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Citations for Macherey-Nagel GmbH :
301 - 350 of 2219 citations for rno mir 143 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... and DNA was purified using the NucleoSpin® Gel and PCR Clean-Up Kit (Macherey-Nagel; #740609.50) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2019Quote: Total RNA was extracted from 50 to 250 EB collected at different time points using the NucleoSpin® RNA kit (Macherey-Nagel) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: Cells pellet (1.5*10^6) were collected at different time points after LPS stimulation and DNA was extracted with the Nucleospin tissue kit (Macherey-Nagel, 740952). The genomic DNA was eluted in 50 μl of BE buffer (5 mM Tris/HCl ...
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted from 1 mL co-culture taken from different time points during the cultivation experiment using the NucleoSpin Tissue DNA extraction kit according to the manufacturer’s instructions (Macherey-Nagel, Düren, Germany). Cell numbers were quantified by direct epifluorescence microscopy of Sybr-green stained cells immobilized on agarose-coated slides to generate standard curves for qPCR as described previously [42] ...
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bioRxiv - Molecular Biology 2022Quote: ... Reverse cross-linked chromatin was purified using the NucleoSpin™ Gel and PCR Clean-up kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... The dsDNA donors were then column-purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) and stored at -20°C or directly used for electroporation ...
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bioRxiv - Biochemistry 2019Quote: ... The eluted DNA samples were further purified with a NucleoSpin Gel and PCR clean-up kit (MACHEREY-NAGEL). The DNA libraries were prepared using a SMARTer ThruPLEX Tag-seq Kit (Takara Bio) ...
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bioRxiv - Genetics 2020Quote: ... the single band was purified from a gel with a NucleoSpin Gel and PCR cleanup kit (Macherey-Nagel) and its concentration determined ...
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bioRxiv - Synthetic Biology 2021Quote: Gel-purify the excised band using the Macherey-Nagel NucleoSpin Gel & PCR Clean-up kit (Macherey-Nagel, Germany). Insert the purified array into the opened plasmid backbone using In-Fusion cloning (Takara Bio ...
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bioRxiv - Synthetic Biology 2021Quote: ... DNA fragments were purified with a NucleoSpin™ Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany). Restriction enzymes and T4 DNA ligase were obtained from Thermo Fisher Scientific and were used according to the supplier’s specifications ...
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bioRxiv - Microbiology 2023Quote: ... DNA fragments used in cloning reactions were purified using the NucleoSpin gel and PCR cleanup kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... The DNA donors were then column-purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) and stored at -20°C or directly used for electroporation ...
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bioRxiv - Molecular Biology 2023Quote: ... and the reverse transcription products were purified using a NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). The reverse transcription products were eluted with 30 μL of HiDi formamide (Thermo Fisher) ...
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bioRxiv - Microbiology 2023Quote: ... and the backbone band was cut out and isolated using NucleoSpin Gel and PCR cleanup Kit (Macherey-Nagel). Backbone and insert were ligated using T4 Ligase (Thermo Fisher ...
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bioRxiv - Molecular Biology 2023Quote: ... Amplicons were purified via gel extraction using a NucleoSpin Gel and PCR Clean-up Mini kit (Macherey-Nagel). Illumina sequencing adapters were added with Phusion High-Fidelity DNA Polymerase (NEB ...
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bioRxiv - Immunology 2023Quote: ... purified again via agarose gel separation and using NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Germany), before being pooled and submitted to GATC Biotech (Germany) ...
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bioRxiv - Microbiology 2023Quote: ... and DNA bands of interest were isolated using the NucleoSpin® Gel & PCR Cleanup Kit from Macherey-Nagel. The extracted DNA underwent Sanger sequencing at Eurofins Scientific ...
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bioRxiv - Genetics 2023Quote: ... All probes were gel purified using the Nucleospin Gel and PCR Clean-up kit (Macherey-Nagel, cat# 740609). The pixel intensities of the bands corresponding to linear ...
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bioRxiv - Microbiology 2024Quote: ... RNA was column purified using NTC buffer and the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel), according to the manufacturer’s instructions.
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bioRxiv - Plant Biology 2020Quote: ... Total RNA was isolated from leaves harvested at the indicated time point using the NucleoSpin® RNA Plus kit (Macherey-Nagel, Düren, Germany). cDNA was synthesized from 2 μg of total RNA using the RevertAid First Strand cDNA Synthesis Kit (Thermo Scientific ...
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ADEVO: Proof-of-concept of Adenovirus Directed EVOlution by random peptide display on the fiber knobbioRxiv - Bioengineering 2023Quote: ... infected cells were washed five times to eliminate non-internalized VPs and DNA was extracted using the NucleoSpin Tissue kit (Macherey-Nagel #740952-250). Internalized vector genomes were titrated by qPCR using a forward primer in the U exon ...
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bioRxiv - Molecular Biology 2021Quote: ... The amplicons were purified using the NucleoSpin® Gel and PCR clean up kits from Macherey-Nagel (Bethlehem, PA), after which quantification and qualification of purified band(s ...
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bioRxiv - Biochemistry 2019Quote: ... purified according to the instructions of the NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel GmbH, Düren, Germany) and cloned into the pET22b(+ ...
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bioRxiv - Biochemistry 2020Quote: ... Immunoprecipitated DNA was purified with NucleoSpin Gel and PCR Clean-up Kit (MACHEREY-NAGEL GmbH & Co. KG, Düren, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Digested DNA was cleared from restriction enzymes using the NucleoSpin Gel and PCR Clean-up kit (740609.250, Macherey-Nagel). Insert-DNA (50 ng ...
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bioRxiv - Biochemistry 2020Quote: ... After gel extraction of the DNA fragments with the PCR Clean-up Gel Extraction Kit (Macherey-Nagel, Düren, Germany), the DNA fragments and the vector DNA were digested by the same restriction enzymes (Supplemental Table S4 ...
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bioRxiv - Microbiology 2020Quote: ... ChIP-DNA and input DNA were purified using the NucleoSpin® Gel and PCR Clean-up Kit (Macherey-Nagel). The DNA concentration of the input DNA was determined using the Qubit dsDNA BR Assay Kit (Thermo Fisher Scientific).
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bioRxiv - Biochemistry 2021Quote: ... the band containing the efrCD genes were extracted with the NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel).
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bioRxiv - Molecular Biology 2021Quote: ... DNA fragments were purified from agarose gels using the NucleoSpin Gel and PCR Clean-up kit from Macherey-Nagel. Genomic DNA extractions from Amycolatopsis and E ...
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bioRxiv - Microbiology 2020Quote: ... difficile strains was estimated by quantitative PCR on genomic DNA extracted using the NucleoSpin Microbial DNA kit (Macherey-Nagel). The total chromosome copy number was quantified based on the reference gene dnaF (CD1305 ...
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bioRxiv - Molecular Biology 2023Quote: ... and linearized plasmid was purified from the gel using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). In-Fusion reaction was prepared as follows ...
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bioRxiv - Immunology 2023Quote: ... The excised DNA fragments were further refined using nucleospin gel and by using PCR clean-up kit (Macherey-Nagel) and quantified for determining the purity and concentration ...
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bioRxiv - Molecular Biology 2023Quote: ... The linearised fragment was then excised and purified using the NucleoSpin Gel and PCR Clean up Kit (Macherey-Nagel). 400nM of the annealed oligos were ligated with lng of digested crRNA backbone using T4 ligase (Promega ...
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bioRxiv - Genomics 2023Quote: A second purification step was performed using a PCR product extraction from gel agarose kit from Macherey-Nagel (MN) NucleoSpin® Gel and PCR Clean-up kit and restarting the above protocol from the step with the addition of CQW buffer then PW2 buffer.
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bioRxiv - Microbiology 2024Quote: ... All positive samples were purified with the NucleoSpin Gel and PCR Clean-up kit by Macherey-Nagel (ref: 740609.50S). Briefly ...
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bioRxiv - Microbiology 2024Quote: ... Each mix was then purified using the NucleoSpin Gel and PCR Clean-up kit by Macherey-Nagel (ref: 740609.50S). DNA was then eluted and quantified at a Nanodrop 2000 Spectrophotometer (ThermoScientific) ...
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bioRxiv - Cell Biology 2024Quote: ... the first-strand cDNA was purified with the NucleoSpin gel and PCR clean-up kit (Macherey-Nagel, no. 470609) following the manufacturer’s instruction ...
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bioRxiv - Plant Biology 2020Quote: PCR products were purified by NucleoFast 96 PCR plates (Macherey-Nagel, Germany) and were sequenced directly on both DNA strands on Applied Biosystems (Weiterstadt ...
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bioRxiv - Cell Biology 2022Quote: ... PCR products were purified (NucleoSpin Gel and PCR Clean-up, Macherey-Nagel) and used as templates for T3 and T7 transcription reactions (MEGAscript ...
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bioRxiv - Genetics 2024Quote: RNA was extracted from a total of 24 samples of male brain (four vgll3*EE and four vgll3*LL individuals for each of the three time points) using a NucleoSpin RNA kit (Macherey-Nagel GmbH & Co. KG). The collected samples were transferred to tubes containing 1.4 mm ceramic beads from Omni International ...
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bioRxiv - Cancer Biology 2020Quote: ... treatment with 100µg/ml RNase A (37° for 15 minutes) and 50µg/ml proteinase K and purification using the PCR cleanup kit (Macherey-Nagel).
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bioRxiv - Plant Biology 2019Quote: ... Size selection on each pool was performed via gel extractions using NucleoSpin Gel and PCR Clean-up kit (MACHEREY-NAGEL) targeting fragment sizes comprised between 250 and 500 bp ...
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bioRxiv - Immunology 2020Quote: ... Positive clones were identified by colony PCR and the plasmid was purified using NucleoSpin® Plasmid EasyPure kit (Macherey-Nagel). The cloned devil NLRC5 transcript was verified by Sanger sequencing using Big Dye™ Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems (ABI) ...
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bioRxiv - Plant Biology 2019Quote: ... DNA from the IP and input fractions was purified using the Nucleospin Gel and PCR clean-up kit (Macherey-Nagel). DNA from IP and input were used for library preparation and sequencing by Illumina Hi-Seq (FASTERIS ...
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bioRxiv - Developmental Biology 2021Quote: ... The DNAs have been extracted on an agarose gel and purified (using the PCR clean-up gel extraction kit #740609.50, Macherey-Nagel) and the sanger sequencings have been performed by Eurofins ...
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bioRxiv - Genetics 2020Quote: ... Obtained libraries were pooled in equimolar amounts and purified using NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel), followed by a second purification step of gel extraction and size exclusion with AMPure XP magnetic beads (Beckman Coulter) ...
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bioRxiv - Microbiology 2022Quote: ... The amplicons were purified by the NucleoSpin Gel and PCR cleanup kit according to manufacturer’s instructions (Macherey-Nagel, Dueren, Germany). Purified products were sent for Sanger sequencing with primers rbcL404+ and rbcL587-to Macrogen (http://dna.macrogen-europe.com).
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bioRxiv - Biochemistry 2023Quote: ... Digestion products were isolated on agarose gels and purified with the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). Plasmid isolation was performed with NucleoSpin Plasmid kit (Macherey-Nagel) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The 140-bp UPTAG and DNTAG libraries were purified using the NucleoSpin Gel and PCR clean-up kit (Macherey-Nagel), quantified using the Qubit dsDNA HS kit (ThermoFisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: PCR products were purified using NucleoSpin PCR Clean-up procedure (Macherey-Nagel 740609.250) and mutations were confirmed by sequencing ...