Labshake search
Citations for Macherey-Nagel GmbH :
351 - 400 of 2221 citations for ssc mir 323 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2023Quote: ... Digestion products were isolated on agarose gels and purified with the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). Plasmid isolation was performed with NucleoSpin Plasmid kit (Macherey-Nagel) ...
-
bioRxiv - Developmental Biology 2021Quote: PCR products were purified using NucleoSpin PCR Clean-up procedure (Macherey-Nagel 740609.250) and mutations were confirmed by sequencing ...
-
bioRxiv - Genetics 2019Quote: ... PCR products were purified using NucleoSpin Gel and PCR Clean-up (Macherey-Nagel), cloned into pGEM®Teasy vector (Promega) ...
-
bioRxiv - Microbiology 2024Quote: PCR products were purified using NucleoSpin Gel and PCR Clean-up (Macherey-Nagel) according to manufacturer’s recommendations ...
-
bioRxiv - Bioengineering 2020Quote: ... Real whitewater was sterilely filtered through 0.2 mm membrane PP filter (Macherey-Nagel) before the experiments ...
-
bioRxiv - Cancer Biology 2019Quote: ... PCR reactions were purified with NucleoSpin® Gel and PCR Clean-up (MACHEREY-NAGEL). Purified reactions were PCR-amplified with primers Illumina_indX_F and Illumina_indX_R (where XXXXXXXX is index ...
-
bioRxiv - Developmental Biology 2021Quote: ... PCR amplicons were purified on NucleoSpin® Gel and PCR Clean- up (Macherey-Nagel). Template DNAs of interest (500 ng each ...
-
bioRxiv - Microbiology 2022Quote: ... overlap PCR products were purified with NucleoSpin Gel and PCR Clean-up (Macherey-Nagel) according to the manufacturer’s instructions and DNA concentration was quantified by using NanoDrop spectrophotometer (A 260 nm).
-
bioRxiv - Microbiology 2023Quote: ... The PCR products were purified with NucleoSpin Gel and PCR Clean-up (Macherey-Nagel) according to the manufacturer’s instructions and DNA concentration was quantified with a NanoDrop spectrophotometer (A260 nm).
-
bioRxiv - Plant Biology 2024Quote: ... PCR products were purified using the NucleoSpin Gel and PCR Clean-up (Macherey-Nagel) and used for in vitro transcription.
-
bioRxiv - Molecular Biology 2021Quote: ... the DNA eluates were de-cross-linked at 65°C overnight with shaking at 900 rpm and purified by NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel), according to the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2022Quote: ... All generated constructs were digested (20 U Dpn1; 2 h, 37 °C) and purified using PCR clean-up kit (Macherey-Nagel). As eGFP-control ...
-
bioRxiv - Systems Biology 2019Quote: ... Histone-DNA complexes were isolated from insoluble nuclear chromatin by centrifugation and DNA was extracted with a NucleoSpin PCR Clean-up kit (Macherey-Nagel). For CUT&RUN quantitative PCR ...
-
bioRxiv - Genomics 2020Quote: ... for 30 min at 37 °C and gel purified with a NucleoSpin Gel and PCR Clean-up Mini kit (Macherey-Nagel). The oligo-pool amplicons were assembled into the linearized Lenti-gRNA-Puro plasmid using NEBuilder HiFi DNA Assembly Master Mix (NEB ...
-
bioRxiv - Immunology 2020Quote: ... The amplified products were identified by gel electrophoresis and purified using Nucleospin PCR and Gel Clean Up Kit (Macherey-Nagel # 740609.5). Alternatively ...
-
bioRxiv - Bioengineering 2019Quote: ... PCR products were analysed by agarose gel electrophoresis and gel purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). Constructs were transformed into E ...
-
bioRxiv - Microbiology 2022Quote: ... the band corresponding to the amplicon was excised from the gel and purified using NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) following manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2021Quote: ... the samples were excised from 2% agarose gels and purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) using NTC buffer for solubilization of the gel slices ...
-
bioRxiv - Microbiology 2021Quote: ... Both PCR products were excised from a 1% agarose gel and extracted using the Nucleospin Gel and PCR clean-up kit (Macherey-Nagel) and used as templates for In-Fusion HD cloning (Takara) ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... PCR products with multiple bands were excised and purified from gel using the Nucleospin Gel and PCR Clean-up kit from Macherey-Nagel, or cloned using the NEB® PCR cloning kit (New Englands BioLabs ...
-
bioRxiv - Biochemistry 2019Quote: ... the extracted ChIP DNA was further re-purified using NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany). The region-specific primer sets used for the ChIP-qPCR analysis have been mentioned in Table S2.
-
bioRxiv - Synthetic Biology 2021Quote: ... the vector pEHLYA5 in the band corresponding to 3612 bp was cleaned with Nucleospin Gel and PCR clean-up kit (Macherey-Nagel). Plasmids pCANTAB6_H107Y ...
-
bioRxiv - Microbiology 2020Quote: ... primers 4163-4183 and 4288-4308) were amplified by PCR (Rotor Gene 3000, Corbett Research) from 50ng DNA (Nucleospin Tissue kit, Macherey-Nagel). PCR products quantified with Sybr green (Thermo Fisher Scientific ...
-
bioRxiv - Plant Biology 2020Quote: ... by electrophoresis at 90 V for 1 h and the 1.5 kb target band was collected for purification using NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel) according to the manufacturer’s instruction prior to sequencing ...
-
bioRxiv - Genomics 2021Quote: ... Resulting amplicons were loaded on a 1% agarose gel and purified using the Nucleospin Gel and PCR clean-up kit (Macherey-Nagel). Libraries were sequenced paired-end 75bp on the NextSeq 500 platform yielding approximately 4*106 fragments per sample (Supplementary Table 2) ...
-
bioRxiv - Genomics 2021Quote: ... by loading 400 ng of each pooled sample on an agarose gel followed by a cleaning step using the Nucleospin Gel and PCR Cleanup kit (Macherey-Nagel). Samples were PCR amplified using the Phusion High-Fidelity DNA Polymerase (New England Biolabs ...
-
bioRxiv - Neuroscience 2021Quote: ... The resulting SORL1-202 amplicons were spin-column purified using a Nucleospin Gel and PCR Clean-up Kit (#740609, Macherey-Nagel) prior to validation by DNA sequencing ...
-
bioRxiv - Genetics 2020Quote: ... DNA was eluted from the beads in Tris-EDTA buffer and cleaned up using the Nucleospin Gel and PCR Purification kit (Macherey-Nagel). For every cell line ...
-
bioRxiv - Developmental Biology 2022Quote: ... The amplicon with the expected size was excised from the agarose gel and purified using the NucleoSpin Gel and PCR clean-up kit (Macherey-Nagel). In order to induce a DNA double-strand break in close proximity to the site of insertion ...
-
bioRxiv - Microbiology 2022Quote: ... Barcoded amplicons were extracted from agarose gel (1.5%, 1 h, 100 V) by using NucleoSpin® Gel and PCR Cleanup XS kit (Macherey-Nagel) and the amplicon size and DNA concentration were checked with High Sensitivity D1000 ScreenTape assay in 2200 TapeStation System (Agilent Technologies ...
-
bioRxiv - Molecular Biology 2022Quote: ... The BAG6 target region was amplified from the genomic DNA from all samples using Q5 High-Fidelity Master Mix and subsequently purified using a NucleoSpin Gel and PCR Clean-up kit from Macherey-Nagel. The purified samples were sent for sanger sequencing and the proportion of BAG6 knockout cells in each sample was estimated using the ICE CRISPR analysis tool (https://www.synthego.com/products/bioinformatics/crispr-analysis).
-
bioRxiv - Microbiology 2023Quote: ... The final products were purified by gel extraction from a 1% gel with the NucleoSpin Gel and PCR clean-up kit (Macherey-Nagel) and verified by Sanger sequencing.
-
bioRxiv - Microbiology 2023Quote: ... The final products were purified by gel extraction from a 1% gel with the NucleoSpin Gel and PCR clean-up kit (Macherey-Nagel) and sent for Sanger sequencing.
-
bioRxiv - Microbiology 2023Quote: ... Amplified libraries were cleaned up after gel electrophoresis (selected size between 200 bp to 500 bp) by a NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) according to the manufacturer’s instructions for high percentage agarose gels.
-
bioRxiv - Molecular Biology 2023Quote: ... the beads were resuspended in NTI buffer for DNA purification using NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, #740609.250).
-
bioRxiv - Microbiology 2023Quote: ... Pooled libraries were concentrated by ethanol precipitation and purified by gel extraction of the corresponding library size using the NucleoSpin Gel and PCR Clean-up Mini kit (Macherey-Nagel). Libraries were sequenced using the MiSeq Reagent Kit v3 with 300-bp paired end reads on a MiSeq (Illumina).
-
bioRxiv - Microbiology 2023Quote: PCR product from multiple reactions was accumulated and gel extracted from a 0.8% (w/v) TAE agarose gel using a Nucleospin Gel and PCR clean-up kit (Macherey-Nagel, 740609.250). Gels were cut without exposing the DNA to UV radiation damage or stain ...
-
bioRxiv - Physiology 2023Quote: ... Products were run on 1% agarose gels and then gel-purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) according to manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... the DNA eluates were de-cross-linked at 65°C overnight with shaking at 900rpm and purified by NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel), according to the manufacturer’s instructions.
-
bioRxiv - Bioengineering 2023Quote: ... The PCR product was run on a 1% agarose gel and the correct band according to size was purified using the NucleoSpin Gel and PCR Cleanup kit (Macherey-Nagel) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2023Quote: ... and 16S amplicons (500 bp amplicon size) were purified by agarose gel separation using the NucleoSpin Gel and PCR Clean-up purification kit (Macherey-Nagel). Bacterial 16S amplifications were obtained using the FastStart High-Fidelity PCR system (Roche ...
-
bioRxiv - Microbiology 2023Quote: ... DNA purification from agarose gel or enzymatic reactions was performed with the NucleoSpin Gel and PCR clean up kit (Macherey-Nagel). PCRs were performed with Phusion DNA polymerase (Thermo Fisher Scientific) ...
-
bioRxiv - Microbiology 2023Quote: ... PCR-column purified (Macherey-Nagel), and sent for Sanger sequencing at Genewiz with the primers in Table 2.
-
bioRxiv - Physiology 2019Quote: ... checked with test strips for rapid detection of glucose (Medi-Test, Macherey-Nagel, Düren, Germany), respectively ...
-
bioRxiv - Genomics 2019Quote: ... PCR products were purified using NucleoSpin® Gel and PCR Clean-up (Macherey-Nagel #740609) and analyzed with capillary electrophoresis using an Agilent 2100 Bio-analyzer and an Agilent DNA 1000 kit (#5067-1504).
-
bioRxiv - Synthetic Biology 2020Quote: ... and the PCR products purified using the NucleoSpin Gel and PCR Clean-up (Macherey-Nagel). Indexing PCR and NGS were performed by Microsynth (Balgach) ...
-
bioRxiv - Cancer Biology 2021Quote: ... PCR amplicons were gel extracted using NucleoSpin Gel and PCR clean-up (Macherey-Nagel, Germany) kit according to manufacturer’s instructions and quantified using Nanodrop ...
-
bioRxiv - Genomics 2022Quote: ... and the PCR products were purified using NucleoSpin Gel and PCR Clean-up (MACHEREY-NAGEL) and then sequenced ...
-
bioRxiv - Microbiology 2023Quote: ... PCR products were purified from agarose gels (NucleoSpin Gel and PCR Clean-up, Macherey-Nagel). Nested PCR (position ...
-
bioRxiv - Cell Biology 2023Quote: ... Following PCR product purification (NucleoSpin Gel and PCR Clean-up, Macherey-Nagel GmbH, Düren, Germany), the DNA was sent for Sanger sequence analysis (Microsynth Seqlab GmbH ...