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Citations for Macherey-Nagel GmbH :
401 - 450 of 2222 citations for ssc mir 17 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... PCR products were purified from agarose gels (NucleoSpin Gel and PCR Clean-up, Macherey-Nagel). Nested PCR (position ...
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bioRxiv - Cell Biology 2023Quote: ... Following PCR product purification (NucleoSpin Gel and PCR Clean-up, Macherey-Nagel GmbH, Düren, Germany), the DNA was sent for Sanger sequence analysis (Microsynth Seqlab GmbH ...
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bioRxiv - Immunology 2021Quote: ... Positive clones were identified by colony PCR and the plasmids were isolated using NucleoSpin® Plasmid EasyPure kit (Macherey-Nagel, Düren, Germany). The DNA sequence of the cloned devil CIITA transcript was verified by Sanger sequencing using Big Dye™ Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems (ABI) ...
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bioRxiv - Neuroscience 2020Quote: ... Gel extraction was performed to select for products between 200 – 1000 bp using the Nucleospin Gel and PCR Clean-up kit (Macherey-Nagel, 740609). Samples were eluted in 11 μL NF H2O ...
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bioRxiv - Cell Biology 2021Quote: ... Eluates were digested with proteinase K and RNAse A and DNA fragments were purified using a PCR purification kit (Macherey-Nagel, #740609).
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bioRxiv - Developmental Biology 2021Quote: ... and the band with the expected size excised and purified using the NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel). The second PCR products were sent for Sanger sequencing to Eurofins Genomics and checked for quality ...
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bioRxiv - Genomics 2020Quote: ... Cleaved Histone-DNA complexes were isolated by centrifugation and DNA was extracted with a NucleoSpin PCR Clean-up kit (Macherey-Nagel, 740609).
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bioRxiv - Developmental Biology 2022Quote: ... plasmids were linearized by AscI (pBSK vector) or SfiI (pYX vector) and purified by NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, #740609). cRNA was produced by in vitro transcription using mMessage mMachine™ T3 kit (Ambion ...
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A conserved isoleucine in the binding pocket of RIG-I controls immune tolerance to mitochondrial RNAbioRxiv - Immunology 2022Quote: ... RNA bands according to the size of the 18s and 28s rRNA were cut from the agarose gel and extraction was performed using the NucleoSpin® Gel and PCR Clean-Up Kit (Macherey-Nagel). The extraction was performed according to the manufactureŕs protocol for RNA extractions from agarose gels using the buffer NTC (Macherey Nagel).
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bioRxiv - Cancer Biology 2023Quote: ... The PCR products from the same sample were pooled and purified over 2% agarose gels (band size at 275 bp) using NucleoSpin® Gel and PCR clean up kit (Macherey-Nagel). Purified amplicons were quantified by Qubit (Thermofisher ...
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bioRxiv - Microbiology 2023Quote: ... The remaining PCR reaction of the sample with gRNA–TAR and –p24 combined was run on a 1% agarose gel containing ethidium bromide and the two expected bands were cut out separately and purified using the Nucleospin® Gel and PCR Clean-up kit (Macherey-Nagel) according to the manufacturer’s manual ...
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bioRxiv - Molecular Biology 2023Quote: ... were then extracted from the agarose gel and the DNA purified following the manufacturer instruction (NucleoSpin® Gel and PCR Clean-up kit (MACHEREY-NAGEL)) ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR reaction was performed on the new synthetized vector to amplify the HA1-DCK*-P2A-GFP-HA2 sequence corresponding to the HDR (homology directed repair) DNA template and was purified using the NucleoSpin PCR Clean-up kit (Macherey-Nagel # 740609.10) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... The resulting amplicons were purified using the NucleoSpin gel and PCR cleanup kit following the manufacturer’s instructions (Macherey-Nagel Inc., Bethlehem, PA) and submitted for Sanger sequencing with the same primers used to generate the amplicons ...
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bioRxiv - Systems Biology 2020Quote: ... PCR products were pooled and purified by NucleoSpin Gel and PCR Clean-up columns (Macherey-Nagel) at 5 reactions per column ...
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bioRxiv - Neuroscience 2020Quote: ... PCR products were purified using NucleoSpin® Gel and PCR Clean-up (Macherey-Nagel, Hoerdt, France), according to manufacturer’s protocol.
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bioRxiv - Biochemistry 2021Quote: ... PCR products were cleaned using the NucleoSpin® Gel and PCR Clean-up (Macherey-Nagel, USA). The amplified products were cloned into pDrive using the TA Cloning Kit (Qiagen® ...
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bioRxiv - Genomics 2020Quote: ... DNA was purified with PCR clean up columns (NucleoSpin Gel and PCR Clean-up, Macherey-Nagel) and sequenced using Illumina technology.
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bioRxiv - Microbiology 2020Quote: ... The PCR products were purified using Nucleo Spin Gel and PCR Clean up (Macherey-Nagel, Germany). The cleaned-up PCR products were sent Macrogen (Europe ...
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bioRxiv - Microbiology 2023Quote: 3D-PCR products were purified from agarose gels (NucleoSpin Gel and PCR Clean-up, Macherey-Nagel) and ligated into the TOPO TA cloning vector (Invitrogen) ...
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Critical contribution of mitochondria in the development of cardiomyopathy linked to desmin mutationbioRxiv - Pathology 2023Quote: ... 3D-PCR products were purified from agarose gels (NucleoSpin Gel and PCR Clean-up, Macherey-Nagel) and ligated into the TOPO TA cloning vector (Invitrogen) ...
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bioRxiv - Developmental Biology 2024Quote: ... PCR product was then purified by following NucleoSpin PCR Clean-up procedure (Macherey-Nagel; Cat# 740609.250) and sent for Sanger sequencing to confirm genotypes.
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bioRxiv - Cell Biology 2023Quote: ... and PCR purified (Macherey-Nagel, 740609.50). The two purified fragments were then ligated using T4 DNA ligase (NEB ...
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bioRxiv - Microbiology 2021Quote: ... was linearized by AdeI restriction enzyme digestion and DNA was purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany). For in-vitro transcription ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and products of the expected size were cut out and purified using NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel, Duren, Germany). 10 ng of purified PCR product was then used as template for RNA probe synthesis ...
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bioRxiv - Molecular Biology 2020Quote: ... The PCR products were analysed using 1.5% agarose gel electrophoresis in 1x Tris-borate-EDTA (TBE) buffer and purified from the gel using a NucleoSpin® gel and PCR clean-up kit (Macherey-Nagel, Germany).
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bioRxiv - Microbiology 2019Quote: ... and analyzed and purified using an agarose gel with subsequent gel extraction with the “PCR clean-up and Gel extraction” Kit from Macherey-Nagel (Düren, Germany). A total of 90 ng of DNA per lane was incubated with different molar excesses of purified MalR protein (3-fold and 10-fold molar excess ...
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bioRxiv - Immunology 2020Quote: ... The reactions were then run at 100V in a 1% agarose gel and the digest vectors were excised and purified using a NucleoSpin® Gel and PCR Clean-up Kit (Macherey-Nagel, USA) according to instructions set by the manufacturer ...
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bioRxiv - Synthetic Biology 2022Quote: ... Cat#M0491S/L) and gel extractions were done with the Macherey-Nagel NucleoSpin Gel and PCR Clean-up Mini Kit (Macherey-Nagel, Cat#740609). DNA assemblies were carried out by In-Fusion Cloning (Takara Bio ...
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bioRxiv - Plant Biology 2022Quote: ... the rhizosphere and root subpools were purified on an agarose gel (amplicon ∼450 bp) using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany), whereas all other subpools were purified with CleanNGS beads ...
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bioRxiv - Genomics 2022Quote: ... The DNA band at 5 to 10kb size was collected for extraction with a NucleoSpin Gel and PCR Clean-up purification kit following the manufacturer’s instructions (Macherey-Nagel, cat# 740609.250, Germany), and was eluted with 15 μl NF water.
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bioRxiv - Genomics 2021Quote: ... PCR reactions were pooled and purified on NucleoSpin Gel and PCR Clean-up #740609 columns (Macherey-Nagel). The screening vector was linearized with AgeI-HF and SalI-HF restriction enzymes (NEB ...
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bioRxiv - Systems Biology 2020Quote: ... The PCR products were pooled and purified with NucleoSpin Gel and PCR Clean-up columns (Macherey-Nagel) at 20 reactions per column ...
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bioRxiv - Biochemistry 2021Quote: ... purified PCR fragment (purified using Nucleospin® Gel and PCR Clean-up, MACHEREY-NAGEL GmbH & Co. KG), and H2O to acquired final volume ...
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bioRxiv - Cell Biology 2021Quote: ... PCR fragments and plasmids were respectively purified with NucleoSpin® Gel and PCR cleanup (Macherey-nagel # 740609) and NucleoSpin® Plasmid (Macherey-Nagel #740499) ...
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bioRxiv - Neuroscience 2020Quote: ... Genotyping for the tm1a cassette was performed using gene-specific polymerase chain reaction (PCR) on DNA extracted from ear tissue using a tissue DNA extraction kit (Macherey-Nagel, Bethlehem, PA, USA) with primers suggested by the stock facility (forward ...
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bioRxiv - Systems Biology 2020Quote: ... with ALLinTM HiFi DNA Polymerase (HighQu, Kraichtal, Germany) and purified with the NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany). Plasmids were constructed from PCR-generated fragments and pET16b vector cut with restriction enzymes using the isothermal DNA assembly method (48) ...
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bioRxiv - Microbiology 2020Quote: ... The final PCR product from the 5’ RACE procedure was excised from a 1% agarose gel and purified by column capture (Macherey-Nagel Nucleospin mini kit). The purified product was then Sanger sequenced (Eurofins Scientific ...
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bioRxiv - Microbiology 2021Quote: ... The DNA product was verified by gel electrophoresis and purified with the NuceloSpin Gel and PCR Clean-up Kit (Macherey-Nagel, Cat. No. 740609.50). The concentration of the purified PCR product was adjusted to 5 ng/μl in 15 μl and send to Sanger sequencing by Eurofins Genomics ...
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bioRxiv - Neuroscience 2021Quote: ... Genotyping for the tm1a cassette was performed using gene-specific polymerase chain reaction (PCR) on DNA extracted from ear tissues with a tissue DNA extraction kit (Macherey-Nagel, Bethlehem, PA, USA) with primers (forward 5’ to 3’ ...
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bioRxiv - Microbiology 2022Quote: ... The product of barcoding qPCR was cleaned by gel extraction using NucleoSpin Gel and PCR clean-up kit (MACHEREY-NAGEL, GmbH & Co, KG, Germany), after 1% AGE electrophoresis run for 1 hour at 100 V ...
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bioRxiv - Microbiology 2023Quote: ... a 450 bp long sequence upstream of timA was synthesized by PCR from EGD-e genomic DNA using primers TE74 and TE75 and purified using the NucleoSpin® Gel and PCR Clean-up Kit (Macherey-Nagel, Düren, Germany). For the EMSA experiment ...
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bioRxiv - Genetics 2023Quote: ... PCRs for loci of interest were run using Amplicon-EZ (Genewiz) partial IlluminaÒ adapters and amplicons were processed using NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel, Catalog no. 740609.250). Paired end (2 x 250 bp ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The obtained PCR products were purified using NucleoSpin Gel and PCR Clean-up (Macherey-Nagel GmbH, Düren, Germany) according to supplier’s instructions ...
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bioRxiv - Synthetic Biology 2020Quote: ... PCR products from the first reaction were purified with NucleoSpin Gel and PCR Clean-up (Macherey-Nagel, Germany) and used (1 µL ...
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bioRxiv - Bioengineering 2022Quote: ... DNA was purified from PCR mixtures or agarose gels using NucleoSpin Gel and PCR Clean-up (Macherey-Nagel). The purity and concentration of DNA were checked by NanoDrop 2000 (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2020Quote: ... PCR products were purified (MACHEREY-NAGEL, 740609.250), digested with the appropriate restriction enzymes (New England Biolabs) ...
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bioRxiv - Microbiology 2023Quote: ... PCR amplicons were purified (Macherey-Nagel #740609) and sent for Sanger sequencing (Eurofins genomics) ...
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bioRxiv - Cancer Biology 2021Quote: ... Final amplicons from duplicate internal PCRs were gel extracted using NucleoSpin Gel and PCR clean-up (Macherey-Nagel, Germany) kit according to manufacturer’s instructions and quantified using Nanodrop ...
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bioRxiv - Plant Biology 2020Quote: ... PCR products were purified to remove unincorporated nucleotides and primers using NucleoFast® 96 PCR plates (Macherey-Nagel, Oensingen, Switzerland). Details of the annealing temperatures and primers were published previously (Friesen et al ...