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Citations for Harvard Apparatus :
151 - 200 of 224 citations for Cell Isolation Optimizing System since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... we videotaped the harmaline-treated mice’s locomotor activity in the tremor chamber and subsequently analyzed the action/immobile motion of the mice using the SMART Video Tracking System (Harvard Apparatus, MA, USA) (Fig.2B).
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bioRxiv - Molecular Biology 2021Quote: ... were subsequently added and worms were electroporated with a single pulse at 125 V (LV mode) for 20 ms using an ECM 830 Square Wave Electroporation System (BTX, Harvard Apparatus, Holliston, MA). Worms were subsequently transferred to a 48 well tissue culture plate and cultured for up to seven days.
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bioRxiv - Neuroscience 2023Quote: ... Body temperature was maintained at 37.0±0.5°C throughout the procedure with help of a homeothermic monitoring system (Harvard Apparatus 55-7020, Massachusetts, USA). After a midline neck incision ...
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bioRxiv - Cell Biology 2023Quote: ... and RNPs were electroporated with a single pulse of 400V for 5 msec using the BTX ECM 830 Square Wave Electroporation System (BTX/Harvard Apparatus, Holliston, MA). Electroporated CD34+ cells were placed back into the same culture media overnight before differentiation cultures ...
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bioRxiv - Bioengineering 2024Quote: ... The femoral artery was entered with a metal cannula and attached to an intravascular polyethylene tube (Pulmonary artery ball joint system, Harvard Apparatus Holliston, MA) and later secured with a 6-0 polypropylene suture.
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bioRxiv - Neuroscience 2023Quote: ... Five μl of a suspension of U87 cells (100,000 cells/μl) were injected through a Hamilton syringe (1 μl/min, Harvard apparatus, needle pump) into the left frontal lobe through the drilled hole The injection needle was left in place for an additional 5 min after the injection was completed to prevent backflow ...
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bioRxiv - Cancer Biology 2022Quote: ... ID8 cells were electroporated (BTX ECM 830, Harvard Apparatus) with ribonucleoprotein complex and electroporation enhancer (#1075915 ...
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bioRxiv - Microbiology 2022Quote: ... using a BTX Electro Cell Manipulator 600 (Harvard Apparatus). The electroporated cells were promptly transferred by syringe into a sterile ...
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bioRxiv - Neuroscience 2024Quote: ... Whole-cell patch-clamp borosilicate-glass pipets (Harvard Apparatus) were obtained with a horizontal two-stage puller (P-1000 ...
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bioRxiv - Neuroscience 2020Quote: Locomotors coordination was evaluated during 4 successive session-tests performed at a luminosity of 115 +/- 15 lux on a rotarod system composed of a rotating plastic bar covered by grey rubber foam (5 cm in diameter, Panlab Harvard apparatus LE 8200, Bioseb). The animals were first habituated to stay on the rod for 30 s at a constant speed of 4 rotations per minute (rpm) ...
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bioRxiv - Neuroscience 2022Quote: ... Lohmann LSL fertilized chick eggs were incubated until E2.7-E3.0 (HH stages 14-18) and chick embryo neural tubes were electroporated in ovo using the ECM 830 electroporation system (BTX/Harvard Apparatus Inc., Holliston, USA) as described21 ...
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bioRxiv - Neuroscience 2023Quote: ... and aligned on a stereotaxic frame (Kopf Instruments; Tujunga, CA, USA) while on a heated pad (Homeothermic monitoring system, Harvard Apparatus; Holliston, MA, USA). All surgeries were conducted in a sterile environment using aseptic techniques ...
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bioRxiv - Neuroscience 2020Quote: ... cells were aspirated with a thin glass capillary (Harvard Apparatus), previously pulled with a micropipette puller (P-97 ...
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bioRxiv - Neuroscience 2020Quote: ... Whole cell voltage-clamp recordings were performed using pipettes (Harvard Apparatus) made from borosilicate glass capillaries pulled on a Flaming-Brown micropipette puller (Model P-1000 ...
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bioRxiv - Cell Biology 2024Quote: ... Jurkat cells were transfected by electroporation (BTX ECM 830, Harvard Apparatus) as previously described 41 ...
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bioRxiv - Cell Biology 2019Quote: Cells were patched with pipettes made of borosilicate glass (GC150F, Harvard Apparatus) and pulled with a vertical Narishige puller (World Precision Instruments) ...
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bioRxiv - Physiology 2023Quote: ... The cells were patched with borosilicate glass pipettes (Harvard apparatus, Cambourne, UK), with 3 to 7 MΩ resistance when filled ...
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bioRxiv - Microbiology 2020Quote: ... and no resistance using an ECM 630 electro cell manipulator (BTX Harvard Apparatus) into ≈50% confluent T75 flasks of BHK21 cells ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were than electroporated with a pulse generator (ECM830, BTX Harvard Apparatus, Holliston, MA) by applying the following protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... the cells were washed twice in Cytoporation buffer T (BTX Harvard Apparatus, Holliston, Massachusetts), and resuspended at a final concentration of 28□×□106 cells/mL in the same solution ...
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bioRxiv - Biophysics 2020Quote: 5 million cells were electroporated using BTX T820 Electro Square Porator (Harvard Apparatus, Holliston, MA, USA) in 100ul of DPBS with 2.5 ug of plasmid ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... confluent monolayers of ADPKD cells on Snapwell supports were inserted into modified Ussing chambers (Harvard Apparatus), and both surfaces were bathed in a HCO3-Ringer’s solution maintained at 37°C and equilibrated in 5% CO2-95% O2 ...
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bioRxiv - Immunology 2020Quote: ... and then electroporated in Biorad 0.2cm cuvettes using ECM 630 Electro Cell manipulator (BTX Harvard apparatus) at 250V ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were kept at 37°C in a temperature-controlled incubation chamber (Harvard Apparatus, Holliston, MA). Images were acquired with a CoolSNAP HQ2 CCD camera (Photometrics ...
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bioRxiv - Developmental Biology 2022Quote: ... Whole cell recordings were carried out using borosilicate glass electrodes (GC100TF-10; Harvard Apparatus, Edenbridge, UK), fire-polished to resistances of between 16-20 MΩ ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were pulsed once at 130V for 6 mS with the ECM 830 instrument (Harvard Apparatus). After electroporation ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were lifted using 1.5 mm diameter thin wall glass capillary tubes (30-0066 Harvard Apparatus), pulled to a fine tip with a Sutter P-1000 micropipette puller (Sutter Instruments ...
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bioRxiv - Neuroscience 2023Quote: ... Whole-cell current-clamp synaptic responses were recorded using glass electrodes (7-10MΩ; Harvard apparatus, UK), filled with internal solution containing (in mM) ...
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bioRxiv - Neuroscience 2023Quote: ... Fixation media (see above) was applied to cells using a syringe pump (Harvard Apparatus PHD ULTRA) and kept at 37°C using an in-line heater (Warner SH-27B).
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bioRxiv - Immunology 2020Quote: ... cells were injected into 10 animals simultaneously using a multiport Microinfusion Syringe Pump (Harvard Apparatus, Holliston, MA). Animals were anesthetized with xylazine/ketamine during the procedure ...
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bioRxiv - Molecular Biology 2020Quote: ... Two million cells were transferred into 0.2 mm path length electroporation cuvettes (BTX Harvard Apparatus, Hollister, MA) containing 6 µg pCas-BgAIFx4 in ∼100 µl Opti-MEM ...
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bioRxiv - Immunology 2020Quote: ... and then electroporated in Biorad 0.2 cm cuvettes using ECM 630 Electro Cell manipulator (BTX Harvard apparatus) at 250V ...
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bioRxiv - Cancer Biology 2023Quote: ... Cancer cells were loaded into a Borosilicate glass capillary needle (1mm O.D. x 0,78mm I.D., Harvard Apparatus) and injected into the collagen using a microinjector with the PV820 Pneumatic PicoPump (WPI) ...
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bioRxiv - Synthetic Biology 2019Quote: ... the cells were transferred to an ice-chilled 1-mm gap electroporation cuvette (BTX Harvard Apparatus, MA, USA) followed by two consecutive exponential decay pulses with 1.8 kV ...
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bioRxiv - Synthetic Biology 2022Quote: ... the cells were transferred to an ice-chilled 1- mm gap electroporation cuvette (BTX Harvard Apparatus, MA, USA) followed by an exponential decay pulse with 1.8 kV ...
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bioRxiv - Systems Biology 2019Quote: ... We then imaged cells for many hours under constant media infusion via a syringe pump (Harvard Apparatus, MA).
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bioRxiv - Cancer Biology 2023Quote: ... with a concentration of 100,000 cells/ul and loaded in into borosilicate glass capillary needles (1 mm O.D. × 0.78 mm I.D.; Harvard Apparatus). 300-500 cancer cells were injected into duct of cuvier (DoC ...
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bioRxiv - Molecular Biology 2023Quote: ... 6e6 cells were then electroporated in a 2 mm path length electroporation cuvette (BTX Harvard Apparatus; Holliston, MA) with 5μg (HCV ...
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bioRxiv - Genomics 2019Quote: Cells were prepared for immunofluorescence by plating on sterilized 25-mm round coverslips (German borosilicate glass #1.5; Harvard Apparatus) in 6-well tissue culture dishes ...
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bioRxiv - Molecular Biology 2019Quote: A pcDNA-nSMase2 vector (4 pg/cell of DNA) was delivered to OPCs by electroporation (model ECM830, Harvard Apparatus, Holliston ...
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bioRxiv - Biochemistry 2021Quote: ... Inside-out patches were excised from transfected HEK cells using borosilicate glass pipettes (GC150F-15, Harvard Apparatus; Holliston, MA) pulled to a resistance of 1-3 MΩ ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were electroporated with 10 μg of DNA using the BTM-830square waves generator (BTX Instrument Division, Harvard Apparatus). Selection of stable clones was achieved with selective doses of either zeocin (25 μg/mL ...
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bioRxiv - Cell Biology 2020Quote: ... Suspended cells in a glass syringe were introduced into the channel by a syringe pump (Harvard apparatus 11 Elite) at a fixed volumetric flow rate ...
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bioRxiv - Cancer Biology 2021Quote: HEK293T cells were electroporated with 10 μg of DNA using BTM-830 electroporation generator (BTX Instrument Division, Harvard Apparatus). 24 h after transfection ...
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bioRxiv - Immunology 2020Quote: ... 5×106 Jurkat cells with 5μg SLAMF6-CRISPR plasmid were electroporated in Biorad 0.4cm cuvettes using ECM 630 Electro Cell manipulator (BTX Harvard apparatus) at 260V ...
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bioRxiv - Immunology 2020Quote: ... 5×106 T2 cells with 5μg SLAMF6-CRISPR plasmid were electroporated in Biorad 0.4cm cuvettes using ECM 630 Electro Cell manipulator (BTX Harvard apparatus) at 260V ...
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bioRxiv - Microbiology 2024Quote: ... Syringes with the aqueous cell suspensions and with the oil were mounted on two separate syringe pumps (Harvard Apparatus, Pump 11 Elite / Pico Plus Elite OEM Syringe Pump Modules ...
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bioRxiv - Immunology 2019Quote: ... using streptavidin beads followed by fusion with Sp2ab mouse myeloma cells (Abeome, Athens, GA) via electrofusion (Harvard Apparatus, Holliston, MA). Fused cells were incubated at 37°C ...
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bioRxiv - Cell Biology 2021Quote: Embryos were aligned on an agarose plate and injected at the 1-cell stage with RNA or Morpholino diluted in Phenol Red and Danieau’s buffer using a PLI-100 microinjector (Harvard Apparatus). Injection needles were pulled from borosilicate glass capillaries (0.5mm inner diameter ...
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bioRxiv - Immunology 2019Quote: ... and exposed to two DC pulses (1-sec interval) at 1.2 kV/cm for 30 μsec using a BTX Electro Cell Manipulator (Harvard Apparatus). After fusion ...