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Citations for Stemcell Technologies :
451 - 500 of 1366 citations for 2 1H Imidazol 1 yl 6 methyl 4 pyrimidinemethanamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... Appropriate numbers of total bone marrow cells were then exposed to various concentration of MMC and seeded into 6-well plates with MethoCult GF M3434 (Stem Cell Technologies) media following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: ... were harvested from 6-12 weeks old in-house bred BL/6 mice (WT or transgenic CD2 Cre/CD4 Cre mice) using the EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (Stem Cell Technologies). HSPCs were transduced with retrovirus for expression of the desired oncogenes ...
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bioRxiv - Developmental Biology 2022Quote: Each interstitial cell fraction in in vitro culture on day 3 or day 6 was digested with Accumax™ (STEMCELL Technologies #07921) for 5 min at 37 °C ...
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bioRxiv - Immunology 2022Quote: Untouched PMNs were isolated from bone marrow in donor mice of the indicated genotype (age 6–8 wk) using EasySep mouse neutrophil enrichment kit (Stemcell Technologies Inc.). In a subset of experiment PMNs from C57/J animals were incubated with 1 mM Dimethyloxalylglycine (DMOG ...
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bioRxiv - Cell Biology 2021Quote: ... 288 mio NPCs per axis were aggregated into 14400 organoids (20000 cells/per organoid) in Aggrewell800 6-well plates (Stem Cell Technologies). The day after ...
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bioRxiv - Immunology 2020Quote: ... C57BL/6 mice by use of magnetic separation with the EasySep™ Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (STEMCELL Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... a piece of frozen tumor tissue was placed into a well of a 6-well plate (Stem cell Technologies, catalog no. 38015) with 1 mL of either CST ...
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bioRxiv - Bioengineering 2021Quote: ... 1.5×10^6 cells were used for each targeting and were plated onto geltrex coated wells on 6-well plates in mTeSR with CloneR (Stemcell Technologies, 05889) following nucleofection ...
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bioRxiv - Genetics 2020Quote: ... emerging iPSC colonies that were large enough to isolate were manually picked using a sterile pulled glass pipette into VTN-coated 6-well tissue culture plates containing complete TeSR-E8 media (TeSR-E8 basal medium with 25X TeSR-E8 supplement) (Stem Cell Technologies) using a SMZ1000 Nikon dissecting microscope (Nikon ...
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bioRxiv - Immunology 2023Quote: ... BLaER1 cells were trans-differentiated into Mo for 6-7 days in cRPMI medium containing 10 ng/mL of hrIL-3 (Stem Cell Technologies), 10 ng/mL hrM-CSF (Stem Cell Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... hiPSCs were maintained on a Corning Matrigel (0.5 mg/ 6-well plate, dissolved in DMEM/F- 12 medium) cultured in mTeSR1 medium (StemCell Technologies, Vancouver, BC). Cells were passaged every 4 days using Versene solution (ThermoFisher ...
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bioRxiv - Neuroscience 2022Quote: ... the iPSCs were seeded in 6-well plates coated in phenol-red free Matrigel with hematopoietic differentiation media (StemCell Technologies, cat.no. 05310). The media was changed following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... hESC colonies were dissociated into single cells and seeded onto matrigel coated plates at a density of 400-600k cells per well of a 6 well plate in mTeSR (Stem Cell Technologies) containing 10 µM Rock inhibitor (Y27632 ...
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bioRxiv - Cancer Biology 2023Quote: ... Naïve CD8+ T-cells were isolated from the thawed CD14-population on Day 6 using the EasySep Human Naïve CD8+ T Cell Isolation Kit (STEMCELL Technologies Cat. #19258) as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... 5.8 × 105 cells in N2/B27 media and supplements were seeded into one well of a 6 well AggreWell™400 culture plate (STEMCELL Technologies) which was coated with 5% Pluronic acid F-127 (Sigma-Aldrich P2443 ...
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bioRxiv - Genomics 2023Quote: ... single hESC cells were plated at a density of approximately 5×105 per each well of a 6 well plate in the presence of ROCK inhibitor and exposed to commercially available mesoderm induction media (Stemcell Technologies 05221).
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bioRxiv - Cancer Biology 2023Quote: ... 3-month-old and 18-month-old WT C57BL/6 mice were lineage depleted using Mammary Epithelium Enrichment Kit (Stem Cell Technologies) and resuspended in injection media (HBSS +2% FBS+1% PSA+50% Matrigel ...
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bioRxiv - Immunology 2023Quote: CD63+ DCs or CD63- control DCs isolated from the LNs of Balb/c mice were sorted by FACS as described previously and cultured with naïve CD4+ T cells magnetically isolated from Fox3-GFP+ mice (C57Bl/6 background) using EasySep Mouse Naïve Cd4+ T cell isolation kit (Stemcell Technologies, Cat# 19765) at a ratio of 1:5 DC:T cell for 5 days in RPMI containing 10% FBS ...
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Autophagy suppression in DNA damaged cells occurs through a newly identified p53-proteasome-LC3 axisbioRxiv - Cell Biology 2024Quote: Intestinal organoid culture was established from 20 to 25-week-old female C57BL/6 mice by harvesting intestinal epithelial crypts using a protocol published by Stem Cell Technologies for use with their mouse IntestiCult system ...
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bioRxiv - Genomics 2024Quote: ... we seeded thawed iPSC at passage 12 (P12) or above in Matrigel-coated 6-well plates in mTESR1 media (STEMCELL Technologies (SCT), Vancouver ...
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bioRxiv - Neuroscience 2024Quote: ... gene targeted hPSCs were plated at a density of 250 cells per well of a 6-well plate in mTeSR1 medium supplemented with 1X CloneR (STEMCELL technologies, 05888) and incubated for 48 hours ...
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bioRxiv - Cancer Biology 2024Quote: ... femurs and tibias of C57BL/6 (WT) and Jak/2E/B6 Stella Cre mice using EasySep™ Mouse Hematopoietic Progenitor Cell Isolation Kit (STEMCELL Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2024Quote: ... was maintained in an iMatrix-511 silk (Elixirgen Scientific; NI511) coated 6-well plate using mTeSRTM Plus medium (Stemcell Technologies; 100-0276). The differentiation of iPSCs into EPCs was performed using a previously published protocol15 ...
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bioRxiv - Cell Biology 2024Quote: ... 500uL of the cell aggregate suspension were transferred to one well of a 6-well plate previously rinsed with Anti- Adherence Rinsing Solution (STEMCELL Technologies, 07010) containing 2.5mL of N2B27 media ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were plated at 8 × 104 cells per well on a Matrigel-coated 6-well plate in mTeSR1 supplemented 10 µM Y27632 (STEMCELL Technologies, 72307). The following day ...
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bioRxiv - Cancer Biology 2020Quote: ... Pellet is resuspended in 2-5ml accutase™ (StemCell Technologies, 07920), depending on the sample amount ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were collected with 2 mL mTeSRplus (Stem Cell Technologies, 05825), span down and resuspended in 1 mL mTeSRplus ...
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bioRxiv - Cancer Biology 2020Quote: ... Pellet is resuspended in 2-5ml accutase™ (StemCell Technologies, 07920), depending on the sample amount ...
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bioRxiv - Bioengineering 2021Quote: ... additionally supplemented with cytokines (Human Recombinant IL-2; Stemcell Technologies - #78036). To stimulate the cells ...
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bioRxiv - Cancer Biology 2022Quote: ... PHA was washed and IL-2 (50 U/ml, StemCell Technology) was added to the medium and replaced every 2 days for a time period of 5 days.
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A scalable, GMP-compatible, autologous organotypic cell therapy for Dystrophic Epidermolysis BullosabioRxiv - Bioengineering 2023Quote: ... 2% BSA (Miltenyi) and 10 uM ROCK inhibitor (StemCell technologies 72302). For all wash steps ...
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bioRxiv - Immunology 2024Quote: ... and 50 IU/mL of IL-2 (STEMCELL Technologies, cat. 78220) at 1 million cells/mL ...
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bioRxiv - Microbiology 2024Quote: ... then grown in presence of 100U/mL IL-2 (StemCell Technologies) for 4 days before being used in the various experiments ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 2% Neurocult SM1 Neuronal Supplement (StemCell Technologies; cat. #05711), 1% horse serum (Atlanta Biologicals ...
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bioRxiv - Neuroscience 2024Quote: ... coated plates with 1.5-2 ml of mTeSR1 media (STEMCELL Technologies), media were changed daily except the day after passing ...
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bioRxiv - Cell Biology 2024Quote: ... supplemented with or without 10 µM IWP-2 (STEMCELL Technologies, #72122) and 5% Matrigel matrix ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 300IU/ml recombinant human IL-2 (Stemcell Technologies, 78036) for 14 days ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were passaged every 4 to 7 days with ReLeSR reagent (StemCell Technologies catalog #05872) to ensure that only undifferentiated stem cells remained in the cultures ...
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bioRxiv - Genomics 2021Quote: ... and 4) one cord blood (CB001) collected with informed consent by StemCell Technologies (catalog #70007) (Table S1) ...
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bioRxiv - Cancer Biology 2023Quote: Primary cells are resuspended in a 3:4 solution of Mammocult medium (Stem Cell Technology) and Matrigel (Corning)17,26,29 ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were passaged every 4 to 5 days using ReLeSR (StemCell Technologies, catalog no. 05872). We performed characterization of our iPSCs by immunofluorescence ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were passaged every 3-4 days by dissociating them using Accutase (STEMCELL Technologies) according to manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... and passaged every 3-4 days using Gentle Cell Dissociation Reagent (STEMCELL Technologies, 100-0485) for clump passaging ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were then treated with Wnt pathway inhibitor IWP-4 (5 µM) (Stemcell Technologies, #72552) for 48 hours followed by CM(- ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were then treated with Wnt pathway inhibitor IWP-4 (5 μM) (Stemcell Technologies, #72552) for 48 hours followed by CM(- ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were passaged every 4 days by non-enzymatic dissociation with ReLeSR (StemCell Technologies Inc.) and plated at a density of 5.0×105 per 60 mm plate.
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bioRxiv - Cancer Biology 2024Quote: 200-500 edited residual HSCs were added to 4 mL of MethoCult H4435 (Stemcell Technologies) and differentiated for 14 days ...
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bioRxiv - Genetics 2024Quote: ... every other day and passaged every 3–4 days with Accutase (Stem Cell Technologies 07920) or ReLeSR (Stem Cell Technologies 100-0483) ...
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bioRxiv - Developmental Biology 2022Quote: ... and 20 ng/ml BMP4 supplemented with 10 μM Y-27632 in each well of 6-well AggreWell 400 plates (Stem Cell Technology, 34415). Next day the formed cell spheroids clusters were washed with RPMI-1640 medium with 1x glutamax ...
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bioRxiv - Cancer Biology 2020Quote: ... The cells were then seeded in triplicates at a concentration of 100,000 cells/well into 6-well culture plates with M3434 methylcellulose (Methocult, Stem Cell Technologies, Vancouver, Canada). For assessing the differentiation pattern of naïve bone marrow cells and MDPs in the presence of tumor-derived conditioned medium (TCM) ...