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Citations for Harvard Apparatus :
51 - 100 of 101 citations for Lysophosphatidic Acid Receptor 5 LPAR5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Whole-cell current-clamp recordings were made from pyramidal cells in area CA1 of the hippocampus using patch pipettes (5– MΩ) manufactured from thick-walled glass (Harvard Apparatus). Pyramidal cells were identified by their position in the slice ...
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bioRxiv - Neuroscience 2019Quote: ... Briefly, mice were anaesthetised with isoflurane (5% induction, 1.5-2% maintenance) and secured in a stereotaxic frame (Harvard Apparatus, Massachusetts, U.S.A). A small craniotomy was performed and up to 700nL of the viral sample was injected using a picospritzer (PV820 ...
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bioRxiv - Neuroscience 2020Quote: ... and a 33-gauge bilateral injector (cut length 5 mm; Plastic One) attached to a syringe pump (PHD ULTRA; Harvard Apparatus) was lowered into the guide cannula previously implanted over the MFB (depth −4.8 mm from brain surface) ...
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bioRxiv - Neuroscience 2021Quote: ... Recording electrodes with impedance of 3-5 MΩ were pulled from borosilicate glass capillaries (Harvard Apparatus, Kent, UK; 1.5 mm OD) using a micropipette electrode puller (DMZ Universal Puller) ...
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bioRxiv - Neuroscience 2020Quote: ... ASV infusion was carried out at 0.125 μl/min using an injection cannula extending out of PE50 tubing attached to a 5 μl Hamilton syringe (Hamilton) using a PHD 2000 Infusion Pump (Harvard Apparatus). After injection ...
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bioRxiv - Developmental Biology 2019Quote: ... Cerebral organoids were subsequently electroporated with 5 pulses applied at 80V for 50ms each at intervals of 500ms (ECM830, Harvard Apparatus). Following electroporation ...
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bioRxiv - Neuroscience 2021Quote: ... 350 nl at 700 nm depth and 350 nl at 400 nm depth) using a Hamilton syringe (5 μl, 32-gauge) and an infuse/withdrawal pump (Harvard Apparatus). pAAV-CamKiia-eNpHR3.0-EYFP was a gift from Karl Deisseroth (Addgene viral prep #26971-AAV1 ...
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bioRxiv - Neuroscience 2021Quote: An optical window (made from two 3 mm glass coverslips and a 5 mm glass coverslip (Harvard Apparatus, Holliston, MA, USA) sealed with optical adhesive (Norland ...
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bioRxiv - Neuroscience 2020Quote: ... Briefly, mice were anaesthetised with isoflurane (5% induction, 1.5-2% maintenance) and secured in a stereotaxic frame (Harvard Apparatus, Massachusetts, U.S.A). Two small craniotomies were performed and up to 700 nL of viral sample was injected using a picospritzer (PV820 ...
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bioRxiv - Neuroscience 2022Quote: ... Larvae were injected in the left brain hemisphere with 5 nl of nKTP solution using pulled borosilicate glass microcapillary (GC100F-15, Harvard Apparatus) pipettes under a stereomicroscope (MZ10F ...
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bioRxiv - Neuroscience 2023Quote: ... Whole-cell current-clamp recordings were made from pyramidal cells in area CA1 of the hippocampus using patch pipettes (5–10 MΩ) manufactured from thick-walled glass (Harvard Apparatus). Pyramidal cells were identified by their position in the slice ...
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bioRxiv - Neuroscience 2023Quote: ... Current clamp and voltage clamp recordings were performed with pipettes (5- to 7-MΩ resistance) prepared from borosilicate glass (BF150-86-10; Harvard Apparatus) using a DMZ pipette puller (Zeitz) ...
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bioRxiv - Neuroscience 2023Quote: ... Volumes for bilateral HPC infusions were 200 nl/hemisphere (rate = 5 μL/min) via a 33-gauge injector and microsyringe attached to an infusion pump (Harvard Apparatus). Injectors were left in place for 20 s after infusions ...
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bioRxiv - Neuroscience 2023Quote: ... The bowel was removed and placed in warmed (37°C), oxygenated (95% O2, 5% CO2) Krebs-Ringers solution in a horizontal organ bath (Hugo Sachs Elektronik, Harvard Apparatus)35 ...
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bioRxiv - Neuroscience 2023Quote: ... which was attached to a 5 μL Hamilton syringe (Hamilton, Reno, NV) that was placed in an infusion pump (CMA syringe pump 4004; Harvard apparatus). We infused rimonabant (2 µg/µL ...
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bioRxiv - Neuroscience 2023Quote: ... Embryos were electroporated at E15.5 by applying electric pulses (50V; 50ms on/950ms off/5 pulses) with an electric stimulator (BTX Electroporator ECM 830 (Harvard Apparatus)) using round electrodes (CUY650P5 ...
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bioRxiv - Bioengineering 2023Quote: ... The micropipette holder was connected to a 5 ml syringe which was pre-filled with PBS and mounted on a syringe pump (PHD 2000, Harvard Apparatus). The glass tube was inserted into the brain tissue to the targeted coordinates ...
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bioRxiv - Neuroscience 2024Quote: ... bubbled with 95% O2 – 5% CO2. Patch micropipettes (open tip resistance of approx. 6 MΩ) were pulled from borosilicate glass capillaries (Harvard Apparatus), using the Sutter Instruments P97 puller and filled with the solution containing (in mM) ...
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bioRxiv - Biophysics 2024Quote: ... we kept the flow at a rate of 5 ml/min in both source and sink channels with a syringe pump (Pump-22; Harvard Apparatus). The potassium ions could diffuse from the source channel to the sink channel and form a linear gradient in the observing channel ...
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bioRxiv - Microbiology 2022Quote: ... Mice were injected with 0.5 μL of sterile 0.9% NaCl or 2 × 108 CFU/mL M.tb through the brain cannula (over 5 min) using the syringe pump (Harvard Apparatus, Holliston, Massachusetts). Mice in the i.v ...
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bioRxiv - Neuroscience 2020Quote: ... Pressure transducers were connected to a signal conditioner module (EVO-SD-5/FPI-LS-10, FISO Technologies Inc., Harvard Apparatus, Quebec, Canada) and data were continually recorded using the Evolution software (FISO Technologies Inc. ...
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bioRxiv - Immunology 2022Quote: Mice were anesthetized using an induction chamber with an atmosphere saturated with 5% Isoflurane (Cristália®, São Paulo, Brazil) in oxygen (Isoflurane Anesthesia Vaporizer System; Harvard Apparatus, MA, USA). Using a 300 µL 30 G syringe (BD Ultra-FineTM) ...
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bioRxiv - Neuroscience 2019Quote: ... 0.5 pulses at 30 V with 400 ms intervals were applied with a 5 mm tweezer electrode using an electroporator (BTX, Harvard Apparatus, ECM830). Following electroporation ...
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bioRxiv - Developmental Biology 2019Quote: ... Embryos at the one cell stage were injected with 5–10 nL of working solutions using borosilicate glass pipettes (GC100F15, Harvard Apparatus LTD) pulled in a Narishige PN-30 Puller (Japan).
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bioRxiv - Neuroscience 2019Quote: 2-month-old mice were trained on 3 consecutive days with at least 2 trials per day on an accelerating rotarod (4 rpm to 45 rpm over 5 mins, Harvard apparatus, UK). A training trial was considered successful when the mice stayed on the rod for at least 45 sec ...
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bioRxiv - Neuroscience 2019Quote: ... Five voltage pulses (50 V, 50 ms duration, 1 Hz) were delivered using 5 mm round plate electrodes (ECM™ 830 electroporator, Harvard Apparatus), placing anode or cathode on the top of the skull to target cortex or hippocampus ...
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bioRxiv - Neuroscience 2021Quote: ... Patch-pipettes with a resistance of 5-7 MΩ were made from borosilicate glass capillary tubes (0.86mm internal diameter; Harvard Apparatus, Cambridge, UK) using an electrode puller (P-87 ...
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bioRxiv - Physiology 2021Quote: ... and animals were ventilated with an inspiration gas mixture (21 % O2, 5% CO2, and N2 balanced) via a rodent ventilator (Respirator 645, Harvard Apparatus, USA). The rats were continuously maintained at 85 breaths/min and tidal volume at 10 ml/kg ...
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bioRxiv - Bioengineering 2021Quote: ... 3 μM and 5 μM melittin prepared in saline was infused through the ICA catheter by a syringe pump (11 PLUS, Harvard Apparatus Inc.). For each animal ...
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bioRxiv - Physiology 2023Quote: ... a positive end-expiratory pressure (PEEP) of 5 cmH2O and a respiratory rate of 120 breaths/min (VentElite, Harvard Apparatus, Holliston, MA). Following a 10-min stable baseline period ...
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bioRxiv - Physiology 2023Quote: The treadmill test was performed with reference to the protocol used for the running test using 5 LANE TREADMILL for MICE (Harvard Apparatus, USA) [20 ...
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bioRxiv - Neuroscience 2023Quote: ... or PBS (control hemisphere) were injected over 50 min (5 µl/min) using a Harvard microinjector (Harvard Pump 11 Elite, Harvard Apparatus, USA), the slow rate being necessary to allow for diffusion in the cerebral parenchyma and to avoid significant backflow ...
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bioRxiv - Neuroscience 2020Quote: ... Agar slices containing ventral forebrain tissue were placed onto a positive genepaddles electrode (5×7mm; Harvard Apparatus Inc #45-0123; Holliston, MA, USA) from a BTX ECM 830 squarewave electroporation system under a stereo microscope ...
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bioRxiv - Physiology 2020Quote: ... Whole-cell current- and voltage-clamp recordings were made from pyramidal cells in area CA1 of the hippocampus using patch pipettes (5–10 MΩ) manufactured from thick walled glass (Harvard Apparatus, Edenbridge UK) and containing (mM) ...
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bioRxiv - Cancer Biology 2020Quote: ... 106 cells expressing a PLX304-mCherry-LUC vector (5 μl cell suspension) were implanted using a guide screw and a multiport microinfusion syringe pump (Harvard Apparatus, Holliston, MA) (Lal et al. ...
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bioRxiv - Neuroscience 2021Quote: ... Solutions were infused at a rate of 0.2 µL/min using a 5 µL Hamilton syringe (Hamilton, Bonaduz, Switzerland) and an automated microsyringe pump (Harvard Apparatus, Holliston, MA, USA). To allow for the diffusion of AAVs into the tissue ...
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bioRxiv - Neuroscience 2019Quote: ... Drugs were delivered into CeA with a 5 μl syringe (Hamilton, model 95N) connected to a polyethylene tubing (PE-10) with an infusion pump (Harvard Apparatus - PHD 2000), 200 nl of SAL or 200 nl of OT ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The injectors were connected via tubing to 5-µL syringes placed on a microsyringe pump (HARVARD PHD, 2000: HARVARD Apparatus, Saint-Laurent, Canada). Following infusion ...
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bioRxiv - Neuroscience 2023Quote: ... fEPSPs were evoked via alternating test stimulation (50µs, 5-20V) delivered through bipolar electrodes (Hugo Sachs Elektronik-Harvard Apparatus, Germany; 50µm tip diameter) placed on both sides of the recording pipette ...
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bioRxiv - Neuroscience 2022Quote: ... Whole-cell recordings were made from pyramidal cells in area CA1 of the hippocampus using patch pipettes (5–10 MΩ) manufactured from thick walled glass (Harvard Apparatus, Edenbridge UK) and containing (mM) ...
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bioRxiv - Neuroscience 2023Quote: ... 1 µL of the OXTR antagonist OTA (0.5 mg/mL) or equal volume of saline was infused over 2 min with Pump 11 Elite Syringe Pump (Harvard Apparatus, Item# HA1100). Pup retrieval test started 5 min following infusion ...
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bioRxiv - Cell Biology 2023Quote: ... and RNPs were electroporated with a single pulse of 400V for 5 msec using the BTX ECM 830 Square Wave Electroporation System (BTX/Harvard Apparatus, Holliston, MA). Electroporated CD34+ cells were placed back into the same culture media overnight before differentiation cultures ...
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bioRxiv - Molecular Biology 2023Quote: ... and mixed with 10 µl of linearized DNA (5-15 µg) in a 0.2 cm electroporation cuvette (BTX, Harvard Apparatus, Holliston, MA, USA). Parasites were then subjected to one pulse using X-001 program in the Amaxa Nucleofector 2b (Lonza Cologne AG ...
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bioRxiv - Neuroscience 2020Quote: Locomotors coordination was evaluated during 4 successive session-tests performed at a luminosity of 115 +/- 15 lux on a rotarod system composed of a rotating plastic bar covered by grey rubber foam (5 cm in diameter, Panlab Harvard apparatus LE 8200, Bioseb). The animals were first habituated to stay on the rod for 30 s at a constant speed of 4 rotations per minute (rpm) ...
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bioRxiv - Neuroscience 2020Quote: ... The test was performed at a luminosity of 70 +/- 5 lux in the plexiglass square arena used for open field test (44 x 44cm x 16 cm, Panlab Harvard apparatus IR ACTIMETER, Bioseb), considered as a habituation session 24h before training session ...
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bioRxiv - Neuroscience 2021Quote: ... was infused over 1 min through injectors connected to 5-μL Hamilton syringes (7000 series, Hamilton Co., Reno, Nevada) controlled by a microsyringe pump (Harvard PhD 2000, Harvard Apparatus, Saint-Laurent, Canada). Injectors were left in place for 1 additional minute to allow diffusion ...
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bioRxiv - Neuroscience 2020Quote: ... Each pair of infusion cannula was connected to two 5 μl Hamilton syringes (Bonaduz, Switzerland) mounted on two infusions pumps (Harvard Apparatus Ltd, Kent, UK). A volume of 0.25 μl per hemisphere was infused over 1 minute ...
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bioRxiv - Neuroscience 2023Quote: ... Transplantation was stereotaxically performed for each mouse bilaterally through drilled holes in the skull using a 5-μl Hamilton syringe with 32-gauge needle and an injecting minipump (Nanomite Injector Syringe Pump; Harvard Apparatus, Holliston, MA, USA). A volume of 2 μl of cell suspension was injected at a rate of 0.5 μl/min at the following coordinates (from bregma and brain surface) ...
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bioRxiv - Neuroscience 2024Quote: ... was placed in the OFC (AP: +3; ML: +2; DV: -5, in mm from bregma and dura) and a glass microelectrode (PG150-T, Harvard Apparatus, Holliston, MA, USA) filled with 0.4 M NaCl solution was lowered in the DMS (AP ...
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bioRxiv - Neuroscience 2022Quote: To measure the response of sDarken to exogenous serotonin 10 μM 5-HT was applied using a customized borosilicate glass pipette (Harvard Apparatus, ~ 2-4 μm diameter). To image the response of sDarken to endogenous serotonin release ...