) with 50 IE/ml IL-2 ...
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bioRxiv - Cancer Biology 2024Quote: ... mTeSR was changed every other day and cells were passaged every 3–5 days using ReLeaSR (StemCell Technologies #05872). All cell cultures were maintained in a humidified incubator with 5% CO2 at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... mesoderm was carried out for 5 days and ectoderm for 7 days using STEMdiff Trilineage Differentiation Kit (STEMCELL Technologies). Alternatively ...
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bioRxiv - Cell Biology 2023Quote: ... After 5 weeks the H5100 medium was replaced with Methocult H4434 Classic methylcellulose medium (100µl/ well, STEM CELL technologies). Wells that contained at least one colony after 10-14 days were considered positive.
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bioRxiv - Cancer Biology 2022Quote: ... We grew cells in a 5% CO2 incubator in a serum-free NeuroCult NS-A basal medium (STEMCELL Technologies) supplemented with NeuroCult™ Proliferation Supplement (STEMCELL Technologies) ...
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bioRxiv - Bioengineering 2022Quote: iPSC lines were maintained and expanded at 37°C in 5% CO2 in mTeSR™Plus medium (Stemcell Technologies) on 1:100 diluted Geltrex (Thermo Fisher)-coated plates with daily medium changes and passaging every 3-4 days with releasing agent ReLeSR (Stemcell Technologies).
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bioRxiv - Cell Biology 2022Quote: ... Red blood cells were removed using the ammonium chloride solution: 5 ml of 0.8% Ammonium Chloride (StemCell Tech. 07800) was added to the suspension and incubated for 10 min with intermittent mixing ...
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bioRxiv - Developmental Biology 2022Quote: ... Dispase II at 5 U/ml stock solution in Hanks’ balanced saline was from Stemcell Technologies (cat. no. 07913). The following chemicals were prepared and used according to the manufacturers’ guidelines ...
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bioRxiv - Immunology 2023Quote: ... Cells were pelleted at 300g for 5 mins and RBC lysed using Ammonium Chloride Lysis Buffer (Stem Cell Technologies) as per manufacturer protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... Dispase II at 5 U/ml stock solution in Hanks’ balanced saline was from Stemcell Technologies (cat. no. 07913). The following chemical compounds were prepared and used according to the manufacturers’ guidelines ...
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bioRxiv - Cancer Biology 2024Quote: ... Two million human tumor cells were cultured under mammosphere conditions using 5 mL of complete Mammocult medium (StemCell Technologies) in ultra-low attachment plates (Corning ...
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bioRxiv - Synthetic Biology 2024Quote: ... coated tissue culture plates and passaged regularly as cell aggregates every 4-5 days using RelesR (STEMCELL Technologies, 05872). Identity of H1 cells was authenticated by the supplier ...
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bioRxiv - Genomics 2024Quote: ... Organoids followed by incubation for 5 minutes at 37°C with resuspension with Accutase (Stemcell Technology, Cat No.07922) were dissociated into 10-15 cell clusters by pipetting 10-20 times ...
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bioRxiv - Developmental Biology 2024Quote: RUES2 hESCs were cultured in a humidified incubator at 37°C and 5% CO2 in mTeSR1 (StemCell Technologies, 85850) on growth factor-reduced Matrigel-coated (Corning ...
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bioRxiv - Developmental Biology 2021Quote: Unsorted cells at day 6 (4+2) of differentiation were cultured in MethoCult 04434 (Stem Cell Technologies) in 35 mm dishes ...
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bioRxiv - Bioengineering 2022Quote: ... Both UC3-4 and UCS-2 hiPSCs were frozen in mFreSR medium (Stem Cell Technologies, Vancouver, Canada) and stored under cryogenic conditions in a vapor phase liquid nitrogen dewar ...
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bioRxiv - Cancer Biology 2021Quote: ... For the first 2 days cells were also exposed to 3 μM CHIR-99021 (Stem Cell Technologies). From day 4 for 5 days ...
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bioRxiv - Bioengineering 2023Quote: ... the media was fully replaced with 2 mL Hematopoietic Media B (STEMdiff Hematopoietic Basal Media (StemCell Technologies) + STEMdiff Hematopoietic Supplement B (200x ...
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bioRxiv - Cell Biology 2023Quote: ... Sorted cells were plated in Methocult optimum media (2-3 X 103 cells/well; Stemcell Technologies, H4034) according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... the cell pellet from one 60 mm dish was resuspended in 2 mL mFreSR medium (Stemcell Technologies) to freeze two vials of the cells by putting them in an isopropanol freezing container at –80°C overnight before transferring them into liquid nitrogen.
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bioRxiv - Cell Biology 2023Quote: ... Cells were expanded by culturing in RPMI 1640 with B27 with 2 μM of CHIR99021 (Stemcell Technologies). Media was replaced daily for 6-7 days or until cells reached confluency ...
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bioRxiv - Neuroscience 2024Quote: ... and Lancaster and Knoblich [2] using the commercially available STEMdiff™ Cerebral Organoid Kit from StemCell Technologies. Organoids were generated according to the guidelines from the manufacturer with a few modifications enabling large-scale culturing of appropriate organoid numbers (Figure S1) ...
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bioRxiv - Developmental Biology 2024Quote: ... The EBs were then incubated in 2 mL Gentle Cell dissociation reagent (Stem Cell Technologies, #100-0485) in a 37°C water bath for 15 minutes followed by vigorous trituration until they formed a single cell solution ...
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bioRxiv - Cancer Biology 2024Quote: ... T cells were cryopreserved at 2 × 107 cells per ml in CryoStor CS10 cryopreservation medium (StemCell Technologies) until use ...
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bioRxiv - Cancer Biology 2024Quote: ... EB were transferred in Neural Induction Medium (DMEM/F12 1% Glutamax containing 1% [v/v] N2 [Gibco Cat#17502048], 1% [v/v] MEM-NEAA, 1μg/mL Heparin [Stem Cell Technologies Cat#07980], 1% [v/v] Anti-Anti). Seven days after their initiation ...
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Single-nucleus multi-omics identifies shared and distinct pathways in Pick’s and Alzheimer’s diseasebioRxiv - Neuroscience 2024Quote: ... in Cortical Neuron Culture Medium 1 (CM!): 1:1 Knockout DMEM/F12: BrainPhys neuronal medium without Phenol-Red (STEMCELL Technologies); B27 supplement ...
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bioRxiv - Cell Biology 2020Quote: ... domes diluted 1:1 with advanced DMEM/F12 media (Stemcell Technologies, UK), with 50 μl domes per well ...
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bioRxiv - Physiology 2022Quote: ... then medium changed to 1:1 cFAD+RI:Pneumacult ALI (Stemcell Technologies, UK) for 2 days to ensure monolayers were 100% confluent ...
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bioRxiv - Neuroscience 2024Quote: ... IgG1 κ Isotype (1:188, STEMCELL Technologies, 60070AD.1, 0.2 ug/µL) was used ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were suspended in Hanks Balanced Salt Solution (HBSS) supplemented with 5% human serum and 1.5 µg/mL anti-human CD32 antibody (Clone IV.3; STEMCELL Technologies) and then stained with designated antibodies for 1-2 hours on ice prior to sorting on a Becton Dickinson FACSAria™ Fusion or FACSAria™ III sorter ...
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Pluripotent stem cell SOX9 and INS reporters facilitate differentiation into insulin-producing cellsbioRxiv - Developmental Biology 2021Quote: ... centrifuged at 50 x g for 5 min and subsequently dissociated by incubation with gentle cell dissociation solution (StemCell Technologies) for 15 min and additional T/E for 10 min ...
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bioRxiv - Bioengineering 2021Quote: ... The microcapsules carrying HUES-8 cells were collected into 15 mL tube filled with 5 mL E8 medium (STEMCELL Technologies), then distributed into a 6 well-plate at density of 2000 capsules/well and incubated at 37 □ with 5% CO2 ...
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bioRxiv - Cell Biology 2022Quote: ... They were patterned into definitive endoderm using the STEMdiff Definitive Endoderm Kit per manufacturer’s instructions over 5 days (StemCell Technologies). Cells were passaged on day 5 of differentiation using GCDR ...
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bioRxiv - Cancer Biology 2022Quote: ... freshly dissected prostate tissues were dissociated in DMEM containing 5% FBS and 1x collagenase/hyaluronidase (STEMCELL Technologies, Vancouver, BC, Canada), followed by trypsinization with 0.25% trypsin-EDTA (Gibco ...
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bioRxiv - Developmental Biology 2022Quote: ... organoids were treated from days 3 - 5 with ROCKi-free neural differentiation medium supplemented with retinoic acid (RA) (Stemcell Technologies) at 0.25 μM and smoothened agonist (SAG ...
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bioRxiv - Neuroscience 2022Quote: hiPSCs were cultured at 37ºC and 5% CO2 in a humidified incubator (BINDER C150) in mTeSR1 medium (STEMCELL Technologies, #85850) on Matrigel (Corning ...
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bioRxiv - Genomics 2021Quote: ... The medium was changed every day and the cells were passed every 5-6 days using ReLeSR (Stem Cell technologies). 24h prior transfection ...
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bioRxiv - Molecular Biology 2023Quote: ... Cardiomyocyte specification was completed by 3 days of culture in RPMI B27-media with 5 μM IWP-4 (STEMCELL Technologies), followed by another 7 days of culture with RPMI B27+ (RPMI 1640 GlutaMAX + 2% B27 supplement with insulin (ThermoFisher) ...
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bioRxiv - Immunology 2023Quote: ... The cells were incubated at 37°C and 5% v/v CO2 until confluency in PneumaCultTM-ExPlus media (STEMCELL Technologies) for 4-7 days before being switched to ALI culture conditions by removing the apical media and feeding the basal side with PneumaCultTM ALI medium (STEMCELL Technologies) ...
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bioRxiv - Cell Biology 2023Quote: ... The medium was replaced on d1 with RPMI/B27™ minus insulin supplemented with 5 µM IWP-4 (STEMCELL Technologies). On d3 medium change was performed using RPMI/B27™ minus insulin without any supplementation ...
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bioRxiv - Neuroscience 2022Quote: ... NPCs were plated at 1.25×5 cells/cm2 on Matrigel hESC-qualified Matrix-coated wells and maintained in Neural Progenitor medium (StemCell Technologies) with daily complete media change ...
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bioRxiv - Physiology 2022Quote: ... Cells were cultured in mTESR1 media at 37°C at 5% CO2 and passaged using accutase (Stemcell Technologies, Cat. # 07920) and media supplemented with Y-27632 2HCl (ROCK ...
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bioRxiv - Immunology 2024Quote: ... mice and bone marrow progenitors were plated on non- tissue treated dishes and differentiated into BMDM during a 6-day culture in complete Dulbecco’s modified Eagle’s medium (DMEM) containing 5% FBS and supplemented with 60 ng/ml recombinant murine M-CSF (Stemcell Technologies) in a water saturated atmosphere at 37°C ...