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Citations for Macherey-Nagel GmbH :
101 - 150 of 2219 citations for rno mir 542 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... The resulting PCR product was ‘cleaned up’ using a NucleoSpin Gel and PCR clean-up kit (Macherey-Nagel). Capped and Poly(A ...
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bioRxiv - Microbiology 2019Quote: ... PCR products were then purified using the NucleoSpin® PCR Clean-up kit (MACHEREY-NAGEL, GmbH & Co. KG), quantified with Qubit dsDNA HS Assay kit and cloned using the pGEM®-T Easy Vector Systems (Promega ...
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bioRxiv - Cell Biology 2020Quote: ... The products of the second PCR were purified with a NucleoSpin 96 PCR Clean-up kit (Macherey-Nagel), lyophilized ...
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bioRxiv - Microbiology 2021Quote: ... the PCR products were purified using NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany). Purified PCR products were cloned into pGEM®-T Easy Vector (Promega ...
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bioRxiv - Genetics 2021Quote: ... The PCR amplicon was gel-purified by using the Nucleospin Gel and PCR Clean-up kit (Macherey-Nagel) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... PCR products were purified from agarose gels using the Nucleospin Gel and PCR Clean-Up kit (Macherey-Nagel). EIF2AK2 and EIF2S1 sequences were inserted into pET-His 1a vector using the Gibson Assembly Cloning Kit (New England Biolabs ...
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bioRxiv - Microbiology 2019Quote: ... The 8kb PCR fragment was gel extracted using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) and eluted in P3 Buffer (Lonza ...
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bioRxiv - Molecular Biology 2023Quote: ... The PCR products were purified by a NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel., Düren, Germany). COI gene fragments were amplified from some samples according to the USDA recommended protocol (https://www.fsis.usda.gov/sites/default/files/media_file/2020-11/CLG-FPCR.pdf ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR products were evaluated by electrophoresis and then further processed with the PCR clean-up Kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were subsequently purified using the NucleoSpin Gel and PCR Clean-up kit (MACHEREY-NAGEL, Düren, Germany). Plasmids were purified using the QIAprep Spin Miniprep Kit (QIAGEN ...
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bioRxiv - Bioengineering 2023Quote: ... PCR samples were then purified using following the manufacturer’s instructions (NucleoSpin® PCR and gel kit, Macherey-Nagel). Purified amplicons were verified by electrophoresis on a 1% agarose gel ...
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bioRxiv - Genetics 2023Quote: ... the fragments were purified using a PCR/Gel purification kit (NucleoSpin Gel and PCR Clean□up, Macherey-Nagel) and sequenced using the smt0-seq-Rv oligonucleotide ...
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bioRxiv - Microbiology 2024Quote: ... and the resultant PCR product gel extracted using a NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). flaB promoter-lacI and pQE30 were Q5 PCR-amplified from pJSB252 using primers PA468 + PA471 (for pind template ...
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bioRxiv - Microbiology 2024Quote: ... DNA fragments and PCR products were purified using the Nucleospin Gel and PCR clean up kit (Macherey-Nagel).
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bioRxiv - Bioengineering 2020Quote: ... NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel, Germany) was used for DNA clean-up both from PCR products and agarose gel extracts ...
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted using Nucleospin PCR clean-up kit (Macherey-Nagel) and resuspended in 50 μl elution buffer (5 mM Tris-HCl pH 8.5) ...
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bioRxiv - Microbiology 2022Quote: ... DNA was extracted using a Nucleospin PCR cleanup kit (Macherey-Nagel) and resuspended in 50 μl elution buffer (5 mM Tris-HCl [pH 8.5]) ...
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bioRxiv - Microbiology 2021Quote: ... using the NucleoSpin Gel and PCR clean-up kit (Macherey-Nagel). Gel-purified amplicons were then assembled using NEBuilder HiFi DNA Assembly Master Mix at a 1:1 molar ratio for each DNA fragment and incubated at 50°C for two hours ...
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bioRxiv - Plant Biology 2022Quote: ... purified using NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel), and circularized using T4 DNA ligase.
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bioRxiv - Synthetic Biology 2022Quote: A NucleoSpin PCR clean-up gel extraction kit (Macherey-Nagel, Germany) was used to purify PCR products before their use in cloning ...
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bioRxiv - Microbiology 2022Quote: ... NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel, Bethlehem, PA) was used to remove contaminants ...
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bioRxiv - Molecular Biology 2023Quote: ... or the NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... or the Nucleospin gel and PCR clean-up kit (Macherey-Nagel). Restriction and ligation reactions were performed using enzymes and buffers obtained from New England Biolabs and Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... or the NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel). All constructed plasmids were sequenced by Microsynth AG (Balgach ...
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bioRxiv - Microbiology 2024Quote: ... DNA was extracted using a Nucleospin PCR cleanup kit (Macherey-Nagel) and resuspended in 50 μl elution buffer (5 mM Tris-HCl [pH 8.5]) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the PCR products were purified using a NucleoSpin Gel and a PCR Clean-up Kit (Macherey-Nagel, Duren, Germany) and then sequenced directly by Sanger sequencing using the same PCR primers ...
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bioRxiv - Genomics 2021Quote: ... PCR was performed and the products were purified with a NucleoSpin Gel and PCR Clean-up kit (MACHEREY-NAGEL).
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bioRxiv - Microbiology 2022Quote: ... PCR products were purified using the NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel™, Düren, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... the adeD PCR product was additionally gel-purified (NucleoSpin Gel and PCR Clean-Up Kit, Macherey-Nagel, Düren, DE), and 100 ng of purified DNA was subsequently incubated with 0.2 M dATP and 5 U DreamTaq Polymerase (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... Resulting PCR products were purified from 1% agarose gels with NucleoSpin Gel and PCR Clean-Up Kit (Macherey-Nagel) to prevent the production of side products during in vitro transcription ...
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bioRxiv - Molecular Biology 2022Quote: ... All PCR fragments for cloning and plasmids were purified using NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) and GenElute™ Plasmid Miniprep Kit (Merck) ...
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bioRxiv - Molecular Biology 2022Quote: ... Certain PCR products were purified from the gel using NucleoSpin Gel and PCR Clean-up Mini kit (Macherey-Nagel) and cloned individually in a TA-cloning vector called pGEM®-T Easy Vector (Promega ...
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bioRxiv - Microbiology 2024Quote: ... Total RNA for quantitative reverse transcription PCR (qRT-PCR) was isolated using the NucleoSpin® RNA Kit (Macherey-Nagel), including DNA digestion ...
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bioRxiv - Microbiology 2024Quote: ... The resulting PCR product was purified using the Nucleospin Gel and PCR clean-up kit (Macherey-Nagel Cat#740609.50), and resolved on a 8% polyacrylamide TBE gel (Life Technologies Cat#EC62162BOX) ...
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bioRxiv - Molecular Biology 2019Quote: ... the PCR product was purified with NucleoSpin Gel and PCR clean-up kit according to manufacturer’s description (Macherey-Nagel, 740609). RF cloning into pET28a was performed with eighteen 3-step cycles with annealing temperature 65°C and extension time 7 minutes ...
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bioRxiv - Genomics 2019Quote: ... PCR products were checked by agarose gel electrophoresis and purified using Nucleospin Gel and PCR clean-up kit (Macherey-Nagel). Cycle sequencing reactions were performed using the BigDye 3.1 chemistry ...
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bioRxiv - Developmental Biology 2021Quote: ... PCR products were cleaned up using Macherey-Nagel™ NucleoSpin™ Gel and PCR Clean-up Kit (Macherey-Nagel, 740609.50) and sequenced using Sanger Sequencing (Department of Biochemistry ...
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bioRxiv - Developmental Biology 2021Quote: ... PCR products were cleaned up using Macherey-Nagel™ NucleoSpin™ Gel and PCR Clean-up Kit (Macherey-Nagel, 740609.50) and sent for Sanger Sequencing (Department of Biochemistry ...
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bioRxiv - Microbiology 2019Quote: ... PCR products were then purified using a Millipore NucleoFast 96 PCR kit following the manufacturer’s recommendations (Macherey-Nagel, Düren, Germany) and sequenced using the BigDye Terminator Cycle Sequencing Kit (Applied Biosystems ...
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bioRxiv - Microbiology 2019Quote: ... and the resulting PCR products were cleaned using a NucleoSpin Gel and PCR Clean-Up kit (Macherey-Nagel, Düren, Germany). Samples were prepared for sequencing using a BigDye Terminator 3.1 kit (Thermo Fisher ...
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bioRxiv - Microbiology 2022Quote: ... and purified directly from the PCR mix or from an agarose gel (Gel and PCR clean-up kit, Macherey-Nagel); the two fragments were fused together thanks to a primer-encoded 20-30bps overlap during a second PCR amplification round and the product cloned into the pEMG suicide vector [20] using EcoRI and BamHI ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were subjected to a final gel purification using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel).
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bioRxiv - Molecular Biology 2019Quote: ... PCR products were recovered and purified using a NucleoSpin® Gel and PCR Clean-up Kit (Macherey-Nagel, Düren, DE). Concentrations of purified PCR products were determined by absorbance measurements at a wavelength of 260 nm using an infinite F200 pro device (TECAN ...
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bioRxiv - Biochemistry 2023Quote: ... The PCR products were purified using the NucleoSpin Gel and PCR Clean-up Mini Kit (Macherey-Nagel, catalog no. 740609.50) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR amplicons were subjected to agarose gel size selection using the NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel). Amplicons were cloned into BsmBIv2-digested (New England BioLabs ...
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bioRxiv - Plant Biology 2021Quote: ... PCR products were purified by agarose electrophoresis and NucleoSpin kit (Macherey-Nagel). 1µg of purified DNA was used for in-vitro transcription following the manufacturer instructions (HiScribe T7 High Yield RNA Synthesis Kit ...
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bioRxiv - Microbiology 2020Quote: ... followed by the NucleoSpin Gel and PCR clean up kit (Macherey-Nagel) and sent to Eurofins (Ebersberg ...
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bioRxiv - Molecular Biology 2020Quote: ... the samples were purified with PCR clean-up kit from MACHEREY-NAGEL and eluted in 50 μL of elution buffer.
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bioRxiv - Evolutionary Biology 2021Quote: ... purified with a PCR clean-up and gel extraction kit (Macherey-Nagel), and quantified with the Kapa Library Quantification Kit (Kapa Biosystems) ...
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bioRxiv - Plant Biology 2021Quote: ... We used the NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel) for band purification and sequencing was performed by Eurofins Genomics.