Labshake search
Citations for Stemcell Technologies :
451 - 500 of 511 citations for 2 4 Dimethyl 4' 4 methylpiperazinomethyl benzophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Physiology 2022Quote: ... Proteases were neutralized with medium containing 10% FBS and resulting suspension of cells were incubated in a flask at 37°C 5% CO2 for 2 hours in PneumaCult-Ex Plus medium (Cat. 05040, Stemcell Technologies) with 100 U/mL penicillin and 100 μg/mL streptomycin to remove non-epithelial cells ...
-
bioRxiv - Cancer Biology 2023Quote: The six basal- and luminal-like cell lines were disassociated with 0.25% trypsin and added individually to wells of a 6 well Ultra-low attachment plate in 2 mL Mammocult culturing media (STEMCELL Technologies). Accutase (STEMCELL Technologies ...
-
bioRxiv - Bioengineering 2023Quote: ... The PBMC pellet was resuspended in PBS supplemented with 2% FBS and 1 mM EDTA and monocyte isolation was performed using the EasySepTM human monocyte isolation kit (Stemcell Technologies) according to manufacturer instructions ...
-
bioRxiv - Cancer Biology 2023Quote: ... bone marrow aspirates were diluted 1:1 with PBS (+ 2% FBS) and layered on a Lymphoprep gradient using SepMate tubes (STEMCELL Technologies). After centrifugation ...
-
bioRxiv - Immunology 2024Quote: ... were irradiated with 2 Gy by 177Lu diluted in 3 mL of ImmunoCult-XF T cell Expansion Medium (cat. no. 10981; STEMCELL Technologies) supplemented with 50 IU/mL of IL-2 over 3 days and then examined by flow cytometry for viability as previously described 25.
-
bioRxiv - Cell Biology 2024Quote: ... IL-11 and SCF cultures:2 Equal volume of medium was added to each well to a final concentration of 10% FBS (STEMCELL Technologies), 1% penicillin/streptomycin (Sigma-Aldrich) ...
-
bioRxiv - Cancer Biology 2024Quote: Tissue samples of ovarian tumours were cut into ∼1-2 mm3 pieces using sterile scalpels and digested with collagenase type IV (STEMCELL Technologies) for 2 hours at 37°C on a rotator ...
-
bioRxiv - Genomics 2021Quote: ... after which cells were replated in E8 containing p160-Rho-associated coiled-coil kinase (ROCK) inhibitor Y-27632 (2 μM; Stemcell Tech, 72307).
-
bioRxiv - Neuroscience 2020Quote: ... Medium was refreshed every 2-3 days and cells were passaged twice a week using an enzyme-free dissociation reagent (ReLeSR, Stem Cell Technologies).
-
bioRxiv - Bioengineering 2021Quote: hiPSC-derived endothelial cells (hECs) were differentiated as previously described.[85] hiPSCs were subjected to 5 μM CHIR99021 in STEMdiff APEL 2 Medium (STEMCELL Technologies, 05270) for 24 hours ...
-
bioRxiv - Cancer Biology 2022Quote: 2,500 cells were seeded into each well of an ultra-low attached 6-well plate in 2 mL complete MammoCultTM Medium (Stem Cell Technologies) based upon the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2021Quote: ... 1 or 2 EBs were transferred to a well of a 24-well ultra-low attachment plate containing Induction Medium (Stem Cell Technologies). The EBs were kept in the induction Medium for 2 days and next they were transferred into Matrigel (Corning ...
-
bioRxiv - Neuroscience 2022Quote: ... IPSCs were then collected and spun down at 500rpm for 1-2 minutes and resuspended in 2mL of mTeSR Plus media with 20 µM ROCK inhibitor Y-27632 (Stemcell Technologies # 72304). Matrigel ...
-
bioRxiv - Neuroscience 2021Quote: ... Medium was refreshed every 2-3 days and hiPSCs were passaged twice per week using an enzyme-free reagent (ReLeSR, Stem Cell Technologies).
-
bioRxiv - Cancer Biology 2021Quote: ... All GSC lines and human neural stem cells were maintained as neurospheres using ultra low-adherence culture dishes and dissociated 1-2 times per week with Accutase (StemCell Technologies 07922). Primary human astrocytes were cultured adherently on plates coated with Geltrex (Life Technologies A1413202) ...
-
bioRxiv - Bioengineering 2022Quote: ... were either coated with 70 µL of 2% agarose in water or PBS or treated for 20 min with 100 µL of anti-adherence rinsing solution (StemCell Technologies 07010). The wells were washed with PBS ...
-
bioRxiv - Immunology 2021Quote: Whole blood was collected in heparin containing vacuutubes and 12μL of 0.5M EDTA with 1mL of PBS+2% FBS and 50μL of RBC of EasySep RBC Depletion spheres (EasySep ™ RBC Depletion Reagent: 18170, Stem Cell Technologies) were added ...
-
bioRxiv - Immunology 2022Quote: The collected blood was 1:1 diluted with 2% FBS and was added to 7ml of Lymphoprep™ density gradient medium in SepMate-15 tubes (StemCell Technologies). The red blood cells ...
-
bioRxiv - Genomics 2023Quote: ... liver samples in cohort 2 were reduced of dead cell burden using the EasySep Dead Cell Removal (Annexin V) Kit (STEMCELL Technologies, 17899) to improve cell viability to >70% ...
-
bioRxiv - Cell Biology 2023Quote: ... Medium was refreshed every 2-3 days and cells were passaged twice per week using an enzyme-free reagent (ReLeSR, Stem Cell Technologies).
-
bioRxiv - Neuroscience 2024Quote: ... hESC colonies are passaged and small clusters are plated onto a 6 well plate at a density of ∼ 1-2 x 104 cells/well in TeSR-E8 media (StemCell Technologies, #5990). Following 24 hrs ...
-
bioRxiv - Developmental Biology 2023Quote: PP cells were dissociated using TrypLE Express at 37°C for 2-3 mins and seeded in microwells of AggreWell 800 plates (STEMCELL Technologies, 34825), using the recommended procedure by the supplier ...
-
bioRxiv - Bioengineering 2023Quote: ... Stages 1 and 2 used basal media comprised of 1:1 mixture RPMI and DMEM F-12 supplemented with BSA and SM1 (Stem Cell Technology) or B27 (ThermoFisher ...
-
bioRxiv - Cell Biology 2023Quote: Mature organoids are collected by rinsing the culture well with 1-2 ml ice cold DMEM++ (= DMEM/F12 + 15 mM HEPES (Stem Cell Technologies) + 1x GlutaMAX (Thermo Fisher Scientific ...
-
bioRxiv - Genomics 2023Quote: 50 U/mL IL-2 (Amerisource Bergen, #10101641) and stimulated with 6.25 uL/mL ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator (STEMCELL Technologies, #10990). Following activation and electroporation ...
-
bioRxiv - Molecular Biology 2023Quote: ... and plated (200,000 cells/well) on low attachment 12-well plates coated with poly-2-hydroxyethyl methacrylate in mTeSR1 Plus (STEMCELL Technologies, Vancouver, Canada) medium with 10 μM Y27632 (Selleckchem ...
-
bioRxiv - Neuroscience 2022Quote: ... human PDGF-AA (10 ng ml−1) and PDGF-BB (10 ng ml−1; Shenandoah) and heparin (2 ng ml−1; Stem Cell Technologies). For all patient-derived cultures ...
-
bioRxiv - Immunology 2022Quote: ... blood was resuspended in PBS/2%FBS/EDTA and separated using Ficoll-Paque Plus (Cytiva, 17144002) density gradient centrifugation in SepMate50 tubes (StemCell Technologies, 85450), followed by two washes in PBS/2%FBS/EDTA ...
-
bioRxiv - Bioengineering 2023Quote: ... Medium was refreshed every 2–3 days and cells were passaged twice per week using an enzyme-free reagent (ReLeSR, Stem Cell Technologies).
-
bioRxiv - Cancer Biology 2023Quote: ... Whole blood was diluted 1:1 with phosphate-buffered saline (PBS) containing 2% FBS and then layered onto Lymphoprep (STEMCELL Technologies, 07851) in SepMate-50 isolation tubes (STEMCELL Technologies ...
-
bioRxiv - Immunology 2023Quote: ... Primary B cells were isolated from spleens of 2–3 month old C57Bl/6N mice by negative selection with EasySep™ Mouse B Cell Isolation Kit (# 19854, STEMCELL Technologies).
-
bioRxiv - Cell Biology 2024Quote: ... cells were treated with cardiomyocytes differentiation basic medium (2 mM L-glutamine RPMI-1640 plus 1x B27 minus insulin) containing 5 μM CHIR99021 (STEMCELL Technologies, Canada) for 48 hours ...
-
bioRxiv - Immunology 2024Quote: B cells were isolated from 2 healthy PBMC donors using the EasySep™ Human B-cell Isolation Kit (Catalog #17954) from StemCell Technologies. Cells were then stained with APC anti-IgD-APC ...
-
bioRxiv - Bioengineering 2024Quote: ... Fresh gonadal and perirenal adipose tissues were minced with scissors and incubated in collagenase type II (2 mg/mL; StemCell Technologies, CA) and DNase I (20 µg/mL ...
-
bioRxiv - Biophysics 2024Quote: ... Labeled ASCs were suspended in 2 mL PBS and incubated for 5 minutes in an EasySep™ magnet (Stemcell Technologies, Vancouver, CAN) to separate the iron-labeled cells from the unlabeled.
-
bioRxiv - Bioengineering 2024Quote: ... Purified cell populations were expanded in endothelium maintenance media consisting of conditioned media diluted at 1:1 ratio with StemPro-34 SFM supplemented with 2 µg/mL heparin (STEMCELL Technologies, 07980). Media was replaced every other day or until the conditioned media was depleted ...
-
bioRxiv - Cancer Biology 2024Quote: Primary cells were seeded in 12-well plate at a density of 1×106 cells per well in 2 ml StemSpan™SFEM II media (STEMCELL Technologies) with growth factors cocktail (100 ng/ml SCF ...
-
bioRxiv - Developmental Biology 2021Quote: Cells were plated at 2 days after cell sorting (Day 6 post differentiation) into a methylcellulose-based medium containing hematopoietic cytokines (MethoCult M3434, StemCell Technologies, Cat. 03434) at 6000 cells/ml ...
-
bioRxiv - Cell Biology 2021Quote: ... 5 × 105 cells were pelleted at 350x g for 2 min and transferred onto a 6-well transwell membrane (Stem Cell Technologies, #3450). Pellets were incubated with CHIR99021 (5 µM ...
-
bioRxiv - Molecular Biology 2021Quote: ... Cells were resuspended in PBS+2% FBS and then processed for FACS staining or frozen in 10% Cryostor (Stem Cell Technologies, Vancouver, BC). For intracellular staining ...
-
bioRxiv - Molecular Biology 2021Quote: ... Cells were resuspended in PBS+2% FBS and then processed for FACS staining or frozen in 10% Cryostor (Stem Cell Technologies, Vancouver, BC).
-
bioRxiv - Bioengineering 2021Quote: ... T cells were frozen at 1-2 × 107 cells/mL and NK cells were frozen at 5 × 106 cells/mL in CryoStor CS10 (STEMCELL Technologies, Cambridge, MA) and thawed into culture as needed ...
-
bioRxiv - Cell Biology 2022Quote: ... These cells were frozen as a master cell bank (passage 1) and working cell bank (passage 2) in CryoStor CS10 freezer medium (STEMCELL technologies, Vancouver, Canada), supplemented with Y-27632 (5 μM ...
-
bioRxiv - Genomics 2024Quote: ... hESCs were fed every day for mTeSR1 or every 2 days for mTeSR Plus and passaged every 5–6 days using ReLeSR (Stem Cell Technologies 05872).
-
bioRxiv - Neuroscience 2024Quote: ... with a tet-on SOX9 and NFIA overexpression vector were grown to 30% confluency and then treated with small molecules SB43152 (STEMCELL Technologies; 2 µM), DMH1 (STEMCELL Technologies ...
-
bioRxiv - Genomics 2024Quote: ... HPCs were plated at 22,000 cells/cm2 onto a Matrigel-coated 6-well plate containing 2ml of Microglia differentiation medium (Basal Medium with supplement 1 and 2 at 1:9 and 1:225, respectively; STEMCELL Technologies, #100-0019). Cells were supplemented with fresh half-medium every other day from day 0 to day 10 ...
-
bioRxiv - Cancer Biology 2024Quote: ... containing 100 U ml−1 IL-2 in the presence of ImmunoCult™ CD3/CD28/CD2 T cell activator (Stem Cell Technologies # 10970) in 96 well plates overnight (150 ...
-
bioRxiv - Cancer Biology 2024Quote: ... supplemented with 20ng/mL recombinant human IL-2 and 7μL/mL of ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator (Stem Cell Technologies, 10970) every week.
-
bioRxiv - Neuroscience 2022Quote: ... medium was refreshed every 7 days by replacing half of the medium with Brainphys neuronal medium supplemented with 2% NeuroCult SM1 neuronal supplement (Stem cell technologies, Köln, Germany) and 1% pen/strep (BP-complete medium).
-
bioRxiv - Developmental Biology 2021Quote: E14 and its derivative reporter lines pMsgn1-Venus [1] and Hes7-Achilles [2] mouse ESCs were maintained under feeder-free conditions in gelatin-coated dishes (StemCell Technologies cat. no. 07903) with 2i medium composed of high glucose DMEM (Gibco cat ...