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Citations for Harvard Apparatus :
101 - 150 of 234 citations for 6 1 2 4 TRIAZOL 1YL PURINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... 47.5 % O2 (0.6 l/ min) and 47.5 % N2O (0.4 l/ min) and maintained with 2 % isoflurane (Harvard Apparatus anesthetic vaporizer ...
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bioRxiv - Cancer Biology 2022Quote: ... were microinjected into yolk sac of 2-day old embryos using a pressure injector (Harvard Apparatus, Holliston, MA) and manipulator (MM33-Righ ...
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bioRxiv - Biophysics 2023Quote: ... The three solutions were flow stratified for a minimum of 2 min using a syringe pump (Harvard Apparatus) with flow rates controlled by syringe size to maintain stream width ratios of 4:1:4 ...
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bioRxiv - Molecular Biology 2023Quote: ... 6e6 cells were then electroporated in a 2 mm path length electroporation cuvette (BTX Harvard Apparatus; Holliston, MA) with 5μg (HCV ...
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bioRxiv - Neuroscience 2021Quote: Mice were anesthetized using 4% isoflurane and placed in a stereotaxic frame (Kopf Instruments, Model 900) equipped with a digital micromanipulator (Harvard Apparatus, Holliston, MA). Anesthesia was maintained with 0.5-2% isoflurane and/or a cocktail of ketamine/xylazine (100/7 mg/kg i.p.) ...
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bioRxiv - Microbiology 2023Quote: ... The mixture was then electroporated at 2 kV and 50 ohm resistance using a BTX Gemini X2 (Harvard Apparatus). Next ...
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bioRxiv - Neuroscience 2022Quote: ... Patch pipettes (resistance: 2-8 MΩ) were prepared from borosilicate glass capillaries (1.5 outer diameter x 0.86 inner diameter; Harvard Apparatus) using a Flaming/Brown micropipette puller (P-97 ...
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bioRxiv - Neuroscience 2023Quote: Male and female mice between 2 and 10 months-old were anesthetized with isofluorane (Harvard Apparatus, Iso-Vet Chanelle) mixed with O2-enriched air (induction ...
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bioRxiv - Developmental Biology 2022Quote: ... fertilised eggs were dejellied one hour after fertilisation as above and transferred to 2% Ficoll (Pharmacia)/0.3x MMR and injected with 2-10 nl of solution as desired using an air-driven injector (Harvard Apparatus). Injected embryos were washed into 0.1x MMR for several hours-to-overnight after injection.
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bioRxiv - Neuroscience 2022Quote: ... 47.5 % O2 (0.6 l/ min) and 47.5 % N2O (0.4 l/ min) and maintained with 2 % isoflurane (Harvard Apparatus anesthetic vaporizer). A standard craniotomy (27 ...
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bioRxiv - Neuroscience 2022Quote: ... and maintained for the rest of the surgery under anesthesia (isoflurane, 1.5-2%) and 37°C body temperature (closed-loop heating pad system, Harvard Apparatus). Animals were administered antibiotics (Cefazolin ...
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bioRxiv - Neuroscience 2024Quote: ... During the rest of the surgery, anesthesia (isoflurane, 1.5-2%) and a 37C body temperature (closed-loop heating pad system, Harvard Apparatus) were maintained ...
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bioRxiv - Molecular Biology 2023Quote: ... was introduced into the flow cell in TB buffer at a flow rate of 4 μl/min using a syringe pump (Harvard Apparatus, Pico Plus Elite 11). The flow cell was then washed again with 40 μl TB buffer at a flow rate of 4 μl/min ...
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bioRxiv - Biophysics 2021Quote: ... The excess BSA was washed out of the chamber with 200 µL T50M5C2 buffer at a flow rate of 2 mL/hr controlled by a syringe pump (Harvard apparatus). All wash steps were performed in this manner ...
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bioRxiv - Neuroscience 2020Quote: ... 2 × 109 genome copies (gc) of AAV2/5 GfaABC1D-mito7-GCaMP6f was injected in a 2 μL volume using a syringe pump (Harvard Apparatus). The injection pipette was left in place for ~10 min after injection and then gradually withdrawn ...
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bioRxiv - Neuroscience 2019Quote: ... Briefly, mice were anaesthetised with isoflurane (5% induction, 1.5-2% maintenance) and secured in a stereotaxic frame (Harvard Apparatus, Massachusetts, U.S.A). A small craniotomy was performed and up to 700nL of the viral sample was injected using a picospritzer (PV820 ...
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bioRxiv - Neuroscience 2020Quote: ... A&M systems) m above the tip of the recording electrode (tip diameter: 2-3 μm, impedance: 15-20 MΩ, Harvard Apparatus). The pipette was lowered into the LC ...
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bioRxiv - Neuroscience 2019Quote: ... aCSF was dialyzed at a rate of 2 µL/min by a syringe pump located outside of the chamber (PHD 1000, Harvard Apparatus). After being hooked-up ...
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bioRxiv - Neuroscience 2019Quote: ... The drug and balancing barrels were connected via silver/silver chloride wires to microiontophoresis constant current generators (Harvard Apparatus, BH-2) that were used to generate and monitor retention currents (20 nA ...
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bioRxiv - Neuroscience 2020Quote: ... The drugs were retained in the pipette with a −20 nA current and ejected using 90 nA currents (Neurophore BH-2 system, Harvard Apparatus). Thus ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were placed in a submerged chamber perfused at 2 ml/min with ACSF at 32 °C using an in- line heater (Harvard Apparatus).
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bioRxiv - Physiology 2019Quote: ... Total energy expenditure and respiratory quotient were calculated as the average values of the last 2-h of measurements (Metabolism software version 1.2, Panlab Harvard Apparatus, Spain) which is based on standard methods (29) ...
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bioRxiv - Neuroscience 2020Quote: ... Briefly, mice were anaesthetised with isoflurane (5% induction, 1.5-2% maintenance) and secured in a stereotaxic frame (Harvard Apparatus, Massachusetts, U.S.A). Two small craniotomies were performed and up to 700 nL of viral sample was injected using a picospritzer (PV820 ...
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bioRxiv - Neuroscience 2022Quote: Sample holders for fluorescent beads were built by gluing smaller coverslips (3 mm ⌀, CS-3R-0, Warner Instruments) to a 2-mm glass capillary tube (Harvard Apparatus) using silicone adhesive (Wuerth Super RTV-Silikon) ...
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bioRxiv - Biophysics 2023Quote: ... a solution of 500 μM glycine betaine in buffer C was added in steps of 2 or 10 μL using a Hamilton syringe pump (Harvard apparatus). Excitation and emission wavelengths of 295 and 360 nm (and slit widths of 1 and 5 nm) ...
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bioRxiv - Neuroscience 2023Quote: ... The firing activity of LC neurons was recorded using a recording electrode (tip diameter: 2-3 μm, impedance: 15-20 MΩ, Harvard Apparatus), which was lowered into the LC ...
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bioRxiv - Neuroscience 2023Quote: ... The back end of the water supply line was connected to two solenoid valves (EV-2-12, Clippard) and a syringe pump (PHD2000, Harvard Apparatus) to control the amount and timing of water reward ...
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bioRxiv - Neuroscience 2023Quote: Mice were sacrificed 24 or 72 h after tMCAO and brains were removed and cut into three 2 mm-thick slices (mouse brain slice matrix; Harvard Apparatus). For determination of infarct volume ...
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bioRxiv - Neuroscience 2024Quote: ... balance N2 (air control) – was delivered at a flow rate of 2 mL/min using a syringe pump (PHD 2000, Harvard Apparatus). Video-recording started immediately after the droplet dried and animals started crawling on the agar surface ...
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bioRxiv - Neuroscience 2024Quote: PNKD-Tg mice (N= 9) and their WT littermates (N= 7) were implanted with a microdialysis probe cannula (CMA7/2 mm, Harvard Apparatus) above the dorsal striatum ...
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bioRxiv - Microbiology 2022Quote: ... Mice were injected with 0.5 μL of sterile 0.9% NaCl or 2 × 108 CFU/mL M.tb through the brain cannula (over 5 min) using the syringe pump (Harvard Apparatus, Holliston, Massachusetts). Mice in the i.v ...
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bioRxiv - Neuroscience 2020Quote: ... brains were quickly removed and cut into four 2-mm thick coronal sections using a mouse brain slice matrix (Harvard Apparatus, Spain). Sections were stained for 15 min at room temperature with 2% 2,3,5-triphenyltetrazolium chloride (TTC ...
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bioRxiv - Neuroscience 2020Quote: ... Rectal temperature was maintained at 37.0±0.5 °C during the entire surgical procedure by a rectal temperature probe and a heating pad (Product # TCAT-2 Temperature Controller, Harvard Apparatus, USA). The ventral neck region’s hair was shaved to expose the skin and then disinfected ...
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bioRxiv - Neuroscience 2022Quote: ... Body temperature was monitored throughout the procedure using a rectal probe and maintained at 37 ± 2°C with a heating pad (Harvard Apparatus®). A unilateral craniotomy was performed over the right parietal cortex ...
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bioRxiv - Biochemistry 2019Quote: ... and brains were quickly removed and cut into 2-mm thick coronal sections using a mouse brain slice matrix (Harvard Apparatus, USA). The brain sections were then stained with 2% 2 ...
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bioRxiv - Neuroscience 2020Quote: ... The animals were moved into a warm (37.0 ± 1 °C) recovery chamber (Product #DW-1, Harvard Apparatus, USA) to prevent postischemic hypothermia ...
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bioRxiv - Neuroscience 2020Quote: ... A total volume of 0.6 µL virus diluted 1:1 with sterile artificial cerebrospinal fluid (Harvard Apparatus, Holliston, MA) was infused per side at a rate of 0.15 µL/min ...
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bioRxiv - Physiology 2019Quote: ... Warmed saline (41 °C) was pumped at 2 mL/min (AS50 infusion pump, Baxter, Deerfield, IL; or PHD 2000, Harvard Apparatus, Holliston, MA) through a fluid warmer (Hotline Fluid Warmer ...
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bioRxiv - Neuroscience 2023Quote: ... 1 µL of the OXTR antagonist OTA (0.5 mg/mL) or equal volume of saline was infused over 2 min with Pump 11 Elite Syringe Pump (Harvard Apparatus, Item# HA1100). Pup retrieval test started 5 min following infusion ...
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bioRxiv - Neuroscience 2022Quote: ... and 1 × 1 mm bilateral punches of the CeA were taken using a tissue biopsy punch (Harvard Apparatus, Holliston, MA) prior to storage at -80°C until processing ...
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bioRxiv - Neuroscience 2020Quote: ... When 1 mm membrane probes (CMA8011081; Harvard Apparatus) were inserted ...
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bioRxiv - Neuroscience 2021Quote: ... and a 5mm coverslip number 1 (Harvard Apparatus). Following this the mouse was placed on to the confocal microscope (Leica SPE ...
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bioRxiv - Immunology 2019Quote: ... the muscle was pulsed using an ECM 830 Electro Square Porator™ with 2-Needle Array™ Electrode (BTX Harvard Apparatus®; Holliston, MA) under the following parameters ...
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bioRxiv - Neuroscience 2022Quote: ... C-fiber-evoked field potentials were recorded in the deep lamina of the DH (at a depth range of 250 and 500 µm) with borosilicate glass capillaries (2 MΩ, filled with NaCl 684 mM; Harvard Apparatus, Cambridge, MA, USA). Field potentials were recorded with an ISODAM-amplifier (low filter ...
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bioRxiv - Neuroscience 2021Quote: ... Ibotenic acid or saline was injected into the LH at a rate of 0.25 μL/min for 2 min by using a 26-gauge Hamilton syringe (Reno, NV, USA) and a microinjection pump (Pump 22; Harvard Apparatus, Holliston, MA, USA). The syringe was left in place for at least 5 min to prevent reflux after injection.
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bioRxiv - Cell Biology 2019Quote: ... together with the trachea and the heart were excised from the thorax in one piece and transferred to a 37 °C temperature-equilibrated housing chamber of the perfused mouse lung model (IPL-2, Hugo Sachs Elektronik/Harvard Apparatus (March-Hugstetten, Germany)) ...
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bioRxiv - Neuroscience 2020Quote: ... was injected into the CM at a rate of 2 μL/min via one of the CM cannulae using a syringe pump (Harvard Apparatus Pump 11 Elite). In the second set of experiments ...
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bioRxiv - Neuroscience 2022Quote: ... counted and diluted to 200,000 cells per ml in room temperature growth medium before adding 100 μL of the cell suspension to the wells of a 2 mm electroporation plate (Harvard Apparatus, Holliston, MA, USA) which contained 11 μL of 10X ASO in water ...
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bioRxiv - Neuroscience 2024Quote: ... was placed in the OFC (AP: +3; ML: +2; DV: -5, in mm from bregma and dura) and a glass microelectrode (PG150-T, Harvard Apparatus, Holliston, MA, USA) filled with 0.4 M NaCl solution was lowered in the DMS (AP ...
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bioRxiv - Neuroscience 2019Quote: ... Each mouse was placed nose-down in the center of a wire mesh grid (1 cm × 1 cm) positioned at a 45° angle (Harvard Apparatus). The time taken for a mouse to reorient itself with its nose facing upwards (negative geotaxis ...